目的:试图从上尿路结石中分离纳米细菌(Nanobacteria,NB)并通过电镜鉴定,以判断纳米细菌在上尿路结石中是否存在。
材料与方法:17枚上尿路结石分别由肾盂、肾实质以及输尿管切开取出或用输尿管镜取出,所有结石均经处理后在细胞培养条件下培养4-5周后通过相差显微镜、透射电镜(transmission electron microscope,TEM)、扫描电镜观察(Scanning electron microscope,SEM)。用Hoechst33258进行纳米细菌DNA染色,于荧光显微镜下观察。
结果:17枚结石培养3周后可于相差显微镜下见纳米细菌,4周后更加明显。4-5周培养物于透射电镜及扫描电镜下见纳米细菌。Hoechst33258染色DNA后荧光显微镜下见纳米细菌着色微弱,与对照无显著区别。
结论:纳米细菌在上尿路结石中存在,可能在结石形成中起作用
The effects of random long-range connections （shortcuts） on the transitions of neural firing patterns in coupled Hindmarsh-Rose neurons are investigated, where each neuron is subjected to an external current. It is ...
详细信息
The effects of random long-range connections （shortcuts） on the transitions of neural firing patterns in coupled Hindmarsh-Rose neurons are investigated, where each neuron is subjected to an external current. It is found that, on one hand, the system can achieve the transition of neural firing patterns from the fewer-period state to the multi-period one, when the number of the added shortcuts in the neural network is greater than a threshold value, indicating the occurrence of in-transition of neural firing patterns. On the other hand, for a stronger coupling strength, we can also find the similar but reverse results by adding some proper random connections. In addition, the influences of system size and coupling strength on such transition behavior, as well as the internality between the transition degree of firing patterns and its critical characteristics for different external stimulation current, are also discussed.
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