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Hemoglobin assisted one-pot synthesis of MnO2 nanozyme for radiation protection

JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY 2022年 77卷

作者： Wang, Cheng Liu, Li Chen, Shaoqing cui, Pengfei Zhou, shuwen Qiu, Lin Jiang, Pengju Wang, Jianhao Ni, Xinye Nanjing Med Univ Affiliated Changzhou Peoples Hosp 2 Changzhou Jiangsu Peoples R China Changzhou Univ Sch Pharm Changzhou Jiangsu Peoples R China Jiangsu Prov Engn Res Ctr Med Phys Changzhou 213003 Jiangsu Peoples R China

The ongoing development of nuclear technologies, especially in medical diagnosis, calls for attention to radiation protection. Herein, a MnO2 nanozyme with peroxidase activity was synthesized using hemoglobin (HGB) as the reducing agent and with a facile one-method method. The HGB-MnO2 nanozyme showed good dispersity and near spherical morphology with size of about 5 nm. In addition, the HGB-MnO2 nanozyme showed high colloidal stability and decent scavenging ability on different radicals (DPPH, ABST, hydroxyl, and superoxide radicals). Therefore, the HGB-MnO2 nanozyme can effectively lower the intracellular ROS level, reduce the DNA damage and maintain the cell viability of HUVECs under X-ray irradiation. The in vivo experiments using C57BL/6J mice as the model also revealed a longer survival time in HGB-MnO2 nanozyme treated group under X-ray radiation without causing observable damage to the subjects. Therefore, HGB-MnO2 nanozyme can be considered as an effective and safe formulation for radiation protection.

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Slower than expected reduction in annual PM2.5 in Xi'an revealed by machine learning-based meteorological normalization

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SCIENCE OF THE TOTAL ENVIRONMENT 2022年 841卷 156740页

作者： Wang, Meng Zhang, Zhuozhi Yuan, Qi Li, Xinwei Han, shuwen Lam, Yuethang cui, Long Huang, Yu Cao, Junji Lee, Shun-cheng Hong Kong Polytech Univ Dept Civil & Environm Engn Hong Kong Peoples R China Chinese Acad Sci Inst Earth Environm State Key Lab Loess & Quaternary Geol Xian 710061 Peoples R China Chinese Acad Sci Inst Atmospher Phys Key Lab Middle Atmosphere & Global Environm Obser Beijing 100029 Peoples R China

To evaluate the effectiveness of air pollution control policies, trend analysis of the air pollutants is often performed. However, trend analysis of air pollutants over multiple years is complicated by the fact that changes in meteorology over time can also affect the levels of air pollutants in addition to changes in emissions or atmospheric chemistry. To decouple the meteorological effect, this study performed a trend analysis of the hourly fine particulate matter (PM2.5) observed at an urban background site in Xi'an city over 5 years from 2015 to 2019 using the machine learning algorithm. As a novel way of meteorological normalization, the meteorological parameters were used as constant input for 5 consecutive years. In this way, the impact of meteorological parameters was excluded, providing insights into the real changes in PM2.5 due to changes in emission strength or atmospheric chemistry. After meteorological normalization, a decreasing trend of -3.3 % year(-1) (-1.9 mu g m(-3) year(-1)) in PM2.5 was seen, instead of -4.4 % year(-1) from direct PM2.5 observation. Assuming the rate of -1.9 mu g m(-3) year(-1) were kept constant for the next few decades in Xi'an, it would take approximately 25 years (in the year 2045) to reduce the annual PM2.5 level to 5 mu g m(-3), the new guideline value from World Health Organization. We also show that PM2.5 is primarily associated with anthropogenic emissions, which, underwent aqueous phase chemistry in winter and photochemical oxidation in summer as suggested by partial dependence of RH and O-x in different seasons. Therefore, reducing the anthropogenic secondary aerosol precursors at a higher rate, such as NOx and VOCs is expected to reduce the particulate pollution in this region more effectively than the current -3.3 % year(-1) found in this study.

关键词： Particulate matter Secondary aerosol Theil-Sen estimator Random forest Aqueous phase chemistry

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马立克病病毒MDLV21株对SPF鸡的致病性与免疫保护

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中国动物传染病学报 2025年第1期33卷 44-49页

作者：安子齐赵亚楠王淑雯徐敏丽崔宁许传田苏帅山东农业大学动物医学院山东省动物生物工程与疾病防治重点实验室泰安271018 山东省农业科学院畜牧兽医研究所山东省畜禽疫病防治与繁育重点实验室济南250100

为了研究缺失RLORF6、Meq基因的鸡马立克病病毒(MDV)MDLV21株对SPF鸡的致病性与免疫保护效果。通过接种SPF鸡,比较MDLV21株对鸡的体重、免疫器官指数、灭活疫苗诱导抗体水平的影响并统计死亡率和肿瘤率;通过攻毒保护试验,统计分析MDLV2... 详细信息

为了研究缺失RLORF6、Meq基因的鸡马立克病病毒(MDV)MDLV21株对SPF鸡的致病性与免疫保护效果。通过接种SPF鸡,比较MDLV21株对鸡的体重、免疫器官指数、灭活疫苗诱导抗体水平的影响并统计死亡率和肿瘤率;通过攻毒保护试验,统计分析MDLV21株与CVI988/Rispens株对MDV超强毒株Md5的免疫保护指数。结果显示:MDLV21株接种SPF鸡的体重、胸腺和法氏囊免疫器官指数均与对照组SPF鸡差异不显著;接种鸡与对照组鸡的禽流感、新城疫抗体滴度相似。MDLV21株接种鸡在整个周期没有死亡以及肿瘤发生,脾脏、肝脏组织切片显示细胞结构正常。MDLV21株、CVI988/Rispens株对感染Md5的SPF鸡分别提供90%、70%的免疫保护。综上,MDV MDLV21株对SPF鸡没有致病性,对MDV Md5株具有良好的免疫保护。本研究可为MD的防控提供1株新的生物安全性、免疫效果良好的疫苗候选株。

关键词：马立克病毒 RLORF6基因 Meq基因缺失株致病性免疫保护效果

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Fluorescent whitening agents in Baiyangdian Lake in North China: Analysis, occurrence, distribution and ecological risk assessment

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ENVIRONMENTAL POLLUTION 2021年 291卷 118235页

作者： Wu, Qi He, Bowen Guo, Ruiyao cui, Jiansheng Yan, shuwen Liu, Daxi Yao, Bo Hebei Univ Sci & Technol Sch Environm Sci & Engn Shijiazhuang 050018 Hebei Peoples R China Univ Sci & Technol China Sch Rare Earths Hefei 230026 Peoples R China Chinese Acad Sci Ganjiang Innovat Acad Ganzhou 341000 Peoples R China Fudan Univ Dept Environm Engn Sci Shanghai 200433 Peoples R China

Fluorescent whitening agents (FWAs) are very important chemical additives that are widely applied in the industrial production field. The history of global FWA production and use spans more than 60 years, but the environmental fate of FWAs has been less reported in the public literature and most studies predate 2000;in addition, the studied FWAs were still limited to FWA71 and FWA351. In this study, the occurrence and distribution of 9 commonly used FWAs in a lake in North China were reported for the first time. We found that 6 target FWAs were prevalent in the lake, and the concentration levels were usually at the ng L-1 level. Decreasing FWA levels with increasing distance from the estuary area were observed in summer. FWA135, FWA185, and FWA367, the most detected 3 FWAs, with the ecological risk at high levels, and sigma RQ >10 were obtained from all the investigated samples, suggesting that all the sampling sites could be considered with certain ecological risk for aquatic life. As a category of heavily and widely used dyes, FWAs in environmental media have been ignored for a long time. Substantial additional research needs to be conducted to determine the environmental behavior and ecological toxicology of FWAs.

关键词： Optical brighteners Liquid chromatography-mass spectrometry Online solid phase extraction Risk quotients

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Glucose oxidase loaded thermosensitive hydrogel as an antibacterial wound dressing

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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY 2022年 76卷

作者： Fang, Xiuling Liu, Yadong Zhang, Miaomiao Zhou, shuwen cui, Pengfei Hu, Huaanzi Jiang, Pengju Wang, Cheng Qiu, Lin Wang, Jianhao Changzhou Univ Sch Pharm Changzhou 213164 Peoples R China

Skin infection is a worldwide issue that requires joint efforts to develop suitable wound dressing. Hydrogel as the ideal form of wound dressing usually suffered from complicated preparation, low reproducibility and high toxicity/cost. Therefore, the development of cost effective and biocompatible materials to conveniently construct hydrogel based wound dressing in a facile and reproducible way should be one of the future orientation in this field. Here in this study, we proposed a simple thermosensitive hydrogel composed of cost effective and biocompatible Pluronic F-68 and Pluronic F-127, entitled F68/F127 hydrogel. The F68/F127 hydrogel was further loaded with glucose oxidase (GOx@F68/F127 hydrogel) as an antibacterial wound dressing, which showed preferable sol-gel transformation property upon application to the wounds with powerful antibacterial effects. In this way, GOx@F68/F127 hydrogel showed promising inhibition of both Gram positive and negative strains in vitro and in vivo. The antibacterial rate of GOx@F68/F127 reached 100% for Staphylococcus aureus at 0.65 mu g/mL and 100% for Escherichia coli at 0.6 mu g/mL. And it has high biocompatibility and the ability to promote wound healing. At the end of treatment, mice in the GOx@F68/F127 group had 98% wound healing relative to the control group, which had 30% wound healing. Therefore, GOx@F68/F127 hydrogel can be applied as a promising antimicrobial wound excipient.

关键词： F68 F127 hydrogel GOx@F68 Antibacterial Wound healing

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张掖市山丹县马铃薯枯萎病菌的鉴定

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中国蔬菜 2024年第1期 68-73页

作者：何树文王彦崔凌霄许永锋王泽浩杨成德张掖市植保植检站甘肃张掖734000 甘肃农业大学植物保护学院甘肃兰州730070

为明确甘肃省马铃薯枯萎病菌种类,对采集自山丹县的马铃薯枯萎病病原菌进行分离和致病性测定,结合其形态学特征和分子生物学分析对病原菌进行分类鉴定。结果表明,分离得到的3株真菌分离物,经致病性测定均使马铃薯表现出枯萎病的典型症状... 详细信息

为明确甘肃省马铃薯枯萎病菌种类,对采集自山丹县的马铃薯枯萎病病原菌进行分离和致病性测定,结合其形态学特征和分子生物学分析对病原菌进行分类鉴定。结果表明,分离得到的3株真菌分离物,经致病性测定均使马铃薯表现出枯萎病的典型症状,其中病原菌3-2的大型分生孢子呈镰刀状,多为3~5个分隔,大小为(50~75)μm×(4~5)μm,小型分生孢子呈椭圆形;病原菌1-1菌落表面中心由白色逐渐变为红色,边缘白色,呈现不规则状,未观察到分生孢子;病原菌3-6菌丝红色,生长后期菌落中心表面呈黄色,菌丝有隔,厚垣孢子圆形,未观察到分生孢子。结合形态学特征、ITS与EF-1α基因序列同源性,将病原菌3-2鉴定为木贼镰刀菌(Fusarium equiseti),该病菌引起的马铃薯枯萎病为国内首次报道;将1-1和3-6鉴定为三线镰孢菌(F. tricinctum),该病菌引起的马铃薯枯萎病为甘肃省首次报道。

关键词：马铃薯枯萎病鉴定致病性

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Antibacterial photodynamic peptides for staphylococcal skin infection

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BIOMATERIALS SCIENCE 2020年第23期8卷 6695-6702页

作者： Lei, Xiaoling Qiu, Lin Lan, Min Du, Xuancheng Zhou, shuwen cui, Pengfei Zheng, Ronghui Jiang, Pengju Wang, Jianhao Xia, Jiang Changzhou Univ Sch Pharm Changzhou 213164 Jiangsu Peoples R China Shandong Univ Sch Phys Inst Adv Interdisciplinary Sci Jinan 250100 Peoples R China Changzhou Sun Pharmaceut Co Ltd Changzhou 213125 Jiangsu Peoples R China Chinese Univ Hong Kong Dept Chem Shatin Hong Kong Peoples R China

As the barrier between the human body and the outside world, the skin is vulnerable to pathogenic microorganisms, especially when suffering from skin injuries such as burns. Staphylococcus aureus remains the most common type of bacteria that infects humans, and the surging drug resistance poses a major threat to the treatment of these infections. Here we report the development of antibacterial photodynamic peptides (APPs) that are constructed based on the covalent conjugation of an antibacterial peptide and the photosensitizer chlorin e6 (Ce6). Peptide conjugation significantly increases the photo-stability of Ce6, while retaining its ROS generation capability under photo-irradiation. The APPs combine the antibacterial activity of the peptide and the photodynamic therapy of Ce6, and under the assistance of mild laser irradiation, can eradicate bacterial infection and inhibit the formation of bacterial biofilms ex vivo. One of the APPs, (GKRWWKWWRR)(2)KGGK(Ce6)G, AMP(2)-Ce6, with Ce6 conjugated with the dimeric peptide, showed exceptional antibacterial activity with an MIC90 value around 3.2 mu M without photo-irradiation and <0.1 mu M with short light treatment. Supported by a hydrogel matrix composed of gelatin and recombinant human collagen III protein (rhCol III) mimicking the extracellular matrix of skin cells, AMP(2)-Ce6 efficiently accelerated the healing rate of wounds and improved the quality of wound healing in mice infected with Staphylococcus aureus. Altogether, here we report the development of antibacterial photodynamic peptides, which together with a regenerative matrix material exhibit an added effect against staphylococcal skin infection. This composite material holds promise as a new type of wound dressing material for skin infection and wound healing.

关键词： Peptides

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High-performance microporous polymer membranes prepared by interfacial polymerization for gas separation

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JOURNAL OF MEMBRANE SCIENCE 2019年 573卷 425-438页

作者： Yu, shuwen Li, Shichun Liu, Yu cui, Sheng Shen, Xiaodong Nanjing Tech Univ Coll Mat Sci & Engn Nanjing 210009 Jiangsu Peoples R China China Acad Engn Phys Inst Chem Mat Mianyang 621900 Peoples R China Nanjing Tech Univ Jiangsu Collaborat Innovat Ctr Adv Inorgan Funct Nanjing 210009 Jiangsu Peoples R China Univ Cambridge Dept Mat Sci & Met Cambridge CB3 0FS England

Microporous polymers possessing good processability and low cost show promising applications for large-scale industrial membrane separation. The relatively low gas selectivity and physical aging of microporous polymer membranes are current challenges which become more serious for ultrathin membranes. Crosslinking is an effective way to improve gas selectivity and physical aging resistance of glassy polymers, and the cross-linked ultrathin membranes can be directly produced by interfacial polymerization (IP). However, there are only a few reports of IP formed microporous polymer membranes, and the reported gas separation performance is not satisfying. In this work, ultrathin microporous polyarylate (PAR) membranes were prepared by IP of 5,5', 6,6'-tetrahydroxy-3,3,3',3'-tetramethylspirobisindane (TTSBI) and trimesoyl chloride (TMC). The crosslinking structure, molar ratio of TTSBI to TMC (n((TTSBI))/n((TMC))) in PAR, and membrane thickness characterized by zeta potential measurements, X-ray photoelectron spectroscopy and scanning electronic microscope were optimized by adjusting IP parameters such as pH value of aqueous phase that decides the hydrolysis of both TTSBI and TMC in IP, and the concentrations of TTSBI and TMC. The microporous structures of PAR characterized by CO2 adsorption are highly relevant to the n((TTSBI))/n((TMC)) in PAR. The resulted membranes exhibit CO2 permeance in a wide range of 100-2115 GPU with CO2/N-2 selectivity of 45-21 that is a remarkable improvement in gas separation performance of IP formed microporous polymer membranes and also shows significant advantages over other reported ultrathin microporous polymer membranes. In addition, the membranes also exhibit excellent stability, and only 29% decrease in CO2 permeance was observed after 180 h aging. This work provides an effective strategy to fabricate high-performance and stable microporous polymer membranes by IP, which is expected to push the particle applications of microporous poly

关键词： Microporous polymer Ultrathin membrane Thin film composite (TFC) Gas separation Interfacial polymerization

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Multienzyme detection and in-situ monitoring of enzyme activity by bending CE using quantum dots-based polypeptide substrate

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ELECTROPHORESIS 2020年第12期41卷 1103-1108页

作者： Qiu, Lin cui, Pengfei Zhu, Zhilan Xu, Mingyu Jia, Wenjing Sheng, Jingyu Ni, Xinye Zhou, shuwen Wang, Jianhao Changzhou Univ Sch Pharmaceut Engn & Life Sci Changzhou Jiangsu Peoples R China Nanjing Med Univ Affiliated Changzhou 2 Peoples Hosp Changzhou 213003 Jiangsu Peoples R China Changzhou Le Sun Pharmaceut Co Ltd Changzhou Jiangsu Peoples R China Jiangsu Univ Wujin Hosp Changzhou Jiangsu Peoples R China

Multienzyme detection and monitoring enzyme activity in situ are significant for the disease to diagnose. This study aims to develop a quantum dots (QDs)-based nanoprobe Cyanine5-DDDLEVLFQFPGLVPRGSGGHHHHHH-QDs (Cy5-LEVLVP-QD), which is able to detect two enzymes inside a bent capillary using CE. Cy5-LEVLVP and QDs were allowed to bind with each other through metal affinity interaction and then injected the Cy5-LEVLVP-QD complex into a capillary with different bends, followed by related enzyme that can cleave the Cy5-LEVLVP peptide. The fluorescence of Cy5 was excited by QDs due to Forster resonance energy transfer. By monitoring the peaks produced by the original Cy5-LEVLVP-QD complex and a significant fluorescence change, sensitive analysis of two different enzymes was conducted. Therefore, the novel approach of using capillaries with semicircular bends could prove particularly useful for enzyme investigating in disease.

关键词： CE enzyme Forster resonance energy transfer peptide quantum dots

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The Northern and Southern Hemispheric Asymmetries of Mesospheric Ozone at High Latitudes During the January 2012 Solar Proton Events

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SPACE WEATHER-THE INTERNATIONAL JOURNAL OF RESEARCH AND APPLICATIONS 2023年第5期21卷 e2023SW003435-e2023SW003435页

作者： Xiong, Shiping Li, Jingyuan Wei, Guanchun Lu, Jianyong Tian, Yufeng Zhang, Xiaoping Fu, Shuai Sun, Meng Li, Zheng Zhang, Hua cui, Jingqi Jiang, shuwen Yang, Chaolei Nanjing Univ Informat Sci & Technol Inst Space Weather Sch Atmospher Phys Nanjing Peoples R China China Geol Survey Kunming Gen Survey Nat Resources Ctr Kunming Peoples R China Macau Univ Sci & Technol State Key Lab Lunar & Planetary Sci Macau Peoples R China CNSA Macau Ctr Space Explorat & Sci Macau Peoples R China

Observations by the Sounding of the Atmosphere using Broadband Emission Radiometry (SABER) and simulations by the Whole Atmosphere Community Climate Model-eXtended (WACCM-X) are used to analyze the impacts of the January 2012 solar proton events (SPEs) on mesospheric ozone at high latitudes. The mesospheric ozone at high latitudes decreased evidently with the increasing proton flux and ionization rates during SPEs. The results of SABER and WACCM-X both showed that maximum mesospheric ozone depletion reached 100% in the North Hemispheric (NH) high latitudes during SPEs while only 40% in the South Hemispheric (SH) high latitudes. The SPEs-caused ozone changes simply occurred below 85 km and the ozone changes observed by SABER above 85 km may be associated with day-to-day variations. WACCM-X simulations showed NOx (N, NO, and NO2) increased over 600% in both the northern and southern high latitudes during SPEs, while HOx (H, OH, and HO2) increased over 300% in the NH high latitudes and only over 30% in the SH high latitudes. HOx is the main ozone-depleting substance because the reactions of NOx are less capable of depleting ozone than the reactions of HOx. Due to the difference in sunlight between the northern and southern hemispheres, ozone, HOx, and NOx exhibited significant northern and southern hemispheric asymmetries. Meanwhile, an increase in ozone after the SPEs was reported but not explained by previous studies. Here it is shown that the decreases in the HOx caused the increases in ozone after the SPEs.

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