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Time-dependent gut microbiota analysis of juvenile Oreochromis niloticus by dietary supplementation of resveratrol

ARCHIVES OF MICROBIOLOGY 2020年第1期202卷 43-53页

作者： Zheng, Yao Hu, Gengdong Wu, Wei Qiu, Liping bing, xuwen Chen, Jiazhang Chinese Acad Fishery Sci Fishery Ecoenvironm Monitoring Ctr Lower Reaches Freshwater Fisheries Res Ctr Minist AgrLab Qual & Safety Risk Assessment Aqua 9 Shanshui East Rd Wuxi 214081 Jiangsu Peoples R China Minist Agr Key Lab Control Qual & Safety Aquat Prod Beijing 100141 Peoples R China

To evaluate the changes in bacterial diversity at various time points under resveratrol supplementation, we aimed to investigate the diversification of gut microbiota and the changes in total genetic diversity. We performed 16S rDNA gene sequencing at different time points (15, 30, and 45 days) to analyze the gut microbiota of tilapia. Fusobacteria, Proteobacteria, and Bacteroidetes (15 days) or Cyanobacteria (30 and 45 days) were found to be the three most abundant phyla. Cyanobacteria (15 and 30 days), Proteobacteria (15 days), Firmicutes and Chlamydiae (30 and 45 days), Planctomycetes (30 days), Bacteroidetes, Actinobacteria, and Fusobacteria (45 days) in the 0.05 g/kg RES group increased as compared to that in the controls. Proteobacteria and Cyanobacteria significantly decreased and increased at 30 and 45 days, respectively, while the reverse pattern was observed at 15 days. The Bacteroidetes:Firmicutes and Proteobacteria:Cyanobacteria ratios were significantly increased (15 and 45 days, P < 0.05) and decreased (30 days, P < 0.05). RES supplementation did not affect the richness and diversity of the gut microbiota in tilapia. Our findings may contribute to the development of strategies for the management of diseases.

关键词： Resveratrol GIFT Gut microbiota 16S rDNA Chinese medical herbs

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白藜芦醇对红罗非鱼肠道组织学和炎症因子含量的影响

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动物营养学报 2022年第1期34卷 589-599页

作者：施羽露杨晓曦郑尧裘丽萍胡庚东邴旭文陈家长南京农业大学无锡渔业学院无锡214081 中国水产科学研究院淡水渔业研究中心/农业农村部长江下游渔业生态环境监测中心/农业农村部水产品质量安全环境因子风险评估实验室无锡214081

本试验旨在探究饲料中添加白藜芦醇(Res)对高脂摄食红罗非鱼生长性能、腹脂沉积、肠道组织结构及炎症因子含量的影响。选用红罗非鱼360尾,设置8%脂肪(A组)、10%脂肪(B组)、10%脂肪+Res(C组)以及10%脂肪+Res+抑制剂EX527(D组)4个组,每组... 详细信息

本试验旨在探究饲料中添加白藜芦醇(Res)对高脂摄食红罗非鱼生长性能、腹脂沉积、肠道组织结构及炎症因子含量的影响。选用红罗非鱼360尾,设置8%脂肪(A组)、10%脂肪(B组)、10%脂肪+Res(C组)以及10%脂肪+Res+抑制剂EX527(D组)4个组,每组3个重复,每个重复30尾,试验期为63 d。分别在第3、6、9周测定末体重,并采集中肠组织、肠道内容物和血清,测定炎症因子及脂多糖(LPS)含量。结果表明:与A组相比,B组体重、增重率和特定生长率显著升高(P<0.05),且第3、6周腹脂率显著升高(P<0.05),第9周中肠组织白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)含量显著升高(P<0.05),中肠组织转化生长因子-β(TGF-β)含量显著降低(P<0.05);血清LPS含量显著升高(P<0.05),肠道内容物LPS含量随时间呈上升趋势。与B组相比,C组体重、增重率、特定生长率、饲料系数显著降低(P<0.05);血清LPS含量显著降低(P<0.05),且肠道内容物LPS含量随时间呈下降趋势;第9周中肠组织IL-1β、白细胞介素-6(IL-6)和TNF-α含量显著降低(P<0.05),TGF-β含量显著升高(P<0.05)。B组肠道环状皱襞均存在破损现象,C组肠道杯状细胞较B组和D组有所增加。综上所述,添加Res可通过降低LPS含量、提高杯状细胞数量缓解因高脂饲料导致的腹腔脂肪过度沉积、肠道炎症及组织病变,以调控鱼生长,有效保护肠道健康。

关键词：白藜芦醇红罗非鱼肠道炎症因子肠道显微结构

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Varieties of immunity activities and gut contents in tilapia with seasonal changes

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FISH & SHELLFISH IMMUNOLOGY 2019年 90卷 466-476页

作者： Zheng, Yao Wu, Wei Hu, Gengdong Qiu, Liping bing, xuwen Chen, Jiazhang Chinese Acad Fishery Sci Freshwater Fisheries Res Ctr 9 Shanshui East Rd Wuxi 214081 Jiangsu Peoples R China

We performed 16S rDNA sequencing of tilapia fecal samples to analyze changes in tilapia gut contents after cultivation of the fish in the presence of sandwich-like floating beds of Chinese medicinal herbs (5 and 10% planting-areas;5% Polygonum cuspidatum). The interactive effects between water quality and blood and hepatic pro- and anti-inflammatory concentrations were also assessed. Our results showed that the water quality (i.e., NO3--N, NO2--N, TP removal rates) improved, and the abundance of Chloroflexi and Cyanobacteria increased. The abundance of Bacteroidetes, Verrucomicrobia, Saccharibacteria, and Actinobacteria showed both significant seasonal decreases and increases in the presence of P. cuspidatum (increases in August and decreases in July). Fish blood and hepatic IL-10 and IFN-gamma levels (together with fish sampled in September) significantly increased in the P. cuspidatum group sampled in August, while those of TNF-alpha (10% sandwich-like, P. cuspidatum), IL-1 beta (P. cuspidatum), IL-8 (5% sandwich-like in September, S905S) significantly decreased. Heat shock proteins 60 and 70 levels significantly increased in the P. cuspidatum group, and complement C3 and C4 concentrations significantly increased in S905S. This study demonstrated that enhanced immunity through the regulation of pro- and anti-inflammatory proteins was sustained throughout development until harvest, particularly in fish grown with P. cuspidation.

关键词： Tilapia Gut contents Floating bed cultivation 16S rDNA Disease

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氨氮胁迫对翘嘴鳜幼鱼抗氧化酶、消化酶活性及应激相关基因表达的影响

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水产科技情报 2024年第1期51卷 44-51页

作者：孙阿君丁炜东曹丽萍曹哲明邴旭文中国水产科学研究院淡水渔业研究中心农业农村部淡水渔业和种质资源利用重点实验室无锡214081

为了解氨氮胁迫下翘嘴鳜(Siniperca chuatsi)幼鱼肝脏、胃抗氧化系统和消化系统的响应机制,以体质量为(15.27±0.67)g的翘嘴鳜幼鱼为研究对象,探讨了6个氨氮胁迫质量浓度(0、10、20、30、40、50 mg/L)下肝脏和胃中谷胱甘肽(GSH)、... 详细信息

为了解氨氮胁迫下翘嘴鳜(Siniperca chuatsi)幼鱼肝脏、胃抗氧化系统和消化系统的响应机制,以体质量为(15.27±0.67)g的翘嘴鳜幼鱼为研究对象,探讨了6个氨氮胁迫质量浓度(0、10、20、30、40、50 mg/L)下肝脏和胃中谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、脂质过氧化物(LPO)、胃蛋白酶(pepsin)、胃淀粉酶(AMS)、胃脂肪酶(LPS)的活性和IL-1β、IL-8、TNF-α、HSP90α等应激相关基因的表达情况。试验结果表明,随着氨氮质量浓度的提高,肝脏抗氧化相关酶中的SOD、CAT、GSH活性呈先升高后下降的趋势,LPO活性呈逐渐升高的趋势,IL-1β、HSP90α基因表达量呈先升高后降低的趋势,TNF-α表达量呈逐渐升高的趋势,IL-8表达量呈降低-升高-降低的趋势;胃消化酶中的胃蛋白酶活性呈现先升高后降低的趋势,而AMS和LPS活性呈逐渐升高的趋势。结果表明,在低浓度氨氮胁迫条件下,鱼体通过诱导抗氧化酶活性升高和应激相关基因表达上调来应对氧化应激损伤,同时诱导升高胃消化酶活性为机体提供能量,而高浓度氨氮胁迫则抑制了抗氧化酶活性和HSP90α基因的表达。

关键词：氨氮胁迫翘嘴鳜抗氧化酶消化酶炎症相关基因酶活力

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Reshaping fecal gut microbiota composition by growing with Polygonum cuspidatum, Houttuynia cordata, and Ipomoea aquatica

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CANADIAN JOURNAL OF MICROBIOLOGY 2019年第7期65卷 522-529页

作者： Zheng, Yao Hu, Gengdong Wu, Wei Qiu, Liping Li, Dandan bing, xuwen Chen, Jiazhang Chinese Acad Fishery Sci Freshwater Fisheries Res Ctr Wuxi Jiangsu Peoples R China

We carried out sequencing of samples cultivated in floating beds with different Chinese medicinal herbs (Control, Houttuynia cordata Thunb., Polygonum cuspidatum, and a combination of H. cordata with Ipomoea aquatica Forssk.;named groups A, B, C, D, respectively) to analyze changes in the composition of gut microbiota of tilapia feces. Fusobacteria (ranging from 49.0% to 73.3%), Firmicutes (12.3%-37.8%), and Proteobacteria (5.1%-23.0%) were found to be the most dominant phyla present in all samples. The operational taxonomic units and the Ace and Chao1 indices of groups A and D were significantly higher than those of group C. Polygonum cuspidatum decreased the species richness and diversity of microbial communities in tilapia intestinal feces. The phylum WCHB1-60, order Enterobacteriales, and genus Plesiomonas significantly decreased (in group A);the species Plesiomonas shigelloides significantly decreased (in groups B and C);and the genus Leucobacter significantly increased (in group D) when compared with the control. The relative abundance of the class Verrucomicrobiae (groups B vs C) significantly decreased. In the presence of I. aquatica, the phylum Bacteroidetes significantly decreased, while the genera Leucobacter and Pelotomaculum significantly increased. The ratio of Bacteroidetes to Firmicutes was significantly higher in groups B and C relative to the controls, while it decreased significantly in group D. The algae (i. e., Anabaena and Microcystis) and beneficial pathogenic bacteria decreased in groups C and D, respectively. In addition, Enterovibrio decreased in all treatment groups. The present data demonstrate that floating bed cultivation with Chinese medicinal herbs significantly alters the gut microbiota of tilapia, which may enhance its immune activity.

关键词： tilapia fecal gut microbiota floating bed cultivation 16S rDNA disease

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Benchmarks for permanent carbon in low-carbon probabilistic design of concrete structures: A case study of China

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JOURNAL OF CLEANER PRODUCTION 2025年 489卷

作者： Guan, Xiangshuo Xiao, Jianzhuang Xia, bing Xiao, xuwen Noguchi, Takafumi Tongji Univ Coll Civil Engn Dept Struct Engn 1239 Siping Rd Shanghai 200092 Peoples R China Guangxi Univ Inst Sci & Technol Carbon Peak & Neutral Nanning 530004 Peoples R China Hong Kong Polytech Univ Dept Civil & Environm Engn Hong Kong Peoples R China Chinese Acad Engn Beijing 100088 Peoples R China Univ Tokyo Grad Sch Engn Dept Architecture Bldg Mat Engn Lab Tokyo 1138654 Japan

China currently accounts for over 25% of global anthropogenic carbon emissions. Cutting down the carbon emissions of the construction industry is a major task for achieving the carbon neutrality goal in China. However, research on benchmarks and probability distribution models for China's embodied carbon emissions remains insufficient. To address this gap, this study defined "permanent carbon" by distinguishing the low time- variability part of embodied carbon emissions in buildings. Based on samples of 114 buildings in China, statistical and regression methods were utilized to construct a prior probability distribution model for permanent carbon of concrete structures, considering differences in building types. By applying Bayesian updating methods, posterior distributions were obtained based on latest region-specific information, addressing the issue of outdated data over time. Taking the East China region as an example, we found that the regional adjustment significantly affected carbon emission estimates, with varying adjustment factors for different building types. Transportation carbon emission benchmarks were influenced by transportation modes and distances, while distribution types remained stable. This study employs the Bayesian updating method in a novel way to help establish carbon emission benchmarks, support the formulation of carbon reduction targets, and aid the low carbon design during the early stage of building projects.

关键词： Concrete structures Permanent carbon Probability distribution model Carbon benchmark Regional adjustment

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氨氮胁迫对翘嘴鳜幼鱼鳃、消化道酶活力的影响

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南方水产科学 2020年第3期16卷 31-37页

作者：丁炜东曹丽萍曹哲明邴旭文中国水产科学研究院淡水渔业研究中心/农业农村部淡水渔业和种质资源利用重点实验室江苏无锡214081

为揭示翘嘴鳜(Siniperca chuatsi)幼鱼对氨氮胁迫的生理变化,以体质量(13.31±0.49)g、体长(9.73±0.46)cm的翘嘴鳜幼鱼为实验鱼,研究了在水温(22.0±0.5)℃下、48.65 mg·L-1氨氮胁迫对其鳃和消化道相关酶活力的影响... 详细信息

为揭示翘嘴鳜(Siniperca chuatsi)幼鱼对氨氮胁迫的生理变化,以体质量(13.31±0.49)g、体长(9.73±0.46)cm的翘嘴鳜幼鱼为实验鱼,研究了在水温(22.0±0.5)℃下、48.65 mg·L-1氨氮胁迫对其鳃和消化道相关酶活力的影响。结果表明,在氨氮胁迫下翘嘴鳜幼鱼处于应激状态并引起Na+/K+-ATP酶、呼吸代谢酶和消化酶活力水平显著变化。其中Na+/K+-ATP酶活力与对照组差异显著,先升高再降低;呼吸代谢酶活力呈升高趋势;对胃囊和消化道中的消化酶也有显著影响,其中淀粉酶(AMS)呈降低-升高的趋势,胃蛋白酶和脂肪酶(LPS)呈升高-降低的趋势。综上,氨氮胁迫会对翘嘴鳜幼鱼产生损害,使其呼吸代谢功能下降,鳃的呼吸功能受损,机体诱导消化酶与无氧代谢酶活力升高为机体抗应激反应提供能量。

关键词：翘嘴鳜幼鱼氨氮胁迫呼吸代谢酶消化酶酶活力

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虎杖对水质和微生物群落结构及功能的影响

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环境科学与技术 2020年第5期43卷 25-32页

作者：陈家长赵志祥施羽露史磊磊郑尧邴旭文中国水产科学研究院淡水渔业研究中心农业部长江下游渔业生态环境监测中心农业部水产品质量安全环境因子风险评估实验室(无锡)江苏无锡214081 南京农业大学无锡渔业学院江苏无锡214081

为研究虎杖种植后池塘水质变化和水体微生物群落碳源利用能力,该研究于室外构建了吉富罗非鱼养殖池塘5%虎杖生态浮床,2017年6-9月持续测定水质,利用Biolog技术分析池塘水体微生物群落结构及功能特征。结果表明6-7月生长期间虎杖种植能... 详细信息

为研究虎杖种植后池塘水质变化和水体微生物群落碳源利用能力,该研究于室外构建了吉富罗非鱼养殖池塘5%虎杖生态浮床,2017年6-9月持续测定水质,利用Biolog技术分析池塘水体微生物群落结构及功能特征。结果表明6-7月生长期间虎杖种植能有效削减池塘水体中TN、TP、Chl.a浓度,在温度较高的8、9月虎杖种植塘TP显著降低(P<0.05);7-9月虎杖种植塘水体微生物AWCD值显著增加(P<0.05),多样性McIntosh和Richness(除9月)指数均显著提高(P<0.05),且水体微生物对聚合糖类、氨基酸类、醇类的代谢能力显著提高(P<0.05)。在吉富罗非鱼养殖池塘种植虎杖,可在改善水质的基础上提高水体微生物群落多样性指数及对碳源的利用能力。

关键词：罗非鱼虎杖浮床微生物群落多样性碳源利用

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基于江苏省河蟹主产区气象因子的产量BP神经网络预测

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农学学报 2022年第6期12卷 50-54页

作者：金武何奇杜兴伟朱新艳闻海波马学艳何义进邴旭文中国水产科学研究院淡水渔业研究中心江苏无锡214081 常熟市水产技术推广站江苏常熟215500

为提高江苏河蟹主产区产量预测能力,以此为基础制定科学合理的河蟹养殖发展规划,本研究对河蟹主产区附近设立的7个观测台站2013—2017年的气象数据和该区产量数据进行了采集,利用插值法补足少量缺失值,通过主成分分析选取了最能代表江... 详细信息

为提高江苏河蟹主产区产量预测能力,以此为基础制定科学合理的河蟹养殖发展规划,本研究对河蟹主产区附近设立的7个观测台站2013—2017年的气象数据和该区产量数据进行了采集,利用插值法补足少量缺失值,通过主成分分析选取了最能代表江苏省河蟹主产区特点的5个主成分作为研究对象建立BP神经网络,探索其用于江苏省河蟹主产区产量随气象因子变化的规律。结果表明,通过PCA主成分分析降维处理后数据的大部分特点能被BP神经网络学习到,预测值与真实之间的相关系数为0.82267,具备一定的模拟气象因子数据与产量之间的关系的能力。

关键词：气象因子 BP神经网络河蟹产量短期预测

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吉富罗非鱼养殖池塘集成调控技术配比研究

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中国农学通报 2021年第33期37卷 148-152页

作者：孙旋辉蒋咏燚刘文婷郑尧陈家长邴旭文中国水产科学研究院淡水渔业研究中心南京农业大学无锡渔业学院江苏无锡214081

前期研究表明"5%空心菜+10%填料+泼藻"水质调控集成模式值得推广,为进一步验证效果,以吉富罗非鱼养殖池塘为对象,研究不同空心菜种植配比其对池塘水质氮、磷净化效果。通过设置5%、8%和10%空心菜种植比例,测定TN、NH;-N、TP、... 详细信息

前期研究表明"5%空心菜+10%填料+泼藻"水质调控集成模式值得推广,为进一步验证效果,以吉富罗非鱼养殖池塘为对象,研究不同空心菜种植配比其对池塘水质氮、磷净化效果。通过设置5%、8%和10%空心菜种植比例,测定TN、NH;-N、TP、CODMn、TSS、p H和DO等水质指标,并进行环境、效益评估。结果表明:空心菜种植对罗非鱼池塘水体中TN、NH;-N、TP、CODMn、TSS均有一定程度的降低,空心菜种植对TN去除率在10%~15%之间,对TP去除率在20%~40%之间,5%空心菜种植组还对NH;-N、CODMn有较好的去除效果,且效益较好适合推广。

关键词：池塘水质调控原位修复集成中草药鱼菜配比营养盐去除效果成本核算

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