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华西医学 2021年第8期36卷 1048-1055页

作者：崔文耀何文博滕海波鞠延吴书文朱丽徐建国刘志勇四川大学华西医院神经外科/四川大学华西护理学院成都610041 四川大学华西医院神经外科成都610041

目的探讨儿童弥漫性脑干胶质瘤的临床特点及预后因素。方法回顾性分析四川大学华西医院2016年1月—2019年5月经病理确诊的儿童弥漫性脑干胶质瘤,纳入患儿人口学资料、临床表现、MRI表现、病理结果、治疗方法等因素进行预后研究。结果共... 详细信息

目的探讨儿童弥漫性脑干胶质瘤的临床特点及预后因素。方法回顾性分析四川大学华西医院2016年1月—2019年5月经病理确诊的儿童弥漫性脑干胶质瘤,纳入患儿人口学资料、临床表现、MRI表现、病理结果、治疗方法等因素进行预后研究。结果共纳入病理确诊的弥漫性脑干胶质瘤患儿39例,其中男21例,女18例;年龄3~14岁,平均(8.1±2.8)岁,以5~10岁者居多(29例);肿瘤横切面最大直径为(4.46±0.81)cm;完全包绕基底动脉16例;并发脑积水15例;中位生存期6个月,1年生存率为15.4%,2年生存率为5.1%。单因素分析表明病变强化及完全包绕基底动脉的患儿生存时间相对较短(P<0.05)。多因素Cox回归分析表明肿瘤是否完全包绕基底动脉与总体生存时间密切相关[风险比=4.596,95%置信区间(1.839,11.488),P=0.001]。结论儿童弥漫性脑干胶质瘤多见于5~10岁患儿,预后差,肿瘤完全包绕基底动脉与总体生存时间短密切相关。

关键词：儿童弥漫性胶质瘤脑干临床特点预后因素

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雪峰山科学钻先导孔发现承压热水层和优质烃源岩

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中国地质 2020年第4期47卷 1268-1269页

作者：崔建军施炜董树文张岳桥严加永孙东升曲玮王克营李淼中国地质科学院地质力学研究所北京100081 自然资源部古地磁与古构造重建重点实验室中国地质科学院地质力学研究所北京100081 南京大学地球科学与工程学院江苏南京210093 中国地质科学院北京100037 湖南煤炭地质勘查院湖南长沙410014

1研究目标(Objective)雪峰山位于扬子克拉通的南缘,经历过多期构造叠加与改造。SinoProbe项目的深反射地震探测结果显示,雪峰山地区具有特殊的地壳结构,可能存在隐伏的古老造山带。同时,雪峰山地区也是寻找页岩气和地热资源的有利地区... 详细信息

1研究目标(Objective)雪峰山位于扬子克拉通的南缘,经历过多期构造叠加与改造。SinoProbe项目的深反射地震探测结果显示,雪峰山地区具有特殊的地壳结构,可能存在隐伏的古老造山带。同时,雪峰山地区也是寻找页岩气和地热资源的有利地区。为探索雪峰山地壳结构特征(为科学深钻提供依据),评价深部资源和能源潜力,本项目组在地表地质调查和反射地震探测的基础上,部署实施了雪峰山先导孔钻探。

关键词：构造叠加地热资源先导孔反射地震地壳结构科学深钻地表地质优质烃源岩

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Efficient CRISPR-Cas9 mediated multiplex genome editing in yeasts

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BIOTECHNOLOGY FOR BIOFUELS 2018年第1期11卷 1-16页

作者： Wang, Laiyou Deng, Aihua Zhang, Yun Liu, shuwen Liang, Yong Bai, Hua cui, Di Qiu, Qidi Shang, Xiuling Yang, Zhao He, Xiuping Wen, Tingyi Chinese Acad Sci Inst Microbiol CAS Key Lab Pathogen Microbiol & Immunol Beijing 100101 Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China Chinese Acad Sci Inst Microbiol CAS Key Lab Microbial Physiol & Metab Engn Beijing 100101 Peoples R China Univ Chinese Acad Sci Savaid Med Sch Beijing 100049 Peoples R China

Background: The thermotolerant methylotrophic yeast Ogataea polymorpha has been regarded as an important organism for basic research and biotechnological applications. It is generally recognized as an efficient and safe cell factory in fermentative productions of chemicals, biofuels and other bio-products. However, it is difficult to genetically engineer for the deficiency of an efficient and versatile genome editing technology. Results: In this study, we developed a CRISPR-Cas9-assisted multiplex genome editing (CMGE) approach including multiplex genes knock-outs, multi-locus (ML) and multi-copy (MC) integration methods in yeasts. Based on CMGE, various genome modifications, including gene deletion, integration, and precise point mutation, were performed in O. polymorpha. Using the CMGE-ML integration method, three genes TAL from Herpetosiphon aurantiacus, 4CL from Arabidopsis thaliana and STS from Vitis vinifera of resveratrol biosynthetic pathway were simultaneously integrated at three different loci, firstly achieving the biosynthesis of resveratrol in O. polymorpha. Using the CMGE-MC method,similar to 10 copies of the fusion expression cassette P-ScTEF1-TAL-P-ScTPI1-4CL-P-ScTEF2-STS were integrated into the genome. Resveratrol production was increased similar to 20 fold compared to the one copy integrant and reached 97.23 +/- 4.84 mg/L. Moreover, the biosynthesis of human serum albumin and cadaverine were achieved in O. polymorpha using CMGE-MC to integrate genes HSA and cadA, respectively. In addition, the CMGE-MC method was successfully developed in Saccharomyces cerevisiae. Conclusions: An efficient and versatile multiplex genome editing method was developed in yeasts. The method would provide an efficient toolkit for genetic engineering and synthetic biology researches of O. polymorpha and other yeast species.

关键词： CRISPR-Cas9-assisted multiplex genome editing Markerless multi-locus integration Markerless multi-copy integration Ogataea polymorpha Saccharomyces cerevisiae

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A Novel Tool for Microbial Genome Editing Using the Restriction-Modification System

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ACS SYNTHETIC BIOLOGY 2018年第1期7卷 98-106页

作者： Bai, Hua Deng, Aihua Liu, shuwen cui, Di Qiu, Qidi Wang, Laiyou Yang, Zhao Wu, Jie Shang, Xiuling Zhang, Yun Wen, Tingyi Chinese Acad Sci Inst Microbiol CAS Key Lab Pathogen Microbiol & Immunol Beijing 100101 Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China Univ Chinese Acad Sci Savaid Med Sch Beijing 100049 Peoples R China

Scarless genetic manipulation of genomes is an essential tool for biological research. The restriction modification (R-M) system is a defense system in bacteria that protects against invading genomes on the basis of its ability to distinguish foreign DNA from self DNA. Here, we designed an R-M system-mediated genome editing (RMGE) technique for scarless genetic manipulation in different microorganisms. For bacteria with Type IV REase, an RMGE technique using the inducible DNA methyltransferase gene, bceSIIM (RMGE-bceSIIM), as the counter-selection cassette was developed to edit the genome of Escherichia coli. For bacteria without Type IV REase, an RMGE technique based on a restriction endonuclease (RMGE-mcrA) was established in Bacillus subtilis. These techniques were successfully used for gene deletion and replacement with nearly 100% counter-selection efficiencies, which were higher and more stable compared to conventional methods. Furthermore, precise point mutation without limiting sites was achieved in E. coli using RMGE-bceSIIM to introduce a single base mutation of A128C into the rpsL gene. In addition, the RMGE-mcrA technique was applied to delete the CANT gene in Saccharomyces cerevisiae DAY414 with 100% counter-selection efficiency. The effectiveness of the RMGE technique in E. coli, B. subtilis, and S. cerevisiae suggests the potential universal usefulness of this technique for microbial genome manipulation.

关键词： scarless genome editing counter-selection cassette restriction-modification (R-M) system Escherichia coli Bacillus subtilis Saccharomyces cerevisiae

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Extraordinary Optical Transmission Performances of Nanosandwiched Grating for Wideband Multi-Function Integration

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PLASMONICS 2017年第5期12卷 1281-1288页

作者： cui, Wenli Chu, shuwen Yu, Li Liang, Yuzhang Peng, Wei Dalian Univ Technol Coll Phys & Optoelect Engn Dalian 116024 Peoples R China North Univ China Coll Sci Taiyuan 030051 Shanxi Peoples R China

In this paper, we present a peculiar metal-dielectric-metal (MDM) nanosandwich grating structure that can achieve extraordinary optical transmission performances at normal incidence in the ultraviolet-visible-near infrared (UV-VIS-NIR) regions. The proposed structure shows three obvious spectrum characteristics: it can obtain high transmittance up to 80 % in NUV region and efficiently blocking visible wavelengths for transverse-magnetic (TM) polarized incidence;a broadband NIR polarizer can be inspired in the wavelength range from 950 to 1400 nm;more surprisingly, these performances do not deteriorated until 30 degrees tilting angle. Compared to other grating structures with single metal overlayer, it shows wider band-stop characteristics and higher broadband transmission transmittance and extinction ratio (ER) in the investigated wavebands. We analyze the underlying physical mechanism by using numerical simulation, which is primarily attributed to metal ultraviolet transparency, surface plasmon polariton (SPP) at metal/dielectric interface, Fabry-Perot (FP)-like cavity mode within this dielectric grating, and optical magnetic resonance especially in the dielectric interlayer of the MDM sandwiched structure. This structure is very important for developing high-performance subwavelength multifunctional integrated optical devices.

关键词： Extraordinary optical transmission Nanosandwich grating FP-like cavity mode Surface plasmon polaritons Magnetic resonance

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Boosting Water Dissociation Kinetics on Pt-Ni Nanowires by N-Induced Orbital Tuning

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ADVANCED MATERIALS 2019年第16期31卷 e1807780页

作者： Xie, Yufang Cai, Jinyan Wu, Yishang Zang, Yipeng Zheng, Xusheng Ye, Jian cui, Peixin Niu, shuwen Liu, Yun Zhu, Junfa Liu, Xiaojing Wang, Gongming Qian, Yitai Univ Sci & Technol China Dept Chem Hefei Natl Lab Phys Sci Microscale Hefei 230026 Anhui Peoples R China Univ Sci & Technol China Natl Synchrotron Radiat Lab Hefei 230029 Anhui Peoples R China Chinese Acad Sci Inst Soil Sci Key Lab Soil Environm & Pollut Remediat Nanjing 210008 Jiangsu Peoples R China

Although it is commonly believed that the water-dissociation-related Volmer process is the rate-limiting step for alkaline hydrogen evolution reaction (HER) on Pt-based catalysts, the underlying essence, particularly on the atomic scale, still remains unclear. Herein, it is revealed that the sluggish water-dissociation behavior probably stems from unfavorable orbital orientation and the kinetic issue is successfully resolved via N-induced orbital tuning. Impressively, N modified Pt-Ni nanowires deliver an ultralow overpotential of 13 mV at 10 mA cm(-2), which represents a new benchmark for alkaline HER catalysis. Fine-structural characterization and density functional theory analysis illustrate that the introduced nitrogen can uniquely modulate the electron densities around the Ni sites, and further create empty d(z)(2) orbitals with superior orientation for water adsorption and activation. More importantly, it is demonstrated that N-induced orbital modulation can generally boost the alkaline HER activities of Pt-Co, Pt-Ni, and Pt-Cu, offering a new perspective for the design of HER catalysts and beyond.

关键词： electrocatalysts hydrogen evolution reaction orbital tuning Pt-Ni nanowires water dissociation kinetics

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Systematic study of cluster encapsulated in carbon nanotubes

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Journal of Computational and Theoretical Nanoscience 2015年第11期12卷 4267-4271页

作者： cui, shuwen Wang, Xiaosong Zhu, Ruzeng Department of Physics and Electronic Information Cangzhou Normal University Cangzhou061001 China Institute of Mechanical and Power Engineering Henan Polytechnic University Jiaozuo Henan Province454000 China Key Laboratory of Microgravity Institute of Mechanics Chinese Academy of Science Beijing100190 China

We systematically study the structure of argon cluster in the single-walled carbon nanotubes by molecular dynamics simulation. The effects of the diameters and lengths of the single-walled carbon nanotubes (SWCNTs), temperature on the equilibrium structure of argon cluster, fixed and flexible SWCNTs in the simulations are analyzed. As the diameters of SWCNTs with different chiralities increase at a fixed temperature 20 K, the equilibrium structures of the argon cluster transform from a monoatomic chain to helical and then to multishell coaxial cylinders. It is almost no noticeable influence on the final equilibrium configurations whether the carbon nanotubes is fixed or flexible. The effect of the length of single-walled carbon nanotube on equilibrium configuration at given temperature are also studied by molecular dynamics simulation. © 2015 American Scientific Publishers.

关键词： Molecular dynamics

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Covalently bonded zeolitic imidazolate frameworks and polymers with enhanced compatibility in thin film nanocomposite membranes for gas separation

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JOURNAL OF MEMBRANE SCIENCE 2017年 540卷 155-164页

作者： Yu, shuwen Li, Shichun Huang, Shiliang Zeng, Zehua cui, Sheng Liu, Yu China Acad Engn Phys Inst Chem Mat Mianyang 621900 Peoples R China Nanjing Tech Univ Coll Mat Sci & Engn Nanjing 210009 Jiangsu Peoples R China Nanjing Tech Univ Jiangsu Collaborat Innovat Ctr Adv Inorgan Funct Nanjing 210009 Jiangsu Peoples R China

Mixed matrix membranes (MMMs) for gas separation show promising applications in energy and environment related fields. However, the poor compatibility between particles and polymers in MMMs is the main problem. Zeolitic imidazolate frameworks (ZIFs) as inorganic-organic hybrid porous materials have better compatibility with polymers than other inorganic particles, but interfacial defects between ZIFs and polymers still have been observed in MMMs. In this work, the interfacial compatibility in MMMs has been enhanced by building covalent bonds between amine modified ZIF-8 (NH2-ZIF-8) and polyamide in thin film nanocomposite (TFN) membranes prepared by in situ interfacial polymerization (IP). The attendance of amino groups on the surface of NH2-ZIF-8 was confirmed by both experiments and molecular simulation. The covalent bonds formed between NH2-ZIF-8 and trimesoyl chloride (TMC), the monomer in organic phase of IP, were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). Compared with the TFN membrane with ZIF-8, the TFN membrane with NH2-ZIF-8 exhibits both higher CO2 permeance and higher CO2/N-2 selectivity, which is mainly attributed to the enhanced interfacial compatibility and disturbed chain segment of polyamide by covalent bonds. Moreover, at high content of NH2-ZIF-8 in membranes, no interfacial crack appears between NH2-ZIF-8 and polyamide in membranes, which also confirms the excellent compatibility between NH2-ZIF-8 and polyamide. The TFN membrane with NH2-ZIF-8 shows high separation performance for CO2/N-2, CO2/NO, and CO2/He gas pairs, which surpasses the Robeson's upper bounds.

关键词： Zeolitic imidazolate frameworks (ZIFs) Thin film nanocomposite (TFN) membranes Compatibility Covalent bonds Gas separation

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松散型花椰菜新品种平研85的选育

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长江蔬菜 2021年第12期 46-47页

作者：薛丽朋马建国崔新霞杨树文刘扬扬李菁菁牛文珊王君焕曹永麒石家庄平华农业科学研究所河北050000

平研85是以雄性不育系NH617A为母本、以自交不亲和系YZ159为父本配制而成的中晚熟松散型花椰菜一代杂种。秋季从定植至收获80~85 d,春季60 d左右。株型半直立,生长势强,叶片长椭圆形,蓝绿色;花球半圆形,白色,球面整齐,花梗绿,口感甜脆,... 详细信息

平研85是以雄性不育系NH617A为母本、以自交不亲和系YZ159为父本配制而成的中晚熟松散型花椰菜一代杂种。秋季从定植至收获80~85 d,春季60 d左右。株型半直立,生长势强,叶片长椭圆形,蓝绿色;花球半圆形,白色,球面整齐,花梗绿,口感甜脆,品质佳,单球质量1.5~2.0 kg,常规栽培每667 m^(2)平均产量在3000 kg以上。适宜在天津、河北、山东、北京、河南中北部等地区春秋两季种植,河北坝上地区及甘肃、青海春季种植。

关键词：松散型花椰菜平研85 选育雄性不育系一代杂种

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Effects of prey of different nutrient quality on elemental nutrient budgets in Noctiluca scintillans

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SCIENTIFIC REPORTS 2017年第1期7卷 1-12页

作者： Zhang, shuwen Liu, Hongbin Glibert, Patricia M. Guo, cui Ke, Ying Hong Kong Univ Sci & Technol Div Life Sci Kowloon Hong Kong Peoples R China Univ Maryland Ctr Environm Sci Horn Point Lab POB 775 Cambridge MD 21613 USA

Noctiluca scintillans (Noctiluca) is a cosmopolitan red tide forming heterotrophic dinoflagellate. In this study, we investigated its ingestion, elemental growth yield and excretion when supplied with different quality food (nutrient-balanced, N-limited and P-limited). Total cellular elemental ratios of Noctiluca were nearly homeostatic, but the ratio of its intracellular NH4+ and PO43- was weakly regulated. Noctiluca thus seems able to differentially allocate N and P to organic and inorganic pools to maintain overall homeostasis, and it regulated its internal N more strongly and efficiently than P. The latter was substantiated by its comparatively stable C:N ratio and compensatory feeding on N-limited prey. Using both starvation experiments and mass balance models, it was found that excretion of C, N, and P by Noctiluca is highly affected by prey nutritional quality. However, based on modeling results, nutrients seem efficiently retained in actively feeding Noctiluca for reproduction rather than directly released as was shown experimentally in starved cells. Moreover, actively feeding Noctiluca tend to retain P and preferentially release N, highlighting its susceptible to P-limitation. Recycling of N and P by Noctiluca may supply substantial nutrients for phytoplankton growth, especially following bloom senescence.

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