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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xix

作者： Studer, Vincent Bobin, Jerome Chahid, Makhlad Moussavi, Hamed Candes, Emmanuel Dahan, Maxime Univ Bordeaux Interdisciplinary Inst Neurosci UMR 5297 F-33000 Bordeaux France Univ Bordeaux Interdisciplinary Inst Neurosci UMR 5297 F-33000 Bordeaux France CEA Saclay IRFU SEDI Sap Grenoble France Stanford Univ Dept Math Stanford CA 94305 USA Stanford Univ Dept Stat & Elect Engn Stanford CA 94305 USA Univ Paris 06 Ecole Normale Super CNRS Lab Kastler BrosselUMR 8552 Paris France

ISBN: (纸本)9780819488701

In fluorescence microscopy, one can distinguish two kinds of imaging approaches, wide field and raster scan microscopy, differing by their excitation and detection scheme. In both imaging modalities the acquisition is independent of the information content of the image. Rather, the number of acquisitions N, is imposed by the Nyquist-Shannon theorem. However, in practice, many biological images are compressible (or, equivalently here, sparse), meaning that they depend on a number of degrees of freedom K that is smaller that their size N. Recently, the mathematical theory of compressed sensing (CS) has shown how the sensing modality could take advantage of the image sparsity to reconstruct images with no loss of information while largely reducing the number M of acquisition. Here we present a novel fluorescence microscope designed along the principles of CS. It uses a spatial light modulator (DMD) to create structured wide field excitation patterns and a sensitive point detector to measure the emitted fluorescence. On sparse fluorescent samples, we could achieve compression ratio N/M of up to 64, meaning that an image can be reconstructed with a number of measurements of only 1.5 % of its pixel number. Furthemore, we extend our CS acquisition scheme to an hyperspectral imaging system.

关键词： Fluorescence microscopy compressive sensing hyperspectral imaging

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Quantifying fluorescence signals in confocal image stacks deep in turbid media

Quantifying fluorescence signals in confocal image stacks de...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xix

作者： Beer, S. Maeder, U. Bergmann, T. Burg, J. M. Fiebich, M. Runkel, F. Univ Appl Sci Giessen Tech Hsch Mittelhessen Inst Med Phys & Radiat Protect Wiesenstr 14 D-35390 Giessen Germany Univ Appl Sci Giessen Tech Hsch Mittelhessen Inst Biopharmaceut Technol D-35390 Giessen Germany

ISBN: (纸本)9780819488701

When confocal depth stacks are taken, the collected signal (normally the fluorescence signal), decays dependent of the depth of the confocal slice in the turbid medium. This decay is caused by scattering and absorption of the exciting light and of the fluorescence light. As the attenuation parameters, i.e. scattering and absorption coefficients, are normally unknown when observing a new sample, a method is proposed to compensate for the attenuation of the involved light by correcting the fluorescence signal using the attenuation behavior of the sample measured directly on the spot where the fluorescence stack is taken. The method works without any a priori knowledge about the optical properties of the sample. Using this self-reference technique, a confocal fluorescence depth stack can be created where the signal intensity is not dependent on the scattering and absorption caused intensity decay. The proposed method is tested on fluorescent beads embedded in scattering and absorbing hydrogel phantoms.

关键词： confocal microscopy image processing signal correction

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Multi Angle View of Lung Using Optical Coherence Tomography (OCT)

Multi Angle View of Lung Using Optical Coherence Tomography ...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xix

作者： Golabchi, Ali Gouldstone, Andrew DiMarzio, Charles A. Northeastern Univ Dept Elect & Comp Engn Boston MA 02115 USA Northeastern Univ Dept Engn Mech Boston MA USA Bernard M Gordon Ctr Subsurface Sensing & Imaging Boston MA USA

ISBN: (纸本)9780819488701

Lung imaging, visualization and measurement of alveolar volume has great importance in determining lung health. However, the heterogeneity of lung tissue complicates this task. In this paper multi angle Optical Coherence Tomography (OCT) is used to overcome this problem. One of the limitations of utilizing OCT in lung is the speckle noise and artifacts that originate from the refraction at the tissue-air interface inside the lung. Multi angle view of lung using OCT is incoherent summation of multiple angle-diverse images. Utilizing image registration of multi angle OCT scans of the lung helps reduce the speckle noise and refraction artifacts. This technique helps extract more information from the images which improves visualization and the ability to measure the geometry of alveoli. The other difficulty of utilizing OCT is interpreting the images due to the low numerical aperture (NA) on the OCT. The multi angle view of the lung increases NA, which increase the imaging resolution through synthetic aperture imaging. In this paper inflated excised lung tissue and lung phantom are presented.

关键词： Lung imaging Alveoli Optical Coherence Tomography (OCT) 3D microscopy

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Self-referenced quantitative phase microscopy

Self-referenced quantitative phase microscopy

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three-dimensional and multidimensional microscopy: image acquisition and processing xix

作者： Hillman, Timothy R. Lue, Niyom Sung, Yongjin Dasari, Ramachandra R. So, Peter T. C. Yaqoob, Zahid MIT Laser Biomedical Research Center G.R. Harrison Spectroscopy Laboratory MIT Cambridge MA 02139 United States Mechanical Engineering and Biological Engineering Departments MIT 77 Massachusetts Ave. Cambridge MA 02139 United States

ISBN: (纸本)9780819488701

Self-referenced quantitative phase microscopy (SrQPM) is reported, wherein quantitative phase imaging is achieved through the interference of the sample wave with a reflected version of itself. The off-axis interference between the two beams generates a spatially modulated hologram that is analyzed to quantify the sample's amplitude and phase profile. SrQPM requires approximately one-half of the object field of view to be empty and optically flat, which serves as a reference for the other half of the field of view containing the sample. © 2012 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).

关键词： Holograms

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En-face optical coherence imaging for three-dimensional microscopy

En-face optical coherence imaging for three-dimensional micr...

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Conference on 3-dimensional and multidimensional microscopy: image acquisition and processing IX

作者： Akiba, M Chan, KP Tanno, N Japan Sci & Technol Corp Reg Joint Res Project Yamagata 9902473 Japan

ISBN: (纸本)0819443603

En-face optical coherence tomography (OCT) employing parallel heterodyne detection technique is demonstrated for three-dimensional microscopy. To enable the use of commercially available CCD cameras as two-dimensional heterodyne detector arrays in OCT imaging, a frequency synchronous detection method is Employed. Depth-resolved, full-field images are acquired at 30 Hz video-rate without lateral scanning.

关键词： three-dimensional microscopy optical coherence tomography frequency synchronous detection

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High-resolution three-dimensional imaging using multiple nanometric probes

High-resolution three-dimensional imaging using multiple nan...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Dubois, A Moneron, G Lecaque, R Lequeux, F Boccara, AC Ecole Super Phys & Chim Ind Ville Paris CNRS UPR A0005 Lab Opt Phys F-75231 Paris 5 France

We present a new imaging modality using nanometric beads as multiple local probes. The positions of the beads are determined in three dimensions using white-light interference microscopy, by over-sampling and fitting ... 详细信息

ISBN: (纸本)0819447641

关键词： interference microscopy three-dimensional microscopy scanning probe microscopy

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Data compression in microscopy: a comparative study

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Proceedings of SPIE - The International Society for Optical Engineering 1999年 3605卷 158-168页

作者： Kriete, Andres Klein, Norbert Berger, Lars C. Inst of Anatomy and Cell Biology Giessen Germany

ISBN: (纸本)0819430757;9780819430755

image compression in microscopy is a valuable technique, in particular if applied to multidimensional data. Information-preserving and competitive information-losing compression is applied to microscopical data and the resulting image quality is evaluated on a quantitative basis both in the spatial and frequency domain. Included are image data featuring different signal-to-noise ratios, but also voxel data for volume representations as well as graphical data for surface representations. The effect of compression on 3-D visualization and image management with data bases is included.

关键词： image compression

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three-dimensional multiple nanometric local probes microscopy

Three-dimensional multiple nanometric local probes microscop...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Moneron, G Dubois, A Boccara, AC CNRS UPA A0005 Ecole Super Phys Chim Ind Lab Opt Phys F-75231 Paris France

ISBN: (纸本)0819456756

We present a microscope set-up designed to produce three-dimensional images of the internal structures of various samples with high spatial resolution (a few tens of nanometers in axial and transverse directions). This level of resolution is reached by the use of nanometric sub-wavelength spherical gold beads as multiple local probes, dispersed in the hollow structures. The exploration, by Brownian motion, of the internal structures allows their three-dimensional reconstruction.

关键词： interference microscopy three-dimensional microscopy optical coherence tomography white-light interferometry scanning probe microscopy

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Real-time quantitative differential interference contrast (DIC) microscopy implemented via novel liquid crystal prisms

Real-time quantitative differential interference contrast (D...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xix

作者： Zahreddine, Ramzi N. Cormack, Robert H. Masterson, Hugh King, Sharon V. Cogswell, Carol J. Univ Colorado Dept Elect Engn 425 UCB Boulder CO 80309 USA Boulder Nonlinear Systems Lafayette CO 80026 USA

ISBN: (纸本)9780819488701

A phase shifting differential interference contrast (DIC) microscope, which provides quantitative phase information and is capable of imaging at video rates, has been constructed. Using a combination of phase shifting and bi-directional shear, the microscope captures a series of eight images which are then integrated in Fourier space. In the resultant image the intensity profile linearly maps to the phase differential across the object. The necessary operations are performed by various liquid crystal devices (LCDs) which can operate at high speeds. A set of four liquid crystal prisms shear the beam in both the x and y directions. A liquid crystal bias cell delays the phase between the e- and o-beams providing phase-shifted images. The liquid crystal devices are then synchronized with a CCD camera in order to provide real-time image acquisition. Previous implementation of this microscope utilized Nomarski prisms, a rotation stage and a manually operated Senarmont compensator to perform the necessary operations and was only capable of fixed sample imaging. In the present work, a series of images were taken using both the new LCD prism based microscope and the previously implemented Senarmont compensator based system. A comparison between these images shows that the new system achieves equal and in some cases superior results to that of the old system with the added benefit of real-time imaging.

关键词： Differential interference contrast (DIC) microscopy quantitative phase imaging liquid crystal devices live cell imaging

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three-dimensional imagery using multiple nanometric probes

Three-dimensional imagery using multiple nanometric probes

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Moneron, G Dubois, A Boccara, AC Ecole Super Phys & Chim Ind Ville Paris Lab Opt Phys CNRS UPR A0005 F-75231 Paris 5 France

ISBN: (纸本)0819452327

We present a high-resolution imaging technique using nanometric beads as multiple scattering local probes. The positions of the beads are determined in three dimensions by white-light interference microscopy. The technique has been applied to study the deformation of gels under mechanical constraint. The location of Brownian moving beads has also been demonstrated with nanometer spatial precision and 10 mus acquisition time. High-resolution 3-D imaging of hollow structures explored by the beads in relatively transparent materials should be possible.

关键词： interference microscopy three-dimensional microscopy scanning probe microscopy

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