检索结果-内蒙古大学图书馆

Conference on 3 dimensional and multidimensional microscopy - image acquisition processing VII

作者： Sugiyama, J Ogawa, Y Natl Food Res Inst Tsukuba Ibaraki 3058642 Japan

ISBN: (纸本)081943535X

In order to visualize sugar content of a melon, the relationship between sugar content and absorption spectra was investigated using a near-infrared (NIR) spectrometer. The absorbance at 676nm, which is close to the chlorophyll absorption band, had a high inverse correlation with sugar content. A high-resolution cooled CCD imaging camera with the band-pass filter of 676nm was used to capture the spectral absorption image. The calibration method for converting the absorbance on the image into Brix sugar content was developed in accordance with MR techniques. Applying this method to each pixel of the absorption image, a color distribution map of the sugar content was constructed. In addition, a special slicing device that can cut a melon in each 5mm thickness was developed in order to create a 3-D image of sugar content distribution.

关键词： three-dimensional near infrared spectral image sugar content melon

来源：评论

学校读者我要写书评

暂无评论

Pupil Polarimetry using Stress-Engineered Optical Elements

Pupil Polarimetry using Stress-Engineered Optical Elements

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XVII

作者： Beckley, Amber M. Brown, Thomas G. Univ Rochester Inst Opt Rochester NY 14627 USA

ISBN: (纸本)9780819479662

Stress-engineered optical elements show fascinating and potentially useful effects when placed at the pupil plane of an imaging system. When illuminated by a beam of spatially uniform polarization, a snapshot (single measurement) polarimetry method can be constructed. We expand upon this method to perform snapshot pupil polarimetry for spatially varying pupil polarization. We present the theory for snapshot non-uniform pupil polarization measurement using a stress-engineered optical element, as well as simulation results.

关键词： Polarimetry microscopy Cylindrical Vector Beams

来源：评论

学校读者我要写书评

暂无评论

Lens-less holographic microscopy with high resolving power for 4D measurement of microorganism swimming in water

Lens-less holographic microscopy with high resolving power f...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XIX

作者： Otani, Mayu Sato, Kunihiro Univ Hyogo Dept Elect Eng & Comp Sci Grad Sch Engn Himeji Hyogo 6712201 Japan

ISBN: (纸本)9780819488701

A lens-less holographic microscopy is developed for observation of high-resolution 4-D (spatio-temporal) images. A complex-amplitude in-line hologram with a large numerical aperture is extracted from a large off-axis hologram by applying one-shot digital holography. An angular spectrum method is developed for fast numerical reconstruction of precise 3-D image from the in-line hologram with a large numerical aperture. 3-D intensity and phase images with no distortion, with a resolution higher than 1 mu m, and with a depth larger than 1mm are recorded and reconstructed by the present holographic microscopy. Possibility is demonstrated to realize 4-D measurement of microorganisms swimming in water.

关键词： lens-less holographic microscopy 4-D measurement microorganism phase measurement

来源：评论

学校读者我要写书评

暂无评论

High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera

High speed slit-scanning confocal laser microscopy with an a...

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XII

作者： Han, SM Im, KB Park, H Kim, D Kim, BM Yonsei Univ Dept Biomed Engn Kangwon Do 220710 South Korea

ISBN: (纸本)0819456756

Using an acousto-optic beam deflector and a line scan camera, we constructed a non-mechanical, slit-scanning confocal microscope. It generates two-dimensional 512x512 images with speed of 60 frames/sec, which can be easily expanded, to 100 frames/sec. The measured axial and lateral resolutions of the system are 3.3 mu m and < 1 mu m, respectively, with a 50X objective. This simple design can produce frames rates faster than other commercial products with comparable resolution, which may be useful for analysis of rapid interactions in various biological applications.

关键词： confocal microscope three-dimensional microscopy acousto-optic deflector line scan CCD

来源：评论

学校读者我要写书评

暂无评论

A comparison of methods for optical sectioning using structured illumination microscopy

A comparison of methods for optical sectioning using structu...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Thomas, Benjamin Momany, Michelle Kner, Peter Univ Georgia Coll Engn Athens GA 30602 USA Univ Georgia Dept Plant Biol Athens GA 30602 USA

ISBN: (纸本)9780819493583

Structured Illumination microscopy is a simple and effective method to remove out-of-focus light in widefield fluorescence microscopy. Neil et al. originally proposed a simple square-law method for calculating the optically sectioned image from the three raw images with the structured illumination pattern super-imposed. However, the Neil method does not make the most efficient use of the three raw images. The three structured illumination images can also be used to separate three copies of the image covering shifted regions of frequency space in a similar manner to that developed by Gustafsson et al. These can then be combined using a generalized Wiener filter to create an image with a well-behaved optical transfer function in which the missing cone has been filled in, providing optical sectioning. Here, we compare the Neil and Gustafsson methods and show that the Gustafsson method provides an image with higher fidelity and a better Signal to Noise Ratio (SNR) at low photon counts. We apply the two methods to images of fluorescent beads and GFP labeled septins in Aspergillus nidulans.

关键词： fluorescence microscopy structured illumination microscopy three-dimensional imaging bioimaging

来源：评论

学校读者我要写书评

暂无评论

Nano-scale optical imaging technique

Nano-scale optical imaging technique

引用

Conference on 3-dimensional and multidimensional microscopy - image acquisition and processing XI

作者： Wu, S Tang, YC PEER Ctr Covina CA 91722 USA

ISBN: (纸本)0819452327

The optical resolution limits the observation of fine features with size below diffraction limit, e.g. 500nm. We propose and demonstrate a technique that uses collimated laser scattering to characterize such features. We use a new illumination technique that let us image bio-molecules down to 50nm with optical microscopy. We observed vesicles with size down to 50nm and visualize the flow of such tiny molecules in the live cells. We could also characterize the detailed size and molecular weight of such particles.

关键词： single particle laser scattering microscopy

来源：评论

学校读者我要写书评

暂无评论

A compact polarization converter to observe molecular orientation

A compact polarization converter to observe molecular orient...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XIV

作者： Hashimoto, Mamoru Yoshiki, Keisuke Kanamaru, Ryosuke Hashimoto, Nobuyuki Araki, Tsutomu Osaka Univ Grad Sch Engn Sci 1-3 Machikaneyama Toyonaka Osaka 5608531 Japan Citizen Watch Co Ltd MHT R&D Dept Tokorozawa Saitama 3598511 Japan

ISBN: (纸本)9780819465566

We developed a compact polarization-converter using two liquid-crystal spatial-light-modulators with eight electrodes. The converter converted a linearly polarized beam to two orthogonal linearly polarized beams and a radially polarized beam, and the direction of the electric filed at the focal point were controlled three-dimensionally. We constructed a second-harmonic-generation microscope using the polarization-converter to observe three-dimensional molecular orientation and demonstrated the detectability of molecular orientation.

关键词： molecular orientation nonlinear microscopy second-harmonics-generation pupil filtering radially polarized beam

来源：评论

学校读者我要写书评

暂无评论

A new expanded point information content design approach for 3D live-cell microscopy at video rates

A new expanded point information content design approach for...

引用

Conference on three-dimensional and multidimensional microscopy - image acquisition and processing XX

作者： Zahreddine, Ramzi N. Cormack, Robert H. Cogswell, Carol J. Univ Colorado Dept Elect Comp & Energy Engn Boulder CO 80309 USA

ISBN: (纸本)9780819493583

Through a combination of optical design and algorithm development, a new expanded point information content ( EPIC) microscope has been developed that is capable of extending the depth of field while simultaneously super locating the depth position of complex biological objects to within an accuracy of 75 nm. The data is then combined to form 3D animations of live-cell biological specimens. This is accomplished without the need to acquire multi-focal image stacks and is thus well suited for high-speed imaging.

关键词： 4D microscopy PSF engineering extended depth of field quantitative depth ranging computational optics live-cell imaging fluorescence microscopy

来源：评论

学校读者我要写书评

暂无评论

Transparent three-dimensional microstructures for trapping of living cells

引用

Proceedings of SPIE - The International Society for Optical Engineering 1999年 3605卷 241-249页

作者： Otte, K. Zimmer, K. Zeitschel, U. Hirsch, D. Reichenbach, A. Bigl, F. Bigl, V. Inst fuer Oberflaechenmodifizierung e.V. Leipzig Germany

ISBN: (纸本)0819430757;9780819430755

A combination of e-beam lithography (EBL), laser ablation, reactive ion beam etching (RIBE) and different wet etching techniques was used to fabricate different transparent three-dimensional structures in a three-layer system (Si3N4, SiO2, Si3N4) on quartz specially sized for the trapping of living neural cells under physiological conditions. The resulting structures show potential for trapping of cells, the stabilization of the cell membrane and the precise placement of the cells for a multimode of biological investigations.

关键词： Atomic force microscopy

来源：评论

学校读者我要写书评

暂无评论

Proceedings of SPIE: three- dimensional and multidimensional microscopy - image acquisition and processing

Proceedings of SPIE: Three- Dimensional and Multidimensional...

引用

three-dimensional and multidimensional microscopy:image acquisition and processing X

The proceedings contains 20 papers from the conference on three-dimensional and multidimensional microscopy: image acquisition and processing X. The topics discussed include: high-resolution imaging of biological samples;benifits and applications of programmable spectral devices in confocal microscopes;logitudinal field imaging and confocal endoscopy.

关键词： Medical imaging

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：