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three-dimensional and multidimensional microscopy: image acquisition and processing XVIII

Three-Dimensional and Multidimensional Microscopy: Image Acq...

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three-dimensional and multidimensional microscopy: image acquisition and processing XVIII

ISBN: (纸本)9780819484413

The proceedings contain 42 papers. The topics discussed include: lens-less holographic microscope with high resolving power and no-distortion;in-line digital holographic microscopy based on intensity measurements at two planes;simplified setup for imaging with digital holographic microscopy and enhanced quantitative phase contrast by osmotic stimulation of living cells;whole cell imaging based on wide-field interferometric phase microscopy and its application to cardiomyocytes;spatial-spectral 3D imaging system using broadband sources;development of a digital holographic microscopy system integrated with atomic force microscope;3D optical trapping calibration and optical micromanipulation using 808-nm diode-laser bar;heart synchronization for SPIM microscopy of living zebra fish;and high-speed focal modulation microscopy using acousto-optical modulators for visualization of thick biological specimens.

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three-dimensional data acquisition with aberrations correction for video-rate microscopy

Three-dimensional data acquisition with aberrations correcti...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Samim, Masood Cisek, Richard Sandkuijl, Daaf Tretyakov, Ian Siu, Sam Musikhin, Sergey Barzda, Virginijus Univ Toronto Dept Chem & Phys Sci Mississauga ON L5L 1C6 Canada

ISBN: (纸本)9780819484413

We demonstrate a multimodal, multifocal, differential nonlinear optical microscope, which is equipped with a pair of deformable mirrors and a Shack-Hartmann sensor for dynamic wavefront manipulation. The optical wavefronts of a home built Yb:KGW femtosecond (1028 nm) laser-beams are engineered to perform multidepth focusing in differential mode with simultaneous corrections for optical aberrations. The 39-actuator deformable mirrors provide fast reshaping of the wavefront and optical aberrations correction of the diffraction-limited focal volume allowing for fast axial scanning. Combination of similar to 200 frames per second lateral scanning with fast refocusing enables a three-dimensional video rate scanning capability, which is essential for studying rapid dynamics in biological organisms, such as blood flow, cardiac contractions, and motility of microorganisms in a three-dimensional volume.

关键词： Deformable mirrors adaptive optics nonlinear optical microscopy harmonic generation microscopy non-invasive in vivo imaging three-dimensional imaging multi-contrast microscopy

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High-speed focal modulation microscopy using acousto-optical modulators for visualization of thick biological specimens

High-speed focal modulation microscopy using acousto-optical...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Chong, Shau Poh Gao, Guangjun Sheppard, Colin J. R. Chen, Nanguang Natl Univ Singapore Div Bioengn Singapore 117576 Singapore

ISBN: (纸本)9780819484413

In recent development of fluorescence microscopy, the out-of-focus fluorescence background that arises when imaging deep inside biological tissues is critical in determining the image quality and penetration depth. Focal modulation microscopy (FMM) is an advanced fluorescence technique that can provide high subcellular resolution when imaging thick specimens mainly by preserving the signal-to-background ratio.

关键词： Fluorescence microscopy multiple scattering penetration depth

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In-line digital holographic microscopy based on intensity measurements at two planes

In-line digital holographic microscopy based on intensity me...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Das, Bhargab Yelleswarapu, Chandra S. Rao, D. V. G. L. N. Univ Massachusetts Dept Phys Boston MA 02125 USA

ISBN: (纸本)9780819484413

We propose a new approach of in-line digital holographic microscopy (DHM) with the capability of enhancing the hologram acquisition rate together with improved reconstruction capability. The method is based on the recording of two interferograms of the same object at slightly different planes. The technique utilizes the full spatial bandwidth of the camera and do not require phase-shifting of the reference beam. Furthermore, we exploit the method of subtraction of average intensity of the entire hologram to suppress the zero-order diffracted wave. The twin image is eliminated by Fourier domain processing of the two recorded holograms. Experimental results of both amplitude and phase objects demonstrate the feasibility of this method. Since the two interferograms can be recorded simultaneously by using two CCD or CMOS sensors, this new in-line DHM technique has the potential applications in biomedical research for the visualization of rapid dynamic processes at cellular level.

关键词： Digital holography microscopy Numerical Reconstruction Quantitative Phase Imaging

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Heart Synchronization for SPIM microscopy of Living Zebra Fish

Heart Synchronization for SPIM Microscopy of Living Zebra Fi...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Taylor, J. M. Saunter, C. D. Chaudhry, B. Henderson, D. J. Love, G. D. Girkin, J. M. Univ Durham Dept Phys Ctr Adv Instrumentat South Rd Durham DH1 3LE England Newcastle Univ Inst Human Genet Newcastle Upon Tyne NE1 3BZ Tyne & Wear England

We describe work on producing a selective plane illumination microscope for cardiac imaging in zebra fish embryos. The system has a novel synchronization system for imaging oscillating structures (e.g. the heart) and ... 详细信息

ISBN: (纸本)9780819484413

关键词： Optical microscopy Selective Plane Illumination Synchronization

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Time-resolved confocal microscopy of cryogenic processes in biological tissues

Time-resolved confocal microscopy of cryogenic processes in ...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Schellenberg, M. Bergmann, T. Peev, E. Kloster, M. Napier, J. Neu, W. Univ Appl Sci Emden Leer Dept Nat Sci Constantiapl 4 D-26723 Emden Germany Univ Appl Sci Giessen Friedberg Inst Medizin Phys & Strahlenschutz D-35390 Giessen Germany

ISBN: (纸本)9780819484413

Cryogenic procedures are fundamental tools in modern biology, e. g. for conservation or purification of biological materials. The processes occurring in biological cells and tissues during freezing and thawing are subject to ongoing research. Optimization of cell survival rates demands the development and evaluation of exactly defined temperature profiles. 4D-DMD-microscopy is capable of imaging these highly dynamic processes with high spatial and temporal resolution, utilizing well established staining procedures for differentiating structures of interest.

关键词： two-photon DMD confocal microscopy cryogenic process 4D-microscopy cryomicroscopy

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Adaptive Optics for Wide-field microscopy

Adaptive Optics for Wide-field Microscopy

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Bourgenot, C. Saunter, C. D. Girkin, J. M. Love, G. D. Univ Durham Dept Phys Ctr Adv Instrumentat Durham DH1 3LE England

ISBN: (纸本)9780819484413

We report on recent developments in the use of adaptive optics (AO) in wide-field microscopy to remove both system and sample induced aberrations. We describe progress on using both a full AO system and image optimization techniques (wavefront sensorless AO). In the latter system the determination of the best mirror shape is found via two routes. In the first an optimization algorithm using a Simplex search pattern is used with an initial random set of mirror shapes. We then explore the use of specific Zernike terms as our starting basis set. In both cases the final optimization performance is not affected by the choice of optimization metric. We then describe an open loop AO system in which the equivalent of a laser guide star is used as the light source for the wavefront sensor.

关键词： Optical microscopy Aberration correction Adaptive Optics

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Optically Sectioned Imaging by Oblique Plane microscopy

Optically Sectioned Imaging by Oblique Plane Microscopy

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Kumar, Sunil Lin, Ziduo Lyon, Alex R. MacLeod, Ken T. Dunsby, Chris Univ London Imperial Coll Sci Technol & Med Dept Phys Photon Grp S Kensington Campus London SW7 2AZ England Imperial Coll London Natl Heart & Lung Inst London W2 1NY England Imperial Coll London Natl Heart & Lung Inst London SW3 6LY England Imperial Coll London Dept Med Div Med London W12 0HS England

ISBN: (纸本)9780819484413

Oblique Plane microscopy (OPM) is a light sheet microscopy technique that combines oblique illumination with correction optics that tilt the focal plane of the collection system. OPM can be used to image conventionally mounted specimens on coverslips or tissue culture dishes and has low out-of-plane photobleaching and phototoxicity. No moving parts are required to achieve an optically sectioned image and so high speed optically sectioned imaging is possible. The first OPM results obtained using a high NA water immersion lens on a commercially available inverted microscope frame are presented, together with a measurement of the achievable optical resolution.

关键词： Fluorescence microscopy three-dimensional microscopy Selective Plane Illumination microscopy

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Fourier Analysis of Mitochondrial Distribution in Oocytes

Fourier Analysis of Mitochondrial Distribution in Oocytes

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Hollmann, Joseph L. Brooks, Dana H. Newmark, Judith A. Warner, Carol M. DiMarzio, Charles A. Northeastern Univ Dept Elect & Comp Engn Dana Res Ctr 409 360 Huntington Ave Boston MA 02115 USA Northeastern Univ Dept Biol Mugar Life Sci 134 Boston MA 02115 USA Compucyte Boston MA 02115 USA Northeastern Univ Dept Mech & Ind Engn Boston MA 02115 USA

ISBN: (纸本)9780819484413

This paper describes a novel approach to quantifying mitochondrial patterns which are typically described using the qualitative terms "diffuse" "aggregated" and are potentially key indicators for an oocyte's health and survival potential post-implantation. An oocyte was isolated in a confocal image and a coarse grid was superimposed upon it. The spatial spectrum was calculated and an aggregation factor was generated. A classifier for healthy cells was developed and verified. The aggregation factor showed a clear distinction between the healthy and unhealthy oocytes. The ultimate goal is to screen oocytes for viability preimplantation, thus improving the outcome of in vitro fertilization (IVF) treatments.

关键词： Spatial frequencies image analysis Confocal microscopy 3D image processing Algorithm development MATLAB Digital image correlation Microscopes microscopy Visualization

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Reducing noise in extended depth of field microscope images by optical manipulation of the point spread function

Reducing noise in extended depth of field microscope images ...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Zahreddine, Ramzi N. Cormack, Robert H. Cogswell, Carol J. Univ Colorado Dept Elect Comp & Energy Engn Boulder CO 80309 USA

ISBN: (纸本)9780819484413

This work describes improved methods and algorithms for implementing extended depth of field (EDF) microscopy through point spread function (PSF) engineering. It utilizes adaptive optics to create a test bed on which to evaluate new phase shapes for EDF. Being able to quickly and cheaply design novel PSFs is essential to overcome limitations of EDF that have prevented the technology from reaching mainstream use. Further improvement is made by reducing the noise normally seen in EDF images. Computational optics principles are used to first encode the noise with an identifiable pattern and a specially-tailored non-linear algorithm then removes the noise. This approach improves a microscope's imaging capabilities in photon-starved applications such as live-cell fluorescence and object tracking.

关键词： Extended Depth of Field PSF Engineering 3D microscopy Adaptive Optics Neural Networks Noise Removal

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