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Fluorescence coupled capillary electrophoresis as a strategy for tetrahedron DNA analysis

TALANTA 2021年 228卷 122225页

作者： Hong, Tingting Zheng, Ronghui Qiu, Lin Zhou, shuwen Chao, Hufei Li, Ying Rui, Wen cui, Pengfei Ni, Xinye Tan, Songwen Jiang, Pengju Wang, Jianhao Changzhou Univ Sch Pharm Changzhou 213164 Jiangsu Peoples R China Nanjing Med Univ Affiliated Changzhou Peoples Hosp 2 Changzhou Jiangsu Peoples R China Cent South Univ Xiangya Sch Pharmaceut Sci 172 Tongzipo Rd Changsha 410013 Hunan Peoples R China Changzhou Le Sun Pharmaceut Co Ltd Changzhou 213125 Jiangsu Peoples R China Jiangsu Dawning Pharmaceut Co Ltd Changzhou 213100 Jiangsu Peoples R China Jiangsu Yue Zhi Biopharmaceut Co Ltd Changzhou 213125 Jiangsu Peoples R China

A strategy based on fluorescence coupled capillary electrophoresis (CE-FL) was developed for analyzing tetrahedron DNA (TD) and TD-doxorubicin (DOX) conjugate. Capillary gel electrophoresis exhibited desirable performance for separating TD and DNA strands. Under the optimized conditions, satisfactory repeatability concerning run-to-run and interday repeatability was obtained, and relative standard deviation value of resolution (n = 6) was 0.64%. Furthermore, the combination of CE and fluorescence detection provided a sensitive platform for quantifying TD concentration and calculating the damage degree of TD. The electrophoretograms indicated that CE-FL was a suitable TD assay method with high specificity and sensitivity. In addition, the application of CE-FL for TD fluorescence resonance energy transfer (FRET) research was also explored. Two types of DNA strands were utilized to interfere the formation of TD. The impact of partially complementary chain and completely complementary chain on FRET signal was explored, and the influence mechanism was discussed. After applying CE-FL for characterizing TD, we also combine CE and FRET to analyze TD-DOX conjugate. CE presented a favourable technique to monitor DOX loading and releasing processes. These noteworthy results offered a stepping stone for DNA nanomaterials assay by using CE-FL.

关键词： Doxorubicin Fluorescence coupled capillary electrophoresis Fluorescence resonance energy transfer Tetrahedron DNA

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经肌间隙和椎间孔入路减压植骨融合单侧固定治疗椎管容积狭小腰椎间盘突出症的疗效观察

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中国骨与关节损伤杂志 2025年第3期40卷 258-261页

作者：李忠伟周坤杨新明银乐乐徐翔张树文崔泳新疆医科大学第五附属医院脊柱外科新疆乌鲁木齐830000 新疆维吾尔自治区人民医院骨科中心脊柱外科新疆乌鲁木齐830000

目的观察经肌间隙和椎间孔入路减压植骨融合单侧椎弓根钉内固定治疗椎管容积狭小腰椎间盘突出症的可行性及临床疗效。方法回顾性分析自2019-01—2020-06手术治疗的54例椎管容积狭小腰椎间盘突出症,观察组27例经肌间隙和椎间孔入路减压... 详细信息

目的观察经肌间隙和椎间孔入路减压植骨融合单侧椎弓根钉内固定治疗椎管容积狭小腰椎间盘突出症的可行性及临床疗效。方法回顾性分析自2019-01—2020-06手术治疗的54例椎管容积狭小腰椎间盘突出症,观察组27例经肌间隙和椎间孔入路减压植骨融合单侧椎弓根钉内固定,对照组27例经后正中入路全椎板切除减压植骨融合双侧椎弓根钉内固定。比较两组手术时间、术中出血量、术后2周疼痛VAS评分、术后6个月ODI指数、椎间植骨融合时间。结果54例均获得随访,随访时间9~16个月,平均11.3个月。术中均无神经、血管损伤,观察组1例脑脊液漏,对照组2例脑脊液漏,对症处理。术前责任节段支配肌群肌力为Ⅰ、Ⅱ级的患者肌力恢复至Ⅲ级,其余患者均恢复至正常。观察组手术时间较对照组短,术中出血量少于对照组,术后2周疼痛VAS评分低于对照组,术后6个月ODI指数低于对照组,差异有统计学意义(P<0.05)。观察组椎间植骨融合时间为(12.00±1.14)个月,对照组为(11.07±1.28)个月,组间椎间植骨融合时间差异无统计学意义(P>0.05)。结论经肌间隙和椎间孔入路减压植骨融合单侧椎弓根钉内固定治疗椎管容积狭小的腰椎间盘突出症可以取得满意的疗效,具有手术操作简单、创伤小、安全性较高、并发症少等优点。

关键词：腰椎间盘突出症椎管容积狭小肌间隙入路椎间孔入路植骨融合椎弓根钉单边内固定

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Silica-Coated Fe₃O₄ Nanoparticles as a Bifunctional Agent for Magnetic Resonance Imaging and ZnII Fluorescent Sensing

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TECHNOLOGY IN CANCER RESEARCH & TREATMENT 2021年第20期20卷

作者： Qiu, Lin Zhou, shuwen Li, Ying Rui, Wen cui, Pengfei Zhang, Changli Yu, Yongsheng Wang, Cheng Wang, Xiang Wang, Jianhao Jiang, Pengju Changzhou Univ Sch Pharm Changzhou 213164 Jiangsu Peoples R China Changzhou Univ Sch Med Changzhou 213164 Jiangsu Peoples R China Jiangsu Sci Stand Med Testing Co Ltd Changzhou Jiangsu Peoples R China Nanjing Med Univ Affiliated Changzhou Peoples Hosp 2 Changzhou 213003 Jiangsu Peoples R China Nanjing Xiaozhuang Univ Sch Environm Sci Nanjing 211171 Peoples R China Tongji Univ Shanghai Matern & Infant Hosp 1 Sch Med Clin & Translat Res Ctr Shanghai Peoples R China Changzhou Le Sun Pharmaceut Co Ltd Changzhou 213125 Jiangsu Peoples R China

Bifunctional magnetic/fluorescent core-shell silica nanospheres (MNPs) encapsulated with the magnetic Fe3O4 core and a derivate of 8-amimoquinoline (N-(quinolin-8-yl)-2-(3-(triethoxysilyl) propylamino) acetamide) (QTEPA) into the shell were synthesized. These functional MNPs were prepared with a modified stober method and the formed Fe3O4@SiO2-QTEPA core-shell nanocomposites are biocompatible, water-dispersible, and stable. These prepared nanoparticles were characterized by X-ray power diffraction (XRD), transmission electron microscopy (TEM), thermoelectric plasma Quad II inductively coupled plasma mass spectrometry (ICP-MS), superconducting quantum interference device (SQUID), TG/DTA thermal analyzer (TGA) and Fourier transform infrared spectroscopy (FTIR). Further application of the nanoparticles in detecting Zn2+ was confirmed by the fluorescence experiment: the nanosensor shows high selectivity and sensitivity to Zn2+ with a 22-fold fluorescence emission enhancement in the presence of 10 mu M Zn2+. Moreover, the transverse relaxivity measurements show that the core-shell MNPs have T2 relaxivity (r2) of 155.05 mM(-1) S-1 based on Fe concentration on the 3.0 T scanner, suggesting that the compound can be used as a negative contrast agent for MRI. Further in vivo experiments showed that these MNPs could be used as MRI contrast agent. Therefore, the new nanosensor provides the dual modality of magnetic resonance imaging and optical imaging.

关键词： nanosensor Fe3O4 nanoparticles magnetic resonance imaging core-shell silica nanospheres ZnII fluorescent sensing

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A seamless and iterative DNA assembly method named PS-Brick and its assisted metabolic engineering for threonine and 1-propanol production

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BIOTECHNOLOGY FOR BIOFUELS 2019年第1期12卷 1-20页

作者： Liu, shuwen Xiao, Haihan Zhang, Fangfang Lu, Zheng Zhang, Yun Deng, Aihua Li, Zhongcai Yang, cui Wen, Tingyi Chinese Acad Sci Inst Microbiol CAS Key Lab Pathogen Microbiol & Immunol Beijing 100101 Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China Anhui Univ Inst Phys Sci & Informat Technol Hefei 230039 Anhui Peoples R China Univ Chinese Acad Sci Savaid Med Sch Beijing 100049 Peoples R China

BackgroundDNA assembly is an essential technique enabling metabolic engineering and synthetic biology. Combining novel DNA assembly technologies with rational metabolic engineering can facilitate the construction of microbial cell factories. Amino acids and derived biochemicals are important products in industrial biotechnology with wide application and huge markets. DNA assembly scenarios encountered in metabolic engineering for the construction of amino acid and related compound producers, such as design-build-test-learn cycles, construction of precise genetic circuits and repetitive DNA molecules, usually require for iterative, scarless and repetitive sequence assembly methods, *** endonuclease (RE)-assisted strategies constitute one of the major categories of DNA assembly. Here, we developed a Type IIP and IIS RE-assisted method named PS-Brick that comprehensively takes advantage of the properties of PCR fragments and REs for iterative, seamless and repetitive sequence assembly. One round of PS-Brick reaction using purified plasmids and PCR fragments was accomplished within several hours, and transformation of the resultant reaction product from this PS-Brick assembly reaction exhibited high efficiency (10(4)-10(5) CFUs/mu g DNA) and high accuracy (similar to 90%). An application of metabolic engineering to threonine production, including the release of feedback regulation, elimination of metabolic bottlenecks, intensification of threonine export and inactivation of threonine catabolism, was stepwise resolved in E. coli by rounds of design-build-test-learn cycles through the iterative PS-Brick paradigm, and 45.71g/L threonine was obtained through fed-batch fermentation. In addition to the value of the iterative character of PS-Brick for sequential strain engineering, seamless cloning enabled precise in-frame fusion for codon saturation mutagenesis and bicistronic design, and the repetitive sequence cloning ability of PS-Brick enabled

关键词： DNA assembly Metabolic engineering Design-build-test-learn cycle Threonine 1-Propanol Escherichia coli

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Identification of competitive endogenous RNAs network in breast cancer

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CANCER MEDICINE 2019年第5期8卷 2392-2403页

作者： Wang, Xiaojin Wan, Jiahui Xu, Zhanxiang Jiang, Shijun Ji, Lin Liu, Yutian Zhai, shuwen cui, Rongjun Mudanjiang Med Univ Dept Biochem & Mol Biol Mudanjiang Peoples R China Mudanjiang Med Univ Dept Cardiovasc Med Hongqi Hosp Mudanjiang Peoples R China

Background MiRNAs can regulate gene expression directly or indirectly, and long noncoding RNAs as competing endogenous RNA (ceRNAs) can bind to miRNAs competitively and affect mRNA expression. The ceRNA network is still unclear in breast cancer. In this study, a ceRNA network was constructed, and new treatment and prognosis targets and biomarkers for breast cancer were explored. Methods A total of 1 096 cancer tissues and 112 adjacent normal tissues to cancer from the TCGA database were used to screen out significant differentially expressed mRNAs (DEMs), lncRNAs (DELs), and miRNAs (DEMis) to construct a ceRNA network. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were used to predict potential functions. Survival analysis was performed to predict which functions were significant for prognosis. Results From the analysis, 2 139 DEMs, 1 059 DELs, and 84 DEMis were obtained. Targeting predictions for DEMis-DELs and DEMis-DEMs can yield 26 DEMs, 90 DELs, and 18 DEMis. We performed GO enrichment analysis, and the results showed that the upregulated DEMs were involved in nucleosomes, extracellular regions, and nucleosome assembly, while the downregulated DEMs were mainly involved in Z disk, muscle contraction, and structural constituents of muscle. KEGG pathway analysis was performed on all DEMs, and the pathways were enriched in retinol metabolism, steroid hormone biosynthesis, and tyrosine metabolism. Through survival analysis of the ceRNA network, we identified four DEMs, two DELs, and two DEMis that were significant for poor prognosis. Conclusions This study suggested that constructing a ceRNA network and performing survival analysis on the network could screen out new significant treatment and prognosis targets and biomarkers.

关键词： breast cancer ceRNA network GO enrichment analysis KEGG pathway analysis prognosis survival analysis

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赤霞珠葡萄籽多酚低共熔溶剂提取及其抗氧化活性研究

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中国酿造 2021年第8期40卷 110-116页

作者：崔梦情石侃邓声林曹嘉敏刘树文西北农林科技大学葡萄酒学院陕西杨凌712100 西北农林科技大学合阳葡萄试验示范站陕西渭南715300 陕西省葡萄与葡萄酒工程技术研究中心陕西杨凌712100

研究不同绿色低共熔溶剂(DESs)结合超声辅助对赤霞珠葡萄皮渣中葡萄籽多酚提取及提取物体外抗氧化活性的影响。结果表明,部分DESs对总酚及总黄酮的提取量显著优于传统提取溶剂(水、体积分数为80%乙醇、体积分数为80%甲醇)(P<0.05)。... 详细信息

研究不同绿色低共熔溶剂(DESs)结合超声辅助对赤霞珠葡萄皮渣中葡萄籽多酚提取及提取物体外抗氧化活性的影响。结果表明,部分DESs对总酚及总黄酮的提取量显著优于传统提取溶剂(水、体积分数为80%乙醇、体积分数为80%甲醇)(P<0.05)。提取总酚和总黄酮最优的DESs分别为氯化胆碱-乳酸和脯氨酸-乙酰丙酸。绝大部分DESs提取物中单体酚的含量显著高于传统提取溶剂(P<0.05)。氯化胆碱-乙二醇提取物DPPH自由基清除能力最强,脯氨酸-乙二醇提取物ABTS+自由基清除能力最强,均显著高于传统溶剂提取物(P<0.05)。皮尔森相关性分析结果表明,赤霞珠葡萄籽DESs提取物中总酚、儿茶素、表儿茶素含量与其体外抗氧化能力呈显著正相关(P<0.05)。

关键词：葡萄籽低共熔溶剂超声辅助提取多酚抗氧化活性

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Crosslinked microporous polyarylate membranes with high Kr/Xe separation performance and high stability under irradiation

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JOURNAL OF MEMBRANE SCIENCE 2020年 611卷

作者： Yu, shuwen Li, Shichun Wang, Huazhao Zhu, Chunhua Hou, Jingwei cui, Sheng Shen, Xiaodong Liu, Yu China Acad Engn Phys Inst Chem Mat Mianyang 621900 Sichuan Peoples R China Nanjing Tech Univ Coll Mat Sci & Engn Nanjing 210009 Peoples R China Nanjing Tech Univ Jiangsu Collaborat Innovat Ctr Adv Inorgan Funct Nanjing 210009 Peoples R China Chinese Acad Sci Haixi Inst Xiamen Inst Rare Earth Mat Xiamen 361021 Peoples R China Univ Cambridge Dept Mat Sci & Met Cambridge CB3 0FS England Univ Queensland Sch Chem Engn Brisbane Qld 4072 Australia

The Kr/Xe separation based on membranes is of great significance for radioactive Kr-85 capture from nuclear fuel reprocessing plants and expensive Xe production, thus the membrane material that has both high separation performance and stability is highly sought after. Herein, the crosslinked polymeric micro-porous polyarylate (PAR) membrane was synthesized by interfacial polymerization, and its Kr/Xe separation performance and stability under various conditions including gamma irradiation were investigated. The PAR membrane displays Kr permeance of 0.22 GPU and Kr/Xe selectivity of 3.6 tested with mixed gas at room temperature, which is better than other reported polymeric membranes and even better than the amorphous carbon membrane. After the gamma irradiation, the PAR can preserve its chemical structures, while the physical ageing is accelerated, which leads to only 25% decrease in Kr/Xe selectivity after 20 kGy irradiation. This work demonstrates that the micro-porosity and the crosslinking structures play crucial roles in improving both separation performance and stability of polymeric membranes for radioactive Kr/Xe separation.

关键词： Crosslink Microporous polymer membrane Kr/Xe separation Irradiation Ageing

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Structure-aided optimization of 3-O-β-chacotriosyl ursolic acid as novel H5N1 entry inhibitors with high selective index

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BIOORGANIC & MEDICINAL CHEMISTRY 2019年第18期27卷 4048-4058页

作者： Liao, Yixian Chen, Lizhu Li, Sumei cui, Zi-ning Lei, Zhiwei Li, Hui Liu, shuwen Song, Gaopeng South China Agr Univ Coll Mat & Energy Guangzhou 510642 Guangdong Peoples R China South China Agr Univ Guangdong Prov Key Lab Microbial Signals & Dis Co Integrat Microbiol Res Ctr State Key Lab Conservat & Utilizat Subtrop Agrobi Guangzhou 510642 Guangdong Peoples R China Southern Med Univ Sch Pharmaceut Sci Guangzhou 510515 Guangdong Peoples R China Jinan Univ Sch Med Dept Human Anat Guangzhou 510632 Guangdong Peoples R China Guizhou Acad Agr Sci Guizhou Tea Reasearch Inst Guiyang 550006 Guizhou Peoples R China

Currently, entry inhibitors contribute immensely in developing a new generation of anti-influenza virus drugs. Our earlier studies have identified that 3-O-beta-chacotriosyl ursolic acid (1) could inhibit H5N1 pseudovirus by targeting hemagglutinin (HA). In the present study, a series of C-28 modified pentacyclic triterpene saponins via conjugation with a series of amide derivatives were synthesized and their antiviral activities against influenza A/Duck/Guangdong/99 virus (H5N1) in MDCK cells were evaluated. The SARs analysis of these compounds revealed that introduction of certain amide structures at the 17-COOH of ursolic acid could significantly enhance both their antiviral activity and selective index. This study indicated that the attachment of the methoxy group or Cl atom to the phenyl ring at the ortho- or para-position was crucial to improve inhibitory activity. Mechanism studies demonstrated that these title triterpenoids could bind tightly to the viral envelope HA to block the attachment of viruses to host cells, which was consistent with docking studies.

关键词： 3-O-beta chacotriosyl pentacyclic triterpenoids H5N1 entry inhibitors Structure-activity relationships

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Polysaccharides extract from Vaccaria segetalis seeds inhibits kidney infection by regulating cathelicidin expression

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JOURNAL OF ETHNOPHARMACOLOGY 2021年 267卷 113505页

作者： Mao, Xin Yao, Rongmei Guo, Hongling Bao, Lei Bao, Yanyan Xu, Yingli Sun, Jing Guo, Shanshan Shi, Yujing Liu, shuwen Zhang, Haijiang cui, Xiaolan China Acad Chinese Med Sci Inst Chinese Mat Med Beijing 100700 Peoples R China Huaiyin Inst Technol Jiangsu Key Lab Reg Resource Exploitat & Med Res Huaian 223003 Peoples R China Huaian Socal Technol Co Ltd Huaian 223003 Peoples R China Southern Med Univ Sch Pharmaceut Sci Guangzhou 510515 Peoples R China North China Univ Sci & Technol Coll Tradit Chinese Med Qinhuangdao 063210 Hebei Peoples R China Peking Univ Hosp 6 Inst Mental Hlth NHC Key Lab Mental Hlth Beijing 100191 Peoples R China Peking Univ Hosp 6 Natl Clin Res Ctr Mental Disorders Beijing 100191 Peoples R China

Ethnopharmacological relevance: According to the Chinese Pharmacopoeia, the seeds of Vaccaria segetalis, a traditional medicinal herb, can be used for treating urinary diseases. The polysaccharides extract from V. segetalis seeds (VSP) has been shown to prevent urinary tract infections (UTIs). Aim of the study: Investigate the effects of VSP on treating kidney infection induced by uropathogenic Escherichia coli (UPEC) and the underlying mechanisms. Materials and methods: Both in vivo and in vitro infection models were established with the UPEC strain CFT073. After oral administration of VSP, the levels of bacterial load, cathelicidin (CRAMP), Toll-like receptors (TLRs) in the kidney were evaluated. The expression of cathelicidin (LL-37) in human renal cell carcinoma cell line (A498) was tested after the treatment of VSP. Results: In the kidneys of infection models, high-titer bacteria was detected. In the kidney of rat model, the expression of CRAMP was down-regulated, no significant change was observed in the levels of TLRs. After oral administration of VSP, the bacterial load was significantly decreased in rat and mouse models, and the levels of CRAMP and TLRs were significantly up-regulated in rat model. In vitro, the expression of LL-37 was significantly inhibited by CFT073. VSP up-regulated the expression of LL-37 in A498 cells. Conclusions: The up-regulation of cathelicidin expression may contribute to the therapeutic effects of VSP on kidney infection.

关键词： Kidney infection Vaccaria segetalis Cathelicidin CRAMP LL-37

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Normal incidence polarization independence, oblique incidence large angle insensitivity broadband plasmonic absorber from Ultraviolet to near infrared 11

Normal incidence polarization independence, oblique incidenc...

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11th International Conference on Information Optics and Photonics (CIOP)

作者： Gao, Huixuan Peng, Wei cui, Wenli Chu, shuwen Yu, Li Yang, Xiong Jing, Zhenguo Dalian Univ Technol Sch Phys Dalian 116024 Peoples R China

ISBN: (数字)9781510631748

ISBN: (纸本)9781510631748

In this paper, we theoretically design and numerically verify a broadband plasmonic absorber that works continuously in ultraviolet to near-infrared region. Different from the traditional metal-insulator-metal (MIM) three-layer structure, our perfect absorber is based on insulator-metal-insulator-metal (IMIM) four-layer structure. This perfect absorber has 280 nm ultra-thin thickness, and the combination of refractory metal titanium nitride and high-melting-point insulator silica gives our absorber strong thermal stability. The novel titanium nitride ring-square array layer combines the absorption of different wavelength bands so that the absorber can achieve a continuous absorption of more than 90% from wavelength 200 to 1200 nm. Finite-difference time-domain (FDTD) calculated average absorption rate reaches 94.85%, which 99.40% maximum absorption at wavelength 270 nm and 90.30% minimum absorption at 390 nm. In addition, polarization independence under normal incidence and large incident angle insensitivity under oblique incidence, making our perfect absorber more advantageous in applications such as solar energy collection, photothermal conversion, and invisibility cloak.

关键词： Broadband absorber Polarization independence Large incident angle insensitivity Plasmonic

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