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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV"

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three-dimensional and multidimensional microscopy: image acquisition and processing XXXI

Three-Dimensional and Multidimensional Microscopy: Image Acq...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXXI 2024

ISBN: (纸本)9781510669550

The proceedings contain 14 papers. The topics discuss include: light-field microscopy to study cardiac arrhythmias in zebrafish larvae;on-the-fly deconvolution of diagonally-scanned airy lightsheet microscopy;image projection through a multimode fiber with reference-lessly retrieved transmission matrix;design and fabrication of universal resolution targets for microsphere-assisted microscopy;normalized level set model for segmentation of low-contrast objects in 2- and 3- dimensional images;development of long axial range 3D single-particle tracking by PSF engineering using birefringent substrates;Chernoff information for creating maximally distinctive engineered point spread functions for multi-color single molecule imaging;and hyperspectral microscopy as a particle exposure assessment tool.

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three-dimensional and multidimensional microscopy: image acquisition and processing XXX

Three-Dimensional and Multidimensional Microscopy: Image Acq...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXX 2023

ISBN: (纸本)9781510658752

The proceedings contain 13 papers. The topics discussed include: compressive time-resolved multispectral fluorescence microscopy with single-pixel camera;optical sectioning microscopy with structured illumination and single-pixel detection;high-speed light-sheet microscopy imaging and data post-processing using custom hardware and software solutions;utilizing fluorescent beads to mimic single fluorophores;single-shot capture bright-field and digital holographic microscopy for the analysis of living cells;simulations of imperfect refractive index matching in scanning laser optical tomography and a method for correction;super-resolution in confocal microscopy using generative adversarial networks with paired and unpaired data;estimation of 3D point spread function of a widefield microscope by stochastic least square minimization method;and design and implementation of plenoptic imaging system with reduced aberrations.

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three-dimensional and multidimensional microscopy: image acquisition and processing XXIX

Three-Dimensional and Multidimensional Microscopy: Image Acq...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXIX 2022

ISBN: (纸本)9781510648036

The proceedings contain 12 papers. The topics discussed include: high-resolution volumetric imaging using hybrid image of Fourier light-field microscopy and wide-field microscopy;quantification of collagen networks in mammary tumors using TPEF and laser-based tomography;low dosage 3D volume fluorescence microscopy imaging using compressive sensing;multispectral time-resolved fluorescence microscopy based on compressive acquisitions;a compact open-top light-sheet microscope for optofluidic imaging;validation of excitation-scan hyperspectral multi-faceted mirror array prototype system advancements to hyperspectral imaging applications;efficient and versatile aberration correction through sensorless adaptive optics;and hyperspectral imaging and adaptive thresholding to identify agonist-induced camp signals in pulmonary microvascular endothelial cells.

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three-dimensional and multidimensional microscopy: image acquisition and processing XXVIII

Three-Dimensional and Multidimensional Microscopy: Image Acq...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXVIII 2021

ISBN: (纸本)9781510641334

The proceedings contain 15 papers. The topics discussed include: sparsity-based approach for 3D super-resolution microscopy from correlation information of high emitter-density frames;quantitative phase imaging-based machine learning approaches for the analysis of adherent and suspended cells;automatic nuclei segmentation based on fuzzy C- mean;evaluation of different illumination designs for tunable 3D structured illumination microscopy through model-based restoration;high-throughput 3D imaging with compressive lens-free tomography;intensity uniformity optimization in spatial-light-modulator-based multifocal microscope;and method for measurement of collagen monomer orientation in fluorescence microscopy.

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Fast volumetric seizure model imaging in zebrafish brain with light sheet microscopy 31

Fast volumetric seizure model imaging in zebrafish brain wit...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXXI 2024

作者： Liu, Bingxi Liu, Yang Duffy, Carly Lauderdale, James D. Kner, Peter School of Electrical and Computer Engineering University of Georgia AthensGA30602 United States Department of Cellular Biology University of Georgia AthensGA30602 United States

ISBN: (纸本)9781510669550

Light Sheet Fluorescence microscopy (LSFM) is an ideal tool for imaging model organisms that are hundreds of microns in size, providing high resolution and fast frame rates. In order to extend LSFM to rapid 3D volumetric imaging, a popular method is to add an electrically tunable lens (ETL) in the detection path of the LSFM. But for larger fields of view and higher NA objectives, the ETL introduces aberrations in the system. Here, we developed an LSFM with adaptive optics and an ETL for rapid focusing. We demonstrate that the system enables imaging over a volume of 499 × 499 × 140 μm3 with a volumetric speed of 4 Hz. We apply the system to image neural activity in the zebrafish larvae to capture rare seizure events. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

关键词： Fluorescence microscopy

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three-dimensional multi-directional light-sheet microscopy with structured illumination 31

Three-dimensional multi-directional light-sheet microscopy w...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXXI 2024

作者： Song, Ming Liu, Yang Wu, Hsueh-Fu Zeltner, Nadja Kner, Peter A. School of Electrical and Computer Engineering University of Georgia AthensGA United States Chan Zuckerberg Biohub San FranciscoCA United States Center for Molecular Medicine University of Georgia AthensGA United States Department of Biochemistry and Molecular Biology University of Georgia AthensGA United States Department of Cellular Biology University of Georgia AthensGA United States

ISBN: (纸本)9781510669550

Light-Sheet Fluorescence microscopy (LSFM) has demonstrated its effectiveness in imaging many model organisms. However, traditional LSFMs often exhibit low resolution and encounter striping artifacts. Here, we introduce a novel LSFM design to address these challenges of imaging large biological samples. Our system employs a single-objective light-sheet geometry, incorporating Structured Illumination microscopy (SIM) to allow multi-direction light-sheet illumination. This combination aims to rectify striping artifacts and achieve improved lateral resolutions of better than 200 nm over a 277 × 277 × 500 μm3 volume. Our system is demonstrated through imaging beads, C. elegans and cerebellum organoids. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

关键词： Fluorescence microscopy

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Imaging the brain in vivo with reflectance phase-contrast confocal microscopy at 1650nm 31

Imaging the brain in vivo with reflectance phase-contrast co...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXXI 2024

作者： Delafontaine Martel, P. Zhang, Cong Linninger, Andreas A. Lesage, Frédéric Biomedical Engineering Institute Polytechnique Montreal Montreal Canada Montreal Heart Institute Montreal Canada Departement of Biomedical Engineering and Neurosurgery University of Illinois ChicagoIL United States

ISBN: (纸本)9781510669550

Recent advancements in sources and detectors operating in the NIR-II wavelengths have driven the emergence of NIR-II intrinsic microscopy. These significant technological strides were necessary because longer wavelengths are known to experience reduced scattering and absorption in biological tissue. Leveraging this optical advantage, the application of the NIR-II spectral domain in microscopy holds the potential to improve the depth of imaging and preserve coherence depth. In this study, we showcase the integration of phase-contrast imaging into a NIR-II reflectance confocal microscope for cortical imaging. By capturing images of cortical cell bodies at depths of up to 800 μm, we demonstrate that the implementation of phase contrast provides clear delineation of cortical cell edges, including myelinated axons, blood vessels, and cortical cell bodies. Additionally, we devised a computational method to enhance dynamic components and generate a digitized vascular network from the acquired images. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

关键词： Reflection

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Optical sectioning microscopy with structured illumination and single-pixel detection 30

Optical sectioning microscopy with structured illumination a...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXX 2023

作者： Ordóñez, Luis Ipus, Erick Lenz, Armin J.M. Lancis, Jesús Tajahuerce, Enrique Universitat Jaume I Castelló12071 Spain

ISBN: (纸本)9781510658752

We propose a new single pixel microscope with optical sectioning properties by using structured illumination techniques. The method uses single-pixel imaging (SPI) techniques by interrogating the sample with a series of spatially resolved patterns and measuring the output intensities with a non-spatial resolution detector. Moreover, optical sectioning is obtained by adding a grating to the system and employing structured illumination microscopy (SIM). The system allows us to perform 3D bright-field and fluorescence microscopy. We apply compressive sensing techniques to decrease the acquisition time. © 2023 SPIE. All rights reserved.

关键词： Fluorescence microscopy

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image projection through a multimode fiber with reference-lessly retrieved transmission matrix 31

Image projection through a multimode fiber with reference-le...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXXI 2024

作者： Zhong, Jingshan Wen, Zhong Li, Quanzhi Deng, Qilin Yang, Qing Research Center for Humanoid Sensing Zhejiang Lab Hangzhou311100 China State Key Laboratory of Extreme Photonics and Instrumentation College of Optical Science and Engineering International Research Center for Advanced Photonics Zhejiang University Hangzhou310027 China

ISBN: (纸本)9781510669550

image projection through a multimode fiber (MMF) or scattering media has applications ranging from optogenetics to near eye-displays. It requires developing computer-generated holography algorithm to obtain phase pattern of spatial light modulator. In order to accurately project light, conventional methods measured the transmission matrix (TM) of the imaging system by interference. However, it is sensitive to phase instability, easily caused by thermal drift and mechanical vibration. In this work, we proposed to use the TM retrieved from intensity-only measurements and develop a nonlinear optimization algorithm to obtain the displayed phase patterns. Our method formulates the forward model with the retrieved TM, derives the analytical derivative and adopts a second-order optimization method. We validate the improved quality of the projected intensity image by an experiment setup with a MMF. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

关键词： Multimode fibers

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Multispectral digital holographic microscopy with tunable supercontinuum lasers 31

Multispectral digital holographic microscopy with tunable su...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXXI 2024

作者： Kurtz, Tobias Barroso, Álvaro Picazo-Bueno, José Ángel Ketelhut, Steffi Schnekenburger, Jürgen Kemper, Björn Biomedical Technology Center of the Medical Faculty University of Muenster MuensterD-48149 Germany Department Department of Optics Optometry and Vision Science Faculty of Physics University of Valencia Burjassot46100 Spain

ISBN: (纸本)9781510669550

Supercontinuum (SC) lasers in combination with tunable acousto-optic filters facilitate multiwavelength digital holographic microscopy (DHM) with a broad spectral range. However, to achieve optimized quantitative phase imaging (QPI) in off-axis DHM the coherence properties of the tunable light source and the holographic carrier interference fringe pattern formation have to be considered as they are wavelength dependent. We thus determined experimentally the wavelength dependency of coherence length and off-axis carrier fringe frequency in a Michelson interferometer-based multi-spectral DHM setup with a continuously tunable SC laser and moreover analyzed the impact of both parameters on hologram formation and QPI image quality. The results are found in good agreement with theoretical predictions and provide essential information for a reliable adjustment of SC laser based multispectral off-axis DHM systems. Finally, the application of multispectral-DHM for reduction of coherence induced disturbances in quantitative phase images of living pancreatic tumor cells in illustrated. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

关键词： Michelson interferometers

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