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上海海洋大学学报 2018年第1期27卷 98-105页

作者：郑尧陈家长胡庚东赵志祥孟顺龙范立民宋超邴旭文中国水产科学研究院淡水渔业研究中心江苏无锡214081 农业部水产品质量安全环境因子风险评估实验室江苏无锡214081 南京农业大学无锡渔业学院江苏无锡214081

通过测定TOC、COD、Chl、TN、NH_4^+-N、NO_2^--N、NO_3^--N、TP、PO_4^(3-)-P等水质指标和底泥中TOC、TN、TP指标,探究"空心菜-水芹"轮作模式对不同养殖品种和养殖数量情况下养殖池塘水质和底质环境的影响。结果表明,在轮作... 详细信息

通过测定TOC、COD、Chl、TN、NH_4^+-N、NO_2^--N、NO_3^--N、TP、PO_4^(3-)-P等水质指标和底泥中TOC、TN、TP指标,探究"空心菜-水芹"轮作模式对不同养殖品种和养殖数量情况下养殖池塘水质和底质环境的影响。结果表明,在轮作模式前期,空心菜(Ipomoea aquatica)种植能显著降低甘露青鱼(Mylopharyngodon piceus)养殖场TOC、Chl、TN、NH_4^+-N、NO_2^--N、NO_3^--N、PO_4^(3-)-P等水质指标,能显著降低苏州经济鱼亲本塘TOC、COD、Chl、NH_4^+-N、NO_2^--N、NO_3^--N、TP等水质指标。轮作后期水芹(Oenanthe stolonifera)种植能降低甘露青鱼养殖场TOC、NO_3^--N、TP等水质指标,降低苏州经济鱼亲本塘TOC、COD、NH_4^+-N、NO_3^--N、TP等水质指标。轮作前、后期均能降低底质TOC、TN和TP含量。"空心菜-水芹"轮作模式能显著降低养殖池塘水体中TOC、NH_4^+-N、NO_3^--N、TP指标和底泥中TOC、TN、TP指标。

关键词：水质底泥轮作原位修复

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南疆中熟陆地棉化学打顶与后期管理技术

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中国棉花 2022年第5期49卷 44-45页

作者：王刚陈兵王旭文马晓梅新疆农垦科学院新疆石河子832000

为满足南疆棉花生产全程机械化和节本高效的要求,根据多年小区试验与大田生产实践,结合南疆地区气候、土壤、品种等因素,提出了南疆中熟陆地棉化学打顶与后期管理技术,包括:适用范围、药剂选择、喷药要求、施药技术与注意事项、后期管... 详细信息

为满足南疆棉花生产全程机械化和节本高效的要求,根据多年小区试验与大田生产实践,结合南疆地区气候、土壤、品种等因素,提出了南疆中熟陆地棉化学打顶与后期管理技术,包括:适用范围、药剂选择、喷药要求、施药技术与注意事项、后期管理技术等,以期为该技术在南疆大面积示范推广提供参考。

关键词：南疆陆地棉化学打顶后期管理

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新疆棉花生产全程机械化机艺融合技术

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中国棉花 2022年第3期49卷 24-26页

作者：王刚陈兵王旭文马晓梅新疆农垦科学院新疆石河子832000

针对新疆棉花耕地连作障碍突出、肥水管理粗放、全程机械化比例偏低等因素导致的棉花生产成本高、棉农植棉效益下降等问题,分析了实现棉花生产机械化和轻简化转型升级的途径,总结了新疆棉花全程机械化机艺融合技术,包括:选地整地、精量... 详细信息

针对新疆棉花耕地连作障碍突出、肥水管理粗放、全程机械化比例偏低等因素导致的棉花生产成本高、棉农植棉效益下降等问题,分析了实现棉花生产机械化和轻简化转型升级的途径,总结了新疆棉花全程机械化机艺融合技术,包括:选地整地、精量精准播种、种植模式、深松、水肥药一体化、化学调控、无人机飞防、化学脱叶、机械采收、秸秆还田与残膜回收等各项技术,以期为新疆棉花可持续发展提供技术先导和引领示范。

关键词：棉花机械化机艺融合新疆

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虎杖浮床净水能力及根叶中虎杖苷含量测定

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中国农学通报 2018年第36期34卷 88-92页

作者：郑尧赵志祥史磊磊邴旭文陈家长中国水产科学研究院淡水渔业研究中心/农业部长江下游渔业生态环境监测中心/农业部水产品质量安全环境因子风险评估实验室(无锡) 江苏无锡214081 南京农业大学无锡渔业学院江苏无锡214081

通过构建虎杖浮床对其净水能力进行研究,高效液相测定土生、水生虎杖根、叶中虎杖苷含量。结果显示:水生虎杖生长状况良好,粗壮浮床根越冬后第二年能正常发芽,在一定时间段内可持续使用;3.5%(虎杖重/水体重)虎杖20天对养殖水体中TN、TP... 详细信息

通过构建虎杖浮床对其净水能力进行研究,高效液相测定土生、水生虎杖根、叶中虎杖苷含量。结果显示:水生虎杖生长状况良好,粗壮浮床根越冬后第二年能正常发芽,在一定时间段内可持续使用;3.5%(虎杖重/水体重)虎杖20天对养殖水体中TN、TP、NO_3--N、NH4+-N去除率分别达到69.4%、71.3%、90.3%、74.1%,虎杖对污水各污染指标中NO_3--N的吸收能力最强,其次是TN、TP、NH4+-N、NO_2--N。土生虎杖根虎杖苷含量约为水中根的10倍,土生叶中虎杖苷含量比水生叶高出约50%。

关键词：虎杖浮床虎杖苷净水能力

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Molecular Cloning and Characteristics Analysis of ghrelin Gene in Asian Swamp Eel(Monopterus albus)

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Agricultural Science & Technology 2016年第4期17卷 769-774页

作者： Guoliang RUAN Kai LIAO Daiqin YANG xuwen bing Engineering Research Center of Ecology and Agricultural Use of Wetland Ministry of Education/College of Animal ScienceYangtze UniversityJingzhou 434025China Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilizations Ministry of Agriculture/Freshwater FisheriesResearch CenterChinese Academy of Fisheries SciencesWuxi 214081China Hubei Collaborative Innovation Center for Freshwater A uaculture Wuhan 430070China

Ghrelin is an important signaling molecule linking reproductive and energy metabolism. In this study, ghrelin gene of Monopterus albus was cloned. Its structure and function were analysized preliminarily. By Rapid Amplification of cDNA Ends(RACE) technique, full-length cDNA and DNA sequences of ghrelin gene were obtained. The full-length ghrelin cDNA(GenBank accession no. JX122807) was 552 bp long, containing a 115 bp 5'-untranslated region, a 324 bp open reading frame and a 113 bp 3'-untranslated region. The full-length ghrelin DNA was 1 323 bp, consisting of three introns and four exons. The exon/intron junction sequences conformed to the GT/AG rule. Three introns were 594, 84 and 93 bp in length, respectively; four exons were229, 78, 112 and 133 bp in length, respectively. The results of amino acid sequence analysis showed that the deduced propreghrelin sequence of M. albus contained a signal peptide(SP) consisting of 22 amino acid residues, a mature peptide(MP)consisting of 19 amino acid residues and a C-terminal amino acid residue. Among them, the third amino acid of MP was serine(Ser^3) as the site for N-acylation and N-deacetylation reactions; the C-terminal amino acid residue sequence might contain a peptide hormone obestatin, which is a physiological antagonist of mature Ghrelin peptide. The homology and phylogenic relationships analyses of amino acid sequences suggested that propreghrelin of M. albus had high similarity to those of several Perciformes fishes; the propreghrelins of M. albus, Perciformes and Heterosomata fishes were clustered into a subgroup. The high conservatism of the gene structure and the amino acid sequences indicated that Ghrelin exerts important physiological functions and plays similar physiological mechanisms in vertebrates.

关键词： Monopterus albus ghrelin gene Clone Molecular structure

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不同化学打顶剂对新疆棉花群体质量的影响

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中国棉花 2021年第10期48卷 21-24页

作者：王刚韩焕勇王旭文陈兵新疆农垦科学院新疆石河子832000

2020年以新陆早74号为研究对象,以人工打顶为对照,研究了甲哌钅翁和氟节胺不同剂量处理对棉花群体质量指标的影响,为棉花化学打顶技术的推广提供科学依据。结果表明:使用2种化学打顶剂后棉花叶面积指数、群体光吸收率、群体光合速率都... 详细信息

2020年以新陆早74号为研究对象,以人工打顶为对照,研究了甲哌钅翁和氟节胺不同剂量处理对棉花群体质量指标的影响,为棉花化学打顶技术的推广提供科学依据。结果表明:使用2种化学打顶剂后棉花叶面积指数、群体光吸收率、群体光合速率都随剂量的增加而下降,中剂量处理干物质积累量和蕾铃分配比最佳,同时中剂量处理的株高、单株果枝数、皮棉产量均高于对照处理。本研究初步明确了2种化学打顶剂不同剂量处理对棉花群体质量指标的影响,并证明了化学打顶替代人工打顶的可行性。

关键词：化学打顶剂群体质量棉花新疆叶面积指数光吸收率光合速率干物质积累量

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基于Sentinel-2与随机森林算法的太湖水生植被分布监测

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环境监控与预警 2023年第6期15卷 42-49页

作者：张悦陈冰李旭文牛志春姜晟李继影崔嘉宇余悠然江苏省环境监测中心江苏南京210019 江苏省生态环境大数据有限公司江苏南京210019 南京信息工程大学江苏南京210044 江苏省苏州环境监测中心江苏苏州215004

基于2018—2021年太湖地区哨兵2号(Sentinel-2)卫星遥感影像和随机森林算法,结合植被敏感指数,开展太湖水生植被分布监测,综合分析了水生植被的时空变化特征,初步揭示了太湖水生植被的动态变化。结果表明,太湖水生植被分布面积有逐年上... 详细信息

基于2018—2021年太湖地区哨兵2号(Sentinel-2)卫星遥感影像和随机森林算法,结合植被敏感指数,开展太湖水生植被分布监测,综合分析了水生植被的时空变化特征,初步揭示了太湖水生植被的动态变化。结果表明,太湖水生植被分布面积有逐年上升的趋势,且年间变化具有明显的单峰型特征,表现为春季沉寂,夏季快速增长,秋季达到暴发期,冬季面积开始减退;太湖水生植被主要分布于东太湖、东部沿岸和贡湖,类型以沉水植被为主,其中浮叶植被主要分布于东太湖,挺水植被主要分布于东太湖沿岸的浅水区域;沉水植被主要分布于东部沿岸和贡湖;水生植被主要分布的东太湖水域,其水质总体优于太湖其他水域。

关键词：哨兵2号卫星随机森林模型水生植被水质太湖

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Mapping human tissues with highly multiplexed RNA in situ hybridization

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NATURE COMMUNICATIONS 2024年第1期15卷 2511页

作者： Kalhor, Kian Chen, Chien-Ju Lee, Ho Suk Cai, Matthew Nafisi, Mahsa Que, Richard Palmer, Carter R. Yuan, Yixu Zhang, Yida Li, xuwen Song, Jinghui Knoten, Amanda Lake, Blue B. Gaut, Joseph P. Keene, C. Dirk Lein, Ed Kharchenko, Peter V. Chun, Jerold Jain, Sanjay Fan, Jian-bing Zhang, Kun Univ Calif San Diego Dept Bioengn La Jolla CA 92093 USA Univ Calif San Diego Program Bioinformat & Syst Biol La Jolla CA 92093 USA Univ Calif San Diego Dept Elect Engn La Jolla CA USA Sanford Burnham Prebys Med Discovery Inst La Jolla CA USA Univ Calif San Diego Program Biomed Sci La Jolla CA USA Harvard Med Sch Dept Biomed Informat Boston MA USA Altos Labs San Diego CA 94022 USA Washington Univ Sch Med Dept Med St Louis MO USA Washington Univ Sch Med Dept Pathol & Immunol St Louis MO USA Univ Washington Dept Lab Med & Pathol Sch Med Seattle WA USA Allen Inst Brain Sci Seattle WA 98103 USA Illumina San Diego CA USA

In situ transcriptomic techniques promise a holistic view of tissue organization and cell-cell interactions. There has been a surge of multiplexed RNA in situ mapping techniques but their application to human tissues has been limited due to their large size, general lower tissue quality and high autofluorescence. Here we report DART-FISH, a padlock probe-based technology capable of profiling hundreds to thousands of genes in centimeter-sized human tissue sections. We introduce an omni-cell type cytoplasmic stain that substantially improves the segmentation of cell bodies. Our enzyme-free isothermal decoding procedure allows us to image 121 genes in large sections from the human neocortex in <10 h. We successfully recapitulated the cytoarchitecture of 20 neuronal and non-neuronal subclasses. We further performed in situ mapping of 300 genes on a diseased human kidney, profiled >20 healthy and pathological cell states, and identified diseased niches enriched in transcriptionally altered epithelial cells and myofibroblasts.

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Molecular mechanism of endocrine system impairment by 17α-methyltestosterone in gynogenic Pengze crucian carp offspring

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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2016年 128卷 143-152页

作者： Zheng, Yao Chen, Jiazhang Liu, Yan Gao, Jiancao Yang, Yanping Zhang, Yingying bing, xuwen Gao, Zexia Liang, Hongwei Wang, Zaizhao Northwest A&F Univ Coll Anim Sci & Technol Shaanxi Key Lab Mol Biol Agr Yangling 712100 Shaanxi Peoples R China Chinese Acad Fishery Sci Key Open Lab Ecol Environm & Resources Inland Fis Freshwater Fisheries Res Ctr Beijing Peoples R China Minist Agr Key Lab Genet Breeding & Aquaculture Biol Freshwa Sci Observing & Expt Stn Fishery Resources & Envi Beijing Peoples R China Nanjing Agr Univ Wuxi Fisheries Coll Wuxi 214081 Peoples R China HZAU Minist Agr Key Lab Freshwater Anim Breeding Wuhan 430070 Peoples R China Chinese Acad Fishery Sci Yangtze River Fisheries Res Inst Wuhan 430223 Hubei Peoples R China

The effects of synthetic androgen 17 alpha-methyltestosterone (MT) on endocrine impairment were examined in crucian carp. Immature 7-month old mono-female Pengze crucian carp (Pcc) F2 offspring were exposed to 50 and 100 mu g/L of MT (week 2, 4, and 8). Gonadosomatic index, hepatosomatic index and intestine weight altered considerably and oocyte development was repressed. In the treatment groups, ovarian 11-ketotestosterone decreased, whereas 17 beta-estradiol and testosterone increased, and ovarian aromatase activities increased at week 4. However, in the brain tissue, those values, significantly decreased. Quantitative RT-PCR analysis demonstrated changes in steroid receptor genes and upregulation of steroidogenic genes (Pcc-3bhsd, Pcc-11bhsd2 Pcc-cyp11 alpha 1), while the other three steroidogenic genes (Pcc-cyp17 alpha 1, Pcc-cyp19a1a and Pcc-star) decreased from week 4 to week 8. Ovarian, hepatic Pcc-vtg B and vitellogenin concentration increased in both 50 and 100 a of MT exposure groups. This study adds further information regarding the effects of androgens on the development of previtellogenic oocytes, which suggests that MT could directly target estrogen signaling pathway, or indirectly affect steroidogenesis and vitellogenesis. (C) 2016 Elsevier Inc. All rights reserved.

关键词： 17 alpha-methyltestosterone Estrogen signalling Gynogenic Pengze crucian carp Steroid hormones Steroidogenesis Vitellogenesis

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DNA Facilitates the Sorption of Polycyclic Aromatic Hydrocarbons on Montmorillonites

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ENVIRONMENTAL SCIENCE & TECHNOLOGY 2018年第5期52卷 2694-2703页

作者： Qin, Chao Zhang, Wei Yang, bing Chen, xuwen Xia, Kang Gao, Yanzheng Nanjing Agr Univ Coll Resource & Environm Sci Inst Organ Contaminant Control & Soil Remediat Nanjing 21009S Jiangsu Peoples R China Michigan State Univ Dept Plant Soil & Microbial Sci E Lansing MI 48824 USA Michigan State Univ Environm Sci & Policy Program E Lansing MI 48824 USA Virginia Tech Dept Crop & Soil Environm Sci Blacksburg VA 24060 USA

The sorption of polycyclic aromatic hydrocarbons (PAHs) to montmorillonites is largely influenced by their interactions with dissolved organic matter (DOM). However, the role of DOM rather than humic and fulvic acids (e.g., extracellular DNA) in the PAH sorption to soil clays is little known. Here, we demonstrate that extracellular double-stranded salmon testes DNA substantially increased the sorption of phenanthrene and pyrene to Na-, Ca-, and Fe-modified montmorillonites. All PAH sorption isotherms fitted the linear and Freundlich models reasonably well (R-2 = 0.918-0.999). Distribution coefficients were increased from 0.0458-0.103 and 0.0493-0.141 L/g at 0 mg/L DNA to 0.413-0.589 and 0.385-0.560 L/g at 10 mg/L DNA for phenanthrene and pyrene, respectively. Spectroscopic and computational chemistry analyses confirmed that PAHs were first inserted into DNA by binding with the nudeobases via van der Waals and pi-pi electron donor-acceptor interactions. Compared to PAHs, the DNA-PAH complex can be more easily sorbed to cation-modified montmorillonites by complexation between DNA phosphate and exchangeable cations in addition to intercalation into clay interlayers. This work highlights the importance of understanding the control on contaminant sorption by many organic compounds that are ubiquitous in soils but not represented by humic and fulvic acids.

关键词： AROMATIC compounds ORGANIC compounds in water FULVIC acids SORPTION DNA HUMIC acid

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