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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII"

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High-throughput hyperspectral microscopy

High-throughput hyperspectral microscopy

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xiii

作者： Gehm, M. E. Brady, D. J. Duke Univ Fitzpatrick Ctr Photon Durham NC 27708 USA

ISBN: (纸本)0819461326

A hyperspectral imager provides a 3-D data cube in which the spatial information (2-D) of the image is complemented by spectral information (1-D) about each spatial location. A static, high-throughput spectrometer design previously developed by our group can be used as the spectral engine in a high-throughput hyperspectral imager that avoids the Fourier undersampling issues present in previous dispersive designs. We present the theory for both pushbroom and tomographic operation and describe experimental results from our proof-of-concept implementation of a hyperspectral microscope.

关键词： hyperspectral imaging multidimensional microscopy

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Dynamic structured illumination microscopy: focused imaging and optical sectioning for moving objects

Dynamic structured illumination microscopy: focused imaging ...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xiii

作者： Krzewina, Leo G. Kim, Myung K. Univ S Florida Dept Phys Tampa FL 33620 USA

ISBN: (纸本)0819461326

Structured illumination microscopy (SIM) is a valuable tool for three-dimensional microscopy and has numerous applications in bioscience. Its success has been limited to static objects, though, as three sequential image acquisitions are required per final processed, focused image. To overcome this problem we have developed a multicolored grid which when used in tandem with a color camera is capable of performing SIM with just a single exposure. images and movies demonstrating optical sectioning of three-dimensional objects are presented, and results of applying color SIM for wide-field focused imaging are compared to those of SIM. From computer modeling and analytical calculations a theoretical estimate of the maximum observable object velocity in both the lateral and axial directions is available, implying that the new method will be capable of imaging a variety of live objects. Sample images of the technique applied to lens paper and a pigeon feather are included to show both advantages and disadvantages of CSIM.

关键词： three-dimensional microscopy microscopy optical sectioning structured illumination

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image enhancement for digital visualization of three dimensional integral imaging

Image enhancement for digital visualization of three dimensi...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xiii

作者： Ponce-Diaz, Rodrigo Alvarez-Elizondo, Martha Saenz, Julia Javidi, Bahram Inst Tecnol & Estudios Super Monterrey Av Eugenio Garza Sada 2501CP 64849 Monterrey NL Mexico Univ Connecticut Storrs CT 06269 USA

ISBN: (纸本)0819461326

In this work, we are proposing the use of digital image enhancement in three dimensional (3D) Integral Imaging (InI) applying in small objects visualization. We used a well known image filter algorithm for enhance the edges and detail information of the 3D reconstruction InI image via unsharp masking. Small objects as bugs were recorded in an elemental image array, image processed and digital reconstructed as 3D objects. We implemented the algorithm over the elemental image array as usual and using an innovative technique that involves a simple digital reconstruction algorithm by quadruple pixel extraction. Digital results show an improvement in details visualization, which have potential application in 3D microscopy.

关键词： integral imaging image enhancement three dimensional processing

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three-dimensional measurement of microorganism by retardation modulated differential interference contrast microscope

Three-dimensional measurement of microorganism by retardatio...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xiii

作者： Aoki, G. Itoh, M. Yasuno, Y. Yatagai, T. Univ Tsukuba Inst Appl Phys 1-1-1 Tennodai Tsukuba Ibaraki 3958573 Japan

ISBN: (纸本)0819461326

We propose a new technique for obtaining three-dimensional phase distribution on differential interference contrast microscope to modulate relative phase retardation between two shear beams. Using partial coherent theory we extract the phase information from two different retardation images. For the object in a weak phase region, simple formula is derived. The images of nematomorph were obtained in vivo.

关键词： differential interference contrast retardation modulated phase measurement microscope Nomarski

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Adaptive optics for three-dimensional optical data storage and micromachining

Adaptive optics for three-dimensional optical data storage a...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xiii

作者： Booth, Martin J. Schwertner, Michael Wilson, Tony Univ Oxford Dept Engn Sci Parks Rd Oxford OX1 3PJ England

ISBN: (纸本)0819461326

Many high resolution optical methods are affected by the presence of optical aberrations. These include microscopy, three-dimensional optical data storage and optical micromachining. We investigate the use of adaptive aberration correction applied to these techniques. In particular, it is shown how a deformable membrane mirror can be used to correct the aberrations when focusing deep into a multilayer optical data storage medium for both recording and read-out of data. Aberration correction for optical micromaching deep inside a substrate is also demonstrated.

关键词： Aberration correction Optical storage Monochromatic aberrations Adaptive optics Micromachining Microscopes 3D optical data storage Confocal microscopy Data storage Objectives

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3D deconvolution of adaptive-optics corrected retinal images

3D deconvolution of adaptive-optics corrected retinal images

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xiii

作者： Chenegros, G. Mugnier, L. M. Lacombe, F. Glanc, M. Off Natl Etud & Rech Aerosp Dept Opt BP 72 F-92322 Chatillon France Mauna Kea Technol F-75010 Paris France Lab Etudes Spatiales Instrument Astrophys Paris France

ISBN: (纸本)0819461326

We report on a deconvolution method developed in a Bayesian framework for adaptive-optics corrected images of the human retina. The method takes into account the three-dimensional nature of the imaging process;it incorporates a positivity constraint and a regularization metric in order to avoid uncontrolled noise amplification. This regularization metric is designed to simultaneously smooth noise out and preserve edges, while staying convex in order to keep the solution unique. We demonstrate the effectiveness of the method, and in particular of the edge-preserving regularization, on realistic simulated data.

关键词： image restoration deconvolution three-dimensional imaging adaptive optics retinal imaging microscopy

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Quasi-confocal fluorescence sectioning with dynamic speckle illumination

Quasi-confocal fluorescence sectioning with dynamic speckle ...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xiii

作者： Ventalon, Cathie Mertz, Jerome Boston Univ Dept Biomed Engn 44 Cummington St Boston MA 02215 USA

ISBN: (纸本)0819461326

We present a new fluorescence microscopy technique that provides depth discrimination in thick tissue. The technique relies on a simple modification to a conventional wide-field microscope, and consists in illuminating the sample with a sequence of speckle patterns and displaying the RMS or the variance of the resultant sequence of fluorescent images. We demonstrate quasi-confocal optical sectioning with an axial resolution of 5 microns FWHM. The lateral resolution is identical to the widefield microscope, namely 0.6 micron FWHM. images of a mouse brain are compared with standard wide-field images, demonstrating an efficient quasi-sectioning capacity in thick tissue throughout approximatively 100 microns depth. This is achieved because of the high contrast maintained by speckle in a scattering media.

关键词： fluorescence microscopy optical sectioning 3D imaging linear microscopy confocal microscopy speckle structured light illumination thick tissue imaging

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Use of bessel beams and position-sensitive detectors in scanning photon microscope for improved field depth and contrast

Use of bessel beams and position-sensitive detectors in scan...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xiii

作者： Khmaladze, Alexander Kim, Myung K. Univ S Florida Dept Phys 4202 E Fowler Ave Tampa FL 33620 USA

ISBN: (纸本)0819461326

The scanning photon microscope technique is a method of microscopic image formation that employs a laser beam focused on a sample, while non-imaging detector receives the scattered light. The scans are achieved by means of a galvanometer based scanning mirror and a motorized micrometer. The system produces images analogous to the scanning electron microscopy with three-dimensional effects of shadowing and reflection. Compared to a conventional wide-field imaging system, the method allows for a greater ease of operation and flexibility, as the image quality is dependent upon the characteristics of the laser beam, rather than imaging optics. The image resolution on the order of a micron is demonstrated. A further gain in terms of resolution and the depth of focus by employment of Bessel rather than Gaussian beams is discussed. Additionally, we used a position-sensitive quadrant photodiode detector to highlight the overall spatial orientation of the imaged surface as well as its roughness. This concept can be useful in many areas, such as coherence imaging and fluorescence.

关键词： scanning microscopy axicon surface profile

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Investigation of 3D microscopy using intensity diffraction tomography

Investigation of 3D microscopy using intensity diffraction t...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xiii

作者： Anastasio, Mark A. Huang, Yin Gbur, Greg Carney, P. Scott IIT Dept Biomed Engn Chicago IL 60616 USA Univ North Carolina Charlotte Dept Phys & Opt Sci Charlotte NC USA Univ Illinois Dept Elect & Comp Engn Urbana IL USA

ISBN: (纸本)0819461326

Diffraction tomography (DT) is an established imaging technique for reconstructing the complex-valued refractive index distribution of a weakly scattering 3D sample. Due to experimental difficulties associated with the direct measurement of the phase of an optical wavefield, the effectiveness of DT for optical imaging applications has been limited. A theory of intensity diffraction tomography (I-DT) has been proposed to circumvent this phase retrieval problem. In this work, we review the features of I-DT reconstruction theory that are relevant to optical microscopy. Computer-simulation studies are conducted to investigate the performance of reconstruction algorithms for a proposed I-DT microscope. The effects of data noise are assessed, and statistically optimal reconstruction strategies that employ multiple detector planes are proposed.

关键词： diffraction tomography three-dimensional microscopy tomographic image reconstruction

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Multi-wavelength phase imaging interference microscopy

Multi-wavelength phase imaging interference microscopy

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xiii

作者： Warnasooriya, Nilanthi Kim, Myung K. Univ S Florida Dept Phys Tampa FL 33620 USA

ISBN: (纸本)0819461326

Multi-wavelength phase imaging interferography is a technique that combines phase-shifting interferometry with multiwavelength phase unwrapping. It can be used to obtain phase profile of an object without 2 pi ambiguities inherent to single wavelength phase images. In this technique, a Michelson-type interferometer is illuminated by an LED and the reference mirror is dithered for obtaining interference images at four phase quadratures, which are combined to calculate the phase of the object surface. The 2 pi discontinuities are removed by repeating the experiment using two or three LEDs at different wavelengths, which yields phase images of effective wavelength much longer than the original. The resulting image is a profile of the object surface with a height range of several microns and noise levels of 10's of nm. The technique is applied to imaging of phase profile of microscopic objects. The interferographic images using broadband source are significantly less affected by coherent noise, and there are a number of other advantages, such as lower cost and ease of operation.

关键词： interference microscopy phase shift phase measurement

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