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three-dimensional and multidimensional microscopy: image acquisition and processing xxx

Three-Dimensional and Multidimensional Microscopy: Image Acq...

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three-dimensional and multidimensional microscopy: image acquisition and processing xxx 2023

ISBN: (纸本)9781510658752

The proceedings contain 13 papers. The topics discussed include: compressive time-resolved multispectral fluorescence microscopy with single-pixel camera;optical sectioning microscopy with structured illumination and single-pixel detection;high-speed light-sheet microscopy imaging and data post-processing using custom hardware and software solutions;utilizing fluorescent beads to mimic single fluorophores;single-shot capture bright-field and digital holographic microscopy for the analysis of living cells;simulations of imperfect refractive index matching in scanning laser optical tomography and a method for correction;super-resolution in confocal microscopy using generative adversarial networks with paired and unpaired data;estimation of 3D point spread function of a widefield microscope by stochastic least square minimization method;and design and implementation of plenoptic imaging system with reduced aberrations.

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three-dimensional and multidimensional microscopy: image acquisition and processing xxxI

Three-Dimensional and Multidimensional Microscopy: Image Acq...

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three-dimensional and multidimensional microscopy: image acquisition and processing xxxI 2024

ISBN: (纸本)9781510669550

The proceedings contain 14 papers. The topics discuss include: light-field microscopy to study cardiac arrhythmias in zebrafish larvae;on-the-fly deconvolution of diagonally-scanned airy lightsheet microscopy;image projection through a multimode fiber with reference-lessly retrieved transmission matrix;design and fabrication of universal resolution targets for microsphere-assisted microscopy;normalized level set model for segmentation of low-contrast objects in 2- and 3- dimensional images;development of long axial range 3D single-particle tracking by PSF engineering using birefringent substrates;Chernoff information for creating maximally distinctive engineered point spread functions for multi-color single molecule imaging;and hyperspectral microscopy as a particle exposure assessment tool.

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three-dimensional and multidimensional microscopy: image acquisition and processing XXIX

Three-Dimensional and Multidimensional Microscopy: Image Acq...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXIX 2022

ISBN: (纸本)9781510648036

The proceedings contain 12 papers. The topics discussed include: high-resolution volumetric imaging using hybrid image of Fourier light-field microscopy and wide-field microscopy;quantification of collagen networks in mammary tumors using TPEF and laser-based tomography;low dosage 3D volume fluorescence microscopy imaging using compressive sensing;multispectral time-resolved fluorescence microscopy based on compressive acquisitions;a compact open-top light-sheet microscope for optofluidic imaging;validation of excitation-scan hyperspectral multi-faceted mirror array prototype system advancements to hyperspectral imaging applications;efficient and versatile aberration correction through sensorless adaptive optics;and hyperspectral imaging and adaptive thresholding to identify agonist-induced camp signals in pulmonary microvascular endothelial cells.

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three-dimensional and multidimensional microscopy: image acquisition and processing XXVIII

Three-Dimensional and Multidimensional Microscopy: Image Acq...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXVIII 2021

ISBN: (纸本)9781510641334

The proceedings contain 15 papers. The topics discussed include: sparsity-based approach for 3D super-resolution microscopy from correlation information of high emitter-density frames;quantitative phase imaging-based machine learning approaches for the analysis of adherent and suspended cells;automatic nuclei segmentation based on fuzzy C- mean;evaluation of different illumination designs for tunable 3D structured illumination microscopy through model-based restoration;high-throughput 3D imaging with compressive lens-free tomography;intensity uniformity optimization in spatial-light-modulator-based multifocal microscope;and method for measurement of collagen monomer orientation in fluorescence microscopy.

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Optical sectioning microscopy with structured illumination and single-pixel detection 30

Optical sectioning microscopy with structured illumination a...

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three-dimensional and multidimensional microscopy: image acquisition and processing xxx 2023

作者： Ordóñez, Luis Ipus, Erick Lenz, Armin J.M. Lancis, Jesús Tajahuerce, Enrique Universitat Jaume I Castelló12071 Spain

ISBN: (纸本)9781510658752

We propose a new single pixel microscope with optical sectioning properties by using structured illumination techniques. The method uses single-pixel imaging (SPI) techniques by interrogating the sample with a series of spatially resolved patterns and measuring the output intensities with a non-spatial resolution detector. Moreover, optical sectioning is obtained by adding a grating to the system and employing structured illumination microscopy (SIM). The system allows us to perform 3D bright-field and fluorescence microscopy. We apply compressive sensing techniques to decrease the acquisition time. © 2023 SPIE. All rights reserved.

关键词： Fluorescence microscopy

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Super-resolution in confocal microscopy using generative adversarial networks with paired and unpaired data 30

Super-resolution in confocal microscopy using generative adv...

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three-dimensional and multidimensional microscopy: image acquisition and processing xxx 2023

作者： Patra, S. Trujillo, C. Doblas, A. Department of Electrical and Computer Engineering The University of Memphis TN United States School of Applied Sciences and Engineering Universidad EAFIT Medellin Colombia

ISBN: (纸本)9781510658752

The advantages of confocal microscopy over widefield microscopy are their ability to produce optically sectioned images and their ability to produce multi-color imaging in which different organelles within the biological specimen are stained using multiple dyes, enabling colocalization studies. These features make confocal microscopy a widely used tool to provide valuable morphological and functional information within cells and tissues. One of the major drawbacks of confocal microscopy is its limited spatial resolution. Here, we train two generative adversarial networks using paired and unpaired data of low- and high-resolution images to improve the spatial resolution in confocal microscopy. © 2023 SPIE. All rights reserved.

关键词： Confocal microscopy

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Utilizing fluorescent beads to mimic single fluorophores 30

Utilizing fluorescent beads to mimic single fluorophores

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three-dimensional and multidimensional microscopy: image acquisition and processing xxx 2023

作者： Feng, Sherry Yi-Ting Alemán-Castañeda, Luis A. Gutiérrez-Cuevas, Rodrigo Herrera, Isael Brown, Thomas G. Brasselet, Sophie Alonso, Miguel A. The Institute of Optics University of Rochester RochesterNY14627 United States Aix Marseille Univ CNRS Centrale Marseille Institut Fresnel MarseilleF-13013 France Institut Langevin ESPCI Paris Université PSL CNRS Paris75005 France

In this paper, numerical and semi-analytical methods are used for studying how well a fluorescent bead with a given radius in a polarizer-supplemented microscopy setup, can emulate a single fluorescent molecule. ©... 详细信息

ISBN: (纸本)9781510658752

关键词： Fluorescence microscopy

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High-speed light-sheet microscopy imaging and data post-processing using custom hardware and software solutions 30

High-speed light-sheet microscopy imaging and data post-proc...

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three-dimensional and multidimensional microscopy: image acquisition and processing xxx 2023

作者： Mazzamuto, Giacomo Silvestri, Ludovico Sancataldo, Giuseppe Gavryusev, Vladislav Scardigli, Marina Costantini, Irene Pavone, Francesco S. Sesto Fiorentino Italy Sesto Fiorentino Italy Department of Physics and Astronomy University of Florence Italy Department of Physics and Chemistry University of Palermo Italy Department of Biology University of Florence Italy

ISBN: (纸本)9781510658752

High-speed imaging with Light-Sheet Fluorescence microscopy poses several challenges throughout the whole pipeline, from data acquisition in the lab to image stitching and post-processing. Here we present our custom hardware and software solutions that allow us to map large biological samples at the cellular level, e.g. large portions of human brain cortex. Our custom optical setup — a dual-view, inverted, light-sheet microscope — is capable of simultaneous two-color acquisition at a data rate of 1 GB/s. Our open source tools include the instrument’s data acquisition and control software and also cover volumetric image stitching and post-processing. © 2023 SPIE. All rights reserved.

关键词： Brain

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three-dimensional and multidimensional microscopy: image acquisition and processing XXVII

Three-Dimensional and Multidimensional Microscopy: Image Acq...

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three-dimensional and multidimensional microscopy: image acquisition and processing XXVII 2020

ISBN: (纸本)9781510632530

The proceedings contain 14 papers. The topics discussed include: multi-immersion open-top light-sheet microscopy;compact spectrograph for hyperspectral imaging with overlapping fluorescent reporters;computational super-resolution microscopy: leveraging noise model, regularization and sparsity to achieve highest resolution;wavelet-based characterization of the spatial relationship of nerve and collagen in neuropathic adipose tissue;multifocus imaging via polarization wavefront shaping;a study for accelerating the speed of all-in-focus image processing;excitation-scan mirror array system advancements to hyperspectral imaging applications;and 3D refractive index tomography with quantitative oblique back-illumination microscopy.

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Single-shot capture bright-field and digital holographic microscopy for the analysis of living cells 30

Single-shot capture bright-field and digital holographic mic...

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three-dimensional and multidimensional microscopy: image acquisition and processing xxx 2023

作者： Picazo-Bueno, José Ángel Barroso, Álvaro Ketelhut, Steffi Schnekenburger, Jürgen Micó, Vicente Kemper, Björn Biomedical Technology Center University of Muenster Mendelstraβe 17 MuensterD-48149 Germany Department of Optics and Optometry and Vision Sciences University of Valencia C/Doctor Moliner 50 Burjassot46100 Spain

ISBN: (纸本)9781510658752

Digital holographic microscopy (DHM) is an emerging label-free modality for quantitative imaging of semi-transparent biological specimens. DHM provides quantitative phase imaging (QPI) of the light transmitted or scattered by an object, which allows to extract information about morphology and dynamics of investigated specimens. DHM can be implemented with other optical microscopy techniques like bright field (BF) or fluorescence, for example, to provide complementary information about cellular processes. Multimodal microscopy is often achieved by sequential acquisition of information from different imaging modalities or simultaneously by capturing images with different acquisition devices. However, such arrangements can be cost-intensive and/or limited in speed for imaging of dynamic processes. Here, we investigate a single-snapshot multimodal approach that enables simultaneous acquisition of DHM-based QPI and BF images and is compatible with common optical microscopes for the analysis of living cells. The technique is based on spatially multiplexed interferometric microscopy (SMIM), where off-axis holograms are generated by spatial multiplexing of the illuminated sample plane utilizing a diffraction grating, which is extended by additional superposition of complementary white-light image information. image separation is achieved by Fourier-transformation-based numerical demultiplexing procedures. The technique is firstly characterized by investigations on microspheres. Then, the application on living adherent and suspended cells is illustrated. © 2023 SPIE. All rights reserved.

关键词： Holograms

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