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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII"

共 590 条记录，以下是281-290 订阅

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Semi-automated Algorithm for Localization of Dermal/Epidermal Junction in Reflectance Confocal microscopy images of Human Skin

Semi-automated Algorithm for Localization of Dermal/Epiderma...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVIII

作者： Kurugol, Sila Dy, Jennifer G. Rajadhyaksha, Milind Gossage, Kirk W. Weissman, Jesse Brooks, Dana H. Northeastern Univ 360 Huntington Av Boston MA 02115 USA Mem Sloan Kettering Canc Ctr Serv Dermatol New York NY 10065 USA Unilever Res Labs Trumbull CT 06611 USA

ISBN: (纸本)9780819484413

The examination of the dermis/epidermis junction (DEJ) is clinically important for skin cancer diagnosis. Reflectance confocal microscopy (RCM) is an emerging tool for detection of skin cancers in vivo. However, visual localization of the DEJ in RCM images, with high accuracy and repeatability, is challenging, especially in fair skin, due to low contrast, heterogeneous structure and high inter-and intra-subject variability. We recently proposed a semi-automated algorithm to localize the DEJ in z-stacks of RCM images of fair skin, based on feature segmentation and classification. Here we extend the algorithm to dark skin. The extended algorithm first decides the skin type and then applies the appropriate DEJ localization method. In dark skin, strong backscatter from the pigment melanin causes the basal cells above the DEJ to appear with high contrast. To locate those high contrast regions, the algorithm operates on small tiles (regions) and finds the peaks of the smoothed average intensity depth profile of each tile. However, for some tiles, due to heterogeneity, multiple peaks in the depth profile exist and the strongest peak might not be the basal layer peak. To select the correct peak, basal cells are represented with a vector of texture features. The peak with most similar features to this feature vector is selected. The results show that the algorithm detected the skin types correctly for all 17 stacks tested (8 fair, 9 dark). The DEJ detection algorithm achieved an average distance from the ground truth DEJ surface of around 4.7 mu m for dark skin and around 7-14 mu m for fair skin.

关键词： confocal reflectance microscopy image analysis skin classification

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three-dimensional and multidimensional microscopy: image acquisition and processing XVII

Three-Dimensional and Multidimensional Microscopy: Image Acq...

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three-dimensional and multidimensional microscopy: image acquisition and processing XVII

ISBN: (纸本)9780819479662

The proceedings contain 36 papers. The topics discussed include: quantitative depth-variant imaging for fluorescence microscopy using the COSMOS software package;comparison of estimation algorithms in single-molecule localization;closed loop adaptive optics for microscopy without a wavefront sensor;Gabor wavelet transform for dynamic analysis in digital holographic microscopy;a wide field-of-view microscope based on holographic focus grid;dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity;quadriwave lateral shearing interferometry for quantitative phase microscopy: applications to long-duration imaging;dual-modal optical projection tomography microscopy for cancer diagnosis;and integrated optical coherence tomography and optical coherence microscopy imaging of human pathology.

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Optical design of a snapshot high-sampling image Mapping Spectrometer (IMS) for hyperspectral microscopy

Optical design of a snapshot high-sampling Image Mapping Spe...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Gao, Liang S. Kester, Robert T. Tkaczyk, Tomasz S. Rice Univ Dept Bioengn Houston TX 77005 USA

ISBN: (纸本)9780819479662

A snapshot high-sampling image Mapping Spectrometer (IMS) is developed for hyperspectral microscopy, measuring datacube of dimensions 285x285x60 (x,y,lambda). microscopy IMS is designed to work at the Nyquist sampling limit to realize high resolution imaging. The spatial resolution is similar to 0.45 mu m with FOV similar to 130 mu m. The spectral working range is 500-700nm with similar to 8.3nm average spectral resolution. Preliminary tests have been implemented on its imaging performances.

关键词： Hyperspectral microscopy image Mapping Spectrometer

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Dual-modal optical projection tomography microscopy for cancer diagnosis

Dual-modal optical projection tomography microscopy for canc...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Miao, Qin Yu, Julia Meyer, Michael G. Rahn, J. Richard Neumann, Thomas Nelson, Alan C. Seibel, Eric J. Univ Washington Dept Bioengn Seattle WA 98195 USA VisGate Inc Gig Harbor WA 98335 USA Univ Washington Dept Engn Mech Seattle WA 98195 USA

ISBN: (纸本)9780819479662

A dual-modal optical projection tomography microscope (OPTM) is presented, which produces three-dimensional images of single cells with isometric high resolution both in fluorescence and absorption mode. Depth of field of a high numerical aperture objective is extended by scanning the focal plane through the sample in order to enable reconstruction by back-projection method. Cells are fixed, stained, and mixed with optical gel and injected into the capillary for imaging. Combining absorption and fluorescence mode allows us to image different aspects of the disease process. images of cells stained with both hematoxylin and fluorescence probes are shown. Registrations between two modes are discussed.

关键词： three-dimensional microscopy Tomographic imaging Fluorescence biomarker imaging Coregistration

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Pupil Polarimetry using Stress-Engineered Optical Elements

Pupil Polarimetry using Stress-Engineered Optical Elements

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Beckley, Amber M. Brown, Thomas G. Univ Rochester Inst Opt Rochester NY 14627 USA

ISBN: (纸本)9780819479662

Stress-engineered optical elements show fascinating and potentially useful effects when placed at the pupil plane of an imaging system. When illuminated by a beam of spatially uniform polarization, a snapshot (single measurement) polarimetry method can be constructed. We expand upon this method to perform snapshot pupil polarimetry for spatially varying pupil polarization. We present the theory for snapshot non-uniform pupil polarization measurement using a stress-engineered optical element, as well as simulation results.

关键词： Polarimetry microscopy Cylindrical Vector Beams

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Gabor wavelet transform for dynamic analysis in digital holographic microscopy

Gabor wavelet transform for dynamic analysis in digital holo...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Zhong, Jingang Weng, Jiawen Hu, Cuiying Jinan Univ Inst Optoelect Engn Guangzhou 510632 Guangdong Peoples R China

ISBN: (纸本)9780819479662

Real time dynamic analysis of micro-object is one of the significant advantages of digital holographic microscopy, but the process of the spatial filtering limits the application of dynamic and automatic analysis in digital holographic microscopy. In this paper, a numerical reconstruction technique by means of Gabor wavelet transform (GWT) is presented for real time dynamic analysis. Appling the GWT to the hologram, the quantitative phase information of the micro-object is obtained automatically without the process of the spatial filtering. An experiment with a sequence of holograms of an animalcule is proposed for the dynamic and automatic analysis by employing the GWT method.

关键词： Digital holographic microscopy dynamic analysis wavelet transform full-field quantitative phase imaging

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Extended depth-of-field microscopy

Extended depth-of-field microscopy

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Smith, Christopher Botcherby, Edward Booth, Martin Juskaitis, Rimas Wilson, Tony Univ Oxford Dept Engn Sci Oxford OX1 3PJ England

ISBN: (纸本)9780819479662

In this paper, we present details of a scanning two-photon fluorescence microscope we have built with a near-isotropic scan rate. This means that the focal spot can be scanned at high speed along any direction in the specimen, without introducing systematic aberrations. We present experimental point spread function measurements for this system using an Olympus 1.4 NA 60X oil immersion lens that demonstrates an axial range of operation greater than 70 mu m. We give details of a novel actuator device used to displace the focusing element and demonstrate axial scan responses up to 3.5 kHz. Finally, we present an application of this system in liquid crystal research to image the dynamic response of a nematic device during switching. Information about the director field at different levels in the device can be inferred from images acquired with a temporal resolution of 2.5 ms.

关键词： optical refocusing two-photon microscopy

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Fully Automated Data acquisition and Fast Data Interpretation in a Customized Multimodal Multiphoton Microscope

Fully Automated Data Acquisition and Fast Data Interpretatio...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Rahn, C. D. Meine, H. Gallinat, S. Wenck, H. Wittern, K. P. Fischer, F. Beiersdorf AG R&D Struct Res Lab Unnastr 48 D-20245 Hamburg Germany Univ Hamburg Dept Informat Cognit Syst Lab D-22527 Hamburg Germany

ISBN: (纸本)9780819479662

Examining a volunteer or patient in-vivo usually is a strictly time-constrained process. When complex (custom) multicomponent, multi-modal imaging devices are involved, an examination session might be significantly stressful for the experimenter who is in charge of instrumental control. This is especially true when many parameters, several different devices or several different software applications are to be controlled during a single session. Stress may increase the probability of suboptimal data acquisition and data loss. The experimenter should be focused on the sample, not on instrumental control. In order to ease image acquisition, we present concepts for a new intuitive and interactive software application that integrates control of all hardware components, guides the experimenter through customizable acquisition workflows, presents the recorded data ready for facilitated interpretation using state-of-the-art image processing algorithms and thus supports the experimenter in acquiring optimized and highly valuable image data. Our concept combines into a single application many functions that were previously performed by several different programs, it allows linking together the functionalities, it allows automation, and it adds several convenience and safety features.

关键词： microscopy image acquisition image analysis software automatization

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Dual-mode digital holographic and fluorescence microscopy for the study of morphological changes in cells under simulated microgravity

Dual-mode digital holographic and fluorescence microscopy fo...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Toy, M. Fatih Pache, Christophe Parent, Jerome Kuehn, Jonas Egli, Marcel Depeursinge, Christian Ecole Polytech Fed Lausanne Adv Photon Lab CH-1015 Lausanne Switzerland ETH Space Biol Grp CH-8005 Zurich Switzerland

ISBN: (纸本)9780819479662

A dual mode microscope is developed to study morphological evolution of mouse myoblast cells under simulated microgravity in real time. Microscope operates in Digital Holographic microscopy (DHM) and widefield epifluorescence microscopy modes in a time sequential basis. DHM provides information on real time cellular morphology. EGFP transfected actin filaments in mouse myoblast cells function as the reporter for the fluorescence microscopy mode. Experimental setup is fixed in the RPM to observe microgravity induced dynamic changes in live cells. Initial results revealed two different modifications. Disorganized structures become visible in the formed lamellipodias, and proteins accumulate in the perinuclear region.

关键词： microscopy holography digital holographic microscopy fluorescence microscopy microgravity cell morphology cytoskeleton

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LABEL-FREE CLASSIFICATION OF CELL TYPES BY IMAGING OF CELL MEMBRANE FLUCTUATIONS USING LOW-COHERENT FULL-FIELD QUANTITATIVE PHASE microscopy

LABEL-FREE CLASSIFICATION OF CELL TYPES BY IMAGING OF CELL M...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Yamauchi, Toyohiko Sugiyama, Norikazu Sakurai, Takashi Iwai, Hidenao Yamashita, Yutaka Hamamatsu Photon KK Hamakita Ku 5000 Hirakuchi Hamamatsu Shizuoka 4348601 Japan MIT Spectroscopy Lab Cambridge MA 02139 USA Hamamatsu Univ Sch Med Photon Med Res Ctr Hamamatsu Shizuoka 4313192 Japan

ISBN: (纸本)9780819479662

Cell membrane motions of living cells are quantitatively measured in nanometer resolution by low-coherent full-field quantitative phase microscopy. Our setup is based on a full-field phase shifting interference microscope with a very low-coherent light source. The reflection mode configuration and the low-coherent illumination make it possible to differentiate the weak reflection light from the cell membrane from the strong reflection from the glass substrate. Two cell populations are quantitatively assessed by the power spectral density of the cell surface motion and show different trends.

关键词： quantitative phase microscope cell imaging plasma membrane

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