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Histopathological analysis and the immune related gene expression profiles of mandarin fish (Siniperca chuatsi) infected with Aeromonas hydrophila

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FISH & SHELLFISH IMMUNOLOGY 2018年 83卷 410-415页

作者： Chen, Nan Jiang, Jingjing Gao, Xiaojian Li, Xixi Zhang, Yue Liu, Xiaodan Yang, Hui bing, xuwen Zhang, Xiaojun Yangzhou Univ Coll Anim Sci & Technol Yangzhou 225009 Jiangsu Peoples R China Chinese Acad Fishery Sci Freshwater Fisheries Res Ctr Key Lab Freshwater Fisheries & Germplasm Utilizat Minist Agr Wuxi 214081 Peoples R China

Hemorrhagic septicemia of mandarin fish (Siniperca chuatsi) was mainly caused by Aeromonas hydrophila which was an opportunistic pathogen. In recent years, the disease has caused tremendous economic loss with high morbidity and mass mortality in the mandarin fish breeding industry. Histopathological analysis and the immune related gene expression profiles of mandarin fish (S. chuatsi) infected with A. hydrophila were investigated in this study. Transmission electron microscopy (TEM) images showed that the cells of A. hydrophila densely covered with a mass of fimbriae. Histopathological analysis revealed that inflammation, vacuolization and extensive necrosis existed in the gill, liver, spleen and head kidney of the diseased fish. Quantitative real-time PCR was performed to measure mRNA expression levels for six immune related genes in mandarin fish after A. hydrophila infection. The transcriptional analysis of these immune related genes demonstrated that the expression levels of major histocompatibility complex class II (MHC II), T cell receptor alpha (TCR alpha), tumor necrosis factor alpha (TNF alpha), CC chemokine 3, interleukin 8 (IL-8) and Hepcidin were strongly up-regulated in spleen and head kidney of mandarin fish post-infection. These results will contribute to further study on the pathogenesis and host defensive system in A. hydrophila infection.

关键词： Mandarin fish Aeromonas hydrophila Histopathology Immune related genes

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The infection of red seabream iridovirus in mandarin fish (Siniperca chuatsi) and the host immune related gene expression profiles

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FISH & SHELLFISH IMMUNOLOGY 2018年 74卷 474-484页

作者： Liu, Xiaodan Chen, Nan Gao, Xiaojian Zhang, Yingying Li, Xixi Zhang, Yue bing, xuwen Huang, Hezhong Zhang, Xiaojun Yangzhou Univ Coll Anim Sci & Technol Yangzhou 225009 Jiangsu Peoples R China Chinese Acad Fishery Sci Freshwater Fisheries Res Ctr Minist Agr Key Lab Freshwater Fisheries & Germplasm Utilizat Wuxi 214081 Peoples R China Soochow Univ Fisheries Res Inst Sch Basic Med & Biol Sci Suzhou 215123 Peoples R China

Red Sea bream iridovirus (RSIV) was initially isolated from marine fish, which belongs to Megalocytivirus, Iridoviridae. It can cause great economic losses in fish culture with high morbidity and mortality. In the present study, the pathogenicity and immune response associated with a RSIV genotype megalocytivirus infection were determined in mandarin fish (Siniperca chuatsi). Fish challenged showed typical clinical signs of iridovirus infection, including acute haemorrhages and enlarged visceral organs. Histopathological analysis revealed that extensive necrosis, vacuolization and inflammation were presented in the stomach, spleen, kidney and liver of the diseased fish. Blood cells counting and phagocytic assay indicated that the numbers of the red and white blood cells in the peripheral blood of infected fish increased significantly compared to the control group and the phagocytic percentage of leukocytes peaked at day 6 post infection. Quantitive real-time PCR (qRT-PCR) was also undertaken to analyse the host defensive response in mandarin fish challenged with RSIV. The expression level of ten genes including interferon-related factors (IRFs) IRF1 and IRF7, Mx, Viperin, JAK1, STAT1, TCR alpha, TNF alpha, and IL-8 during experimental infection were monitored at different point of time in liver, spleen and head kidney. Results revealed varying expression profiles and clear transcriptional activation of these immune related genes in different tissues, which will contribute to better understand the pathogenesis and host defensive system in iridovirus invasion.

关键词： Megalocytivirus Red sea bream iridovirus (RSIV) Mandarin fish Histopathological analysis Immune related genes

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Exploration of low-carbon approximate probability design method for concrete structures

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CHINESE SCIENCE BULLETIN-CHINESE 2024年第27期69卷 4137-4150页

作者： Xiao, Jianzhuang Guan, Xiangshuo Xia, bing Ding, Tao Wang, Yuanfeng Xiao, xuwen Tongji Univ Coll Civil Engn Shanghai 200092 Peoples R China Guangxi Univ Coll Civil & Architecture Engn Nanning 530004 Peoples R China Tongji Univ Green Construct Res Ctr Shanghai 200092 Peoples R China Guangxi Univ Inst Sci & Technol Carbon Peak & Netrality Nanning 530004 Peoples R China Beijing Jiaotong Univ Carbin Neutralizat Tehnol & Strategy Res Ctr Beijing 100044 Peoples R China

With the world increasing concerns about climate change and environmental protection, reducing carbon emissions has become a crucial issue for various industries. With industrial development and rapid urbanization leading to a surge in energy demand, Chinas building-related carbon emissions have escalated in recent years. By 2020, the carbon emissions from construction industry accounted for a significant 50.9% of the total emissions nationwide. Concrete structures account for more than 60% of the total building stock in China, calling for the adoption of low-carbon design methods in concrete structures. Drawing upon the safety design process, this paper introduces a practical low-carbon probabilistic design approach. This method aims to facilitate engineering decision-making that meets the carbon reduction targets quantitatively, thus fostering sustainable development within the construction industry. By exploring the randomness inherent in carbon emissions, a low-carbon limit state equation was developed. To assess the level of concrete structures to meet carbon reduction targets, the concept of low-carbon index was introduced. This paper also distinguished between permanent carbon and variable carbon based on their sources. The former includes carbon emission from structural materials at the materialization stage and the end-of-life stage, while the latter encompasses operational carbon and embodied carbon from decorative materials and maintenance materials. Within these boundaries, two distinct low-carbon design paradigms were clarified: One focusing on structural embodied carbon and the other addressing whole life carbon in buildings. Considering the uncertainty of carbon emissions, the partial factor was introduced to simplify the probabilistic design into approximate probability design criteria. The carbon limits of concrete structures were deduced by a case study. Using Monte Carlo simulations, Chinas future building stock was predicted based on probabilisti

关键词： concrete structures low-carbon design approximate probability design carbon emission partial factor carbon limit

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Gut microbiota analysis of juvenile genetically improved farmed tilapia (Oreochromis niloticus) by dietary supplementation of different resveratrol concentrations

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FISH & SHELLFISH IMMUNOLOGY 2018年 77卷 200-207页

作者： Zheng, Yao Wu, Wei Hu, Gengdong Qiu, Liping Meng, Shunlong Song, Chao Fan, Limin Zhao, Zhixiang bing, xuwen Chen, Jiazhang Chinese Acad Fishery Sci Freshwater Fisheries Res Ctr 9 Shanshui East Rd Wuxi 214081 Jiangsu Peoples R China Minist Agr Fishery Ecoevironm Monitoring Ctr Lower Reaches Y Wuxi 214081 Jiangsu Peoples R China Minist Agr Lab Qual & Safety Risk Assessment Aquat Prod Envi Wuxi 214081 Jiangsu Peoples R China Nanjing Agr Univ Wuxi Fishery Coll Wuxi 214081 Jiangsu Peoples R China Minist Agr Key Lab Control Qual & Safety Aquat Prod Wuxi Jiangsu Peoples R China

The genetically improved farmed tilapia (GIFT, Oreochromis niloticus) is cultured widely for production of freshwater fish in China, while streptococcosis, likely related to pathogenic infections, occurs frequently in juvenile, mother, and operated GIFT. The gut microbiota plays an important role in nutrient digestibility in animals, and resveratrol (RES) has been used in feed for different freshwater fish species. Therefore, understanding changes in the tilapia gut microbiota across different concentrations of dietary RES supplementation is extremely important. The gut microbiota population in tilapia at 45 d after supplementation with different concentrations (0, 0.025, 0.05, 0.1 g/kg) of dietary RES was assessed by 16S rDNA gene sequencing. A total of 5445 operational taxonomic units were identified from all samples, and 14 phyla and 81 families were identified from all fecal samples. The bacteria of the phylum Firmicutes were significantly enriched in the 0.025 g/kg RES group when compared with the controls. Proteobacteria, Firmicutes and Cyanobacteria were the most dominant three phyla in all samples. With the increasing concentrations, the proportion of beneficial microbial taxa (Acetobacteraceae and Methylobacteriaceae) increased, whereas the proportion of harmful microbial taxa decreased, eg. Streptococcaceae except for 0.1 g/kg RES groups. RES did not affect the richness and diversity in tilapia gut microbiota. These findings provide information on the diversity and differences in GIFT gut microbiota database, and may contribute to developing strategies for management of diseases and long-term sustainability of O. niloticus culture.

关键词： Resveratrol GIFT Gut microbiota 16S rDNA Disease defense

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Production and Water Quality Responses in Tilapia Aquaculture Tanks Receiving Organic Fertilizers and Algae

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Agricultural Biotechnology 2019年第4期8卷 156-162页

作者： Shunlong MENG Dandan LI Liping QIU Gengdong HU Limin FAN Chao SONG Yao ZHENG Wei WU Jiazhang CHEN xuwen bing Freshwater Fisheries Research Center Chinese Academy of Fishery Sciences/Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze RiverMinistry of Agriculture/Key Laboratory of Fishery Eco-environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze RiverChinese Academy of Fishery SciencesWuxi 214081China Wuxi Fishery College Nanjing Agricultural UniversityWuxi 214081China

A two-month trial was carried out to evaluate the possibility of using algae and animal manure in tilapia *** the effect of algae,chicken manure and algae mixture together,cattle manure and algae mixture together,chicken-cattle manure and algae mixture together on the water quality and tilapia production was *** results showed that the yield of tilapia were 3.46,4.33,3.81,2.92 and 3.76 kg in Control,Algae,Chicken-A,Cattle-A and C-C-A,respectively,following the order of Algae>Chicken-A>C-C-A>Control>Cattle-A,and tilapia yield in Algae and chicken manure treatment groups increased by 25.0%and 10.1%respectively compared with the *** and chicken manure could increase the tilapia production,but cattle manure has no the *** the effect of algae and animal manure on water quality showed that adding chicken manure into tilapia pond could make water quality decreased at the beginning 20 d,but could increase water quality after 20 d and it can stabilize the phytoplankton structure in aquaculture *** Chlorella vulgaris and Scenedesmus quadricauda into tilapia pond could make water quality in a good state during the aquaculture process and it can stabilize the phytoplankton structure in aquaculture water.

关键词： Algae Chicken manure Cattle manure Tilapia production Water quality

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Effect of Organic Fertilizer on Aquaculture Water Quality and Tilapia Production

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Agricultural Biotechnology 2019年第5期8卷 92-98页

作者： Shunlong MENG Xi Chen Dandan LI Liping QIU Gengdong HU Limin FAN Chao SONG Yao ZHENG Wei WU Jiazhang CHEN xuwen bing Freshwater Fisheries Research Center Chinese Academy of Fishery Sciences/Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze RiverMinistry of Agriculture/Key Laboratory of Fishery Eco-environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze RiverChinese Academy of Fishery SciencesWuxi 214081China Wuxi Fishery College Nanjing Agricultural UniversityWuxi 214081China

A two-month trial was carried out in China to evaluate the possibility of recycling animal manure through pond tilapia *** the effects of chicken manure,cattle manure and chicken-cattle mixture together on the water quality and tilapia production were *** results showed that the yield of tilapia was 3.46,3.89,2.49 and 3.20 kg in the control,chicken M,cattle M,and chicken-cattle M,respectively,and the tilapia yields following the order of chicken M>control>chicken-cattle M>cattle *** tilapia yield in chicken M group increased by 12.43% compared with the *** manure could increase the tilapia production,but cattle manure has no the *** the effect of animal manure on water quality showed that adding chicken manure into tilapia pond could make water quality decreased at the beginning 20 d,but could increase water quality after 20 d and stabilize the phytoplankton structure.

关键词： Chicken manure Cattle manure Tilapia production Water quality Aquaculture

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基于Ecopath评估蟹-稻复合生态系统营养结构和能量流动

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生态学报 2020年第14期40卷 4852-4862页

作者：汪倩胡庚东宋超李丹丹郑尧裘丽萍范立明孟顺龙陈家长邴旭文中国水产科学研究院淡水渔业研究中心无锡214081 中国水产科学研究院内陆渔业生态环境和资源重点开放实验室无锡214081 农业农村部长江下游渔业生态环境监测中心无锡214081 南京农业大学无锡渔业学院无锡214081

蟹-稻综合种养模式为我国水产养殖以及农业从单一经营转变为复合经营提供了一种新模式,为了促进蟹-稻共作模式的推广和普及,应用Ecopath生态通道模型对该系统进行了营养结构和能量流动的分析。研究结果表明蟹-稻共作生态系统的最高营养... 详细信息

蟹-稻综合种养模式为我国水产养殖以及农业从单一经营转变为复合经营提供了一种新模式,为了促进蟹-稻共作模式的推广和普及,应用Ecopath生态通道模型对该系统进行了营养结构和能量流动的分析。研究结果表明蟹-稻共作生态系统的最高营养级(3.140)高于稻田单作系统的最高营养级(2.676),生态系统的能量流动主要有两条途径,即以沉水植物和其他维管束植物为起点的牧食链及以碎屑为起点的腐质链;蟹-稻共作农田生态系统在6月和10月的Finn′s循环指数(Finn′s cycling index,FCI,0.55和1)和Finn′s循环平均能流路径长度(Finn′s cycling mean path length,FCL,2.066和2.077),稻田单作农田生态系统在6月和10月的FCI(1.14和0.81)和FCL(2.089和2.137),说明蟹-稻共作模式可以形成一个稳定的生态系统,中华绒螯蟹的加入可以促进该完整的生态系统的能量流动,且随着螃蟹的成长,系统成熟度有所提高,只是较单作系统的成熟度低,共作系统有更大的成熟空间;蟹-稻共作农田生态系统在6月和10月的连接指数(Connectance index,CI)分别为0.135和0.135,稻田单作系统在6月和10月的CI为0.188和0.161,共作系统的各功能组之间的连接强度较单作系统弱,稳定程度相对较低,蟹-稻共作农田生态系统的初级生产力显著高于单作农田生态系统,且蟹-稻共作农田生态系统的发育良好。

关键词：生态通道模型蟹-稻复合生态系统营养结构能量流动

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彭泽鲫生长性状的遗传参数估计

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中国农学通报 2020年第33期36卷 134-137页

作者：徐金根曹烈金武何义进邴旭文王建民九江市水产科学研究所江西九江332000 中国水产科学研究院淡水渔业研究中心江苏无锡214081

为深入了解彭泽鲫(Carassius auratus ***)生长性状的遗传变化规律,并以此为基础制定彭泽鲫合理的育种方案。以九江市水产研究所培育的彭泽鲫23个全同胞家系的个体为实验材料,测量这些家系中1冬龄个体的生长性状(总长、体长、体高、体... 详细信息

为深入了解彭泽鲫(Carassius auratus ***)生长性状的遗传变化规律,并以此为基础制定彭泽鲫合理的育种方案。以九江市水产研究所培育的彭泽鲫23个全同胞家系的个体为实验材料,测量这些家系中1冬龄个体的生长性状(总长、体长、体高、体厚、体重),并用动物模型对数据进行遗传分析。结果表明:彭泽鲫1冬龄总长、体长、体高、体厚、体重5个性状的遗传力分别为0.64±0.17、0.51±0.16、0.79±0.18、0.57±0.16、0.65±0.17,均属于高水平遗传力。这5个性状之间表型相关(0.79~0.94)和遗传相关(0.95~1.00)均较高。采用个体选育或者个体选育与家系选择法相结合的方法对彭泽鲫进行遗传改良都能获得较好的遗传进展,且对某一生长性状进行选育时,其他性状也会得到间接改良。

关键词：彭泽鲫生长性状遗传力遗传参数家系选择

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Non-O1 Vibrio cholerae pathogen from Cyprinus carpio and control with anti-non-O1 V. cholerae egg yolk powder (IgY)

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AQUACULTURE 2017年 479卷 69-74页

作者： Gao, Xiaojian Chen, Nan Zhang, Yue Zhang, Xiaojun bing, xuwen Yangzhou Univ Coll Anim Sci & Technol Yangzhou Jiangsu Peoples R China Chinese Acad Fishery Sci Minist Agr Freshwater Fisheries Res Ctr Key Lab Freshwater Fisheries & Germplasm Utilizat Wuxi Peoples R China

Mass mortality in cultured common carp from a farm of Ganyu county, Jiangsu province, P. R. China was reported. The two dominated isolates named L1 and L2 from diseased common carps were confirmed as non-O1 Vibrio cholerae by biochemical characteristics and molecular tools. Virulence genes (ompW, hlyA, acfA, toxR, ace and rtxC genes) were found to be present in the isolates by PCR, which further confirmed pathogenicity of the isolates. The LD50 value of the isolate L1 to the common carp was 1.1 x 10(6) CFU/mL. In addition, the specific egg yolk powder (IgY) conferred significant protection of common carp against non-O1 V. cholerae. The specific IgY also exhibited significant bacteriolytic effects on the non-O1 V. cholerae pathogen. The results showed that the specific IgY should be an effective implemented feed additive for the biocontrol of non-O1 V. cholerae infection in aquaculture.

关键词： Cyprinus carpio Non-O1 Vibrio cholerae Virulence gene Egg yolk powder (IgY)

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Engineered Bacterial Outer Membrane Vesicles-Based Doxorubicin and CD47-siRNA Co-Delivery Nanoplatform Overcomes Immune Resistance to Potentiate the Immunotherapy of Glioblastoma

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ADVANCED MATERIALS 2025年第15期37卷 e2418053页

作者： You, Haoyu Zhang, Shilin Zhang, Yiwen Chen, Qinjun Wu, Yuxing Zhou, Zheng Zhao, Zhenhao Su, Boyu Li, xuwen Guo, Yun Chen, Yun Tang, Weiyi Liu, bing Fan, Hongrui Geng, Shuo Fang, Mingzhu Li, Fangxin Liu, Guangna Jiang, Chen Sun, Tao Fudan Univ Sch Pharm State Key Lab Med Neurobiol Minhang HospInnovat Ctr New Drug Dev Immune Infla Shanghai 201203 Peoples R China Fudan Univ MOE Frontiers Ctr Brain Sci Sch Pharm Dept Pharmaceut Shanghai 201203 Peoples R China Fujian Med Univ Affiliated Hosp 1 Natl Reg Med Ctr Dept Orthoped Binhai Campus Fuzhou 350212 Peoples R China Quzhou Fudan Inst Quzhou 324003 Peoples R China

Apart from the blood-brain barrier (BBB), the efficacy of immunotherapy for glioblastoma (GBM) is limited by the presence of intrinsic and adaptive immune resistance, implying that co-delivery of various immunotherapeutic agents or simultaneous regulation of different cells is urgently needed. Bacterial outer membrane vesicles (OMVs) offer a unique advantage in the treatment of GBM, owing to their multifunctional properties as carriers and immune adjuvants and their ability to cross the BBB. However, traditional OMVs can lead to toxic side effects and disruption of tight junctions in the BBB. Therefore, to enhance the in vivo safety and targeting capability of OMVs, we introduced engineered OMVs to reduce toxicity and further constructed a modularly assembled nanoplatform by performing simple peptide modifications. This nanoplatform demonstrates satisfactory biosafety and is able to continuously cross the BBB and target GBM with the assistance of Angiopep-2. Subsequently, immunogenic substances on OMVs, along with carried small-interfering RNA (siRNA) and doxorubicin, can promote and enhance the reprogramming and phagocytic abilities of macrophages and microglia, respectively, and increase the immunogenicity of GBM, ultimately overcoming GBM immune resistance to enhance the efficacy of immunotherapy. This OMVs-based nanoplatform provides a new paradigm and insights into the development of immunotherapy for GBM.

关键词： engineering technology glioblastoma immune resistance immunotherapy outer membrane vesicles

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