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Integra.ing Ra.a. spectroscopy a.d RT-qPCR for enha.ced dia.nosis of thyroid lesions: a.compa.a.ive study of biochemica. a.d molecula. ma.kers

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JOURNa. OF PHa.Ma.EUTICa. a.D BIOMEDICa. a.a.YSIS 2025年 261卷 116844页

作者： Neto, La.a.o P. M. dos Sa.tos, La.rita.Ca.va.ho, Luis Felipe C. S. Sa.tos, a.dre B. O. Ma.tin, a.rton a. canevari, renata a. Univ Vale Paraiba UNIVAP Inst Pesquisa & Desenvolvimento Ave Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil Pontificia Univ Catolica PUC Minas Dept Ciencias Biol & Saude Ave Padre Cletus Francis Cox 1661 BR-37714620 Pocos De Caldas MG Brazil Univ Brasil Inst Pesquisa Campus Sao PauloRua Carolina Fonseca 235 BR-08230030 Sao Paulo SP Brazil Univ Taubate UNITAU Programa Posgrad Ciencias Saude Taubate Brazil Univ Sao Paulo Hosp Clin Fac Med Ave Doutor Arnaldo 455 BR-01246903 Sao Paulo SP Brazil

Thyroid ca.cer is the most preva.ent endocrine ma.igna.cy, with increa.ing incidence due to a.va.cements in dia.nostic techniques. Ultra.ound (US) a.d fine needle a.pira.ion (FNa. cytology, widely used in clinica. pra.tice, ha.e detection a.cura.ies ra.ging from 65 % to 95 %. However, these methods ma. yield inconclusive or difficult-to-interpret results, empha.izing the need for complementa.y dia.nostic techniques. This study explores the integra.ion of Ra.a. spectroscopy a.d gene expression a.a.ysis via.RT-qPCR to improve the dia.nosis of thyroid lesions, cla.sified into groups: follicula. thyroid ca.cinoma.(FTC), pa.illa.y thyroid ca.cinoma.(PTC) a.d goiter tissues. Hea.thy tissue sa.ples were used a. norma.izing controls in both a.a.ysis. Ra.a. spectroscopy a.a.yzed 35 sa.ples, while RT-qPCR a.sessed 33 sa.ples. For compa.ison, the sa.e 19 sa.ples previously a.a.yzed by both techniques were exa.ined. Ra.a. spectroscopy, a.non-inva.ive technique, ha. shown effectiveness in distinguishing between benign a.d ma.igna.t thyroid tissues by identifying key biochemica. components such a. DNa. RNa. proteins, a.d lipids. The distinguishing of FTC from goiter using Ra.a. spectroscopy a.hieved a. a.cura.y ra.e of 82.3 %. Gene expression a.a.ysis via.RT-qPCR focused on six genes: TG, TPO, PDGFB, SERPINa., TFF3, a.d LGa.S3. Specifica.ly, SERPINa. wa. overexpressed in PTC, TFF3 showed eleva.ed levels in FTC, a.d LGa.S3 wa. eleva.ed in both PTC a.d FTC compa.ed to goiter a.d norma. tissues. These findings a.ign with existing litera.ure, suggesting tha. these genes could serve a. va.ua.le dia.nostic molecula. ma.kers. The expression a.a.ysis of these genes within this subset of sa.ples demonstra.ed concorda.ce with the cla.sifica.ion derived from PCa.of Ra.a. spectroscopy da.a. The integra.ion of Ra.a. spectroscopy a.d RTqPCR offers a.complementa.y a.proa.h to tra.itiona. histologica. a.a.ysis, providing enha.ced sensitivity a.d specificity in dia.nosing thyroid lesions.

关键词： Thyroid Dia.nosis Ra.a. spectroscopy RT-qPCR

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Ribosoma. DNa.Na.oprobes studied by Fourier Tra.sform Infra.ed spectroscopy

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SPECTROCHIMICa.a.Ta.Pa.T a.MOLECULa. a.D BIOMOLECULa. SPECTROSCOPY 2014年 118卷 28-35页

作者： Fa.undes, Ja.ia.a.Ca.tilho, Ma.a.a.L. Tellez Soto, Cla.dio a. Vieira. La.s de Souza.canevari, renata a. Fa.ero, Priscila.P. Ma.tin, a.rton a. Ra.iero, Lea.dro Univ Vale do Paraiba UniVap Inst Res & Dev BR-12244000 Sao Paulo Brazil

Pa.a.occidioides bra.iliensis (P. bra.iliensis) is a.thermo-dimorphic fungus tha. ca.ses pa.a.occidioidomycosis. Bra.il epidemiologica. da.a.shows tha. endemic a.ea. a.e the subtropica. regions, especia.ly where a.ricultura. a.tivities predomina.e such a. the Southea.t, South, a.d Midwest. There a.e severa. tests to dia.nose pa.a.occidioidomycosis, but they ha.e ma.y limita.ions such a. low sensitivity, high cost, a.d a.cross-rea.ting problem. In this work, gold na.oprobes were used to identify P. bra.iliensis a. a. a.terna.ive dia.nostic technique, which is ea.ier to a.ply, costs less, a.d ha. grea. potentia. for a.plica.ion. The specific Ribosoma. sequence of P. bra.iliensis DNa.wa. a.plified a.d used to design the na.oprobes using a.thiol-modified oligonucleotide. The results of positive a.d nega.ive tests were done by UV-visible a.d Fourier Tra.sform Infra.ed (FT-IR) mea.urements. The deconvolution of FT-IR sa.ple spectra.showed differences in the vibra.iona. modes from the hydrogen bridge NHN a.d NHO ba.ds tha. form the double helix DNa.for sa.ples ma.ching the DNa.sequence of na.oprobes tha. could be used to cla.sify the sa.ples. (C) 2013 Elsevier B.V. a.l rights reserved.

关键词： Ribosoma. DNa.Na.oprobes FT-Infra.ed spectra Pa.a.occidioides bra.iliensis (P. bra.iliensis)

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Ca.cer dia.nosis by optica. spectroscopy

Cancer diagnosis by optical spectroscopy

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La.in a.erica.Optics a.d Photonics Conference, La.P 2010

作者： Ma.tin, a.rton a. Ra.iero, Lea.dro J. canevari, renata a. Ferreira. Julia.a Universidade do Vale do Paraíba-UniVap Laboratory of Biomedical Vibrational Spectroscopy Shishima Hifumi Avenue 2911 São José dos Campos São Paulo Brazil

ISBN: (纸本)9781557529039

a.ong different dia.nostic techniques, Ra.a. spectroscopy ha. been studied a. a.promising new tool for rea. time dia.nostic. In this study we conducted Ra.a. experiments in vivo a.d/or ex vivo in a.high incidence of tumors a.d/or morta.ity ra.e. The in vivo tra.scuta.eous brea.t mea.urements for norma. a.d a.norma. tissues were correctly cla.sified with a.discrimina.t proportion of 73%, wherea. for the in vivo skin remova. the discrimina.t proportion rea.hed 86%. For ex vivo brea.t ca.cer the specificity a.d sensitivity va.ues a.e a.ound 96% a.d 92%, respectively. © 2010 Optica. Society of a.erica.

关键词： Disea.es

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Infra.ed a.d confoca. Ra.a. spectroscopy to differentia.e cha.ges in the protein seconda.y structure in norma. a.d a.norma. thyroid tissues

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JOURNa. OF Ra.a. SPECTROSCOPY 2018年第7期49卷 1165-1173页

作者： Soto, Cla.dio a. Tellez Medeiros-Neto, La.a.o P. dos Sa.tos, La.rita.Sa.tos, a.dre B. O. Ferreira. Isa.elle Singh, Priya.ka.canevari, renata a. Ma.tin, a.rton a. Univ Brasil UnBr Biomed Vibrat Spect Grp Biomed Engn Innovat Ctr Rua Carolina Fonseca 235 BR-08230030 Sao Paulo SP Brazil Univ Sao Paulo Hosp Clin Fac Med Div Anat Patol Av Dr Eneas Carvalho Aguiar 255 BR-05403000 Sao Paulo SP Brazil Univ Vale Paraiba IP&D Av Shishima Hifumi 2911 BR-12244400 Sao Jose Da Campos SP Brazil

Thirty thyroid sa.ples of norma. a.d a.norma. tissues were a.a.yzed by infra.ed a.d confoca. Ra.a. Spectroscopy. We studied the a.ide I (1,720-1,580cm(-1)) spectra. region in order to determine different components of the proteins' seconda.y structure in the sa.ples. Pea. positions of the a.ide I ba.ds were determined using the second deriva.ive a.d Fourier self-deconvolution of infra.ed a.d Ra.a. spectra. We obta.ned ba.d a.ea. corresponding to -turn, 3(10)-helix, -helix, -sheet, -turns, a.d side cha.ns for the infra.ed a.d confoca. Ra.a. spectra.by Ga.ssia. fitting. Using the second deriva.ive of the infra.ed a.d Ra.a. spectra. we demonstra.ed the consistency of the vibra.iona. a.signments beca.se compounds of low symmetry without a. inversion center present coincident ba.ds tha. differ only in intensity;hence, the infra.ed procedures to identify a.d a.sign the ba.ds of the proteins' seconda.y structure in the Ra.a. spectra.a.e va.id. The results obta.ned a.e supported by vibra.iona. theory a.d indica.e tha. infra.ed a.d confoca. Ra.a. spectroscopy a.e importa.t, useful, a.d fa.t tools to identify cha.ges in the proteins' seconda.y structure in norma. tissues, goiter, a.d follicula. a.d pa.illa.y thyroid ca.cinoma..

关键词： confoca. Ra.a. spectroscopy goiter infra.ed spectroscopy protein seconda.y structure thyroid ca.cinoma.

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Molecula. a.a.ysis of a.optosis pa.hwa. a.ter photodyna.ic thera.y in brea.t ca.cer: a.ima. model study

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PHOTODIa.NOSIS a.D PHOTODYNa.IC THERa.Y 2016年 14卷 152-158页

作者： Silva. Lucia.a.C. Ferreira.Strixino, Julia.a.Fonta.a. Leticia.C. Rocha.Gonsa.ves, a.tonio M. d'a. Serra. a.menio C. Pineiro, Ma.ta.canevari, renata a. Univ Vale Paraiba UNIVAP IP & D Mol Biol Lab BR-12400000 Sao Jose Dos Campos SP Brazil Univ Vale Paraiba UNIVAP Lab Espect Vibrat Biomed BR-12400000 Sao Jose Dos Campos SP Brazil Univ Vale Paraiba UNIVAP IP & D Lab Terapia Fotodinam BR-12400000 Sao Jose Dos Campos SP Brazil Univ Coimbra Dept Quim Chymiotechnon P-3049535 Coimbra Portugal

Ba.kground: Molecula. investiga.ion of brea.t tumors ha. permitted better understa.ding a.out intera.tion of genes a.d pa.hwa.s involved in tumor progression. Objective: The a.m of this study wa. to eva.ua.e the a.socia.ion between genes belonging to the pa.hwa. of a.optosis with tumor response to photodyna.ic thera.y. Study Design/Ma.eria.s a.d methods: The ma.ma.y tumors were induced in twenty-four Spra.uey-Da.ley fema.e ra.s by ora. ga.a.e of 7,12-dimethylbenz(a.a.thra.ene (8 mg/Kg body weight). a.ima.s were divided into three groups: G1 (norma. tissue), G2 (tumors without trea.ment), G3 (a.ima.s eutha.ized 48 h a.ter trea.ment). The photosensitizer used wa. a.chlorin, 5,15-bis-(2-bromo-5-hydroxyphenyl) chlorin in the dose of 8 mg/kg for ea.h a.ima.. Light source of diode la.er a. a.wa.elength of 660 nm, fluence ra.e of 100 mW/cm, a.d light dose of 100 J/cm wa. delivery to lesions for trea.ment. a.sa.ple from ea.h a.ima. wa. investiga.ed by qua.tita.ive rea. time PCR using Ra. a.optosis RT2 Profiler (TM) PCR a.ra. pla.form. Results: Pro-a.optotic Ba.1, Ca.D6, Ca.P8, CIDEa. CIDEB, Da.K1, TNF, TNFRSF10B, Fa.LG, LOC687813, a.d TP73 genes showed increa.ed expression, a.d CD40 a.ti-a.optotic gene showed decrea.ed expression in the group who underwent PDT (G3) in rela.ion to G2. Conclusion: The results indica.ed tha. these genes a.e involved more directly with cellula. a.optosis induced by PDT using the Chlorin photosensitizer. (C) 2016 Elsevier B.V. a.l rights reserved.

关键词： a.optosis Brea.t ca.cer Photodyna.ic thera.y Chlorin

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Identifica.ion of novel bioma.kers a.socia.ed with poor pa.ient outcomes in inva.ive brea.t ca.cinoma

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TUMOR BIOLOGY 2016年第10期37卷 13855-13870页

作者： canevari, renata a. Ma.chi, Fa.io a. Domingues, Ma.ia.a. C. de a.dra.e, Victor Pia.a.Ca.deira. Jose R. F. Verjovski-a.meida. Sergio Roga.to, Silvia.R. Reis, Edua.do M. Univ Vale Paraiba Inst Pesquisa & Desenvolvimento BR-12244000 Sao Jose Dos Campos SP Brazil CIPE AC Camargo Canc Ctr BR-01508010 Sao Paulo SP Brazil Univ Estado Sao Paulo UNESP Fac Med Dept Patol BR-18618000 Botucatu SP Brazil AC Camargo Canc Ctr Dept Patol BR-01509900 Sao Paulo SP Brazil Hosp Amaral Carvalho Dept Senol BR-17210080 Jau SP Brazil Univ Sao Paulo Inst Quim Dept Bioquim Ave Prof Lineu Prestes748Cidade Univ BR-05508900 Sao Paulo SP Brazil Inst Butantan BR-05503900 Sao Paulo SP Brazil Vejle Sygehus Dept Clin Genet Vejle Denmark Univ Southern Denmark Inst Reg Hlth Vejle Denmark

Brea.t ca.cinoma.(BC) corresponds to 23 % of a.l ca.cers in women, with 1.38 million new ca.es a.d 460,000 dea.hs worldwide a.nua.ly. Despite the significa.t a.va.ces in the identifica.ion of molecula. ma.kers a.d different moda.ities of trea.ment for prima.y BC, the a.ility to predict its meta.ta.ic beha.ior is still limited. The purpose of this study wa. to identify novel molecula. ma.kers a.socia.ed with distinct clinica. outcomes in a.Bra.ilia. cohort of BC pa.ients. We genera.ed globa. gene expression profiles using tumor sa.ples from 24 pa.ients with inva.ive ducta. BC who were followed for a. lea.t 5 yea.s, including a.group of 15 pa.ients with fa.ora.le outcomes a.d a.other with nine pa.ients who developed meta.ta.is. We identified a.set of 58 differentia.ly expressed genes (p a.currency sign 0.01) between the two groups. The prognostic va.ue of this meta.ta.is signa.ure wa. corrobora.ed by its a.ility to stra.ify independent BC pa.ient da.a.ets a.cording to disea.e-free surviva. a.d overa.l surviva.. The upregula.ion of B3GNT7, PPM1D, TNKS2, PHB, a.d GTSE1 in pa.ients with poor outcomes wa. confirmed by qua.tita.ive reverse tra.scription polymera.e cha.n rea.tion (RT-qPCR) in a. independent sa.ple of pa.ients with BC (47 with good outcomes a.d eight tha. presented meta.ta.is). The expression of BCL2-a.socia.ed a.onist of cell dea.h (Ba.) protein wa. determined in 1276 BC tissue sa.ples by immunohistochemistry a.d wa. consistent with the reduced Ba. mRNa.expression levels in meta.ta.ic ca.es, a. observed in the oligoa.ra. da.a. These findings point to novel prognostic ma.kers tha. ca. distinguish brea.t ca.cinoma. with meta.ta.ic potentia. from those with fa.ora.le outcomes.

关键词： Brea.t ca.cer Prognostic gene signa.ure Meta.ta.is Gene expression Tumor bioma.kers

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a.plica.ions of Ra.a. Spectroscopy in Life Science

Applications of Raman Spectroscopy in Life Science

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Joint Meeting of the 1st SPIE Conference on Biophotonics South a.erica.(BSa. / 15th World Congress of the Interna.iona.-Photodyna.ic-a.socia.ion (IPa.

作者： Ma.tin, a.rton a. Soto, Cla.dio a. T. a.i, Syed M. Neto, La.a.o P. M. canevari, renata a. Pereira. Lilia.e Fa.ero, Priscila.P. Univ Vale Paraiba UniVap Inst Res & Dev IP&D Lab Biomed Vibrat Spect BR-12244400 Sao Jose Dos Campos SP Brazil

ISBN: (纸本)9781628416961

Ra.a. spectroscopy ha. been a.plied to the a.a.ysis of biologica. sa.ples for the la.t 12 yea.s providing detection of cha.ges occurring a. the molecula. level during the pa.hologica. tra.sforma.ion of the tissue. The potentia. use of this technology in ca.cer dia.nosis ha. shown encoura.ing results for the in vivo, rea.-time a.d minima.ly inva.ive dia.nosis. Confoca. Ra.a. technics ha. a.so been successfully a.plied in the a.a.ysis of skin a.ing process providing new insights in this field. In this pa.er it is presented the la.est biomedica. a.plica.ions of Ra.a. spectroscopy in our la.ora.ory. It is shown tha. Ra.a. spectroscopy (RS) ha. been used for biochemica. a.d molecula. cha.a.teriza.ion of thyroid tissue by micro-Ra.a. spectroscopy a.d gene expression a.a.ysis. This study a.med to improve the discrimina.ion between different thyroid pa.hologies by Ra.a. a.a.ysis. a.tota. of 35 thyroid tissues sa.ples including norma. tissue (n=10), goiter (n=10), pa.illa.y (n=10) a.d follicula. ca.cinoma. (n=5) were a.a.yzed. The confoca. Ra.a. spectroscopy a.lowed a.ma.imum discrimina.ion of 91.1% between norma. a.d tumor tissues, 84.8% between benign a.d ma.igna.t pa.hologies a.d 84.6% a.ong ca.cinoma. a.a.yzed. It will be a.so report the a.plica.ion of in vivo confoca. Ra.a. spectroscopy a. a. importa.t sensor for detecting a.va.ced glyca.ion products (a.Es) on huma. skin.

关键词： Ra.a. spectroscopy Thyroid ca.cer in vivo confoca. Ra.a. huma. skin glyca.ion a.Es

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Biochemica. a.d molecula. cha.a.teriza.ion of thyroid tissue by micro-Ra.a. spectroscopy a.d gene expression a.a.ysis 12

Biochemical and molecular characterization of thyroid tissue...

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Conference on Photonic Thera.eutics a.d Dia.nostics XII

作者： Neto, La.a.o P. M. Ma.tin, a.rton a. Soto, Cla.dio a. T. Sa.tos, a.dre B. Mello, Eva.dro S. Pereira. Ma.ina.a. Cernea. Cla.dio R. Bra.da., Lenine G. canevari, renata a. Univ Vale Paraiba UNIVAP Inst Pesquisa & Desenvolvimento Ave Shishima Hifumi 2911 BR-12244000 Urbanova Sao Jose Dos Ca Brazil Univ Sao Paulo Fac Med Hosp Clin Ave Doutor Arnaldo 455 BR-01246903 Sao Paulo Brazil

ISBN: (纸本)9781628419245

Thyroid ca.cinoma. represent the ma.n endocrine ma.igna.cy a.d their dia.nosis ma. produce inconclusive results. Ra.a. spectroscopy a.d gene expression a.a.ysis ha.e shown excellent results on the differentia.ion of ca.cinoma.. This study a.med to improve the discrimina.ion between different thyroid pa.hologies combining of both a.a.yses. a.tota. of 35 thyroid tissues sa.ples including norma. tissue (n= 10), goiter (n= 10), pa.illa.y (n= 10) a.d follicula. ca.cinoma. (n= 5) were a.a.yzed. Confoca. Ra.a. spectra.wa. obta.n by using a.Rivers Dia.nostic System, 785 nm la.er excita.ion a.d CCD detector. The da.a.wa. processed by the softwa.e La.spec5 a.d Origin 8.5 a.d a.a.yzed by Minita. (R) progra.. The gene expression a.a.ysis wa. performed by qRT-PCR technique for TG, TPO, PDGFB, SERPINa., LGa.S3 a.d TFF3 genes a.d sta.istica.ly a.a.yzed by Ma.n-Whitney test. The confoca. Ra.a. spectroscopy a.lowed a.ma.imum discrimina.ion of 91.1% between norma. a.d tumor tissues, 84.8% between benign a.d ma.igna.t pa.hologies a.d 84.6% a.ong ca.cinoma. a.a.yzed. Significa.t differences wa. observed for TG, LGa.S3, SERPINa. a.d TFF3 genes between benign lesions a.d ca.cinoma., a.d SERPINa. a.d TFF3 genes between pa.illa.y a.d follicula. ca.cinoma.. Principa. component a.a.ysis wa. performed using PC1 a.d PC2 in the pa.illa.y ca.cinoma.sa.ples tha. showed over gene expression when compa.ed with norma. sa.ple, where 90% of discrimina.ion wa. observed a. the a.ide 1 (1655 cm(-1)), a.d a. the tyrosine spectra.region (856 cm(-1)). The discrimina.ion of tissues thyroid ca.ried out by confoca. Ra.a. spectroscopy a.d gene expression a.a.ysis indica.e tha. these techniques a.e promising tools to be used in the dia.nosis of thyroid lesions.

关键词： Ra.a. spectroscopy a.a.ysis Gene expression Molecula. ma.kers Thyroid ca.cinoma. Thyroid Dia.nostic

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The role of proteoglyca. genes in PDT-resista.t HEp-2 cells 17

The role of proteoglycan genes in PDT-resistant HEp-2 cells

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17th World Congress of the Interna.iona.-Photodyna.ic-a.socia.ion (IPa.

作者： Pa.heco-Soa.es, Cristina.de Fa.ia.Souza. Roberta.Kelly Silva.Ca.va.ho, Isa.el Cha.es canevari, renata a. Godoi, Bruno Henrique Mora.s, Ca.los Da.lton G. O. Ricci, Ritchelli Strixino, Julia.a.Ferreira Univ Vale Paraiba Inst Res & Dev 7 Lab Dynam Cellular Compartments Av Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil Univ 13 Vale Paraiba Inst Res & Dev Lab Mol Biol Av Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos 14 SP Brazil Univ 13 Vale Paraiba Inst Res & Dev Lab Photodynam Therapy Av Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos 14 SP Brazil

ISBN: (数字)9781510628342

ISBN: (纸本)9781510628342

Optimizing the pa.a.eters of PDT for specific ca.cer cells by identifying the mecha.isms involved in the process represents a.considera.le tool for the development of photosensitizer delivery systems a.d the improvement of PDT ta.gets for ca.cer. Proteoglyca.s, importa.t constituents of extra.ellula. ma.rix (ECM) pla. a. importa.t role in both progression a.d inhibition of tumor development. This study determines by gene expression whether proteoglyca. a.e a.socia.ed with PDT resista.ce observed in huma. epidermoid la.ynx ca.cinoma.cells (HEp-2). We conclude tha. PDT increa.es the expression of DECORIN a.d BIGLYCa. genes, involved in meta.ta.ic dissemina.ion, lea.ing to inhibitory effect growth in Hep-2 cells

关键词： Photodyna.ic thera.y Ca.cer Resista.ce Tumors Oncology Photosensitizer ta.geting

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Na.ohydroxya.a.ite/Gra.hene Na.oribbons Na.ocomposites Induce in Vitro Osteogenesis a.d Promote in Vivo Bone Neoforma.ion

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a.S BIOMa.ERIa.S SCIENCE & ENGINEERING 2018年第5期4卷 1580-1590页

作者： Medeiros, Joelson S. Oliveira. a.relia.o M. de Ca.va.ho, Ja.cineide O. Ricci, Ritchelli Ma.tins, Ma.ia.do C. C. Rodrigues, Bruno V. M. Webster, Thoma. J. Via.a. Ba.tolomeu C. Va.concellos, Lua.a.M. R. canevari, renata a. Ma.cia.o, Ferna.da.R. Lobo, a.derson O. Univ Brasil Inst Ciencia & Tecnol Rua Carolina da Fonseca 584 BR-08230030 Bairro Itaquera SP Brazil Ctr Univ Uninovafapi Rua Vitorino Orthiges Fernandes 6123 BR-64073505 Teresina Piaui Brazil Univ Vale Paraiba Lab Biol Mol Canc Av Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil Univ Fed Piaui Ctr Ciencias Nat Dept Biofis & Fisiol CCS Campus Univ Ministro Petronio Portella BR-64049550 Teresina Piaui Brazil Univ Fed Piaui Ctr Ciencias Nat Lab Interdisciplinar Mat Avancados Programa Posgrad Ciencia & Engn Mat Campus Univ Ministro Petronio Portella BR-64049550 Teresina Piaui Brazil Univ Fed Piaui Ctr Ciencias Nat Dept Fis Campus Univ Ministro Petronio Portella BR-64049550 Teresina Piaui Brazil Northeastern Univ Dept Chem Engn 360 Huntington Ave Boston MA 02115 USA Univ Estadual Sao Paulo Inst Ciencia & Tecnol Dept Biociencias & Diagnost Oral Ave Eng Francisco Jose Longo 777 BR-12245000 Sao Jose Dos Campos SP Brazil MIT Dept Chem 77 Massachusetts Ave18-393 Cambridge MA 02139 USA

Na.oma.eria.s ba.ed on gra.hene oxide na.oribbons (GNR) a.d na.ohydroxya.a.ite (nHa.) serve a. a.tra.tive ma.eria.s for bone tissue engineering. Herein, we eva.ua.ed the potentia. of nHa./GNR towa.d in vitro a.a.ysis of specific genes rela.ed to osteogenesis a.d in vivo bone regenera.ion using a.ima. model. Three different concentra.ions of nHa./GNR composites were a.a.yzed in vitro using a.cytotoxicity a.sa., a.d osteogenic potentia. wa. determined by a.P, OPN, OCN, COL1, a.d RUNX2 genes a.d a.ka.ine phospha.a.e a.sa.s. In vivo bone neoforma.ion using a.well-esta.lished in vivo ra. tibia.defect model wa. used to confirm the efficiency of the optimized composite. The sca.folds were nontoxic, a.d the osteogenesis process wa. dose-dependent (a. 200 mu g mL(-1) of nHa./GNR) compa.ed to controls. The in vivo results showed higher bone neoforma.ion a.ter 15 da.s of nHa./GNR impla.ta.ion compa.ed to a.l groups. a.ter 21 da.s, both nHa./GNR composites showed better la.ella. bone forma.ion compa.ed to control. We a.tributed this enha.ced bone neoforma.ion to the high bioa.tivity a.d surfa.e a.ea.presented by nHa./GNR composites, which wa. systema.ica.ly eva.ua.ed in previous studies. These new in vivo results suggest tha. nHa./GNR composites ca. be exploited for a.ra.ge of stra.egies for the improved development of novel denta. a.d orthopedic bone gra.ts to a.celera.e bone regenera.ion.

关键词： gra.hene oxide na.oribbons na.ohydroxya.a.ite in vivo bone neoforma.ion gene expression

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