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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Lai, XM Ward, ES Lin, ZP Ober, RJ Univ Texas SW Med Ctr Ctr Immunol Dallas TX 75235 USA

ISBN: (纸本)0819456756

A recently developed algorithm is applied to calculate a state space realization of a 3D microscopy image set. It is based on interpreting the image set as the impulse response of a 3D se arable system. As an application it is shown how this algorithm. combined with approximation steps, can be used to suppress noise in 3D experimental point spread functions. The approach was motivated by a well known problem that a noisy point spread function degrades the results of deconvolution algorithms for the restoration of 3D fluorescence microscopy image sets. The proposed approach can also be applied to 3D fluorescence microscopy image sets of cells.

关键词： point spread function estimation state space algorithm noise suppression

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Lateral resolution enhancement in confocal self-interference microscopy

Lateral resolution enhancement in confocal self-interference...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Kang, D Gweon, D Korea Adv Inst Sci & Technol Med Engn Dept NOM Lab Taejon 305701 South Korea

ISBN: (纸本)0819456756

Lateral resolution enhancement in confocal self-interference microscopy (CSIM) is evaluated. CSIM, which uses the birefringence of the calcite plate to generate self-interference pattern, sharpens the central lobe of the effective spot. Numerical simulation results of two-dimensional imaging performances are presented. Two-point resolution of 149nm is achieved, which is enhanced by nearly 100% compared to that of confocal microscopy.

关键词： confocal microscopy superresolution birefringence self-interference

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Fluorescence microscopy with isotropic resolution using three objectives 13

Fluorescence microscopy with isotropic resolution using thre...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Huelsnitz, Thomas Kner, Peter Univ Georgia Coll Engn Athens GA 30602 USA

ISBN: (纸本)9781628419474

Widefield and confocal fluorescence microscopy using a single objective suffer from poor resolution and a strong anisotropy between the lateral and axial resolution. Coherently combining the excitation and emission from two coaxial objectives improves the axial resolution up to sevenfold, but leaves the lateral resolution unchanged. Here we investigate the coherent combination of three objectives to create a point spread function (PSF) that is isotropic with higher resolution in the plane of the objectives. We develop a theoretical framework for simulating the performance of interferometric imaging with three objectives. Using three identical objectives with a large working distance and 0.9 numerical aperture (NA), the full-width half maximum of the confocal PSF is 135 nm compared to the lateral FWHM of 274 nm for imaging with a single objective at a wavelength of 515 nm.

关键词： microscopy fluorescence microscopy interferometric imaging PSF Engineering

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three-dimensional measurement of microorganism by retardation modulated differential interference contrast microscope

Three-dimensional measurement of microorganism by retardatio...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XIII

作者： Aoki, G. Itoh, M. Yasuno, Y. Yatagai, T. Univ Tsukuba Inst Appl Phys 1-1-1 Tennodai Tsukuba Ibaraki 3958573 Japan

ISBN: (纸本)0819461326

We propose a new technique for obtaining three-dimensional phase distribution on differential interference contrast microscope to modulate relative phase retardation between two shear beams. Using partial coherent theory we extract the phase information from two different retardation images. For the object in a weak phase region, simple formula is derived. The images of nematomorph were obtained in vivo.

关键词： differential interference contrast retardation modulated phase measurement microscope Nomarski

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Deformable mirror based remote focusing for fast three-dimensional microscopy 13

Deformable mirror based remote focusing for fast three-dimen...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIII

作者： Zurauskas, Mantas Frade, Maria Booth, Martin Univ Oxford Ctr Neural Circuits & Behav Mansfield Rd Oxford OX1 3SR England Univ Oxford Dept Engn Sci Parks Rd Oxford OX1 3PJ England

ISBN: (纸本)9781628419474

We present a deformable mirror based remote focusing method for three-dimensional imaging in high-resolution microscopy systems. The method relies on predefined mirror mode arrays that are obtained during initial mirror training step with a low complexity wavefront-sensing module. The imaging plane can be refocused over distances over a hundred times greater than the original depth of field of the objective lens along the optical axis at millisecond rates. We will demonstrate the combination of the remote focusing method with spatiotemporally focused two-photon excitation applied to three-dimensional imaging of biological samples.

关键词： Remote focusing adaptive optics three dimensional microscopy two photon microscopy

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Inverse scattering with subwavelength resolution in the near-zone

Inverse scattering with subwavelength resolution in the near...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing VIII

作者： Carney, PS Schotland, JC Washington Univ Dept Elect Engn St Louis MO 63130 USA

ISBN: (纸本)0819439398

In classical microscopy and inverse scattering, images are made from far field measurements. It will be shown that the standard Abbe or Rayleigh resolution limits arise as a result of assumptions which do not hold in the near field. Existing technologies such as TIRM and PSTM that exploit these properties of the near field produce two dimensional images which exhibit structure on a subwavelength scale. These images have proven difficult to interpret when the sample presents variations in structure and optical properties simultaneously in three dimensions. We present the analytic singular value decomposition of the linearized scattering operator. This representation leads to a robust inversion algorithm for the inverse scattering problem in the near zone. Numerical implementation of the algorithm is demonstrated using simulated noisy data to produce tomographic images of a three dimensional scatterer.

关键词： near-field optics inverse scattering microscopy tomography

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Inhomogeneous polarization in scanning optical microscopy

Inhomogeneous polarization in scanning optical microscopy

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Conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Youngworth, KS Brown, TG Univ of Rochester Rochester United States

ISBN: (纸本)081943535X

Polarization control and analysis have proven to be a powerful tool both in conventional and confocal microscopies, but most of these approaches have used homogeneously polarized pupils. Other groups have had varying levels of success producing beams with spatially inhomogeneous polarization. In this paper, we describe the generation and focusing properties of such beams and their application to scanning laser microscopy. We specifically consider microscopy using the lowest-order azimuthally polarized beam.

关键词： spatially inhomogeneous polarization scanning optical microscopy azimuthal polarization radial polarization cylindrical-vector beam polarization converter

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three-dimensional microscopy through white light interferometry

Three-dimensional microscopy through white light interferome...

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Conference on 3-dimensional and multidimensional microscopy: image acquisition and processing IX

作者： Laude, B De Martino, A Drévillon, B Benattar, L Schwartz, L Ecole Polytech CNRS UMR 7647 Lab Phys Interfaces & Couches Minces F-91128 Palaiseau France

ISBN: (纸本)0819443603

A very simple OCT system has been developed, based on a Linnik interferometric microscope with its reference mirror mounted on a piezoelectric translator. The geometrical extension of the optics allows efficient illumination of this device with a low power (3 W) light bulb, yielding full field interferometric images at 50 Hz acquisition rate with a fast CCD camera. Due to the very broad spectral width of the light source and camera response, a longitudinal resolution of 1.5 mum is achieved. Tomographic images of cell smears are shown.

关键词： Optical Coherence Tomography microscopy biomedical optical instrumentation

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Coherent light 3D materials microscopy without phase retrieval 24

Coherent light 3D materials microscopy without phase retriev...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIV

作者： Xing, Jian Yu, Jiun-Yann Chen, Simeng Cogswell, Carol Univ Colorado Dept Elect Comp & Energy Engn 425 UCB Boulder CO 80309 USA

ISBN: (数字)9781510605824

ISBN: (纸本)9781510605817;9781510605824

We report a novel microscopy technique, utilizing our previously reported expanded point information content (EPIC) concept [1], to extend the technique into the coherent regime. Preliminary data shows coherent EPIC (CoEPIC) can image reflective samples successfully, and can recover the 3D structure without the need to acquire an image stack at multiple depths. Numerical simulation demonstrates the potential super-resolution capability of CoEPIC.

关键词： 3D materials inspection PSF engineering phase retrieval super-resolution coherent illumination

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Developments and applications of confocal theta microscopy

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Proceedings of SPIE - The International Society for Optical Engineering 1999年 3605卷 48-54页

作者： Haar, Frank-Martin Swoger, Jim Stelzer, Ernst H.K. Heidelberg Germany

ISBN: (纸本)0819430757;9780819430755

Confocal theta microscopy improves the resolution of confocal laser scanning microscopes by instrumentally solving the problem of the inferior axial resolution. A technical variation of this microscopy technique, the Single-Lens Theta microscopy (SLTM), is designed to be easily adapted to any common confocal laser scanning microscope. With SLTM, different kinds of other microscopical techniques are possible.

关键词： Optical microscopy

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