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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX"

共 592 条记录，以下是71-80 订阅

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Single image structured illumination (SISIM) for in-vivo imaging 25

Single image structured illumination (SISIM) for in-vivo ima...

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Conference on three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Hoffman, Zachary R. Kivanc, Kose Di Marzio, Charles A. Northeastern Univ 360 Huntington Ave Boston MA 02115 USA Mem Sloan Kettering Canc Ctr 1275 York Ave New York NY 10021 USA

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

Recently, it has been demonstrated that sectioning within a turbid media using structured illumination microscopy can be greatly enhanced through the use of a Hilbert transform algorithm. This technique, known as single image structured illumination microscopy (SISIM), provides high quality sectioning even within a strongly refracting/scattering medium, such as tissue. It is found that motion artifacts comprise a large portion of the error in in-vivo imaging. Thus, we expand on the SISIM technique by implementing an image registration algorithm which greatly mitigates motion induced artifacts. Multiple images and movies are developed to demonstrate SISIMs ability to provide real-time, in-vivo imaging of human tissue.

关键词： Structured Illumination optical sectioning thick specimen turbid media incoherent light image registration

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Evaluation of the use of wavefront encoding to reduce depth-induced aberration in structured illumination microscopy 25

Evaluation of the use of wavefront encoding to reduce depth-...

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Conference on three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Patwary, Nurmohammed Sola-Pikabea, Jorge Doblas, Ana Saavedra, Genaro Martinez-Corral, Manuel Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA Univ Valencia Dept Opt Burjassot Spain

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

three-dimensional imaging is affected by depth-induced spherical aberration (SA) when imaging deep into an optically thick sample. In this work, we evaluate the impact of SA on the performance of incoherent grating-projection structured illumination microscopy (SIM). In particular, we analyze the reduction of the contrast in the structured pattern and compare the reconstructed SIM images for different amounts of SA. In order to mitigate the impact of SA, we implement and evaluate in SIM a wavefront encoded imaging system using a square cubic (SQUBIC) phase mask, an approach shown previously to be successful in conventional microscopy.

关键词： Spherical aberration wavefront encoding fluorescence microscopy structured illumination microscopy

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Optical coherence computed tomography for quantitative 3D imaging of adult zebrafish (Withdrawal Notice) 25

Optical coherence computed tomography for quantitative 3D im...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXV 2018

作者： Van Der Horst, Jelle Trull, Anna Kalkman, Jeroen Technische Univ. Delft Netherlands

“Publisher’s Note: This conference presentation, originally published on 3/21/18, was withdrawn per author request.”

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614833

“Publisher’s Note: This conference presentation, originally published on 3/21/18, was withdrawn per author request.”

关键词： Tissue optics Coherence (optics) Computed tomography Optical coherence tomography Optical imaging Refractive index Signal attenuation 3D image processing Light scattering Optical tomography

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Theory and Applications of Structured Light Single Pixel Imaging 25

Theory and Applications of Structured Light Single Pixel Ima...

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Conference on three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Stokoe, Robert J. Stockton, Pateick A. Pezeshki, Ali Bartels, Randy A. Colorado State Univ Dept Elect & Comp Engn Ft Collins CO 80523 USA Colorado State Univ WM Keck Lab Raman Imaging Cell To Cell Commun Ft Collins CO 80523 USA Colorado State Univ Sch Biomed Engn Ft Collins CO 80523 USA

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

Many single-pixel imaging techniques have been developed in recent years. Though the methods of image acquisition vary considerably, the methods share unifying features that make general analysis possible. Furthermore, the methods developed thus far are based on intuitive processes that enable simple and physically-motivated reconstruction algorithms, however, this approach may not leverage the full potential of single-pixel imaging. We present a general theoretical framework of single-pixel imaging based on frame theory, which enables general, mathematically rigorous analysis. We apply our theoretical framework to existing single-pixel imaging techniques, as well as provide a foundation for developing more-advanced methods of image acquisition and reconstruction. The proposed frame theoretic framework for single-pixel imaging results in improved noise robustness, decrease in acquisition time, and can take advantage of special properties of the specimen under study. By building on this framework, new methods of imaging with a single element detector can be developed to realize the full potential associated with single-pixel imaging.

关键词： Fluorescence microscopy interference microscopy microscopy image formation theory optical transfer functions

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Low-cost light-sheet microscopy for whole brain imaging 25

Low-cost light-sheet microscopy for whole brain imaging

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Conference on three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Kumar, Manish Nasenbeny, Jordan Kozorovitskiy, Yevgenia Northwestern Univ Dept Neurobiol Evanston IL 60208 USA

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

Light-sheet microscopy has evolved as an indispensable tool in imaging biological samples. It can image 3D samples at fast speed, with high-resolution optical sectioning, and with reduced photobleaching effects. These properties make light-sheet microscopy ideal for imaging fluorophores in a variety of biological samples and organisms, e.g. zebrafish, drosophila, cleared mouse brains, etc. While most commercial turnkey light-sheet systems are expensive, the existing lower cost implementations, e.g. OpenSPIM, are focused on achieving high-resolution imaging of small samples or organisms like zebrafish. In this work, we substantially reduce the cost of light-sheet microscope system while targeting to image much larger samples, i.e. cleared mouse brains, at single-cell resolution. The expensive components of a light sheet system excitation laser, water-immersion objectives, and translation stage are replaced with an incoherent laser diode, dry objectives, and a custom-built Arduino-controlled translation stage. A low-cost CUBIC protocol is used to clear fixed mouse brain samples. The open-source platforms of Manager and Fiji support image acquisition, processing, and visualization. Our system can easily be extended to multi-color light-sheet microscopy.

关键词： Light-sheet microscopy Brain Fluorescence imaging Low-cost microscopy

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Fabrication and characterization of novel microsphere-embedded optical devices for enhancing microscopy resolution 25

Fabrication and characterization of novel microsphere-embedd...

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Conference on three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Darafsheh, Arash Washington Univ Sch Med Dept Radiat Oncol St Louis MO 63110 USA

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

Microsphere-assisted imaging can be incorporated onto conventional light microscopes allowing wide-field and flourescence imaging with enhanced resolution. We demonstrated that imaging of specimens containing sub diffraction-limited features is achievable through high-index microspheres embedded in a transparent thin film placed over the specimen. We fabricated novel microsphere-embedded microscope slides composed of barium titanate glass microspheres (with diameter 10-100 lam and refractive index-4.9-2.2) embedded in a transparent polydimethylsiloxane (PDMS) elastomer layer with controllable thickness. We characterized the imaging performance of such microsphere-embedded devices in white-light microscopies, by measuring the imaging resolution, field-of-view, and magnification as a function of microsphere size. Our results inform on the design of novel optical devices, such as microsphere-embedded microscope slides for imaging applications.

关键词： Super-resolution microscopy nanoscopy microsphere barium titanate PDMS

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Tradeoff between insensitivity to depth-induced spherical aberration and resolution of 3D fluorescence imaging due to the use of wavefront encoding with a radially symmetric phase mask 25

Tradeoff between insensitivity to depth-induced spherical ab...

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Conference on three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Doblas, Ana Dutta, Ananya Saavedra, Genaro Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA Univ Valencia Dept Opt E-46100 Burjassot Spain

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

Previously, a wavefront encoded (WFE) imaging system implemented using a squared cubic (SQUBIC) phase mask has been verified to reduce the sensitivity of the imaging system to spherical aberration (SA). The strength of the SQUBIC phase mask and, as consequence, the performance of the WFE system are controlled by a design parameter, A. Although the higher the A-value, the more tolerant the WFE system is to SA, this is accomplished at the expense of the effective imaging resolution. In this contribution, we investigate this tradeoff in order to find an optimal A-value to balance the effect of SA and loss of resolution.

关键词： three-dimensional microscopy wavefront encoding point-spread function engineering resolution limit

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Cross-Level Sampling for Bandwidth Effective Time Domain Fluorescence Lifetime Imaging Implementation (10499-48) 25

Cross-Level Sampling for Bandwidth Effective Time Domain Flu...

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Conference on three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Duran, Elvis Hoyos, Sebastian Jo, Javier Texas A&M Univ Dept Biomed Engn College Stn TX 77843 USA Texas A&M Univ Dept Elect Engn College Stn TX 77843 USA

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

The implementation of time domain fluorescence lifetime imaging (TD-FLIM) requires high cost bandwidth electronics and large storage capacity. A cross-level sampling technique for TD-FLIM is proposed. A simulation of this sampling technique was implemented using synthetic FLIM data. FLIM images were synthetically generated at different noise levels using a wide range of lifetimes. Each of the images was resampled using a cross-level approach and the lifetime maps were computed. Simulation results displayed strong correlation between the lifetime maps of the original and resampled images, suggesting that this sampling method could be adopted to reduce bandwidth and data transfer/ storage requirements.

关键词： Fluorescence lifetime imaging Luminescence Biomedical engineering Data acquisition Data analysis Data storage Deconvolution Electrical engineering Electronics Statistical analysis

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Spatiotemporal Polarization Modulation microscopy with a Microretarder Array 25

Spatiotemporal Polarization Modulation Microscopy with a Mic...

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Conference on three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Ding, Changqin Ulcickas, James R. W. Simpson, Garth J. Purdue Univ Dept Chem W Lafayette IN 47907 USA

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

A patterned microretarder array positioned in the rear conjugate plane of a microscope enables rapid polarization dependent nonlinear optical microscopy. The pattern introduced to the array results in periodic modulation of the polarization-state of the incident light as a function of position within the field of view with no moving parts or active control. Introduction of a single stationary optical element and a fixed polarizer into the beam of a nonlinear optical microscope enabled nonlinear optical tensor recovery, which informs on local structure and orientation. Excellent agreement was observed between the measured and predicted second harmonic generation (SHG) of z-cut quartz, selected as a test system with well-established nonlinear optical properties. Subsequent studies of spatially varying samples further support the general applicability of this relatively simple strategy for detailed polarization analysis in both conventional and nonlinear optical imaging of structurally diverse samples.

关键词： Second harmonic generation two-photon excited fluorescence microscopy pharmaceutical analysis

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Light-sheet enhanced resolution of light field microscopy for rapid imaging of large volumes 25

Light-sheet enhanced resolution of light field microscopy fo...

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Conference on three-dimensional and multidimensional microscopy: image acquisition and processing XXV

作者： Madrid-Wolff, Jorge Castro, Diego Arbelaez, Pablo Forero-Shelton, Manu Univ Los Andes Biophys Lab Bogota Colombia Univ Los Andes Biomed Comp Vis Grp Bogota Colombia

ISBN: (数字)9781510614840

ISBN: (纸本)9781510614840;9781510614833

Whole-brain imaging is challenging because it demands microscopes with high temporal and spatial resolution, which are often at odds, especially in the context of large fields of view. We have designed and built a light-sheet microscope with digital micromirror illumination and light-field detection. On the one hand, light sheets provide high resolution optical sectioning on live samples without compromising their viability. On the other hand, light field imaging makes it possible to reconstruct full volumes of relatively large fields of view from a single camera exposure;however, its enhanced temporal resolution comes at the expense of spatial resolution, limiting its applicability. We present an approach to increase the resolution of light field images using DMD-based light sheet illumination. To that end, we develop a method to produce synthetic resolution targets for light field microscopy and a procedure to correct the depth at which planes are refocused with rendering software. We measured the axial resolution as a function of depth and show a three-fold potential improvement with structured illumination, albeit by sacrificing some temporal resolution, also three-fold. This results in an imaging system that may be adjusted to specific needs without having to reassemble and realign it. This approach could be used to image relatively large samples at high rates.

关键词： Light field microscopy light sheet fluorescence microscopy resolution enhancement structured illumination Digital Micromirror Device

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