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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX"

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Portable advanced off-axis camera for quantitative phase microscopy

Portable advanced off-axis camera for quantitative phase mic...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Monemhaghdoust, Z. Depeursinge, C. Moser, C. Ecole Polytech Fed Lausanne Lab Appl Photon Devices CH-1015 Lausanne Switzerland Lyncee Tec SA CH-1015 Lausanne Switzerland

ISBN: (纸本)9780819498625

We propose and experimentally demonstrate a device in which common-path interferometry combined with off-axis holographic geometry is used to realize a digital holographic camera which can be attached to the camera port of a conventional transmission microscope for complex wavefront analysis. A thick transmission volume grating recorded holographically into thick photosensitive glass splits the beam containing the sample information in two beams. The untouched transmitted beam creates the sample arm of the interferometer. The Bragg diffracted order of the grating is spectrally and spatially filtered by diffraction to generate a clean reference beam. Double passing the diffracted order through the grating using a retroreflector device provides filtering in two dimensions. The spatial filtering done by the grating which works based on high angular selectivity of thick volume gratings, reduces the alignment spatial sensitivity which is an advantage over the conventional spatial filtering done by pinholes. Besides, using a second thick grating, we introduce a desired coherence plane tilt in the reference beam which is sufficient to create high-visibility interference over the entire field of view. The full-field off-axis interferograms are created from which the amplitude and phase can be reconstructed. The advantage of the proposed camera is the insensitivity to the alignment, thus can be the basis for a standalone camera mountable on a standard optical microscope.

关键词： Diffraction gratings Digital holographic microscopy Portable camera Off-axis full-field imaging Phase microscopy

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New Light Field Camera based on Physical Based Rendering Tracing

New Light Field Camera based on Physical Based Rendering Tra...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Chung, Ming-Han Chang, Shan-Ching Lee, Chih-Kung Natl Taiwan Univ Inst Appl Mech Taipei Taiwan

ISBN: (纸本)9780819498625

Even though light field technology was first invented more than 50 years ago, it did not gain popularity due to the limitation imposed by the computation technology. With the rapid advancement of computer technology over the last decade, the limitation has been uplifted and the light field technology quickly returns to the spotlight of the research stage. In this paper, PBRT (Physical Based Rendering Tracing) was introduced to overcome the limitation of using traditional optical simulation approach to study the light field camera technology. More specifically, traditional optical simulation approach can only present light energy distribution but typically lack the capability to present the pictures in realistic scenes. By using PBRT, which was developed to create virtual scenes, 4D light field information was obtained to conduct initial data analysis and calculation. This PBRT approach was also used to explore the light field data calculation potential in creating realistic photos. Furthermore, we integrated the optical experimental measurement results with PBRT in order to place the real measurement results into the virtually created scenes. In other words, our approach provided us with a way to establish a link of virtual scene with the real measurement results. Several images developed based on the above-mentioned approaches were analyzed and discussed to verify the pros and cons of the newly developed PBRT based light field camera technology. It will be shown that this newly developed light field camera approach can circumvent the loss of spatial resolution associated with adopting a micro-lens array in front of the image sensors. Detailed operational constraint, performance metrics, computation resources needed, etc. associated with this newly developed light field camera technique were presented in detail.

关键词： Physical Based Rendering Tracing PBRT Light field Ray tracing image processing

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Implementation of PSF Engineering in High-Resolution 3D microscopy Imaging with a LCoS (Reflective) SLM

Implementation of PSF Engineering in High-Resolution 3D Micr...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： King, Sharon V. Doblas, Ana Patwary, Nurmohammed Saavedra, Genaro Mart-Nez-Corral, Manuel Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA Univ Valencia Dept Opt 3D Imaging & Display Lab E-46100 Burjassot Spain

ISBN: (纸本)9780819498625

Wavefront coding techniques are currently used to engineer unique point spread functions (PSFs) that enhance existing microscope modalities or create new ones. Previous work in this field demonstrated that simulated intensity PSFs encoded with a generalized cubic phase mask (GCPM) are invariant to spherical aberration or misfocus;dependent on parameter selection. Additional work demonstrated that simulated PSFs encoded with a squared cubic phase mask (SQUBIC) produce a depth invariant focal spot for application in confocal scanning microscopy. Implementation of PSF engineering theory with a liquid crystal on silicon (LCoS) spatial light modulator (SLM) enables validation of WFC phase mask designs and parameters by manipulating optical wavefront properties with a programmable diffractive element. To validate and investigate parameters of the GCPM and SQUBIC WFC masks, we implemented PSF engineering in an upright microscope modified with a dual camera port and a LCoS SLM. We present measured WFC PSFs and compare them to simulated PSFs through analysis of their effect on the microscope imaging system properties. Experimentally acquired PSFs show the same intensity distribution as simulation for the GCPM phase mask, the SQUBIC-mask and the well-known and characterized cubic-phase mask (CPM), first applied to high NA microscopy by Arnison et al.(10), for extending depth of field. These measurements provide experimental validation of new WFC masks and demonstrate the use of the LCoS SLM as a WFC design tool. Although efficiency improvements are needed, this application of LCoS technology renders the microscope capable of switching among multiple WFC modes.

关键词： microscopy wave-front encoding spatial light modulators liquid crystal

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The Gray Institute Open Microscopes applied to radiobiology and protein interaction studiesZ

The Gray Institute Open Microscopes applied to radiobiology ...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Barber, P. R. Tullis, I. D. C. Rowley, M. I. Martins, C. D. Weitsman, G. Lawler, K. Coffey, M. Woodman, N. Gillett, C. E. Ng, T. Vojnovic, B. Univ Oxford Gray Inst Radiat Oncol & Biol Dept Oncol Oxford OX1 2JD England Inst Math & Molecular Biomed Kings Coll London London England Kings Coll London Div Cancer Studies London England Kings Coll London Randall Div London England Kings Coll London Breakthrough Breast Cancer Res Unit London England Kings Coll London Guys & St Thomas Breast Tissue & Data Bank London England

ISBN: (纸本)9780819498625

We describe an 'open' design methodology for wide-field fluorescence, confocal and fluorescence lifetime imaging microscopy (FLIM), and how the resulting microscopes are being applied to radiation biology and protein activity studies in cells and human tissue biopsies. The design approach allows easy expansion as it moves away from the use of a monolithic microscope body to small, commercial off-the-shelf and custom made modular components. Details have been made available under an open license for non-commercial use at http://***/similar to atdgroup. Two radiobiology 'end-stations' have been constructed which enable fast radiation targeting and imaging of biological material opening up completely novel studies, where the consequences of ionising radiation (signaling and protein recruitment) can be studied in situ, at short times following irradiation. One is located at Surrey University, UK, where radiation is a highly focused in beam (e.g. protons, helium or higher mass ions). The second is installed at the Gray Institute linear accelerator facility, Oxford University, which uses sub-microsecond pulses of 6 MeV electrons. FLIM capabilities have enhanced the study of protein-protein interactions in cells and tissues via Frster Resonance Energy Transfer (FRET). Extracting FRET signals from breast cancer tissue is challenging because of endogenous and fixation fluorescence;we are investigating novel techniques to measure this robustly. Information on specific protein interactions from large numbers of patient tumors will reveal prognostic and diagnostic information.

关键词： Tissues Fluorescence resonance energy transfer Microscopes image filtering Atrial fibrillation Fluorescence lifetime imaging Optical filters Proteins Luminescence Linear filtering

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three-dimensional and multidimensional microscopy: image acquisition and processing XX

Three-Dimensional and Multidimensional Microscopy: Image Acq...

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three-dimensional and multidimensional microscopy: image acquisition and processing XX

ISBN: (纸本)9780819493583

The proceedings contain 31 papers. The topics discussed include: a new expanded point information content design approach for 3D live-cell microscopy at video rates;Fuzzy logic components for iterative deconvolution systems;two approximations for the geometric model of signal amplification in an electron-multiplying charge-coupled device detector;intensity-based segmentation an visualization of cells in 3D microscopic images using the GPU;GPU-based image registration correlation microscopy, and reflection mode correlation microscopy;adapting phasar analysis for nonlinear pump-probe microscopy;comparison of computational methods developed to address depth-variant imaging in fluorescence microscopy;and assessment of robust reconstruction algorithms for compressive sensing spectral-domain optical coherence tomography.

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Digital holographic confocal microscope

Digital holographic confocal microscope

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Goy, Alexandre S. Psaltis, Demetri Ecole Polytech Fed Lausanne CH-1015 Lausanne Switzerland

ISBN: (纸本)9780819493583

We demonstrate experimentally a scanning confocal microscopy technique based on digital holographic recording of the scanned spot. The data collected in this way contains all the information to produce three-dimensional images. Several methods to treat the data are presented, such as the dynamic placement of the pinhole. Examples of reflection and transmission images of epithelial cells and mouse brain tissue are shown. The computations can be performed in real time, the speed being limited only by the frame rate of the camera. This method enables a convenient implementation of confocal microscopy, especially in transmission as no de-scan device is required.

关键词： digital holography confocal microscopy three-dimensional imaging

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Phase imaging with partially coherent light

Phase imaging with partially coherent light

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Waller, Laura Univ Calif Berkeley Dept Elect Engn & Comp Sci Berkeley CA 94702 USA

We review several quantitative phase imaging methods that retrieve phase from a through-focus series of intensity images (specifically, iterative methods and Transport of Intensity) and compare their performance when ... 详细信息

ISBN: (纸本)9780819493583

关键词： Phase imaging partial coherence microscopy transport of intensity

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GPU-based image Registration in Aperture Correlation microscopy, and Reflection Mode Correlation microscopy

GPU-based Image Registration in Aperture Correlation Microsc...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Fafchamps, Lionel J. Neil, Mark A. A. Juskaitis, Rimas Imperial Coll London London England Aurox Ltd Abingdon Oxon England

ISBN: (纸本)9780819493583

Aperture Correlation microscopy (ACM) is a fluorescence microscopy technique capable of depth resolved imaging and enhanced lateral resolution at real-time acquisition rates. It relies on the subtraction of 2 separate images from different cameras which must be registered to the sub-pixel level. In order to achieve real-time registration and subtraction, the graphics processing unit (GPU) is used to apply a transformation from one frame to the other, resulting in a system capable of processing over 200 frames per second on modest hardware (GeForce 330M). Currently, this rate is limited by camera acquision to 16fps. Additionally, a novel reflection mode correlation microscope is introduced which functions on similar principles as the fluorescent system but can be used to examine reflective samples. images and z-stacks taken with this system are presented here.

关键词： Cameras microscopy image registration Microscopes Confocal microscopy image processing Objectives Polarizers image transmission Reflectivity

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A comparison of methods for optical sectioning using structured illumination microscopy

A comparison of methods for optical sectioning using structu...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Thomas, Benjamin Momany, Michelle Kner, Peter Univ Georgia Coll Engn Athens GA 30602 USA Univ Georgia Dept Plant Biol Athens GA 30602 USA

ISBN: (纸本)9780819493583

Structured Illumination microscopy is a simple and effective method to remove out-of-focus light in widefield fluorescence microscopy. Neil et al. originally proposed a simple square-law method for calculating the optically sectioned image from the three raw images with the structured illumination pattern super-imposed. However, the Neil method does not make the most efficient use of the three raw images. The three structured illumination images can also be used to separate three copies of the image covering shifted regions of frequency space in a similar manner to that developed by Gustafsson et al. These can then be combined using a generalized Wiener filter to create an image with a well-behaved optical transfer function in which the missing cone has been filled in, providing optical sectioning. Here, we compare the Neil and Gustafsson methods and show that the Gustafsson method provides an image with higher fidelity and a better Signal to Noise Ratio (SNR) at low photon counts. We apply the two methods to images of fluorescent beads and GFP labeled septins in Aspergillus nidulans.

关键词： fluorescence microscopy structured illumination microscopy three-dimensional imaging bioimaging

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A new expanded point information content design approach for 3D live-cell microscopy at video rates

A new expanded point information content design approach for...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Zahreddine, Ramzi N. Cormack, Robert H. Cogswell, Carol J. Univ Colorado Dept Elect Comp & Energy Engn Boulder CO 80309 USA

ISBN: (纸本)9780819493583

Through a combination of optical design and algorithm development, a new expanded point information content ( EPIC) microscope has been developed that is capable of extending the depth of field while simultaneously super locating the depth position of complex biological objects to within an accuracy of 75 nm. The data is then combined to form 3D animations of live-cell biological specimens. This is accomplished without the need to acquire multi-focal image stacks and is thus well suited for high-speed imaging.

关键词： 4D microscopy PSF engineering extended depth of field quantitative depth ranging computational optics live-cell imaging fluorescence microscopy

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