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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Juskaitis, R Botcherby, EJ Wilson, T Univ Oxford Dept Engn Sci Oxford England

We demonstrate a simple and light-efficient way of generating non-diffracting Bessel beams for use in confocal microscopy. A number of imaging modalities using such beams is discussed. Preliminary experimental results... 详细信息

ISBN: (纸本)0819456756

关键词： confocal extended depth of focus Bessel beam fluorescence microscopy polarisation multi-photon

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image estimation accounting for point-spread function depth variation in three-dimensional fluorescence microscopy

Image estimation accounting for point-spread function depth ...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing X

作者： Preza, C Conchello, JA Washington Univ Dept Elect Engn St Louis MO 63130 USA

ISBN: (纸本)0819447641

An approximate model for optical-sectioning microscopy describing depth-varying imaging is developed. The model incorporates changes in the point-spread function due to refractive index mismatch between the immersion medium and the specimen. which causes spherical aberration that worsens with increasing depth tinder the coverslip. Comparison of model predictions to measured images from a bead phantom shows that the approximate model captures the main features in the data. The model presented in this paper is the first step towards depth-variant image estimation for optical-sectioning microscopy. An expectation maximization algorithm for maximum-likelihood restoration based on this model is also presented.

关键词： 3D microscopy depth-variant imaging model maximum likelihood estimation

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three-dimensional reconstruction and analysis of gastric malignancies by electronic slides of consecutive sections and virtual microscopy

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Proceedings of SPIE - The International Society for Optical Engineering 1999年 3605卷 190-199页

作者： Molnar, Bela Papik, Kornel Tagscherer, Attila Csendes, Gabor Varga, Sebestyen V. Tulassay, Zsolt Schaefer, Rainer Mahoney, Walt Semmelweis Univ Budapest Hungary

ISBN: (纸本)0819430757;9780819430755

A study was carried out focusing on the application of an electronic slide and a software simulated virtual microscope to histological analysis. The electronic slide was developed using a fully computer controlled microscope, video camera and an Intel Pentium II based PC. A program called Pyramid was developed for the scanning of an entire slide. Autofocusing, image reduction and frame joining algorithms were implemented in the virtual microscope application. Results confirm that the electronic slide and the software contribute to a more efficient, convenient histological analysis.

关键词： Optical microscopy

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Lateral resolution enhancement in confocal self-interference microscopy

Lateral resolution enhancement in confocal self-interference...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Kang, D Gweon, D Korea Adv Inst Sci & Technol Med Engn Dept NOM Lab Taejon 305701 South Korea

ISBN: (纸本)0819456756

Lateral resolution enhancement in confocal self-interference microscopy (CSIM) is evaluated. CSIM, which uses the birefringence of the calcite plate to generate self-interference pattern, sharpens the central lobe of the effective spot. Numerical simulation results of two-dimensional imaging performances are presented. Two-point resolution of 149nm is achieved, which is enhanced by nearly 100% compared to that of confocal microscopy.

关键词： confocal microscopy superresolution birefringence self-interference

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3D deconvolution of adaptive-optics corrected retinal images

3D deconvolution of adaptive-optics corrected retinal images

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XIII

作者： Chenegros, G. Mugnier, L. M. Lacombe, F. Glanc, M. Off Natl Etud & Rech Aerosp Dept Opt BP 72 F-92322 Chatillon France Mauna Kea Technol F-75010 Paris France Lab Etudes Spatiales Instrument Astrophys Paris France

ISBN: (纸本)0819461326

We report on a deconvolution method developed in a Bayesian framework for adaptive-optics corrected images of the human retina. The method takes into account the three-dimensional nature of the imaging process;it incorporates a positivity constraint and a regularization metric in order to avoid uncontrolled noise amplification. This regularization metric is designed to simultaneously smooth noise out and preserve edges, while staying convex in order to keep the solution unique. We demonstrate the effectiveness of the method, and in particular of the edge-preserving regularization, on realistic simulated data.

关键词： image restoration deconvolution three-dimensional imaging adaptive optics retinal imaging microscopy

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three-dimensional microscopy through white light interferometry

Three-dimensional microscopy through white light interferome...

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Conference on 3-dimensional and multidimensional microscopy: image acquisition and processing IX

作者： Laude, B De Martino, A Drévillon, B Benattar, L Schwartz, L Ecole Polytech CNRS UMR 7647 Lab Phys Interfaces & Couches Minces F-91128 Palaiseau France

ISBN: (纸本)0819443603

A very simple OCT system has been developed, based on a Linnik interferometric microscope with its reference mirror mounted on a piezoelectric translator. The geometrical extension of the optics allows efficient illumination of this device with a low power (3 W) light bulb, yielding full field interferometric images at 50 Hz acquisition rate with a fast CCD camera. Due to the very broad spectral width of the light source and camera response, a longitudinal resolution of 1.5 mum is achieved. Tomographic images of cell smears are shown.

关键词： Optical Coherence Tomography microscopy biomedical optical instrumentation

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Coherent light 3D materials microscopy without phase retrieval 24

Coherent light 3D materials microscopy without phase retriev...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXIV

作者： Xing, Jian Yu, Jiun-Yann Chen, Simeng Cogswell, Carol Univ Colorado Dept Elect Comp & Energy Engn 425 UCB Boulder CO 80309 USA

ISBN: (数字)9781510605824

ISBN: (纸本)9781510605817;9781510605824

We report a novel microscopy technique, utilizing our previously reported expanded point information content (EPIC) concept [1], to extend the technique into the coherent regime. Preliminary data shows coherent EPIC (CoEPIC) can image reflective samples successfully, and can recover the 3D structure without the need to acquire an image stack at multiple depths. Numerical simulation demonstrates the potential super-resolution capability of CoEPIC.

关键词： 3D materials inspection PSF engineering phase retrieval super-resolution coherent illumination

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Adaptive optics for three-dimensional optical data storage and micromachining

Adaptive optics for three-dimensional optical data storage a...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XIII

作者： Booth, Martin J. Schwertner, Michael Wilson, Tony Univ Oxford Dept Engn Sci Parks Rd Oxford OX1 3PJ England

ISBN: (纸本)0819461326

Many high resolution optical methods are affected by the presence of optical aberrations. These include microscopy, three-dimensional optical data storage and optical micromachining. We investigate the use of adaptive aberration correction applied to these techniques. In particular, it is shown how a deformable membrane mirror can be used to correct the aberrations when focusing deep into a multilayer optical data storage medium for both recording and read-out of data. Aberration correction for optical micromaching deep inside a substrate is also demonstrated.

关键词： Aberration correction Optical storage Monochromatic aberrations Adaptive optics Micromachining Microscopes 3D optical data storage Confocal microscopy Data storage Objectives

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Automatic quantification of neurite outgrowth by means of image analysis

Automatic quantification of neurite outgrowth by means of im...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Van de Wouwer, G Nuydens, R Meert, T Weyn, B Univ Antwerp Visielab Dept Phys B-2020 Antwerp Belgium

ISBN: (纸本)0819452327

A system for quantification of neurite outgrowth in in-vitro experiments is described. The system is developed for routine use in a high-throughput setting and is therefore needs fast, cheap, and robust. It relies on automated digital microscopical imaging of microtiter plates. image analysis is applied to extract features for characterisation of neurite outgrowth. The system is tested in a dose-response experiment on PC12 cells + Taxol. The performance of the system and its ability to measure changes on neuronal morphology is studied.

关键词： automation microscopy image analysis neurite outgrowth

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three-dimensional state space realization algorithm: noise suppression of fluorescence microscopy images and point spread functions

Three-dimensional state space realization algorithm: noise s...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Lai, XM Ward, ES Lin, ZP Ober, RJ Univ Texas SW Med Ctr Ctr Immunol Dallas TX 75235 USA

ISBN: (纸本)0819456756

A recently developed algorithm is applied to calculate a state space realization of a 3D microscopy image set. It is based on interpreting the image set as the impulse response of a 3D se arable system. As an application it is shown how this algorithm. combined with approximation steps, can be used to suppress noise in 3D experimental point spread functions. The approach was motivated by a well known problem that a noisy point spread function degrades the results of deconvolution algorithms for the restoration of 3D fluorescence microscopy image sets. The proposed approach can also be applied to 3D fluorescence microscopy image sets of cells.

关键词： point spread function estimation state space algorithm noise suppression

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