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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XIII

作者： Ventalon, Cathie Mertz, Jerome Boston Univ Dept Biomed Engn 44 Cummington St Boston MA 02215 USA

ISBN: (纸本)0819461326

We present a new fluorescence microscopy technique that provides depth discrimination in thick tissue. The technique relies on a simple modification to a conventional wide-field microscope, and consists in illuminating the sample with a sequence of speckle patterns and displaying the RMS or the variance of the resultant sequence of fluorescent images. We demonstrate quasi-confocal optical sectioning with an axial resolution of 5 microns FWHM. The lateral resolution is identical to the widefield microscope, namely 0.6 micron FWHM. images of a mouse brain are compared with standard wide-field images, demonstrating an efficient quasi-sectioning capacity in thick tissue throughout approximatively 100 microns depth. This is achieved because of the high contrast maintained by speckle in a scattering media.

关键词： fluorescence microscopy optical sectioning 3D imaging linear microscopy confocal microscopy speckle structured light illumination thick tissue imaging

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System for fluorescence lifetime imaging microscopy

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Proceedings of SPIE - The International Society for Optical Engineering 1999年 3605卷 55-64页

作者： van Geest, Lambertus K. Boddeke, Frank R. van Dijk, Pieter W. Kamp, Arjen F. van der Oord, Cornelis J.R. Stoop, Karel W.J. Lambert Instruments Netherlands

ISBN: (纸本)0819430757;9780819430755

An affordable, robust and easy-to-use fluorescence lifetime imaging microscopy (FLIM) workstation that is completely automated and does not need any difficult calibration procedure is introduced. The workstation consists of a standard fluorescence microscope, a modulated excitation light source, a camera, a modulation signal generator and acquisition/processing software. An evaluation of the system and its critical components is presented.

关键词： Optical microscopy

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Pupil Polarimetry using Stress-Engineered Optical Elements

Pupil Polarimetry using Stress-Engineered Optical Elements

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Beckley, Amber M. Brown, Thomas G. Univ Rochester Inst Opt Rochester NY 14627 USA

ISBN: (纸本)9780819479662

Stress-engineered optical elements show fascinating and potentially useful effects when placed at the pupil plane of an imaging system. When illuminated by a beam of spatially uniform polarization, a snapshot (single measurement) polarimetry method can be constructed. We expand upon this method to perform snapshot pupil polarimetry for spatially varying pupil polarization. We present the theory for snapshot non-uniform pupil polarization measurement using a stress-engineered optical element, as well as simulation results.

关键词： Polarimetry microscopy Cylindrical Vector Beams

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Real-time optically sectioned wide-field microscopy employing structured light illumination and a CMOS detector

Real-time optically sectioned wide-field microscopy employin...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Mitic, J Anhut, T Serov, A Lasser, T Bourquin, S Swiss Fed Inst Technol Inst Imaging & Appl Opt CH-1015 Lausanne Switzerland

ISBN: (纸本)0819447641

Real-time optically sectioned microscopy is demonstrated using an AC-sensitive detection concept realized with smart CMOS image sensor and structured light illumination by a continuously moving periodic pattern. We describe two different detection systems based on CMOS image sensors for the detection and on-chip processing of the sectioned images in real time. A region-of-interest is sampled at high frame rate. The demodulated signal delivered by the detector corresponds to the depth discriminated image of the sample. The measured FWHM of the axial response de ends on the spatial frequency of the projected grid illumination and is in the pm-range. The effect of using broadband incoherent illumination is discussed. The performance of these systems is demonstrated by imaging technical as well as biological samples.

关键词： optically sectioned microscopy structured illumination CMOS image sensor

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Point spread function models for digital holographic microscopy

Point spread function models for digital holographic microsc...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Marian, A Depeursinge, C Swiss Fed Inst Technol Inst Biomed Imaging Opt & Engn CH-1015 Lausanne Switzerland

ISBN: (纸本)0819452327

Few models, based on the diffraction theory, are proposed in order to evaluate the point spread function of different microscope objectives used in a digital holographic microscope. Because in holography the phase information is essential, a 3D amplitude point spread function (APSF), modulus and phase, is necessary, in order to properly deconvolute the 3D images obtained. Scalar Debye theory, paraxial approximation and vectorial Debye theory are used to solve the diffraction problem and the theoretical predicted 3D APSFs obtained. with these models are compared.

关键词： point spread function diffraction digital holographic microscopy

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three dimensional imaging using phase retrieval with two focus planes 13

Three dimensional imaging using phase retrieval with two foc...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Ilovitsh, Tali Ilovitsh, Asaf Weiss, Aryeh Meir, Rinat Zalevsky, Zeev Bar Ilan Univ Fac Engn IL-5290002 Ramat Gan Israel Bar Ilan Univ Bar Ilan Inst Nanotechnol & Adv Mat IL-5290002 Ramat Gan Israel

ISBN: (纸本)9781628419474

This work presents a technique for a full 3D imaging of biological samples tagged with gold-nanoparticles (GNPs) using only two images, rather than many images per volume as is currently needed for 3D optical sectioning microscopy. The proposed approach is based on the Gerchberg-Saxton (GS) phase retrieval algorithm. The reconstructed field is free space propagated to all other focus planes using post processing, and the 2D z-stack is merged to create a 3D image of the sample with high fidelity. Because we propose to apply the phase retrieving on nano particles, the regular ambiguities typical to the Gerchberg-Saxton algorithm, are eliminated. In addition, since the method requires the capturing of two images only, it can be suitable for 3D live cell imaging. The proposed concept is presented and validated both on simulated data as well as experimentally.

关键词： three-dimensional microscopy Cellular imaging gold nanoparticle Phase retrieval

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Gabor wavelet transform for dynamic analysis in digital holographic microscopy

Gabor wavelet transform for dynamic analysis in digital holo...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Zhong, Jingang Weng, Jiawen Hu, Cuiying Jinan Univ Inst Optoelect Engn Guangzhou 510632 Guangdong Peoples R China

ISBN: (纸本)9780819479662

Real time dynamic analysis of micro-object is one of the significant advantages of digital holographic microscopy, but the process of the spatial filtering limits the application of dynamic and automatic analysis in digital holographic microscopy. In this paper, a numerical reconstruction technique by means of Gabor wavelet transform (GWT) is presented for real time dynamic analysis. Appling the GWT to the hologram, the quantitative phase information of the micro-object is obtained automatically without the process of the spatial filtering. An experiment with a sequence of holograms of an animalcule is proposed for the dynamic and automatic analysis by employing the GWT method.

关键词： Digital holographic microscopy dynamic analysis wavelet transform full-field quantitative phase imaging

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Two-dimensional compressive sensing in spectral domain optical coherence tomography 22

Two-dimensional compressive sensing in spectral domain optic...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXII

作者： Xu, Daguang Huang, Yong Kang, Jin U. Johns Hopkins Univ Dept Elect & Comp Engn Baltimore MD 21218 USA

ISBN: (纸本)9781628414202

In this paper, we proposed a novel compressive sensing (CS) method in spectral domain optical coherence tomography (SD OCT), which reconstructs B-scan image using a subset of the spectral data that is under-sampled in both axial and lateral dimensions. Thus a fraction of the A-scans for a B-scan are acquired;the spectral data of each acquired A-scan is under-sampled. Compared with the previous studies, our method further reduces the overall size of the spectral measurements. Experimental results show that our approach can obtain high quality B-scan image using 25% spectral data, which takes 50% number of A-scans and acquires 50% spectral data for each selected A-scan.

关键词： Compressive sensing optical coherence tomography reconstruction algorithm image processing

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Fuzzy Logic Components for Iterative Deconvolution Systems

Fuzzy Logic Components for Iterative Deconvolution Systems

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Northan, Brian M. True North Image Proc Guilderland NY 12084 USA

ISBN: (纸本)9780819493583

Deconvolution systems rely heavily on expert knowledge and would benefit from approaches that capture this expert knowledge. Fuzzy logic is an approach that is used to capture expert knowledge rules and produce outputs that range in degree. This paper describes a fuzzy-deconvolution-system that integrates traditional Richardson-Lucy deconvolution with fuzzy components. The system is intended for restoration of 3D widefield images taken under conditions of refractive index mismatch. The system uses a fuzzy rule set for calculating sample refractive index, a fuzzy median filter for inter-iteration noise reduction, and a fuzzy rule set for stopping criteria.

关键词： Deconvolution Fuzzy Logic Spherical Aberration

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Investigation of 3D microscopy using intensity diffraction tomography

Investigation of 3D microscopy using intensity diffraction t...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XIII

作者： Anastasio, Mark A. Huang, Yin Gbur, Greg Carney, P. Scott IIT Dept Biomed Engn Chicago IL 60616 USA Univ North Carolina Charlotte Dept Phys & Opt Sci Charlotte NC USA Univ Illinois Dept Elect & Comp Engn Urbana IL USA

ISBN: (纸本)0819461326

Diffraction tomography (DT) is an established imaging technique for reconstructing the complex-valued refractive index distribution of a weakly scattering 3D sample. Due to experimental difficulties associated with the direct measurement of the phase of an optical wavefield, the effectiveness of DT for optical imaging applications has been limited. A theory of intensity diffraction tomography (I-DT) has been proposed to circumvent this phase retrieval problem. In this work, we review the features of I-DT reconstruction theory that are relevant to optical microscopy. Computer-simulation studies are conducted to investigate the performance of reconstruction algorithms for a proposed I-DT microscope. The effects of data noise are assessed, and statistically optimal reconstruction strategies that employ multiple detector planes are proposed.

关键词： diffraction tomography three-dimensional microscopy tomographic image reconstruction

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