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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xix

作者： Toy, M. Fatih Richard, Stephane Kuehn, Jonas Franco-Obregon, Alfredo Egli, Marcel Depeursinge, Christian Ecole Polytech Fed Lausanne Adv Photon Lab CH-1015 Lausanne Switzerland ETH Space Biol Grp CH-8005 Zurich Switzerland Ecole Polytech Fed Lausanne Biomol Screening Facil CH-1015 Lausanne Switzerland

ISBN: (纸本)9780819488701

Digital holographic microscopy (DHM) has been gaining interest from cell biology community because of its label free nature and quantitative phase signal output. Besides, fast shutter time, image reconstruction by numerical propagation of the wave fields, and numerical compensation of the aberrations are other intrinsic advantages of this technique that can be explored for harsh imaging conditions. In the frame of this work, a transmission type DHM is developed with a decoupled epifluorescence microscopy mode for cytomorphological monitoring under zero gravity and hyper gravity. With the implemented automatic post processing routines, real time observation of the cell morphology is proven to be feasible under the influence of mechanical disturbances of zero gravity platforms. Post processing of holograms is composed from dynamic numerical compensation of holograms, robust autofocusing and phase image registration. Experiments on live myoblast cells are carried out on two different platforms;random positioning machine (RPM), a ground base microgravity simulation platform, and parabolic flight campaign (PFC), a fixed wing plane flight providing short durations of alternating gravity conditions. Results show clear perinuclear phase increase. During seconds scale microgravity exposure, measurable scale morphological modifications are observed with the accumulated effect of repetitive exposures and short breaks.

关键词： microscopy holography digital holographic microscopy fluorescence microscopy microgravity cell morphology cytoskeleton

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Hyperspectral fluorescence microscopy based on compressed sensing

Hyperspectral fluorescence microscopy based on compressed se...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xix

作者： Studer, Vincent Bobin, Jerome Chahid, Makhlad Moussavi, Hamed Candes, Emmanuel Dahan, Maxime Univ Bordeaux Interdisciplinary Inst Neurosci UMR 5297 F-33000 Bordeaux France Univ Bordeaux Interdisciplinary Inst Neurosci UMR 5297 F-33000 Bordeaux France CEA Saclay IRFU SEDI Sap Grenoble France Stanford Univ Dept Math Stanford CA 94305 USA Stanford Univ Dept Stat & Elect Engn Stanford CA 94305 USA Univ Paris 06 Ecole Normale Super CNRS Lab Kastler BrosselUMR 8552 Paris France

ISBN: (纸本)9780819488701

In fluorescence microscopy, one can distinguish two kinds of imaging approaches, wide field and raster scan microscopy, differing by their excitation and detection scheme. In both imaging modalities the acquisition is independent of the information content of the image. Rather, the number of acquisitions N, is imposed by the Nyquist-Shannon theorem. However, in practice, many biological images are compressible (or, equivalently here, sparse), meaning that they depend on a number of degrees of freedom K that is smaller that their size N. Recently, the mathematical theory of compressed sensing (CS) has shown how the sensing modality could take advantage of the image sparsity to reconstruct images with no loss of information while largely reducing the number M of acquisition. Here we present a novel fluorescence microscope designed along the principles of CS. It uses a spatial light modulator (DMD) to create structured wide field excitation patterns and a sensitive point detector to measure the emitted fluorescence. On sparse fluorescent samples, we could achieve compression ratio N/M of up to 64, meaning that an image can be reconstructed with a number of measurements of only 1.5 % of its pixel number. Furthemore, we extend our CS acquisition scheme to an hyperspectral imaging system.

关键词： Fluorescence microscopy compressive sensing hyperspectral imaging

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Self-referenced quantitative phase microscopy

Self-referenced quantitative phase microscopy

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three-dimensional and multidimensional microscopy: image acquisition and processing xix

作者： Hillman, Timothy R. Lue, Niyom Sung, Yongjin Dasari, Ramachandra R. So, Peter T. C. Yaqoob, Zahid MIT Laser Biomedical Research Center G.R. Harrison Spectroscopy Laboratory MIT Cambridge MA 02139 United States Mechanical Engineering and Biological Engineering Departments MIT 77 Massachusetts Ave. Cambridge MA 02139 United States

ISBN: (纸本)9780819488701

Self-referenced quantitative phase microscopy (SrQPM) is reported, wherein quantitative phase imaging is achieved through the interference of the sample wave with a reflected version of itself. The off-axis interference between the two beams generates a spatially modulated hologram that is analyzed to quantify the sample's amplitude and phase profile. SrQPM requires approximately one-half of the object field of view to be empty and optically flat, which serves as a reference for the other half of the field of view containing the sample. © 2012 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).

关键词： Holograms

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Multi Angle View of Lung Using Optical Coherence Tomography (OCT)

Multi Angle View of Lung Using Optical Coherence Tomography ...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xix

作者： Golabchi, Ali Gouldstone, Andrew DiMarzio, Charles A. Northeastern Univ Dept Elect & Comp Engn Boston MA 02115 USA Northeastern Univ Dept Engn Mech Boston MA USA Bernard M Gordon Ctr Subsurface Sensing & Imaging Boston MA USA

ISBN: (纸本)9780819488701

Lung imaging, visualization and measurement of alveolar volume has great importance in determining lung health. However, the heterogeneity of lung tissue complicates this task. In this paper multi angle Optical Coherence Tomography (OCT) is used to overcome this problem. One of the limitations of utilizing OCT in lung is the speckle noise and artifacts that originate from the refraction at the tissue-air interface inside the lung. Multi angle view of lung using OCT is incoherent summation of multiple angle-diverse images. Utilizing image registration of multi angle OCT scans of the lung helps reduce the speckle noise and refraction artifacts. This technique helps extract more information from the images which improves visualization and the ability to measure the geometry of alveoli. The other difficulty of utilizing OCT is interpreting the images due to the low numerical aperture (NA) on the OCT. The multi angle view of the lung increases NA, which increase the imaging resolution through synthetic aperture imaging. In this paper inflated excised lung tissue and lung phantom are presented.

关键词： Lung imaging Alveoli Optical Coherence Tomography (OCT) 3D microscopy

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En-face optical coherence imaging for three-dimensional microscopy

En-face optical coherence imaging for three-dimensional micr...

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Conference on 3-dimensional and multidimensional microscopy: image acquisition and processing IX

作者： Akiba, M Chan, KP Tanno, N Japan Sci & Technol Corp Reg Joint Res Project Yamagata 9902473 Japan

ISBN: (纸本)0819443603

En-face optical coherence tomography (OCT) employing parallel heterodyne detection technique is demonstrated for three-dimensional microscopy. To enable the use of commercially available CCD cameras as two-dimensional heterodyne detector arrays in OCT imaging, a frequency synchronous detection method is Employed. Depth-resolved, full-field images are acquired at 30 Hz video-rate without lateral scanning.

关键词： three-dimensional microscopy optical coherence tomography frequency synchronous detection

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Data compression in microscopy: a comparative study

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Proceedings of SPIE - The International Society for Optical Engineering 1999年 3605卷 158-168页

作者： Kriete, Andres Klein, Norbert Berger, Lars C. Inst of Anatomy and Cell Biology Giessen Germany

ISBN: (纸本)0819430757;9780819430755

image compression in microscopy is a valuable technique, in particular if applied to multidimensional data. Information-preserving and competitive information-losing compression is applied to microscopical data and the resulting image quality is evaluated on a quantitative basis both in the spatial and frequency domain. Included are image data featuring different signal-to-noise ratios, but also voxel data for volume representations as well as graphical data for surface representations. The effect of compression on 3-D visualization and image management with data bases is included.

关键词： image compression

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High-resolution three-dimensional imaging using multiple nanometric probes

High-resolution three-dimensional imaging using multiple nan...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Dubois, A Moneron, G Lecaque, R Lequeux, F Boccara, AC Ecole Super Phys & Chim Ind Ville Paris CNRS UPR A0005 Lab Opt Phys F-75231 Paris 5 France

We present a new imaging modality using nanometric beads as multiple local probes. The positions of the beads are determined in three dimensions using white-light interference microscopy, by over-sampling and fitting ... 详细信息

ISBN: (纸本)0819447641

关键词： interference microscopy three-dimensional microscopy scanning probe microscopy

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Real-time quantitative differential interference contrast (DIC) microscopy implemented via novel liquid crystal prisms

Real-time quantitative differential interference contrast (D...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xix

作者： Zahreddine, Ramzi N. Cormack, Robert H. Masterson, Hugh King, Sharon V. Cogswell, Carol J. Univ Colorado Dept Elect Engn 425 UCB Boulder CO 80309 USA Boulder Nonlinear Systems Lafayette CO 80026 USA

ISBN: (纸本)9780819488701

A phase shifting differential interference contrast (DIC) microscope, which provides quantitative phase information and is capable of imaging at video rates, has been constructed. Using a combination of phase shifting and bi-directional shear, the microscope captures a series of eight images which are then integrated in Fourier space. In the resultant image the intensity profile linearly maps to the phase differential across the object. The necessary operations are performed by various liquid crystal devices (LCDs) which can operate at high speeds. A set of four liquid crystal prisms shear the beam in both the x and y directions. A liquid crystal bias cell delays the phase between the e- and o-beams providing phase-shifted images. The liquid crystal devices are then synchronized with a CCD camera in order to provide real-time image acquisition. Previous implementation of this microscope utilized Nomarski prisms, a rotation stage and a manually operated Senarmont compensator to perform the necessary operations and was only capable of fixed sample imaging. In the present work, a series of images were taken using both the new LCD prism based microscope and the previously implemented Senarmont compensator based system. A comparison between these images shows that the new system achieves equal and in some cases superior results to that of the old system with the added benefit of real-time imaging.

关键词： Differential interference contrast (DIC) microscopy quantitative phase imaging liquid crystal devices live cell imaging

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three-dimensional super-resolution imaging of single molecules by spectrally selective confocal microscopy

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Proceedings of SPIE - The International Society for Optical Engineering 1999年 3605卷 82-89页

作者： Brakenhoff, G.J. van Oijen, A.M. Kohler, J. Muller, M. Schmidt, J. Univ of Amsterdam Amsterdam Netherlands

ISBN: (纸本)0819430757;9780819430755

It is shown that based on spectrally selective excitation of individual molecules in the focus of a high NA lens together with position sensitive imaging sub-resolution imaging of three-dimensional structures can be realised. The feasibility of the idea is demonstrated with NA=0.55 optics on a model system of pentacene molecules in p-terphenal host matrix.

关键词： Optical microscopy

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Multi-immersion open-top light-sheet microscopy 27

Multi-immersion open-top light-sheet microscopy

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Glaser, Adam K. Liu, Jonathan T. C. Univ Washington Dept Mech Engn Seattle WA 98195 USA

We present an easy-to-use multi-immersion open-top light-sheet microscope designed specifically for high-throughput imaging of a diverse set of tissues prepared with a variety of clearing protocols.

ISBN: (纸本)9781510632547

We present an easy-to-use multi-immersion open-top light-sheet microscope designed specifically for high-throughput imaging of a diverse set of tissues prepared with a variety of clearing protocols.

关键词： light-sheet microscopy selective-plane illumination microscopy fluorescence microscopy

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