With slight modification of the candle jar method described by Trager and Jensen, Plasmodium falciparum has been successfully cultivated in vitro with fresh rabbit serum instead of human serum. Each 50 ml Erlenmeyer f...
With slight modification of the candle jar method described by Trager and Jensen, Plasmodium falciparum has been successfully cultivated in vitro with fresh rabbit serum instead of human serum. Each 50 ml Erlenmeyer flask containing 5 ml culture was incubated in a candle desiccator at 35.5-37 C. 15 ml rabbit serum was added to 100 ml RPMI 1640 medium with sodium bicarbonate. In the culture both infected and uninfected red cells were 5%.The culture has been maintained alive for more than 500 days and the infection rate was over 20%.Cultivation was also conducted in plastic dishes(3.5 cm in diameter) containing 1.5 ml of the culture, but using human serum instead of rabbit serum for comparison. The growth rate showed no significant *** results suggest that rabbit serum may be used with advantage instead of human serum for continuous culture of the malaria parasite.
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