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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX"

共 592 条记录，以下是101-110 订阅

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Two-dimensional compressive sensing in spectral domain optical coherence tomography 22

Two-dimensional compressive sensing in spectral domain optic...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXII

作者： Xu, Daguang Huang, Yong Kang, Jin U. Johns Hopkins Univ Dept Elect & Comp Engn Baltimore MD 21218 USA

ISBN: (纸本)9781628414202

In this paper, we proposed a novel compressive sensing (CS) method in spectral domain optical coherence tomography (SD OCT), which reconstructs B-scan image using a subset of the spectral data that is under-sampled in both axial and lateral dimensions. Thus a fraction of the A-scans for a B-scan are acquired;the spectral data of each acquired A-scan is under-sampled. Compared with the previous studies, our method further reduces the overall size of the spectral measurements. Experimental results show that our approach can obtain high quality B-scan image using 25% spectral data, which takes 50% number of A-scans and acquires 50% spectral data for each selected A-scan.

关键词： Compressive sensing optical coherence tomography reconstruction algorithm image processing

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three dimensional imaging using phase retrieval with two focus planes 13

Three dimensional imaging using phase retrieval with two foc...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Ilovitsh, Tali Ilovitsh, Asaf Weiss, Aryeh Meir, Rinat Zalevsky, Zeev Bar Ilan Univ Fac Engn IL-5290002 Ramat Gan Israel Bar Ilan Univ Bar Ilan Inst Nanotechnol & Adv Mat IL-5290002 Ramat Gan Israel

ISBN: (纸本)9781628419474

This work presents a technique for a full 3D imaging of biological samples tagged with gold-nanoparticles (GNPs) using only two images, rather than many images per volume as is currently needed for 3D optical sectioning microscopy. The proposed approach is based on the Gerchberg-Saxton (GS) phase retrieval algorithm. The reconstructed field is free space propagated to all other focus planes using post processing, and the 2D z-stack is merged to create a 3D image of the sample with high fidelity. Because we propose to apply the phase retrieving on nano particles, the regular ambiguities typical to the Gerchberg-Saxton algorithm, are eliminated. In addition, since the method requires the capturing of two images only, it can be suitable for 3D live cell imaging. The proposed concept is presented and validated both on simulated data as well as experimentally.

关键词： three-dimensional microscopy Cellular imaging gold nanoparticle Phase retrieval

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Spectral-domain interferometry for quantitative DIC microscopy

Spectral-domain interferometry for quantitative DIC microsco...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Li, Chengshuai Zhu, Yizheng Virginia Tech Bradley Dept Elect & Comp Engn Blacksburg VA 24060 USA

ISBN: (纸本)9780819498625

A spectral-domain differential interference contrast (SD-DIC) microscopy system is presented for quantitative imaging of both reflective and transparent samples. The spectral-domain interferometry, combined with the common-path DIC geometry, provides a shot noise-limited sensitivity of 14.3pm in optical pathlength gradient measurement. The optical resolution of the system was characterized using images of a USAF resolution target. Fused silica microspheres were imaged to demonstrate the reconstruction of two-dimensional optical pathlength topography from measured gradient fields. The exquisite sensitivity of the system showed potential in quantitative imaging of sub-diffraction limit objects such as gold nanoparticles.

关键词： Differential interference contrast quantitative phase imaging spectral-domain interferometry

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Investigation of 3D microscopy using intensity diffraction tomography

Investigation of 3D microscopy using intensity diffraction t...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XIII

作者： Anastasio, Mark A. Huang, Yin Gbur, Greg Carney, P. Scott IIT Dept Biomed Engn Chicago IL 60616 USA Univ North Carolina Charlotte Dept Phys & Opt Sci Charlotte NC USA Univ Illinois Dept Elect & Comp Engn Urbana IL USA

ISBN: (纸本)0819461326

Diffraction tomography (DT) is an established imaging technique for reconstructing the complex-valued refractive index distribution of a weakly scattering 3D sample. Due to experimental difficulties associated with the direct measurement of the phase of an optical wavefield, the effectiveness of DT for optical imaging applications has been limited. A theory of intensity diffraction tomography (I-DT) has been proposed to circumvent this phase retrieval problem. In this work, we review the features of I-DT reconstruction theory that are relevant to optical microscopy. Computer-simulation studies are conducted to investigate the performance of reconstruction algorithms for a proposed I-DT microscope. The effects of data noise are assessed, and statistically optimal reconstruction strategies that employ multiple detector planes are proposed.

关键词： diffraction tomography three-dimensional microscopy tomographic image reconstruction

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three-dimensional image of sugar content visualization in a melon by spectral information

Three-dimensional image of sugar content visualization in a ...

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Conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Sugiyama, J Ogawa, Y Natl Food Res Inst Tsukuba Ibaraki 3058642 Japan

ISBN: (纸本)081943535X

In order to visualize sugar content of a melon, the relationship between sugar content and absorption spectra was investigated using a near-infrared (NIR) spectrometer. The absorbance at 676nm, which is close to the chlorophyll absorption band, had a high inverse correlation with sugar content. A high-resolution cooled CCD imaging camera with the band-pass filter of 676nm was used to capture the spectral absorption image. The calibration method for converting the absorbance on the image into Brix sugar content was developed in accordance with MR techniques. Applying this method to each pixel of the absorption image, a color distribution map of the sugar content was constructed. In addition, a special slicing device that can cut a melon in each 5mm thickness was developed in order to create a 3-D image of sugar content distribution.

关键词： three-dimensional near infrared spectral image sugar content melon

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Pupil Polarimetry using Stress-Engineered Optical Elements

Pupil Polarimetry using Stress-Engineered Optical Elements

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Beckley, Amber M. Brown, Thomas G. Univ Rochester Inst Opt Rochester NY 14627 USA

ISBN: (纸本)9780819479662

Stress-engineered optical elements show fascinating and potentially useful effects when placed at the pupil plane of an imaging system. When illuminated by a beam of spatially uniform polarization, a snapshot (single measurement) polarimetry method can be constructed. We expand upon this method to perform snapshot pupil polarimetry for spatially varying pupil polarization. We present the theory for snapshot non-uniform pupil polarization measurement using a stress-engineered optical element, as well as simulation results.

关键词： Polarimetry microscopy Cylindrical Vector Beams

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High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera

High speed slit-scanning confocal laser microscopy with an a...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Han, SM Im, KB Park, H Kim, D Kim, BM Yonsei Univ Dept Biomed Engn Kangwon Do 220710 South Korea

ISBN: (纸本)0819456756

Using an acousto-optic beam deflector and a line scan camera, we constructed a non-mechanical, slit-scanning confocal microscope. It generates two-dimensional 512x512 images with speed of 60 frames/sec, which can be easily expanded, to 100 frames/sec. The measured axial and lateral resolutions of the system are 3.3 mu m and < 1 mu m, respectively, with a 50X objective. This simple design can produce frames rates faster than other commercial products with comparable resolution, which may be useful for analysis of rapid interactions in various biological applications.

关键词： confocal microscope three-dimensional microscopy acousto-optic deflector line scan CCD

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A comparison of methods for optical sectioning using structured illumination microscopy

A comparison of methods for optical sectioning using structu...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Thomas, Benjamin Momany, Michelle Kner, Peter Univ Georgia Coll Engn Athens GA 30602 USA Univ Georgia Dept Plant Biol Athens GA 30602 USA

ISBN: (纸本)9780819493583

Structured Illumination microscopy is a simple and effective method to remove out-of-focus light in widefield fluorescence microscopy. Neil et al. originally proposed a simple square-law method for calculating the optically sectioned image from the three raw images with the structured illumination pattern super-imposed. However, the Neil method does not make the most efficient use of the three raw images. The three structured illumination images can also be used to separate three copies of the image covering shifted regions of frequency space in a similar manner to that developed by Gustafsson et al. These can then be combined using a generalized Wiener filter to create an image with a well-behaved optical transfer function in which the missing cone has been filled in, providing optical sectioning. Here, we compare the Neil and Gustafsson methods and show that the Gustafsson method provides an image with higher fidelity and a better Signal to Noise Ratio (SNR) at low photon counts. We apply the two methods to images of fluorescent beads and GFP labeled septins in Aspergillus nidulans.

关键词： fluorescence microscopy structured illumination microscopy three-dimensional imaging bioimaging

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Nano-scale optical imaging technique

Nano-scale optical imaging technique

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Wu, S Tang, YC PEER Ctr Covina CA 91722 USA

ISBN: (纸本)0819452327

The optical resolution limits the observation of fine features with size below diffraction limit, e.g. 500nm. We propose and demonstrate a technique that uses collimated laser scattering to characterize such features. We use a new illumination technique that let us image bio-molecules down to 50nm with optical microscopy. We observed vesicles with size down to 50nm and visualize the flow of such tiny molecules in the live cells. We could also characterize the detailed size and molecular weight of such particles.

关键词： single particle laser scattering microscopy

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A compact polarization converter to observe molecular orientation

A compact polarization converter to observe molecular orient...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Hashimoto, Mamoru Yoshiki, Keisuke Kanamaru, Ryosuke Hashimoto, Nobuyuki Araki, Tsutomu Osaka Univ Grad Sch Engn Sci 1-3 Machikaneyama Toyonaka Osaka 5608531 Japan Citizen Watch Co Ltd MHT R&D Dept Tokorozawa Saitama 3598511 Japan

ISBN: (纸本)9780819465566

We developed a compact polarization-converter using two liquid-crystal spatial-light-modulators with eight electrodes. The converter converted a linearly polarized beam to two orthogonal linearly polarized beams and a radially polarized beam, and the direction of the electric filed at the focal point were controlled three-dimensionally. We constructed a second-harmonic-generation microscope using the polarization-converter to observe three-dimensional molecular orientation and demonstrated the detectability of molecular orientation.

关键词： molecular orientation nonlinear microscopy second-harmonics-generation pupil filtering radially polarized beam

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