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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX"

共 592 条记录，以下是41-50 订阅

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Evaluation of different illumination designs for tunable 3D structured illumination microscopy through model-based restoration 28

Evaluation of different illumination designs for tunable 3D ...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXVIII 2021

作者： Mohammed, Jules Van, Cong T. S. Preza, Chrysanthe Computational Imaging Research Laboratory Department of Electrical and Computer Engineering University of Memphis MemphisTN38152 United States

ISBN: (数字)9781510641341

ISBN: (纸本)9781510641334

In a tunable 3D structured illumination microscopy (3D-SIM) based on an illumination system comprised by a multi-slit array and a Fresnel biprism, the 3D structured illumination (SI) pattern depends on the design of the slit array. Previous work studied the impact of the illumination pattern design on the achieved extension of the compact support of the tunable 3D-SIM optical transfer function. In this contribution, we use simulations with different illumination designs that utilize a different number of slits, to evaluate system performance by investigating the lateral and axial resolution achieved in the restored images computed with a regularized iterative 3D model-based algorithm, which minimizes the mean square error between the model and data. three illumination designs are investigated in which the number of slits is N = 3, 9 and 11 while the corresponding distance between the slits is x0 = 488 μm, 122 μm and 100 μm, respectively. A 3D star-like object, commonly used in resolution analysis of imaging systems, is used in the simulations. Results are quantified using the mean square error and the structured similarity index as well as intensity profiles that show the achieved resolution and robustness to noise in each case. The lateral and axial 3D-SIM theoretical resolution limits are achieved in the presence of 20-dB Poisson noise while small differences are evident in the results from 15-dB simulated data obtained using the 3 investigated designs. © 2021 Copyright SPIE.

关键词： Fluorescence microscopy

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Deconvolution optical-resolution photoacoustic microscope for high-resolution imaging of cerebral vasculature 28

Deconvolution optical-resolution photoacoustic microscope fo...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXVIII 2021

作者： Song, Xianlin Song, Lingfang Chen, Anqing Wei, Jianshuang Zhao, Aojie Teng, Ao School of Information Engineering Nanchang University Nanchang330031 China Nanchang Normal University Nanchang330031 China Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan430074 China Moe Key Laboratory of Biomedical Photonics of Ministry of Education Department of Biomedical Engineering Huazhong University of Science and Technology Wuhan430074 China

ISBN: (数字)9781510641341

ISBN: (纸本)9781510641334

Photoacoustic imaging is becoming a very promising tool for the research of living organisms. It combines the high contrast of optical imaging and the high resolution of acoustic imaging to realize the imaging of absorption clusters in biological tissues. Since the scattering of ultrasound signals in biological tissues is 2-3 orders of magnitude weaker than the scattering of light in biological tissues, the endogenous absorption difference of tissues is directly used in the imaging process, so photoacoustic imaging has the advantages of deep imaging depth and non-destructive. As an important branch of photoacoustic imaging, photoacoustic microscopy can provide micron-level or even sub-micron-level imaging resolution, which is of great significance for biological research such as blood vessel detection. Since the lateral resolution of the photoacoustic microscopy imaging system depends on the focus of the laser, a higher resolution can be obtained by increasing the numerical aperture of the condenser objective. However, a large numerical aperture usually means a shorter working distance and makes the entire imaging system very sensitive to small optical defects. Therefore, the improvement of resolution through this method will be limited in practical applications. This paper implements a method of using iterative deconvolution to obtain a high-resolution photoacoustic image of the brain. The focal spot of the photoacoustic microscopy is measured to obtain the lateral PSF (point spread function) of the system. Making the measured PSF as the initial system PSF to perform Lucy-Richardson (LR) deconvolution. The image resolution of cerebral vasculature obtained by this method is higher. The full width at half maximum (FWHM) of width of the same cerebral capillaries before and after deconvolution are 7 μm and 3.6 μm, respectively, and the image definition is increased by about 1.9 times. Experiments show that this method can further improve the clarity of photoacoustic im

关键词： Photoacoustic microscopy

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Multi-immersion open-top light-sheet microscopy 27

Multi-immersion open-top light-sheet microscopy

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Glaser, Adam K. Liu, Jonathan T. C. Univ Washington Dept Mech Engn Seattle WA 98195 USA

We present an easy-to-use multi-immersion open-top light-sheet microscope designed specifically for high-throughput imaging of a diverse set of tissues prepared with a variety of clearing protocols.

ISBN: (纸本)9781510632547

We present an easy-to-use multi-immersion open-top light-sheet microscope designed specifically for high-throughput imaging of a diverse set of tissues prepared with a variety of clearing protocols.

关键词： light-sheet microscopy selective-plane illumination microscopy fluorescence microscopy

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Auto-correlation for multi-view deconvolved reconstruction in light sheet microscopy 28

Auto-correlation for multi-view deconvolved reconstruction i...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXVIII 2021

作者： Ancora, Daniele Valentini, Gianluca Pifferi, Antonio Giovanni Bassi, Andrea Dipartimento di Fisica Politecnico di Milano Piazza Leonardo da Vinci 32 Milano20133 Italy Istituto di Fotonica e Nanotecnologie Consiglio Nazionale delle ricerche Piazza Leonardo da Vinci 32 Milano20133 Italy

ISBN: (数字)9781510641341

ISBN: (纸本)9781510641334

Tomographic inspection of fluorescent labels distributed within a specimen is an important aspect in biology. Light sheet fluorescent microscopy (LSFM) offers a powerful and simple tool to selectively slice the sample and let us directly obtain a tomographic view of the specimen. However, due to non-isotropic resolution of the technique along the axial scanning, one may want to combine different views of the object and add deconvolution to the process in order to achieve higher resolution. Typically, multi-view Bayesian methods based on Richardson-Lucy deconvolution are used for this task once the datasets are exactly registered against each other. In this work, instead, we begin to investigate how to avoid the alignment procedure and use a direct algorithm to form a multi-view tomographic reconstruction. To do this, we developed a new framework based on auto-correlation analysis that let us achieve deconvolved reconstructions starting from blurred auto-correlations. Since the latter are insensitive to shifts, we can combine the auto-correlations coming from multi-view acquisitions without taking care of the registration procedure. © 2021 Copyright SPIE.

关键词： Inverse problems

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Computational super-resolution microscopy: leveraging noise model, regularization and sparsity to achieve highest resolution 27

Computational super-resolution microscopy: leveraging noise ...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Xing, Jian Chen, Simeng Becker, Stephen Yu, Jiun-Yann Cogswell, Carol Univ Colorado Dept Elect Comp & Energy Engn Boulder CO 80309 USA Univ Colorado Dept Appl Math Boulder CO 80309 USA

ISBN: (纸本)9781510632547

We report progress in algorithm development for a computation-based super-resolution microscopy technique. Building upon previous results, we examine our recently implemented microscope system and construct alternative processing algorithms. Based on numerical simulations results, we evaluate the performance of each algorithm and determine the one most suitable for our super-resolution microscope.

关键词： Super-resolution microscopy regularization computational microscopy

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Method for measurement of collagen monomer orientation in fluorescence microscopy 28

Method for measurement of collagen monomer orientation in fl...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXVIII 2021

作者： Alzola, Rodrigo P. Delpiano, Jose Gajjar, Anuj Stureborg, Rickard Siadat, Seyed Mohammad Ruberti, Jeffrey W. Dimarzio, Charles A. Department of Electrical and Computer Engineering Northeastern University BostonMA United States Optical Communications Lab School of Engineering and Applied Sciences Universidad de los Andes Santiago Chile Department of Bioengineering Northeastern University BostonMA United States Department of Mechanical and Industrial Engineering Northeastern University BostonMA United States

ISBN: (数字)9781510641341

ISBN: (纸本)9781510641334

Collagen is one of the most important proteins in mammals, conforming most animal tissues. This work explores how a basic collagen monomer unit is visualized using fluorescence microscopy and how its spatial orientation is determined. Defining the orientation of collagen monomers is not a trivial problem, as the particle has a weak contrast and is relatively small. Possible attach fluorescence tags for contrast, but the size is still a problem for detecting orientation using fluorescence microscopy. This document presents a simulation of the visualization of collagen monomers and two methods for detecting monomer and classifying its orientation. A modify Gabor filter set, and an automatic classifier, trained by convolutional neuronal network (CNN), were used. By evaluating the performance of these two approaches compare to human observation, our results show that it is possible to determine the location and orientation of a single monomer with fluorescence microscopy. These findings can contribute to understanding collagen elements as collagen fibril. © 2021 Copyright SPIE.

关键词： Collagen

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A study for accelerating the speed of all-in-focus image processing 27

A study for accelerating the speed of all-in-focus image pro...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Wang, Lihui Tabata, Satoshi Takeuchi, Hirotoshi Ishikawa, Masatoshi Guangdong Acad Sci Guangdong Inst Semicond Ind Technol 363 Changxing Rd Guangzhou 510650 Peoples R China Univ Tokyo Grad Sch Informat Sci & Technol Bunkyo Ku 7-3-1 Hongo Tokyo 1138656 Japan

ISBN: (纸本)9781510632547

To capture an all-in-focus and 3D depth image, shape from focus method is widely used. The phase accuracy of the image candidates should be processed and adjusted beforehand Phase only correction method was employed in this work and to accelerate the processing speed, the image processing by Fast Fourier Transform (FFT) was optimized Meanwhile, the processing task was assigned in several parallel threads so that the performance would be improved. The method was used on a variable focus imaging system, and, as a result, the processing speed was improved to around 2.5-fps.

关键词： all-in-focus shape from focus phase-only correction method 3D imaging variable focus system

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3,3′-thiodipropanol as a refractive index matching mounting medium for fluorescence microscopy 27

3,3′-thiodipropanol as a refractive index matching mounting...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Alata Tejedo, Milvia Iris Martinez Cervantes, Juan Carlos Piazza, Valeria Ctr Invest Opt AC Loma Bosque 115 Guanajuato Mexico

ISBN: (纸本)9781510632547

In this work, several tests of the performance of 3,3'-thiodipropanol (TDP) as a mounting medium for fluorescence microscopy of biological samples were performed. Besides optical properties like the dispersion curve of TDP and the effect of the embedding medium on the fluorescence of commonly used dyes, the interaction with biological specimens, including the labeling of filamentous actin with fluorescent phalloidins, was tested: TDP showed to represent an interesting alternative to commercial mounting media. By mixing TDP with 2,2'-thiodiethanol (TDE), it was possible not only to fine tune the refractive index of the resulting solution, but to preserve the compatibility with fluorescent phalloidins.

关键词： Mounting media fluorescence microscopy filamentous actin

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Multifocus imaging via polarization wavefront shaping 27

Multifocus imaging via polarization wavefront shaping

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Li, Chen Ding, Changqin Li, Minghe Rong, Jiayue Ulcickas, James Simpson, Garth J. Purdue Univ Dept Chem W Lafayette IN 47907 USA

ISBN: (纸本)9781510632547

Conventional three-dimensional (3D) images of biological samples are typically assembled from a stack of two-dimensional images acquired sequentially at different focal planes. This time-consuming manner hinders the application of 3D imaging techniques to the investigation of fast biochemical dynamics and light-sensitive biological events. The concept of multifocus imaging, which enables simultaneous acquisition of images from multiple focal planes, was introduced to achieve rapid 3D imaging. In the present study, we achieved multifocus imaging through polarization wavefront shaping via a micro-retarder array which splits the incident linearly polarized light into three beamlets that are focused to three axially-offset focal planes with 100 itm separation. Append to an existing beam-scanning microscope, this multifocus system enables rapid 3D imaging compatible with a variety of optical microscopic approaches including laser transmittance, two-photon excited fluorescence, and second harmonic generation microscopy.

关键词： Multifocus imaging beam scanning microscopy polarization wavefront shaping Jones calculus

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FPGA Implementation of Spectral Fusing Gabor Domain Optical Coherence microscopy 27

FPGA Implementation of Spectral Fusing Gabor Domain Optical ...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXVII

作者： Meemon, Panomsak Lenaphet, Yutana Saetiew, Jadsada Suranaree Univ Technol Sch Phys Inst Sci Nakhon Ratchasima 30000 Thailand Suranaree Univ Technol Sch Elect Engn Inst Engn Nakhon Ratchasima 30000 Thailand

ISBN: (纸本)9781510632547

Gabor domain optical coherence microscopy (GD-OCM) is an emerging tomographic imaging technique that overcome the depth of focus (DOF) limit of high numerical objective lens by acquiring multiple cross-section images corresponded with different focal planes of the objective along an axial direction and combining them to form an invariant high resolution cross-section image. We previously reported an alternative processing method of GD-OCM called spectral fusing GD-OCM (SF-GD-OCM). SF-GD-OCM extracts in-focus information directly from acquired spectral interference signals. The advantage of SF-GD-OCM over the original GD-OCM is that the Gabor filtering and fusing can be implemented in an acquisition hardware instead of a host computer. Here, we have investigated the implementation of SF-GD-OCM by using FPGA frame grabber. The experimental setup of the acquisition system as well as the implementation of the spectral Gabor fusion in FPGA device have been developed and verified. Results show that the data processing of SF-GD-OCM in FPGA enabled acquisition device is possible and could lead to improvement in overall imaging speed of GD-OCM.

关键词： Gabor domain optical coherence microscopy Gabor fusion technique FPGA Extended depth of field

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