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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX 2022"

共 602 条记录，以下是11-20 订阅

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three-dimensional multi-directional light-sheet microscopy with structured illumination 31

Three-dimensional multi-directional light-sheet microscopy w...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXXI 2024

作者： Song, Ming Liu, Yang Wu, Hsueh-Fu Zeltner, Nadja Kner, Peter A. School of Electrical and Computer Engineering University of Georgia AthensGA United States Chan Zuckerberg Biohub San FranciscoCA United States Center for Molecular Medicine University of Georgia AthensGA United States Department of Biochemistry and Molecular Biology University of Georgia AthensGA United States Department of Cellular Biology University of Georgia AthensGA United States

ISBN: (纸本)9781510669550

Light-Sheet Fluorescence microscopy (LSFM) has demonstrated its effectiveness in imaging many model organisms. However, traditional LSFMs often exhibit low resolution and encounter striping artifacts. Here, we introduce a novel LSFM design to address these challenges of imaging large biological samples. Our system employs a single-objective light-sheet geometry, incorporating Structured Illumination microscopy (SIM) to allow multi-direction light-sheet illumination. This combination aims to rectify striping artifacts and achieve improved lateral resolutions of better than 200 nm over a 277 × 277 × 500 μm3 volume. Our system is demonstrated through imaging beads, C. elegans and cerebellum organoids. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

关键词： Fluorescence microscopy

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Imaging the brain in vivo with reflectance phase-contrast confocal microscopy at 1650nm 31

Imaging the brain in vivo with reflectance phase-contrast co...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXXI 2024

作者： Delafontaine Martel, P. Zhang, Cong Linninger, Andreas A. Lesage, Frédéric Biomedical Engineering Institute Polytechnique Montreal Montreal Canada Montreal Heart Institute Montreal Canada Departement of Biomedical Engineering and Neurosurgery University of Illinois ChicagoIL United States

ISBN: (纸本)9781510669550

Recent advancements in sources and detectors operating in the NIR-II wavelengths have driven the emergence of NIR-II intrinsic microscopy. These significant technological strides were necessary because longer wavelengths are known to experience reduced scattering and absorption in biological tissue. Leveraging this optical advantage, the application of the NIR-II spectral domain in microscopy holds the potential to improve the depth of imaging and preserve coherence depth. In this study, we showcase the integration of phase-contrast imaging into a NIR-II reflectance confocal microscope for cortical imaging. By capturing images of cortical cell bodies at depths of up to 800 μm, we demonstrate that the implementation of phase contrast provides clear delineation of cortical cell edges, including myelinated axons, blood vessels, and cortical cell bodies. Additionally, we devised a computational method to enhance dynamic components and generate a digitized vascular network from the acquired images. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

关键词： Reflection

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Optical sectioning microscopy with structured illumination and single-pixel detection 30

Optical sectioning microscopy with structured illumination a...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXX 2023

作者： Ordóñez, Luis Ipus, Erick Lenz, Armin J.M. Lancis, Jesús Tajahuerce, Enrique Universitat Jaume I Castelló12071 Spain

ISBN: (纸本)9781510658752

We propose a new single pixel microscope with optical sectioning properties by using structured illumination techniques. The method uses single-pixel imaging (SPI) techniques by interrogating the sample with a series of spatially resolved patterns and measuring the output intensities with a non-spatial resolution detector. Moreover, optical sectioning is obtained by adding a grating to the system and employing structured illumination microscopy (SIM). The system allows us to perform 3D bright-field and fluorescence microscopy. We apply compressive sensing techniques to decrease the acquisition time. © 2023 SPIE. All rights reserved.

关键词： Fluorescence microscopy

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Automated chondrocyte viability analysis of articular cartilage based on deep learning segmentation and classification of two-photon microscopic images 29

Automated chondrocyte viability analysis of articular cartil...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xxix

作者： Fan, Hongming Xu, Pei Le, Michael Hsu, Jennifer Chen, Xun Li, Yang Zhang, Zhao Gao, Bruce Woolf, Shane Ye, Tong Clemson Univ Dept Bioengn Clemson SC 29634 USA Clemson Univ Sch Comp Clemson SC USA Med Univ South Carolina Dept Orthopaed & Phys Med Charleston SC 29425 USA Med Univ South Carolina Dept Regenerat Med & Cell Biol Charleston SC 29425 USA

ISBN: (纸本)9781510648043;9781510648036

Chondrocyte viability is an important measure to consider when assessing cartilage health. Dye-based cell viability assays are not suitable for in vivo or long-term studies. We have introduced a non-labeling viability assay based on the assessment of high-resolution images of cells and collagen structure using two-photon stimulated autofluorescence and second harmonic generation microscopy. By either the visual or quantitative assessment, we were able to differentiate living from dead chondrocytes in those images. However, both techniques require human participation and have limited throughputs. Throughput can be increased by using methods for automated cell-based image processing. Due to the poor image contrast, traditional image processing methods are ineffective on autofluorescence images produced by nonlinear microscopes. In this work, we examined chondrocyte segmentation and classification using Mask R-CNN, a deep learning approach to implement automated viability analysis. It has been demonstrated an 85% accuracy in chondrocyte viability assessment with proper training. This study demonstrates that automated and highly accurate image analysis is achievable with the use of deep learning methods. This image processing approach can be helpful to other imaging applications in clinical medicine and biological research.

关键词： nonlinear optical microscopy autofluorescence second harmonic generation deep learning viability

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image projection through a multimode fiber with reference-lessly retrieved transmission matrix 31

Image projection through a multimode fiber with reference-le...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXXI 2024

作者： Zhong, Jingshan Wen, Zhong Li, Quanzhi Deng, Qilin Yang, Qing Research Center for Humanoid Sensing Zhejiang Lab Hangzhou311100 China State Key Laboratory of Extreme Photonics and Instrumentation College of Optical Science and Engineering International Research Center for Advanced Photonics Zhejiang University Hangzhou310027 China

ISBN: (纸本)9781510669550

image projection through a multimode fiber (MMF) or scattering media has applications ranging from optogenetics to near eye-displays. It requires developing computer-generated holography algorithm to obtain phase pattern of spatial light modulator. In order to accurately project light, conventional methods measured the transmission matrix (TM) of the imaging system by interference. However, it is sensitive to phase instability, easily caused by thermal drift and mechanical vibration. In this work, we proposed to use the TM retrieved from intensity-only measurements and develop a nonlinear optimization algorithm to obtain the displayed phase patterns. Our method formulates the forward model with the retrieved TM, derives the analytical derivative and adopts a second-order optimization method. We validate the improved quality of the projected intensity image by an experiment setup with a MMF. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

关键词： Multimode fibers

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Multispectral digital holographic microscopy with tunable supercontinuum lasers 31

Multispectral digital holographic microscopy with tunable su...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXXI 2024

作者： Kurtz, Tobias Barroso, Álvaro Picazo-Bueno, José Ángel Ketelhut, Steffi Schnekenburger, Jürgen Kemper, Björn Biomedical Technology Center of the Medical Faculty University of Muenster MuensterD-48149 Germany Department Department of Optics Optometry and Vision Science Faculty of Physics University of Valencia Burjassot46100 Spain

ISBN: (纸本)9781510669550

Supercontinuum (SC) lasers in combination with tunable acousto-optic filters facilitate multiwavelength digital holographic microscopy (DHM) with a broad spectral range. However, to achieve optimized quantitative phase imaging (QPI) in off-axis DHM the coherence properties of the tunable light source and the holographic carrier interference fringe pattern formation have to be considered as they are wavelength dependent. We thus determined experimentally the wavelength dependency of coherence length and off-axis carrier fringe frequency in a Michelson interferometer-based multi-spectral DHM setup with a continuously tunable SC laser and moreover analyzed the impact of both parameters on hologram formation and QPI image quality. The results are found in good agreement with theoretical predictions and provide essential information for a reliable adjustment of SC laser based multispectral off-axis DHM systems. Finally, the application of multispectral-DHM for reduction of coherence induced disturbances in quantitative phase images of living pancreatic tumor cells in illustrated. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

关键词： Michelson interferometers

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Low dosage 3D volume fluorescence microscopy imaging using compressive sensing 29

Low dosage 3D volume fluorescence microscopy imaging using c...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing xxix

作者： Mannam, Varun Brandt, Jacob Smith, Cody J. Howard, Scott Univ Notre Dame Dept Elect Engn Notre Dame IN 46556 USA Univ Notre Dame Dept Biol Sci Notre Dame IN 46556 USA

ISBN: (纸本)9781510648043;9781510648036

Fluorescence microscopy has been a significant tool to observe long-term imaging of embryos (in vivo) growth over time. However, cumulative exposure is phototoxic to such sensitive live samples. While techniques like light-sheet fluorescence microscopy (LSFM) allows for reduced exposure, it is not well suited for deep imaging models. Other computational techniques are computationally expensive and often lack restoration quality. To address this challenge, one can use various low-dosage imaging techniques that are developed to achieve the 3D volume reconstruction using a few slices in the axial direction (z-axis);however, they often lack restoration quality. Also, acquiring dense images (with small steps) in the axial direction is computationally expensive. To address this challenge, we present a compressive sensing (CS) based approach to fully reconstruct 3D volumes with the same signal-to-noise ratio (SNR) with less than half of the excitation dosage. We present the theory and experimentally validate the approach. To demonstrate our technique, we capture a 3D volume of the RFP labeled neurons in the zebrafish embryo spinal cord (30 mu m thickness) with the axial sampling of 0.1 mu m using a confocal microscope. From the results, we observe the CS-based approach achieves accurate 3D volume reconstruction from less than 20% of the entire stack optical sections. The developed CS-based methodology in this work can be easily applied to other deep imaging modalities such as two-photon and light-sheet microscopy, where reducing sample photo-toxicity is a critical challenge.

关键词： Low-dosage imaging 3D volume reconstruction fluorescence microscopy compressive sensing (CS) discrete cosine transform (DCT) deep imaging in vivo imaging

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Super-resolution in confocal microscopy using generative adversarial networks with paired and unpaired data 30

Super-resolution in confocal microscopy using generative adv...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXX 2023

作者： Patra, S. Trujillo, C. Doblas, A. Department of Electrical and Computer Engineering The University of Memphis TN United States School of Applied Sciences and Engineering Universidad EAFIT Medellin Colombia

ISBN: (纸本)9781510658752

The advantages of confocal microscopy over widefield microscopy are their ability to produce optically sectioned images and their ability to produce multi-color imaging in which different organelles within the biological specimen are stained using multiple dyes, enabling colocalization studies. These features make confocal microscopy a widely used tool to provide valuable morphological and functional information within cells and tissues. One of the major drawbacks of confocal microscopy is its limited spatial resolution. Here, we train two generative adversarial networks using paired and unpaired data of low- and high-resolution images to improve the spatial resolution in confocal microscopy. © 2023 SPIE. All rights reserved.

关键词： Confocal microscopy

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Utilizing fluorescent beads to mimic single fluorophores 30

Utilizing fluorescent beads to mimic single fluorophores

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three-dimensional and multidimensional microscopy: image acquisition and processing XXX 2023

作者： Feng, Sherry Yi-Ting Alemán-Castañeda, Luis A. Gutiérrez-Cuevas, Rodrigo Herrera, Isael Brown, Thomas G. Brasselet, Sophie Alonso, Miguel A. The Institute of Optics University of Rochester RochesterNY14627 United States Aix Marseille Univ CNRS Centrale Marseille Institut Fresnel MarseilleF-13013 France Institut Langevin ESPCI Paris Université PSL CNRS Paris75005 France

In this paper, numerical and semi-analytical methods are used for studying how well a fluorescent bead with a given radius in a polarizer-supplemented microscopy setup, can emulate a single fluorescent molecule. ©... 详细信息

ISBN: (纸本)9781510658752

关键词： Fluorescence microscopy

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Hyperspectral microscopy as a particle exposure assessment tool 31

Hyperspectral microscopy as a particle exposure assessment t...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXXI 2024

作者： Blanch-Perez Del Notario, Carolina Bertier, Pieter Jayapala, Murali Lambrechts, Andy Imec Kapeldreef 75 Leuven3001 Belgium Belgian Center for Occupational Hygiene BeCOH Brusselsesteenweg 46 Leuven3000 Belgium

ISBN: (纸本)9781510669550

Hyperspectral imaging combines the characteristics of computer vision and point spectroscopy by obtaining an image with both spatial and spectral information. Therefore, in combination with microscopy, it can increase material discrimination possibilities with respect to regular microscopy imaging. We explore this increased discrimination potential to assess exposure to particle contamination, since workplace exposure to specific particle materials poses well-known health hazards. In this respect, we are focusing on discriminating more health relevant particles such as silica in the respirable size fraction. For this purpose, a particle sampling protocol has been proposed and hyperspectral imaging in combination with transmission microscopy is used for particle material identification. We use a Snapscan visual near-infrared (VNIR) camera providing high spectral and spatial resolution in the 460-900 nm range, 150 spectral bands and up to 7 Mpixels of spatial resolution and high acquisition speed. The hyperspectral microscopy system has been tested for discrimination of fifteen different particle materials, such as silica, coal, dolomite, barite, or rutile, among others. The combined analysis of spatial and spectral information shows potential to accurately discriminate the 15 tested particle materials so far by means of a random forest classifier. In addition, a band relevance analysis is performed showing that only a few specific bands are needed to provide accurate discrimination of the tested materials. The hyperspectral hardware and method presented could lead to a faster exposure assessment than traditional techniques used for occupational exposure estimation. © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

关键词： Health hazards

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