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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX"
592 条 记 录,以下是571-580 订阅
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Optically sectioned images in widefield fluorescence microscopy
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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V
作者: Wilson, T Neil, MAA Juskaitis, R Univ Oxford Dept Engn Sci Oxford OX1 3PJ England
An analysis is presented of the image formation in widefield fluorescence microscopy using standard light. The region of support of the resulting optical transfer function is discussed.
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image enhancement for a low cost TEM acquisition system.
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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V
作者: Randall, G Fernandez, A Trujillo-Cenoz, O Apelbaum, G Bertalmio, M Vazquez, L Malmierca, F Morelli, P Univ Republ Fac Ingn Inst Ingn Elect Montevideo Uruguay
This paper describes a method for the improvement of biological images acquired using a Transmission Electronic Microscope (TEM). Several techniques are presented that deal with noise reduction, artifact removal and n... 详细信息
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three-dimensional reconstruction by focus sensing from conventional 2D image slices
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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V
作者: Nahm, KB Shin, ES Ryoo, SM Hallym Univ Dept Phys Chunchon 200702 Kangwon South Korea
We introduce an optical means of reconstructing the topology of rough objects observed under the microscope or at a relatively high magnification. First, 2D images were obtained under the conventional optical microsco... 详细信息
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microscopy using an interference pattern as illumination source
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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V
作者: Juskaitis, R Neil, MAA Wilson, T Univ Oxford Dept Engn Sci Oxford OX1 3PJ England
We describe a method of obtaining optically sectioned fluorescence images in a widefield conventional microscope by interfering two beams on an object so as to illuminate it with a single spatial frequency fringe patt... 详细信息
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Polarised light microscopy
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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V
作者: Torok, P Higdon, PD Wilson, T Univ Oxford Dept Engn Sci Oxford OX1 3PJ England
In this work we discuss the role of polarisation in confocal microscopy. The effect of the optical system on light polarisation is analysed in the absence of a specimen. We also present numerical results on confocal m... 详细信息
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Comparing methods for 3D microscopy
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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V
作者: McNally, JG Markham, J Conchello, JA Washington Univ Inst Biomed Comp St Louis MO 63110 USA
In previous studies, we examined three-dimensional images of a latex bead containing a surface layer of fluorescence whose thickness was determined by physical sectioning. Confocal images of the bead from six differen... 详细信息
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Transmission confocal microscopy: making it a reality
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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V
作者: Arnison, MR Fekete, PW Serrano, M Nguyen, PTA Romagnoli, R Guan, L O'Byrne, JW Cogswell, CJ Univ Sydney Sch Phys Dept Phys Opt Sydney NSW 2006 Australia
Imaging thick specimens in 3D transmission confocal modes presents two key problems. The first problem is variable aberrations introduced by changes in refractive index. The second problem is revealed when visualising... 详细信息
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three-dimensional limited-angle microtomography of blood cells: experimental results
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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V
作者: Levin, GG Vishnyakov, GN Zakarian, CS Likhachov, AV Pickalov, VV Kozinets, GI Novoderzhkina, JK Streletskaya, EA Russian Res Inst Optophys Measurements Moscow 119361 Russia
Tomographic measurements of the 3D refractive index spatial distribution within optically transparent phase samples with computerized interferometric microscopes are proposed. Phase shifting interferometric microtomog... 详细信息
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Improved optical method for measuring of AFM cantilever deflection
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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V
作者: Baibyrin, VB Konnov, NP Volkov, UP Saratov Tech Univ Saratov 410016 Russia
In our lab have been developed an atomic force microscope (AFIM) for biological and technical applications with improved optical method for measuring of cantilever deflection. We use conventional technique of measurin... 详细信息
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acquisition of multiple image stacks with a confocal laser scanning microscope
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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing V
作者: Zuschratter, W Steffen, T Braun, K Herzog, A Michaelis, B Scheich, H Fed Inst Neurobiol D-39118 Magdeburg Germany
image acquisition at high magnification is inevitably correlated with a limited view over the entire tissue section. To overcome this limitation we designed software for multiple image-stack acquisition (3D-MISA) in c... 详细信息
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