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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXII"

共 590 条记录，以下是411-420 订阅

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three-dimensional state space realization algorithm: noise suppression of fluorescence microscopy images and point spread functions

Three-dimensional state space realization algorithm: noise s...

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Lai, XM Ward, ES Lin, ZP Ober, RJ Univ Texas SW Med Ctr Ctr Immunol Dallas TX 75235 USA

ISBN: (纸本)0819456756

A recently developed algorithm is applied to calculate a state space realization of a 3D microscopy image set. It is based on interpreting the image set as the impulse response of a 3D se arable system. As an application it is shown how this algorithm. combined with approximation steps, can be used to suppress noise in 3D experimental point spread functions. The approach was motivated by a well known problem that a noisy point spread function degrades the results of deconvolution algorithms for the restoration of 3D fluorescence microscopy image sets. The proposed approach can also be applied to 3D fluorescence microscopy image sets of cells.

关键词： point spread function estimation state space algorithm noise suppression

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High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera

High speed slit-scanning confocal laser microscopy with an a...

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Han, SM Im, KB Park, H Kim, D Kim, BM Yonsei Univ Dept Biomed Engn Kangwon Do 220710 South Korea

ISBN: (纸本)0819456756

Using an acousto-optic beam deflector and a line scan camera, we constructed a non-mechanical, slit-scanning confocal microscope. It generates two-dimensional 512x512 images with speed of 60 frames/sec, which can be easily expanded, to 100 frames/sec. The measured axial and lateral resolutions of the system are 3.3 mu m and < 1 mu m, respectively, with a 50X objective. This simple design can produce frames rates faster than other commercial products with comparable resolution, which may be useful for analysis of rapid interactions in various biological applications.

关键词： confocal microscope three-dimensional microscopy acousto-optic deflector line scan CCD

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Tomographic imagery by interference microscopy

Tomographic imagery by interference microscopy

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Dubois, A Guiot, E Schwartz, W Moneron, G Grieve, K Boccara, C CNRS UPR A0005 Ecole Super Phys & Chim Ind Lab Opt Phys F-75231 Paris France

ISBN: (纸本)0819456756

We have developed a white-light interference microscope as an alternative technique to conventional optical coherence tomography (OCT). The experimental setup is based on a Linnik interferometer illuminated with a tungsten halogen lamp. En face tomographic images are obtained in real-time without scanning by computing the difference of two phase-opposed interferometric images recorded by a CCD camera. The short coherence length of the source yields an optical sectioning ability with 0.7 mu m resolution (in water). Transverse resolution of 0.9 mu m is achieved by using high numerical aperture microscope objectives. A shot-noise limited detection sensitivity of 90 dB can be reached with similar to 1 s acquisition time. High-resolution images of mouse and tadpole embryos are shown.

关键词： interference microscopy three-dimensional microscopy optical coherence tomography medical and biomedical imaging

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Using out-of-focus light to improve image acquisition time in confocal microscopy

Using out-of-focus light to improve image acquisition time i...

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Davis, BJ Karl, WC Goldberg, BB Swan, AK Ünlü, MS Boston Univ Dept Elect & Comp Engn Boston MA 02215 USA

ISBN: (纸本)0819456756

Light that would typically be discarded at a confocal microscope's detector pinhole will be collected and processed to allow a reduced spatial sampling rate and thus an improved image acquisition time. It is shown that collecting and appropriately processing the out-of-focus light will allow an axial sampling rate below that specified by the Nyquist criterion. To achieve this, a central detector pinhole and a number of out-of-focus regions are collected concurrently. This corresponds to imaging through several different channels, with differing point spread functions, in parallel. Since the spatial sampling rate is below the Nyquist frequency, aliasing occurs in the data from each of the channels. However, since the point spread functions are different, the aliasing effects are different in each channel. This allows the ensemble of aliased images to be processed into a sin-le dealiased and deconvolved image. This potential utility of out-of-focus light is demonstrated through simulated examples for differing collection schemes and scanning rates. Results are shown for under-sampling by up to a factor of four. Collecting the out-of-focus light also improves instrument collection efficiency.

关键词： confocal microscopy image acquisition time sampling rate aliasing out-of-focus light confocal pinhole high speed microscopy confocal collection efficiency

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A wide-field microscopy technique using a linear image sensor for obtaining 3D images

A wide-field microscopy technique using a linear image senso...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Macedo, MP Barata, AJ Fernandes, AG Correia, CM Inst Engn Coimbra Dept Phys & Math P-3030 Coimbra Portugal

ISBN: (纸本)0819456756

three-dimensional (3D) microscopy is a huge field that includes several microscopy techniques. Among these techniques the confocal microscopy is widely known and used due to its improved axial resolution or optical sectioning ability. However its high cost and image acquisition time as well as low signal-to-noise ratio are important drawbacks. Other techniques use wide-field structured illumination to get depth information from the sample. The detectors in commercial microscopes are normally of the point detector type (PMTs) in confocal and area sensors (CCDs) in widefield microscopy. Examples of microscopes that take advantage of lower cost and faster readout cycles of linear image sensors are very uncommon. The purpose of this work is to develop a low-cost microscopy technique for obtaining 3D images with lower acquisition time and higher signal-to-noise ratio (SNR) than in classical confocal microcopy. It uses a linear CMOS image sensor and specific reconstruction algorithms will be able to extract 3D information from the distribution of light intensity collected. The sensor readout circuitry has been developed and tests are currently running in a laboratory prototype in reflection mode with an epi-illuminated configuration of scanning-stage type. The light source is a commercial incandescent lamp with regulated intensity. For the development, test and optimization of the reconstruction algorithms the object is mounted on a 3-axis translation stage to scan the object in three perpendicular directions. Contrast and resolution results obtained using a resolution test slide and preliminary images of integrated circuit (IC) bonding are presented.

关键词： 3D microscopy confocal microscopy reconstruction algorithms optical sectioning linear sensor

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Multimodal optical microscope for imaging biological samples

Multimodal optical microscope for imaging biological samples

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Townsend, DJ DiMarzio, CA Laevsky, G Rajadhvaksha, M Northeastern Univ Ctr Subsurface Sensing & Imaging Syst Boston MA 02115 USA

ISBN: (纸本)0819456756

A multimodal microscope provides a researcher with the ability to capture images with multiple sources of contrast. Previous attempts at multimodal microscopy have been limited to capturing images at different instruments and digitally registering the images empirically using features common in the specimen. Other multimodal microscopes have combined different microscopy techniques, but have been limited in their sources of contrast. We present a unique microscope which contains 5 different imaging modalities, with four different sources of contrast. Quadrature microscopy measures the phase of the electric field of the light transmitted through an optically transparent specimen, by interference with a reference beam, using a polarimetric technique to resolve the ambiguities between phase and amplitude. Differential Interference Contrast (DIC) measures the phase gradient in an optically transparent specimen by combining two spatially separated images interferometrically. Confocal reflectance measures index of refraction changes in a plane or reflection from small scatterers. Confocal fluorescence and 2-photon laser scanning microscopy, measure fluorescent signatures of a specimen. The last three of these are inherently capable of producing three-dimensional images directly, through localized probing.

关键词： multimodal microscopy quadrature confocal

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Visualization of nanocontractions in cardiomyocytes by simultaneous detection of second- and third-harmonic generation and multiphoton excitation fluorescence microscopy

Visualization of nanocontractions in cardiomyocytes by simul...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Greenhalgh, C Barzda, V Elmore, S van Beeo, JHGM Der Au, JA Squier, J Univ Toronto Dept Phys Mississauga ON L5L 1C6 Canada

ISBN: (纸本)0819456756

Dynamic imaging of cardiomyocytes was performed with a simultaneous detection second harmonic generation (SHG), third harmonic generation (THG) and multiphoton excitation fluorescence (MPF) microscope. The fast scanning system of similar to 12 frames/second synchronized with multichannel detection provided the possibility of imaging three dimensional static and two dimensional dynamic structures of cardiomyocytes. The SHG images highlighted the myofibrils of the cardiomyocytes while THG images revealed the locations of mitochondria. Dynamic data showed that during imaging, chaotic nanocontractions took place inside the cardiomyocytes. The time series of THG images reveled large intensity fluctuations i flickeringi in the regions of mitochondria. The flickering in THG correlated with the flickering in MPF. Addition of the uncoupler FCCP inhibited flickering in THG and MPF, and also inhibited nanocontractions. The simultaneous imaging with SHG, THG and MPF proved to be a very powerful microscopy tool for investigation of interactions of different organelles inside a cell.

关键词： harmonic generation imaging femtosecond laser scanning microscopy dynamic microscopy biological imaging contractility of myocytes mitochondria bioenergetics

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Microlymphatic flow using fast video microscopy

Microlymphatic flow using fast video microscopy

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Dixon, JB Zawieja, DC Gashev, AA Coté, GL Texas A&M Univ Dept Biomed Engn College Stn TX 77843 USA

ISBN: (纸本)0819456756

Despite advances in the measurement of lymphatic flow, little is known about the actual velocities of flow in microlymphatic (similar to 100 um diameter) vessels. In this paper, video microscopy and particle tracking methods were adapted and integrated with an ultra high-speed imaging camera to obtain measurements of high-speed lymph velocities that previous systems were incapable of measuring. In this study, a mesenteric microlymphatic vessel in a loop of the small intestine of a male Sprague-Dawley rat was exteriorized and imaged at a rate of 500 frames per second (fps) for several contraction sequences. Lymph velocity was shown to fluctuate cyclically with the vessel wall contractions and ranged from -1 to 4 mm/sec through a ten second sequence.

关键词： Lymphatic system Cameras Velocity measurements Video microscopy Microfluidics Imaging systems High speed cameras Microfluidic imaging Video Particles

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three-dimensional optical computed-tomography microscope

Three-dimensional optical computed-tomography microscope

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Chamgoulov, R Lane, P MacAulay, C British Columbia Canc Res Ctr Canc Imaging Dept Vancouver BC V5Z 1L3 Canada

ISBN: (纸本)0819456756

We present our recent results on the development of three-dimensional (3-D) optical computed-tomography microscope. The instrument is a novel imaging device for the 3-D visualization and quantitative analysis of absorption-stained biological samples. The first instrument developed by our group at the BC Cancer Research Centre used a digital micromirror device (DMD) as a spatial light modulator to control the angles of illumination. This new embodiment employs an optical scanner instead of the DMD. The optical scanner is placed in the illumination path of the microscope system, conjugate to the field plane. The optical system includes also two high numerical aperture objective lenses, a sample stage, a light source, and a CCD camera. Projections are acquired by illuminating a specimen at a number of selected angles within the numerical aperture of the objective (0 <= phi <= 135 degrees). A new reconstruction algorithm that employs both transform-based and iterative methods is developed to address the limited-angle reconstruction problem. A transform-based reconstruction is used as an initial starting point for the following iterative reconstruction. A feedback correction of the reconstructed image is made on each iteration step. The algorithm enables to incorporate previously known information about the object into the reconstruction process, and improves the reconstruction accuracy. Microscopic 3-D volume reconstructions of quantitatively absorption-stained cells have been generated. The system enables one to look at multiple optical levels of a specimen, and at more natural tissue architecture, including intact cells. Axial and lateral resolutions were measured to be better than 6 microns.

关键词： optical microscopy 3-D imaging computed tomography limited-angle reconstruction

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Variable-focal length microlens arrays in confocal microscopy.

Variable-focal length microlens arrays in confocal microscop...

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Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Mac Raighne, A Wang, JG Cabe, EM Scharf, T Trinity Coll Dublin Dept Phys Dublin Ireland

ISBN: (纸本)0819456756

Microlenses have been implemented in confocal systems successfully as components of aperture arrays and as arrays of objective lenses. The use of the novel technology of variable focal length (VFL) microlenses in the confocal system is investigated. The use of VFL microlenses as an aperture array in conjunction with an optical fiber as a pinhole array is examined. Axial responses of the system where measured and the Full-Width Half Maximum (FWHM) found to be similar to 16 mu m. VFL microlenses as an array of objective lenses is investigated with a novel method for scanning in the axial direction presented. By variation of the focal length of the lenses the focal plane can be scanned through the sample without the mechanical movement of the sample or the objective lens, we have named this 'focal scanning'. It is shown that the limiting factor with this type of scanning is the low numerical aperture (NA) of the microlenses available. Both focal scanning and conventional scanning are examined for this experimental set-up.

关键词： confocal microscopy variable focal length microlenses axial scanning

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