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检索条件"任意字段=Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX 2022"

共 602 条记录，以下是191-200 订阅

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Investigation of the SQUBIC phase mask design for depth-invariant widefield microscopy point-spread function engineering

Investigation of the SQUBIC phase mask design for depth-inva...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Doblas, Ana King, Sharon V. Patwary, Nurmohammed Saavedra, Genaro Martinez-Corral, Manuel Preza, Chrysanthe Univ Valencia Dept Opt E-46100 Burjassot Spain Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA

ISBN: (纸本)9780819498625

Point-spread function engineering (PSF), achieved by placing a phase mask at the pupil plane of the imaging lens to encode the wavefront emerging from an imaging system, can be employed to reduce the impact of spherical aberration (SA) in 3D microscopy. In a previous study, the effect of SA on a confocal scanning microscope using a squared cubic phase mask (SQUBIC) was investigated using computer simulations. Here the effect of the SQUBIC design parameter on the insensitivity of the engineered PSF to SA is investigated using a metric based on the second-order moment of the axial variability of the PSF. We show that it is possible to find the optimum SQUBIC for the insensitization to SA.

关键词： Point-spread function engineering wavefront coding spherical aberration

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Reducing depth induced spherical aberration in 3D widefield fluorescence microscopy by wavefront coding using the SQUBIC phase mask

Reducing depth induced spherical aberration in 3D widefield ...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Patwary, Nurmohammed Doblas, Ana King, Sharon V. Preza, Chrysanthe Univ Memphis Dept Elect & Comp Engn Memphis TN 38152 USA Univ Valencia Dept Opt E-46100 Burjassot Spain

ISBN: (纸本)9780819498625

Imaging thick biological samples introduces spherical aberration (SA) due to refractive index (RI) mismatch between specimen and imaging lens immersion medium. SA increases with the increase of either depth or RI mismatch. Therefore, it is difficult to find a static compensator for SA(1). Different wavefront coding methods(2,3) have been studied to find an optimal way of static wavefront correction to reduce depth-induced SA. Inspired by a recent design of a radially symmetric squared cubic (SQUBIC) phase mask that was tested for scanning confocal microscopy(1) we have modified the pupil using the SQUBIC mask to engineer the point spread function (PSF) of a wide field fluorescence microscope. In this study, simulated images of a thick test object were generated using a wavefront encoded engineered PSF (WFE-PSF) and were restored using space-invariant (SI) and depth-variant (DV) expectation maximization (EM) algorithms implemented in the COSMOS software(4). Quantitative comparisons between restorations obtained with both the conventional and WFE PSFs are presented. Simulations show that, in the presence of SA, the use of the SIEM algorithm and a single SQUBIC encoded WFE-PSF can yield adequate image restoration. In addition, in the presence of a large amount of SA, it is possible to get adequate results using the DVEM with fewer DV-PSFs than would typically be required for processing images acquired with a clear circular aperture (CCA) PSF. This result implies that modification of a widefield system with the SQUBIC mask renders the system less sensitive to depth-induced SA and suitable for imaging samples at larger optical depths.

关键词： three-dimensional microscopy wavefront encoding point-spread function engineering image restoration phase mask

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Customized Profile Lens Based Linear Response Fast Spinning On-axis Optical Scanners for 3D Laser Scanning microscopy

Customized Profile Lens Based Linear Response Fast Spinning ...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Lai, Zhenhua Mega, Yair J. Yang, Xibin Zhu, Jianfeng Gu, Zetong Xiong, Daxi DiMarzio, Charles A. Northeastern Univ Dept Elect & Comp Engn Boston MA 02115 USA Northeastern Univ Dept Mech & Ind Engn Boston MA 02115 USA Chinese Acad Sci Suzhou Inst Biomed Engn & Technol Jiangsu Key Lab Med Optic Suzhou Peoples R China

ISBN: (纸本)9780819498625

Previously, we presented an on-axis linear-response linear-motion optical scanner. While the linear design is highly desired for engineering consideration, it was still lacking the scanning speed required for imaging applications. We here present a customized profile lens (CPL), tailored for high speed performance while maintaining the advantages of a linear response on-axis optical scanner. The device was built and tested experimentally on an optical bench. The test results demonstrate precise linear response and fast scanning speed, and revealed video frame rate scanning ability. The implementation of the CPLs in laser scanning systems is promising in improving the current 3D laser scanning microscopy systems by reducing the size, error, and complexity of the system, as well as other systems unitizing high speed laser scanning technique.

关键词： Beam Steering Laser Scanning microscopy Optical Scanner

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Real-time stroboscopic full-field optical coherence tomography based on graphics processing unit

Real-time stroboscopic full-field optical coherence tomograp...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Park, Kwan Seob Kim, Hyounggyu Kim, Ju Wan Baek, Hee Gyu Lee, Jae Hwi Chung, Youngjoo Lee, Byeong Ha Gwangju Inst Sci & Technol Sch Informat & Commun 261 Cheomdan Gwagiro Kwangju 500712 South Korea Gwangju Inst Sci & Technol Dept Med Syst Engn Gwangju 500712 South Korea

ISBN: (纸本)9780819498625

We present the real-time stroboscopic full-field optical coherence tomography (FF-OCT) system that is based on graphics processing unit (GPU). The basic configuration of the proposed FF-OCT system was the Linnik interferometer. While scanning of a reference mirror in the axial direction, a series of the transverse sectional image was captured with a 2-dimensional CCD camera. To get a depth-resolved 3-D image, the light source of OCT was turned on and off like a stroboscope at the Doppler frequency of the OCT system. The CCD camera used in experiment operated at a rate of 200 frames per second, but the Doppler frequency was similar to kHz. To overcome the slow operation of the CCD camera below the Doppler frequency, the light source was operated in the stroboscopic mode. In addition, lock-in detection technique was utilized in order to avoid the dissolution of the coherent signals during the integration time of the CCD camera. Furthermore, the Doppler frequency shift due to nonlinear scanning motion of the reference mirror was monitored by using an auxiliary interferometer and then fed back to the light source driver so that the strobe frequency was always matched with the Doppler frequency of the OCT system. For the real-time 3-D rendering, we used a graphics processing unit.

关键词： full-field optical coherence tomography stroboscopic illumination graphics processing unit real-time rendering

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Wavefront shaping of a Bessel light field enhances light sheet microscopy with scattered light

Wavefront shaping of a Bessel light field enhances light she...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Nylk, J. Mitchell, C. Vettenburg, T. Gunn-Moore, F. J. Dholakia, K. Univ St Andrews Sch Phys & Astron SUPA St Andrews KY16 9SS Fife Scotland Univ St Andrews Sch Biol St Andrews KY16 9TF Fife Scotland

ISBN: (纸本)9780819498625

Light sheet microscopy has seen a resurgence as it facilitates rapid, high contrast, volumetric imaging with minimal sample exposure. Initially developed for imaging scattered light, this application of light sheet microscopy has largely been overlooked but provides an endogenous contrast mechanism which can complement fluorescence imaging and requires very little or no modification to an existing light sheet fluorescence microscope. Fluorescence imaging and scattered light imaging differ in terms of image formation. In the former the detected light is incoherent and weak whereas in the latter the coherence properties of the illumination source, typically a laser, dictate the coherence of detected light, but both are dependent on the quality of the illuminating light sheet. image formation in both schemes can be understood as the convolution of the light sheet with the specimen distribution. In this paper we explore wavefront shaping for the enhancement of light sheet microscopy with scattered light. We show experimental verification of this result, demonstrating the use of the propagation invariant Bessel beam to extend the field of view of a high resolution scattered light, light sheet microscope and its application to imaging of biological super-cellular structures with sub-cellular resolution. Additionally, complementary scattering and fluorescence imaging is used to characterize the enhancement, and to develop a deeper understanding of the differences of image formation between contrast mechanisms in light sheet microscopy.

关键词： Light sheet microscopy LSM selective plane illumination microscopy SPIM beam shaping Bessel beam high resolution deconvolution

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in vivo Stepwise Multi-Photon Activation Fluorescence Imaging of Melanin in Human Skin

<i>in vivo</i> Stepwise Multi-Photon Activation Fluorescence...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Lai, Zhenhua Gu, Zetong Abbas, Saleh Lowe, Jared Sierra, Heidy Rajadhyaksha, Milind DiMarzio, Charles Northeastern Univ Dept Elect & Comp Engn Boston MA 02115 USA Northeastern Univ Biol Dept Boston MA 02115 USA Northeastern Univ Mech & Ind Engn Dept Boston MA 02115 USA Mem & Sloan Kettering Canc Ctr Dermatol Serv New York NY 10065 USA Bernard M Gordon Ctr Subsurface Sensing & Imagin San Juan PR 00931 USA

ISBN: (纸本)9780819498625

The stepwise multi-photon activated fluorescence (SMPAF) of melanin is a low cost and reliable method of detecting melanin because the activation and excitation can be a continuous-wave (CW) mode near infrared (NIR) laser. Our previous work has demonstrated the melanin SMPAF images in sepia melanin, mouse hair, and mouse skin. In this study, we show the feasibility of using SMPAF to detect melanin in vivo. in vivo melanin SMPAF images of normal skin and benign nevus are demonstrated. SMPAF images add specificity for melanin detection than MPFM images and CRM images. Melanin SMPAF is a promising technology to enable early detection of melanoma for dermatologists.

关键词： fluorescence stepwise multi-photon excitation melanin microscopy near-infrared

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Quantitative Phase microscopy: how to make phase data meaningful

Quantitative Phase Microscopy: how to make phase data meanin...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Goldstein, Goldie Creath, Katherine 4D Technol Corp Tucson AZ 85706 USA

ISBN: (纸本)9780819498625

The continued development of hardware and associated image processing techniques for quantitative phase microscopy has allowed superior phase data to be acquired that readily shows dynamic optical volume changes and enables particle tracking. Recent efforts have focused on tying phase data and associated metrics to cell morphology. One challenge in measuring biological objects using interferometrically obtained phase information is achieving consistent phase unwrapping and background shape removal throughout a sequence of images. Work has been done to improve the phase unwrapping in two- dimensions and correct for temporal discrepanices using a temporal unwrapping procedure. The residual background shape due to mean value fluctuations and residual tilts can be removed automatically using a simple object characterization algorithm. Once the phase data are processed consistently, it is then possible to characterize biological samples such as myocytes and myoblasts in terms of their size, texture and optical volume and track those features dynamically. By observing optical volume dynamically it is possible to determine the presence of objects such as vesicles within myoblasts even when they are co-located with other objects. Quantitative phase microscopy provides a label-free mechanism to characterize living cells and their morphology in dynamic environments, however it is critical to connect the measured phase to important biological function for this measurement modality to prove useful to a broader scientific community. In order to do so, results must be highly consistent and require little to no user manipulation to achieve high quality nynerical results that can be combined with other imaging modalities.

关键词： phase imaging interference microscopy polarization interferometry cellular imaging cell dynamics optical thickness measurement

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Selective plane illumination microscopy with structured illumination based on spatial light modulators

Selective plane illumination microscopy with structured illu...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Li, Runze Zhou, Xing Wu, Di Peng, Tong Yang, Yanlong Lei, Ming Yu, Xianhua Yao, Baoli Ye, Tong Chinese Acad Sci Xian Inst Opt & Precis Mech State Key Lab Transient Opt & Photon Xian Shaanxi Peoples R China Clemson Univ Ctr Opt Mat Sci & Engn Technol Dept Bioengn Clemson SC 29631 USA

ISBN: (纸本)9780819498625

Structured-illumination microscopy (SIM) is an efficacious tool to decrease the contribution of the out-of-focus light to images of specimens. However, in SIM, the frequency of the spatial modulation applied to specimens should be adjustable according to the optical properties of the specimens to reach the optimal contrasts. Hence, a common theme in SIM is how the flexibility and quality of modulations at different frequencies can be improved. Digital scanned laser light-sheet microscopy with structured illumination (DSLM-SI) has been the most flexible means for generating modulation and optical sectioning. The complexity of synchronization between the temporal modulation and the beam scanning makes it hard to use and less stable;it also takes more time to acquire images for one plane than selective plane illumination microscopy (SPIM). In this report, we present a recent effort to use a spatial light modulator (SLM) to provide spatial modulation in SPIM. With the SLM, both of the frequency and phase of lateral modulation can be changed rapidly;moreover, this SLM-based SPIM can achieve fast imaging without mechanical moving parts.

关键词： Structured-illumination light-sheet spatial light modulator digital scanned laser light-sheet single-plane illumination

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Portable advanced off-axis camera for quantitative phase microscopy

Portable advanced off-axis camera for quantitative phase mic...

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Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XXI

作者： Monemhaghdoust, Z. Depeursinge, C. Moser, C. Ecole Polytech Fed Lausanne Lab Appl Photon Devices CH-1015 Lausanne Switzerland Lyncee Tec SA CH-1015 Lausanne Switzerland

ISBN: (纸本)9780819498625

We propose and experimentally demonstrate a device in which common-path interferometry combined with off-axis holographic geometry is used to realize a digital holographic camera which can be attached to the camera port of a conventional transmission microscope for complex wavefront analysis. A thick transmission volume grating recorded holographically into thick photosensitive glass splits the beam containing the sample information in two beams. The untouched transmitted beam creates the sample arm of the interferometer. The Bragg diffracted order of the grating is spectrally and spatially filtered by diffraction to generate a clean reference beam. Double passing the diffracted order through the grating using a retroreflector device provides filtering in two dimensions. The spatial filtering done by the grating which works based on high angular selectivity of thick volume gratings, reduces the alignment spatial sensitivity which is an advantage over the conventional spatial filtering done by pinholes. Besides, using a second thick grating, we introduce a desired coherence plane tilt in the reference beam which is sufficient to create high-visibility interference over the entire field of view. The full-field off-axis interferograms are created from which the amplitude and phase can be reconstructed. The advantage of the proposed camera is the insensitivity to the alignment, thus can be the basis for a standalone camera mountable on a standard optical microscope.

关键词： Diffraction gratings Digital holographic microscopy Portable camera Off-axis full-field imaging Phase microscopy

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three-dimensional and multidimensional microscopy: image acquisition and processing XXI

Three-Dimensional and Multidimensional Microscopy: Image Acq...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXI

ISBN: (纸本)9780819498625

The proceedings contain 40 papers. The topics discussed include: study of self-interference incoherent digital holography for the application of retinal imaging;further developments in addressing depth-variant 3D fluorescence microscopy imaging;flash extreme ultraviolet holographic microscopy in a table top setup;compressive sensing spectral domain optical coherence tomography with dispersion compensation;the Gray Institute open microscopes applied to radiobiology and protein interaction studies;high-contrast 3D microscopic imaging of deep layers in a biological medium;analysis of phase conjugation in a turbid medium;a model for ultrasound modulated light in a turbid medium;quantifying melanin distribution using pump-probe microscopy and a 2D morphological autocorrelation transformation for melanoma diagnosis;numerical spherical aberration correction method using spatial light modulator under deep-part fluorescence observation;and wavefront shaping of a Bessel light field enhances light sheet microscopy with scattered light.

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