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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX"

共 592 条记录，以下是551-560 订阅

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Data compression in microscopy: a comparative study

Data compression in microscopy: a comparative study

引用

Proceedings of the 1999 three-dimensional and multidimensional microscopy: image acquisition and processing VI

作者： Kriete, Andres Klein, Norbert Berger, Lars C. Inst of Anatomy and Cell Biology Giessen Germany

ISBN: (纸本)0819430757

image compression in microscopy is a valuable technique, in particular if applied to multidimensional data. Information-preserving and competitive information-losing compression is applied to microscopical data and the resulting image quality is evaluated on a quantitative basis both in the spatial and frequency domain. Included are image data featuring different signal-to-noise ratios, but also voxel data for volume representations as well as graphical data for surface representations. The effect of compression on 3-D visualization and image management with data bases is included.

关键词： image compression

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Confocal laser microscopy of the in vivo human skin revisited.

Confocal laser microscopy of the <i>in vivo</i> human skin r...

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VI

作者： Corcuff, P Hadjur, C Chaussepied, C Toledo-Crow, R LOreal Labs Rech Avancee F-93601 Aulnay Sous Bois France

ISBN: (纸本)0819430757

A new generation of confocal laser microscope, designed to image the human skin in vivo, improves the resolution, contrast and spectroscopic facilities as compared to the previous Tandem Scanning Microscope (TSM) prototype. The new device has been built with a Oz module (Noran) equipped with the skin contact device, assuming a perfect stability of skin images in the horizontal plane. The Z displacement of the objective lens, mounted directly on the Ch module, is assumed by a piezo motor with a course of 350 mu m. Moreover, the Ch module has been suspended on articulated arms to reach any part of the human body. The power of the Argon/Krypton laser source has been limited to 2 mW to secure safety and provides three visible wavelength : 488, 568 and 647 nm. The facility of instantly checking wavelength during in depth exploration of the skin optimizes the resolution and contrast of images as compared with the white light used in the TSM. Consequently, better image quality of the epidermis is obtained in the blue region with unexpected details of corneocytes and keratinocytes. The papillary dermis comprising the vascular network is advantageously observed with the red light. The fluorescence channel detector gives additional information on the penetration of fluorescent probes through the skin barrier. Optical sections are digitized (512 x 480 x 8 bit) at video rate, providing easy and fast measurements of the thickness of epidermal layers. The Silicon Graphics workstation generates a transparent volume of living human skin in less than 5 minutes. This powerful and convivial new design for imaging the in vivo human skin opens up new promises in skin research and in vivo skin pharmacology.

关键词： confocal microscopy human skin in vivo imaging fluorescence volume rendering

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Confocal photoluminescence microscopy in II-VI materials: annealing and degradation dynamics

Confocal photoluminescence microscopy in II-VI materials: an...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VI

作者： McCabe, EM Jordan, C Fewer, DT Donegan, JF Taniguchi, S Hino, T Nakano, K Ishibashi, A Uusimaa, P Pessa, M Univ Dublin Trinity Coll Dublin 2 Ireland

ISBN: (纸本)0819430757

Confocal photoluminescence imaging is an important tool in the investigation of recombination in semiconductors and in the characterisation of material growth. This characterisation is particularly important for II-VI wide band-gap semiconductors where the potential for blue-green lasers is being explored currently. To achieve room-temperature cw operation of these lasers over the multi-thousand hours necessary for commercialisation, extremely low defect densities are required. The confocal microscope is used in this work to image photoluminescence from II-VI materials to characterise the defect formation and propagation within the quantum well region of the material. This imaging approach permits the degradation to be monitored in real time and over a large area in samples with low defect densities. The additional advantages of this set-up over a conventional microscope are, of course, the higher lateral resolution and narrow depth of field associated with a confocal microscope. While considerable effort has been focused on the degradation in these II-VI semiconductors, we have recently observed that annealing can occur simultaneously in the same sample when the material is exposed to intense optical excitation. images of annealing and degradation of a range of II-VI samples will be presented to highlight these observations.

关键词： confocal microscopy photoluminescence imaging semiconductors II-VI materials blue green lasers

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Imaging modalities in confocal correlation microscopy

Imaging modalities in confocal correlation microscopy

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Proceedings of the 1999 three-dimensional and multidimensional microscopy: image acquisition and processing VI

作者： Juskaitis, Rimas Neil, Mark A.A. Wilson, Tony Oxford Univ Oxford United Kingdom

ISBN: (纸本)0819430757

We present a novel light efficient technique for obtaining optically sectioned images in wide-field microscopy. The technique is a further development of correlation microscopy and is based on using complementary structured light patterns to illuminate the specimen together with uncomplicated post-processing of the captured images.

关键词： Optical microscopy

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Parallel, confocal, and complete spectrum imager for fluorescent detection of high-density microarray

Parallel, confocal, and complete spectrum imager for fluores...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VI

作者： Bogdanov, V Boyce-Jacino, M Orchid Biocomp Inc Baltimore MD 21224 USA

ISBN: (纸本)0819430757

Confined arrays of biochemical probes deposited on a solid support surface (analytical microarray or "chip") provide an opportunity to analysis multiple reactions simultaneously. Microarrays are increasingly used in genetics, medicine and environment scanning as research and analytical instruments. A power of microarray technology comes from its parallelism which grows with array miniaturization, minimization of reagent volume per reaction site and reaction multiplexing. An optical detector of microarray signals should combine high sensitivity, spatial and spectral resolution. Additionally, low-cost and a high processing rate are needed to transfer microarray technology into biomedical practice. We designed an imager that provides confocal and complete spectrum detection of entire fluorescently-labeled microarray in parallel. imager uses microlens array, non-slit spectral decomposer, and high-sensitive detector (cooled CCD). Two imaging channels provide a simultaneous detection of localization, integrated and spectral intensities for each reaction site in microarray. A dimensional matching between microarray and imager's optics eliminates all in moving parts in instrumentation, enabling highly informative, fast and low-cost microarray detection. We report theory of confocal hyperspectral imaging with microlenses array and experimental data for implementation of developed imager to detect fluorescently labeled microarray with a density similar to 10(3) sites per cm(2).

关键词： microarray technology fluorescence confocal scanning two-dimensional spectroscopy

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Feature extraction and 3D reconstruction of Nomarski DIC microscope images using local energy and neural net techniques

Feature extraction and 3D reconstruction of Nomarski DIC mic...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VI

作者： Kyan, M Guan, L Arison, M Cogswell, C Univ Sydney Dept Elect Engn Sydney NSW 2006 Australia

ISBN: (纸本)0819430757

The Nomarski differential interference contrast (DIC) mode is commonly used for imaging translucent biological specimens and it exhibits several major advantages over other phase contrast techniques, including a boost in high spatial frequencies in the region of focus. However, DIC images (unlike confocal) are limited by the presence of low spatial frequency blur and also by a differential shading gradient at feature boundaries, which make normal confocal visualisation techniques unsuitable for feature extraction or for 3D volume rendering of focus-series datasets. To remedy this problem, we employ a neural network technique based on competitive learning, known as Kohonen's self-organising feature map (SOFM), to perform segmentation, using a collection of statistics (know as features) defining the image. Our past investigation showed that standard features such as the localised mean and variance of pixel intensities provided reasonable extraction of objects such asmitotic chromosomes, but surface detail was only moderately resolved. In this current work, local energy is investigated as an alternative image statistic based on phase congruency in the image. This, along with different combinations of other image statistics, is applied in a SOFM, producing 3D images exhibiting vast improvement in the level of detail and clearly isolating the chromosomes from the background.

关键词： differential interference contrast DIC feature extraction feature space image segmentation self organising feature map SOFM phase congruency local energy Morlet wavelet

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System for fluorescence lifetime imaging microscopy

System for fluorescence lifetime imaging microscopy

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Proceedings of the 1999 three-dimensional and multidimensional microscopy: image acquisition and processing VI

作者： van Geest, Lambertus K. Boddeke, Frank R. van Dijk, Pieter W. Kamp, Arjen F. van der Oord, Cornelis J.R. Stoop, Karel W.J. Lambert Instruments Netherlands

ISBN: (纸本)0819430757

An affordable, robust and easy-to-use fluorescence lifetime imaging microscopy (FLIM) workstation that is completely automated and does not need any difficult calibration procedure is introduced. The workstation consists of a standard fluorescence microscope, a modulated excitation light source, a camera, a modulation signal generator and acquisition/processing software. An evaluation of the system and its critical components is presented.

关键词： Optical microscopy

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Developments and applications of confocal theta microscopy

Developments and applications of confocal theta microscopy

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Proceedings of the 1999 three-dimensional and multidimensional microscopy: image acquisition and processing VI

作者： Haar, Frank-Martin Swoger, Jim Stelzer, Ernst H.K. Heidelberg Germany

ISBN: (纸本)0819430757

Confocal theta microscopy improves the resolution of confocal laser scanning microscopes by instrumentally solving the problem of the inferior axial resolution. A technical variation of this microscopy technique, the Single-Lens Theta microscopy (SLTM), is designed to be easily adapted to any common confocal laser scanning microscope. With SLTM, different kinds of other microscopical techniques are possible.

关键词： Optical microscopy

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three-dimensional reconstruction and analysis of gastric malignancies by electronic slides of consecutive sections and virtual microscopy

Three-dimensional reconstruction and analysis of gastric mal...

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Proceedings of the 1999 three-dimensional and multidimensional microscopy: image acquisition and processing VI

作者： Molnar, Bela Papik, Kornel Tagscherer, Attila Csendes, Gabor Varga, Sebestyen V. Tulassay, Zsolt Schaefer, Rainer Mahoney, Walt Semmelweis Univ Budapest Hungary

ISBN: (纸本)0819430757

A study was carried out focusing on the application of an electronic slide and a software simulated virtual microscope to histological analysis. The electronic slide was developed using a fully computer controlled microscope, video camera and an Intel Pentium II based PC. A program called Pyramid was developed for the scanning of an entire slide. Autofocusing, image reduction and frame joining algorithms were implemented in the virtual microscope application. Results confirm that the electronic slide and the software contribute to a more efficient, convenient histological analysis.

关键词： Optical microscopy

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Fast IR imaging with sub-wavelength resolution using a transient near-field probe (tipless near-field microscopy)

Fast IR imaging with sub-wavelength resolution using a trans...

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Proceedings of the 1999 three-dimensional and multidimensional microscopy: image acquisition and processing VI

作者： Palanker, Daniel V. Smith, Todd I. Schwettman, H.Alan Stanford Univ Stanford CA United States

ISBN: (纸本)0819430757

A technique that enables infrared (IR) imaging with the resolution of a visible microscope is introduced. The technique uses conventional IR and no near-field tips. The resolution of the IR microscope is determined by the dimensions of the transient mirror. To prevent resolution degradation due to diffusion of the photo-excited carriers in the substrate, the probe (IR) pulse duration should not exceed a few tens of picoseconds.

关键词： Optical microscopy

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