An analysis is presented of the image formation in widefield fluorescence microscopy using standard light. The region of support of the resulting optical transfer function is discussed.
ISBN:
(纸本)0819427004
An analysis is presented of the image formation in widefield fluorescence microscopy using standard light. The region of support of the resulting optical transfer function is discussed.
A hyperspectral imager provides a 3-D data cube in which the spatial information (2-D) of the image is complemented by spectral information (1-D) about each spatial location. A static, high-throughput spectrometer des...
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ISBN:
(纸本)0819461326
A hyperspectral imager provides a 3-D data cube in which the spatial information (2-D) of the image is complemented by spectral information (1-D) about each spatial location. A static, high-throughput spectrometer design previously developed by our group can be used as the spectral engine in a high-throughput hyperspectral imager that avoids the Fourier undersampling issues present in previous dispersive designs. We present the theory for both pushbroom and tomographic operation and describe experimental results from our proof-of-concept implementation of a hyperspectral microscope.
We introduce an optical means of reconstructing the topology of rough objects observed under the microscope or at a relatively high magnification. First, 2D images were obtained under the conventional optical microsco...
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ISBN:
(纸本)0819427004
We introduce an optical means of reconstructing the topology of rough objects observed under the microscope or at a relatively high magnification. First, 2D images were obtained under the conventional optical microscope or under the macroscopic imaging system, focused on differing height of the sample. The depth information was extracted by sensing the focus of each pixel of the conventional image slice. Each sample height associated with the best "focus figure" for each pixel was marked and were later utilized to generate the threedimensional coordinate map.
We present a novel light efficient technique for obtaining optically sectioned images in wide-field microscopy. The technique is a further development of correlation microscopy and is based on using complementary stru...
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ISBN:
(纸本)0819430757;9780819430755
We present a novel light efficient technique for obtaining optically sectioned images in wide-field microscopy. The technique is a further development of correlation microscopy and is based on using complementary structured light patterns to illuminate the specimen together with uncomplicated post-processing of the captured images.
An approximate model for optical-sectioning microscopy describing depth-varying imaging is developed. The model incorporates changes in the point-spread function due to refractive index mismatch between the immersion ...
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ISBN:
(纸本)0819447641
An approximate model for optical-sectioning microscopy describing depth-varying imaging is developed. The model incorporates changes in the point-spread function due to refractive index mismatch between the immersion medium and the specimen. which causes spherical aberration that worsens with increasing depth tinder the coverslip. Comparison of model predictions to measured images from a bead phantom shows that the approximate model captures the main features in the data. The model presented in this paper is the first step towards depth-variant image estimation for optical-sectioning microscopy. An expectation maximization algorithm for maximum-likelihood restoration based on this model is also presented.
This paper describes a method for the improvement of biological images acquired using a Transmission Electronic Microscope (TEM). Several techniques are presented that deal with noise reduction, artifact removal and n...
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ISBN:
(纸本)0819427004
This paper describes a method for the improvement of biological images acquired using a Transmission Electronic Microscope (TEM). Several techniques are presented that deal with noise reduction, artifact removal and non-uniform illumination correction. Experimental results are shown.
Many high resolution optical methods are affected by the presence of optical aberrations. These include microscopy, three-dimensional optical data storage and optical micromachining. We investigate the use of adaptive...
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ISBN:
(纸本)0819461326
Many high resolution optical methods are affected by the presence of optical aberrations. These include microscopy, three-dimensional optical data storage and optical micromachining. We investigate the use of adaptive aberration correction applied to these techniques. In particular, it is shown how a deformable membrane mirror can be used to correct the aberrations when focusing deep into a multilayer optical data storage medium for both recording and read-out of data. Aberration correction for optical micromaching deep inside a substrate is also demonstrated.
In recent development of fluorescence microscopy, the out-of-focus fluorescence background that arises when imaging deep inside biological tissues is critical in determining the image quality and penetration depth. Fo...
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ISBN:
(纸本)9780819484413
In recent development of fluorescence microscopy, the out-of-focus fluorescence background that arises when imaging deep inside biological tissues is critical in determining the image quality and penetration depth. Focal modulation microscopy (FMM) is an advanced fluorescence technique that can provide high subcellular resolution when imaging thick specimens mainly by preserving the signal-to-background ratio.
We demonstrate a simple and light-efficient way of generating non-diffracting Bessel beams for use in confocal microscopy. A number of imaging modalities using such beams is discussed. Preliminary experimental results...
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ISBN:
(纸本)0819456756
We demonstrate a simple and light-efficient way of generating non-diffracting Bessel beams for use in confocal microscopy. A number of imaging modalities using such beams is discussed. Preliminary experimental results including brightfield. fluorescence and two-photon images are presented.
A snapshot high-sampling image Mapping Spectrometer (IMS) is developed for hyperspectral microscopy, measuring datacube of dimensions 285x285x60 (x,y,lambda). microscopy IMS is designed to work at the Nyquist sampling...
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ISBN:
(纸本)9780819479662
A snapshot high-sampling image Mapping Spectrometer (IMS) is developed for hyperspectral microscopy, measuring datacube of dimensions 285x285x60 (x,y,lambda). microscopy IMS is designed to work at the Nyquist sampling limit to realize high resolution imaging. The spatial resolution is similar to 0.45 mu m with FOV similar to 130 mu m. The spectral working range is 500-700nm with similar to 8.3nm average spectral resolution. Preliminary tests have been implemented on its imaging performances.
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