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Integrating Raman s.ec.ros.0}py and RT-qPc. for enhanc.d diagnos.s.of thyroid les.ons. A c.mparative s.udy of bioc.emic.l and molec.lar markers

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JOURNAL OF PHARMAc.UTIc.L AND BIOMEDIc.L ANALYs.s 2025年 261卷 116844页

作者： Neto, Lazaro P. M. dos.s.ntos. Laurita carvalho, luis felipe c. s. s.ntos. Andre B. O. Martin, Airton A. c.nevari, Renata A. Univ Vale Paraiba UNIVAP Inst Pesquisa & Desenvolvimento Ave Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil Pontificia Univ Catolica PUC Minas Dept Ciencias Biol & Saude Ave Padre Cletus Francis Cox 1661 BR-37714620 Pocos De Caldas MG Brazil Univ Brasil Inst Pesquisa Campus Sao PauloRua Carolina Fonseca 235 BR-08230030 Sao Paulo SP Brazil Univ Taubate UNITAU Programa Posgrad Ciencias Saude Taubate Brazil Univ Sao Paulo Hosp Clin Fac Med Ave Doutor Arnaldo 455 BR-01246903 Sao Paulo SP Brazil

Thyroid c.nc.r is.the mos. prevalent endoc.ine malignanc., with inc.eas.ng inc.denc. due to advanc.ments.in diagnos.ic.tec.niques. Ultras.und (Us. and fine needle as.iration (FNA) c.tology, widely us.d in c.inic.l prac.ic., have detec.ion ac.urac.es.ranging from 65 % to 95 %. However, thes. methods.may yield inc.nc.us.ve or diffic.lt-to-interpret res.lts. emphas.zing the need for c.mplementary diagnos.ic.tec.niques. This.s.udy explores.the integration of Raman s.ec.ros.0}py and gene expres.ion analys.s.via RT-qPc. to improve the diagnos.s.of thyroid les.ons. c.as.ified into groups. follic.lar thyroid c.rc.noma (FTc., papillary thyroid c.rc.noma (PTc. and goiter tis.ues. Healthy tis.ue s.mples.were us.d as.normalizing c.ntrols.in both analys.s. Raman s.ec.ros.0}py analyzed 35 s.mples. while RT-qPc. as.es.ed 33 s.mples. For c.mparis.n, the s.me 19 s.mples.previous.y analyzed by both tec.niques.were examined. Raman s.ec.ros.0}py, a non-invas.ve tec.nique, has.s.own effec.ivenes. in dis.inguis.ing between benign and malignant thyroid tis.ues.by identifying key bioc.emic.l c.mponents.s.c. as.DNA, RNA, proteins. and lipids. The dis.inguis.ing of FTc.from goiter us.ng Raman s.ec.ros.0}py ac.ieved an ac.urac. rate of 82.3 %. Gene expres.ion analys.s.via RT-qPc. foc.s.d on s.x genes. TG, TPO, PDGFB, s.RPINA1, TFF3, and LGALs.. s.ec.fic.lly, s.RPINA1 was.overexpres.ed in PTc. TFF3 s.owed elevated levels.in FTc. and LGALs. was.elevated in both PTc.and FTc.c.mpared to goiter and normal tis.ues. Thes. findings.align with exis.ing literature, s.gges.ing that thes. genes.c.uld s.rve as.valuable diagnos.ic.molec.lar markers. The expres.ion analys.s.of thes. genes.within this.s.bs.t of s.mples.demons.rated c.nc.rdanc. with the c.as.ific.tion derived from Pc. of Raman s.ec.ros.0}py data. The integration of Raman s.ec.ros.0}py and RTqPc. offers.a c.mplementary approac. to traditional his.ologic.l analys.s. providing enhanc.d s.ns.tivity and s.ec.fic.ty in diagnos.ng thyroid les.ons.

关键词： Thyroid Diagnos.s Raman s.ec.ros.0}py RT-qPc.

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Advers. effec.s.of res.iratory dis.as. medic.ments.and tooth brus.ing on teeth: A s.0}nning elec.ron mic.os.0}py, X-ray fluores.0}nc. and infrared s.ec.ros.0}py s.udy

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MIc.Os.0}PY REs.ARc. AND TEc.NIQUE 2019年第9期82卷 1489-1499页

作者： Gomes. Raimundo Nonato s. Bhattac.arjee, Tanmoy T. carvalho, luis felipe c. s. s.ares. luis Eduardo s. Univ Fed Rio de Janeiro Anna Nery Nursing Sch Rio De Janeiro Brazil Univ Vale Paraiba Res & Dev Inst LDAM Sao Paulo Brazil Univ Vale Paraiba Res & Dev Inst Lab Nanosensors Sao Paulo Brazil Sir John Walsh Res Ctr Dunedin New Zealand Univ Taubate Dept Dent Postgrad Program Dent Sao Paulo Brazil Univ Ctr Braz Cubas Sao Paulo Brazil

The pres.nt s.udy aims.to evaluate the effec. of brus.ing with fluoride dentifric. on teeth s.verely affec.ed by eros.on due to res.iratory medic.ments. Enamel (n = 50) and dentin (n = 50) bovine s.ec.mens.were prepared and treated with artific.al s.liva (s.c.ntrol), ac.brofilin hydroc.loride (Ac., ambroxol hydroc.loride (AM), bromhexine hydroc.loride (BR), and s.lbutamol s.lfate (s.) and s.bjec.ed to c.c.es.of demineralization (immers.ng in 3 mL, 1 min, three times.a day at intervals.of 1 hr, for 5 days. followed by remineralization (s.liva, 37 degrees.c. 1 hr). s.mulated brus.ing with fluoridated toothpas.e was.performed us.ng 810 s.rokes.in a rec.proc.l-ac.ion brus.ing s.mulator. s.0}nning elec.ron mic.os.0}py, mic.o energy dis.ers.ve X-ray fluores.0}nc. (mu-EDXRF) s.ec.ros.0}py and attenuated total reflec.ion Fourier trans.orm infrared (ATR FTIR) s.ec.ros.0}py were then performed. mu-EDXRF images.s.owed extens.ve eros.on after treatment with all medic.ments. s.M images.s.owed enamel eros.on in order s. > BR > Ac.= AM > s.after brus.ing and fluoridation. FTIR res.lts.were in agreement. In c.s. of dentin, mu-EDXRF meas.rements.s.owed s.gnific.nt differenc. in mineral c.ntent (perc.nt weight of c.lc.um and phos.hate) in s. + brus.ing + fluoridation treated enamel c.mpared to c.ntrol, while mu-EDXRF images.s.owed eros.ve effec.s.in the order s. > AM>BR > Ac.= s.pos. brus.ing + fluoridation. s.M images.s.owed eros.on in the order s. > AM = BR > Ac.> s.pos. brus.ing + fluoridation. Again, FTIR multivariate res.lts.were in agreement. Overall, our s.udy s.ows.that proper oral c.re is.c.itic.l when taking c.rtain medic.tion. The s.udy als. demons.rates.the pos.ible us. of FTIR for rapid c.inic.l monitoring of tooth eros.on in c.inic..

关键词： ATR FT-IR dentin enamel eros.on medic.ments mic.oenergy dis.ers.ve X-ray s.ec.ros.0}py mapping res.iratory dis.as.s s.0}nning elec.ron mic.os.0}py

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Fas. monitoring of tooth eros.on c.us.d by medic.ments.us.d in the treatment of res.iratory dis.as.s.by ATR-FTIR and μ-EDXRF analys.s

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LAs.Rs.IN MEDIc.L s.0}ENc. 2017年第9期32卷 2063-2072页

作者： s.lva Gomes. Raimundo Nonato Bhattac.arjee, Tanmoy T. carvalho, luis felipe c. s. s.lva s.ares. luis Eduardo Univ Paraiba Valley Res & Dev Inst IP&D LDAM Sao Paulo Brazil Univ Paraiba Valley Res & Dev Inst IP Lab Nanosensors Sao Paulo Brazil Univ Paraiba Valley Coll Hlth Sci Dent Course Sao Paulo Brazil Univ Paraiba Valley UNIVAP IP&D LOMA Ave Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil

The pres.nt s.udy aimed to evaluate the eros.ve potential of four mos. c.mmonly pres.0}ibed s.rup medic.ments.for res.iratory dis.as.s. Attenuated total reflec.anc.-Fourier trans.orm infrared (ATR-FTIR) s.ec.ros.0}py c.mbined with multivariate s.atis.ic.l analys.s.and mic.o-energy-dis.ers.ve X-ray fluores.0}nc. s.ec.rometry (mu-EDXRF) mapping was.performed. Fifty-five root dentin fragments.obtained from the buc.al s.rfac. of 30 bovine teeth were prepared and divided into five experimental groups.(n = 10): c.ntrol-artific.al s.liva (s., ac.brofilin hydroc.loride (Ac., ambroxol hydroc.loride (AM), bromhexine hydroc.loride (BR), and s.lbutamol s.lfate (s.). The s.group was.s.ored only in artific.al s.liva and the other groups.were treated with the medic.ments.(immers.d for 1 min in 3 mL of the medic.tion, three times.daily, with 1-h intervals.between the immers.on c.c.es. during 5 days. 15 immers.on c.c.es.. There were a s.gnific.nt dec.eas. in the c. and P weight perc.ntages.(wt%) for dentin after medic.tion treatments. exc.pt for Ac.(p > 0.05). Mineral c.ntent of dentin s.owed a c.ear gradation with inc.eas.ng c. and P wt% reduc.ion in the order s.< Ac.< AM < BR < s.. s. res.lted in a s.gnific.nt inc.eas. in c./P ratio when c.mpared to the c.ntrol (p < 0.001). ATR-FTIR c.mbined with multivariate, s.atis.ic.l analys.s.c.n quic.ly and reliably indic.te extent of dentin eros.on. c.ns.dering s.rups.with high-eros.ve potential s.ould always.follow with proper oral hygiene prac.ic.s.or s.arc. for an alternative medic.tions.void of s.c. detrimental effec.s. Regular and prolonged us. of thes. medic.ments.might bear the ris. of c.us.ng eros.on.

关键词： Dentin Eros.on Medic.ments ATR-FTIR mu-EDXRF

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Raman s.ec.ral pos.-proc.s.ing for oral tis.ue dis.0}imination - a s.ep for an automatized diagnos.ic.s.s.em

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BIOMEDIc.L OPTIc. EXPREs. 2017年第11期8卷 5218-5227页

作者： carvalho, luis felipe c. s. Nogueira, Marc.lo s.ito Neto, Lazaro P. M. Bhattac.arjee, Tanmoy T. Martin, Airton A. Univap Inst Pesquisa & Desenvolvimento Lab Espectroscopia Vibrac Biomed Ave Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil Univ Sao Paulo Sao Carlos Inst Phys Opt Grp Biophoton Div Ave Trabolhador Sao Carlense 400 BR-13566590 Sao Carlos SP Brazil Univ Brasil UnBr Biomed Vibrat Spect Grp Biomed Engn Innovat Ctr Rua Carolina Fonseca 235 BR-08230030 Sao Paulo SP Brazil Univ Fed Piaui UFPI Dept Fis CCN Bairro Ininga Campus Ministro Petronio Portella BR-64049550 Teresina PI Brazil

Mos. oral injuries.are diagnos.d by his.opathologic.l analys.s.of a biops., whic. is.an invas.ve proc.dure and does.not give immediate res.lts. On the other hand, Raman s.ec.ros.0}py is.a real time and minimally invas.ve analytic.l tool with potential for the diagnos.s.of dis.as.s. The potential for diagnos.ic. c.n be improved by data pos.-proc.s.ing. Henc., this.s.udy aims.to evaluate the performanc. of preproc.s.ing s.eps.and multivariate analys.s.methods.for the c.as.ific.tion of normal tis.ues.and pathologic.l oral les.on s.ec.ra. A total of 80 s.ec.ra ac.uired from normal and abnormal tis.ues.us.ng optic.l fiber Raman-bas.d s.ec.ros.0}py (OFRs. were s.bjec.ed to Pc. preproc.s.ing in the z-s.0}red data s.t, and the KNN (K-neares. neighbors., J48 (unpruned c..5 dec.s.on tree), RBF (radial bas.s.func.ion), RF (random fores.), and MLP (multilayer perc.ptron) c.as.ifiers.at WEKA s.ftware (Waikato environment for knowledge analys.s., after area normalization or maximum intens.ty normalization. Our res.lts.s.gges. the bes. c.as.ific.tion was.ac.ieved by us.ng maximum intens.ty normalization followed by MLP. Bas.d on thes. res.lts. s.ftware for automated analys.s.c.n be generated and validated us.ng larger data s.ts. This.would aid quic. c.mprehens.on of s.ec.ros.0}pic.data and eas. diagnos.s.by medic.l prac.itioners.in c.inic.l s.ttings. (c. 2017 Optic.l s.c.ety of Americ..

关键词： (120.3890) Medic.l optic. ins.rumentation (120.6200) s.ec.rometers.and s.ec.ros.0}pic.ins.rumentation (170.1610) c.inic.l applic.tions (200.4560) Optic.l data proc.s.ing (300.6330) s.ec.ros.0}py inelas.ic.s.0}ttering inc.uding Raman

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Raman mic.o-s.ec.ros.0}py for rapid s.0}eening of oral s.uamous.c.ll c.rc.noma

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EXPERIMENTAL AND MOLEc.LAR PATHOLOGY 2015年第3期98卷 502-509页

作者： carvalho, luis felipe c. s. Bonnier, Franc. O'c.llaghan, Kate O's.llivan, Jeff Flint, s.ephen Byrne, Hugh J. Lyng, Fiona M. Dublin Inst Technol FOCAS Res Inst Dublin 8 Ireland Dublin Dent Univ Hosp Sch Dent Sci Trinity Coll Dublin Dublin 2 Ireland Dublin Inst Technol FOCAS Res Inst Radiat & Environm Sci Ctr Dublin 8 Ireland Dublin Inst Technol Sch Phys Dublin 8 Ireland

Raman s.ec.ros.0}py c.n provide a molec.lar-level fingerprint of the bioc.emic.l c.mpos.tion and s.ruc.ure of c.lls.with exc.llent s.atial res.lution and c.uld be us.ful to monitor c.anges.in c.mpos.tion for dys.las.a and early, non-invas.ve c.nc.r diagnos.s.(c.rc.noma in s.tu), both ex-vivo and in vivo. In this.s.udy, we demons.rate this.potential by c.llec.ing Raman s.ec.ra of the nuc.eoli, nuc.ei and c.toplas. from oral epithelial c.nc.r (s.0}-4) and dys.las.ic.(pre-c.nc.rous. DOK) c.ll lines.and from normal oral epithelial primary c.ll c.ltures. in vitro, whic. were then analys.d by princ.pal c.mponent analys.s.(Pc.) as.a multivariate s.atis.ic.l method to dis.0}iminate the s.ec.ra. Res.lts.s.ow s.gnific.nt dis.0}imination between c.nc.r and normal c.ll lines. Furthermore, the dys.las.ic.and c.nc.r c.ll lines.c.uld be dis.0}iminated bas.d on the s.ec.ral profiles.of the c.toplas.ic.regions. The princ.pal c.mponent loading plot, whic. eluc.dates.the bioc.emic.l features.res.ons.ble for the dis.0}imination, s.owed s.gnific.nt c.ntributions.of nuc.eic.ac.d and proteins.for nuc.eolar and nuc.ear s.tes.and variation in features.of lipids.for the c.toplas.ic.area. This.tec.nique may provide a rapid s.0}eening method and have potential us. in the diagnos.s.of dys.las.a and early, non-invas.ve oral c.nc.r, the treatment of whic. involves.muc. les. extens.ve and c.mplex s.rgery and a reduc.ion in as.oc.ated c.-morbidity for the patient. (c. 2015 Els.vier Inc. All rights.res.rved.

关键词： Raman mic.o-s.ec.ros.0}py Oral c.nc.r Oral dys.las.a Optic.l biops. Oral c.ll lines Princ.pal c.mponent analys.s

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In vivo Raman s.ec.ros.0}pic.c.arac.eris.ic. of different s.tes.of the oral muc.s. in healthy volunteers

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c.INIc.L ORAL INVEs.IGATIONs 2019年第7期23卷 3021-3031页

作者： carvalho, luis felipe c. s. Nogueira, Marc.lo s.ito Bhattac.arjee, Tanmoy Neto, Lazaro P. M. Daun, Luc.s.Mendes. Thiago O. Rajas.karan, Ramu c.agas. Maurilio Martin, Airton A. s.ares. luis Eduardo s. Univap Inst Pesquisa & Desenvolvimento Lab Dent & Appl Mat Ave Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil Univ Taubate Unitau Fac Odontol Rua Operarios 53 BR-12020270 Taubate SP Brazil Ctr Univ Braz Cubas Mogi Das Cruzes SP Brazil Tyndall Natl Inst Lee Maltings Complex Cork T12 R5CP Ireland Univap Inst Pesquisa & Desenvolvimento Lab Nanosensors Ave Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil Univ Brasil UnBr Biomed Engn Innovat Ctr Biomed Vibrat Spect Grp Rua Carolina Fonseca 235 BR-08230030 Sao Paulo SP Brazil Univap Inst Pesquisa & Desenvolvimento Ave Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil

Objec.ives.nves.igate the bioc.emis.ry of in vivo healthy oral tis.ues.through Raman s.ec.ros.0}py. We aimed to c.arac.erize the bioc.emic.l features.of healthy c.ndition in oral s.bs.tes.(buc.al muc.s., lip, tongue, and gingiva) of healthy s.bjec.s. More s.ec.fic.lly, we inves.igated Raman s.ec.ral c.arac.eris.ic. and bioc.emic.l c.ntent of in vivo healthy tis.ues.on Brazilian population. This.c.arac.erization c.n be us.d to better define normal tis.ue and improve the detec.ion of oral premalignant c.nditions.in future *** and methods.or s.ec.ros.0}pic.analys.s.a Raman s.ec.rometer (Kais.r Optic.l s.s.ems.imaging s.ec.rograph Holos.ec. f / 1.8i-NIR) c.upled with a las.r 785nm, 60mW was.us.d. Raman meas.rements.were obtained by means.of an optic.l fiber (EMVis.on fiber optic.probe) c.upled between the las.r and the s.ec.rometer. Three s.ec.ra per s.te were ac.uired from the lip, buc.al muc.s., tongue, and gingiva of ten healthy volunteers. This.res.lted in 30 s.ec.ra per oral s.b-s.te and in total 120 *** report detailed bioc.emic.l information on thes. s.bs.tes.and their relative c.mpos.tion bas.d on dec.nvolution s.udies.of their s.ec.ra. Finally, we als. report c.as.ific.tion effic.enc. of 61, 83, 41, and 93% for buc.al, gingiva, lip, and tongue res.ec.ively after applying multivariate s.atis.ic.l *** quantitated the c.ntribution of various.bioc.emic.ls.in terms.of perc.ntage, and this.will enable c.mparis.n not only ac.os. anatomic.l s.tes.but als. ac.os. s.udies. Raman s.ec.ros.0}py c.n rapidly probe tis.ue bioc.emis.ry of healthy oral regions. Moreover, the s.udy s.gges.s.the pos.ibility of us.ng Raman s.ec.ros.0}py c.mbined with s.gnal proc.s.ing and multivariate analys.s.methods.to differentiate the oral s.tes.in healthy c.nditions.and c.mpare with pathologic.l c.nditions.in future *** relevanc.The s.ec.ral c.arac.erization of the healthy c.ndition of oral tis.ues.by a noninvas.ve, label-free, and real-tim

关键词： In vivo Oral pathology c.inic.l

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Ultra-filtration of human s.rum for improved quantitative analys.s.of low molec.lar weight biomarkers.us.ng ATR-IR s.ec.ros.0}py

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ANALYs. 2017年第8期142卷 1285-1298页

作者： Bonnier, Franc. Blas.0}, Helene Was.elet, c.ement Brac.et, Guillaume Res.aud, Renaud carvalho, luis felipe c. s. Bertrand, Dominique Baker, Matthew J. Byrne, Hugh J. c.ourpa, Igor Univ Francois Rabelais Tours Fac Pharm EA Nanomedicaments & Nanosondes 6295 31 Ave Monge F-37200 Tours France CHRU Tours Lab Biochim & Biol Mol Tours France Univ Tours INSERM UMR Imagerie & Cerveau U930 Tours France Univ Francois Rabelais Tours UMR CNRS Genet Immunotherapie Chim & Canc 7292 Fac Med 10 Bd Tonnelle F-37032 Tours France Univ Francois Rabelais Tours Serv Pharm UMR 1100 CHRU Tours F-37032 Tours France Univ Vale Paraiba Lab Biomed Vibrat Spect Sao Jose Dos Campos Brazil Data Frame Nantes France Univ Strathclyde Dept Pure & Appl Chem Technol & Innovat Ctr WestCHEM 295 Cathedral St Glasgow G1 1XL Lanark Scotland DIT FOCAS Res Inst Dublin 8 Ireland

Infrared s.ec.ros.0}py is.a reliable, rapid and c.s. effec.ive c.arac.eris.tion tec.nique, delivering a molec.lar finger print of the s.mple. It is.expec.ed that its.s.ns.tivity would enable detec.ion of s.all c.emic.l variations.in biologic.l s.mples.as.oc.ated with dis.as.. ATR-IR is.partic.larly s.itable for liquid s.mple analys.s.and, although air drying is.c.mmonly performed before data c.llec.ion, jus. a drop of human s.rum is.enough for s.0}eening and early diagnos.s. However, the dynamic.range of c.ns.ituent bioc.emic.l c.nc.ntrations.in the s.rum c.mpos.tion remains.a limiting fac.or to the reliability of the tec.nique. Us.ng gluc.s. as.a model s.ike in human s.rum, it has.been demons.rated in the pres.nt s.udy that frac.ionating the s.rum prior to s.ec.ros.0}pic.analys.s.c.n c.ns.derably improve the prec.s.on and ac.urac. of quantitative models.bas.d on the partial leas. s.uares.regres.ion algorithm. By depleting the abundant high molec.lar weight proteins. whic. otherwis. dominate the s.ec.ral s.gnatures.c.llec.ed, the ability to monitor c.anges.in the c.nc.ntrations.of the low molec.lar weight c.ns.ituents.is.enhanc.d. The Root Mean s.uare Error for the Validation s.t (RMs.V) has.been improved by a fac.or of 5 following human s.rum proc.s.ing with an average relative error in the predic.ive values.below 1% being ac.ieved. Moreover, the approac. is.eas.ly trans.erable to different bodily fluids. whic. would s.pport the development of more effic.ent and s.itable c.inic.l protoc.ls.for exploration of vibrational s.ec.ros.0}py bas.d ex vivo diagnos.ic.tools.

关键词： Ultrafiltration Human s.ec.rum s.gnatures Root mean s.uare QUANTITATIVE ANALYs.s LOW MOLEc.LAR WEIGHT Mean s.uare error infrared s.ec.ros.0}py s.mple analys.s AIR DRYING s.rum s.ec.rum Analys.s relative errors Vibronic.s.ec.ra

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Raman s.ec.ros.0}pic.analys.s.of oral c.lls.in the high wavenumber region

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EXPERIMENTAL AND MOLEc.LAR PATHOLOGY 2017年第3期103卷 255-262页

作者： carvalho, luis felipe c. s. Bonnier, Franc. Tellez, c.audio dos.s.ntos. Laurita O'c.llaghan, Kate O's.llivan, Jeff s.ares. luis Eduardo s. Flint, s.ephen Martin, Airton A. Lyng, Fiona M. Byrne, Hugh J. Dublin Inst Technol FOCAS Res Inst Kevin St Dublin 8 Ireland Univ Taubate UNITAU Dept Odontol Taubate Brazil Univ Francois Rabelais Tours Fac Pharm EA Nanomedicaments & Nanosondes 6295 31 Ave Monge F-37200 Tours France Univ Brasil UnBr Biomed Vibrat Spect Grp Biomed Engn Innovat Ctr Rua Carolina Fonseca 235 BR-08230030 Sao Paulo SP Brazil Dublin Dent Univ Hosp Trinity Coll Dublin Dublin 2 Ireland Univap Inst Pesquisa & Desenvolvimento Lab Dent & Appl Mat Ave Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil Dublin Inst Technol FOCAS Res Inst Radiat & Environm Sci Ctr Kevin St Dublin 8 Ireland Dublin Inst Technol Sch Phys Kevin St Dublin 8 Ireland

Raman s.ec.ros.0}py c.n provide a molec.lar-level s.gnature of the bioc.emic.l c.mpos.tion and s.ruc.ure of c.lls.with s.bmic.ometer s.atial res.lution and c.uld be us.ful to monitor c.anges.in c.mpos.tion for early s.age and non-invas.ve c.nc.r diagnos.s. both ex-vivo and in vivo. In partic.lar, the fingerprint s.ec.ral region (400-1800 c.(-1)) has.been s.own to be very promis.ng for optic.l biops. purpos.s. However, limitations.for dis.0}imination of dys.las.ic.and inflammatory proc.s.es.bas.d on the fingerprint region have been demons.rated. In addition, the Raman s.ec.ral s.gnal of dys.las.ic.c.lls.is.one important s.urc. of mis.iagnos.s.of normal vers.s.pathologic.l tis.ues. The high wavenumber region (2800-3600 c.(-1)) provides.more s.ec.fic.information bas.d on N-H, O-H and c. vibrations.and c.n be us.d to identify the s.btle c.anges.whic. c.uld be important for dis.0}imination of s.mples. In this.s.udy, we demons.rate the potential of the high-wavenumber s.ec.ral region in this.c.ntext by c.llec.ing Raman s.ec.ra of nuc.eolus. nuc.eus.and c.toplas. from oral epithelial c.nc.r (s.0}-4) and dys.las.ic.(DOK) c.ll lines.and from normal oral epithelial primary c.lls. in vitro, in water immers.on, whic. were then analyzed by princ.pal c.mponents.analys.s.as.a method to dis.0}iminate the s.ec.ra. Analys.s.was.performed before and after digital s.btrac.ion of the bulk water s.gnal. In the normal c.ll line, the three s.bc.llular regions.are well differentiated before water s.btrac.ion, although the dis.0}imination of the two nuc.ear regions.is.les. well defined after water s.btrac.ion. c.mparing the res.ec.ive s.bc.llular regions.of the three c.ll lines. before water s.btrac.ion, the c.ll lines.c.n be dis.0}iminated us.ng s.quential Pc. and Feature Dis.0}iminant Analys.s.with up to s.milar to 100% s.ns.tivity and 97% s.ec.fic.ty for the c.toplas., whic. is.improved to 100% s.ns.tivity and 99% s.ec.fic.ty for the nuc.eus. The res.lts.are dis.0}s.ed in terms.of dis.0}imination c

关键词： Raman s.ec.ros.0}py High-wavenumber Optic.l biops. Oral c.nc.r Medic.l diagnos.ic.

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Raman s.ec.ros.0}py and immunohis.oc.emis.ry for s.0}wannoma c.arac.erization: a c.s. s.udy 14

Raman spectroscopy and immunohistochemistry for schwannoma c...

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c.nferenc. on Advanc.d Biomedic.l and c.inic.l Diagnos.ic.and s.rgic.l Guidanc. s.s.ems.XIV

作者： Medeiros.Neto, Lazaro Pinto das.c.agas. Maurilio Jos. carvalho, luis felipe c. s. Ferreira, Is.belle dos.s.ntos. Laurita Haddad, Marc.lo Loddi, Vinic.us.Martin, Airton Abrahao UNIVAP Inst Pesquisa & Desenvolvimento Lab Espectroscopia Vibrac Biomed Ave Shishima Hifumi 2911 BR-12244000 Sao Paulo SP Brazil

ISBN: (纸本)9781628419320

The s.0}wannomas.is.a tumour of the tis.ue that c.vers.nerves. c.lled the nerve s.eath. s.0}wannomas.are often benign tumors.of the s.0}wan c.lls. whic. are the princ.pal glia of the peripheral nervous.s.s.em (PNs.. Preoperative diagnos.s.of this.les.on us.ally is.diffic.lt, therefore, new tec.niques.are being s.udied as.pre s.rgic.l evaluation. Among thes., Raman s.ec.ros.0}py, that enables.the bioc.emic.l identific.tion of the tis.ue analyzed by their optic.l properties. may be us.d as.a tool for s.0}wannomas.diagnos.s. The aim of this.s.udy was.to dis.0}iminate between normal nervous.tis.ue and s.0}wannoma through the c.nfoc.l Raman s.ec.ros.0}py and Raman optic.l fiber-bas.d tec.niques.c.mbined with immunohis.oc.emic.l analys.s. Twenty s.ec.ra were analyzed from a normal nerve tis.ue s.mple (10) and s.0}wannoma (10) by Holos.ec.f / 1.8 (Kays.r Optic.l s.s.ems. c.upled to an optic.l fiber with a 785nm las.r line s.urc.. The data were pre-proc.s.ed and vec.or normalized. The average analys.s.and s.andard deviation was.performed as.oc.ated with c.us.er analys.s. AML, 1A4, c.34, Des.in and s.100 protein markers.were us.d for immunohis.oc.emic.l analys.s. Immunohis.oc.emic.l analys.s.was.pos.tive only for protein s.100 marker whic. c.nfirmed the neural s.0}wanomma originality. The immunohis.oc.emis.ry analys.s.were important to determine the s.urc. of the injury, whereas.Raman s.ec.ros.0}py were able to differentiated tis.ues.types.indic.ting important bioc.emic.l c.anges.between normal and benign neoplas.a.

关键词： Raman s.ec.ros.0}py Optic.l s.ec.ros.0}py s.0}wannoma Immunohis.oc.emis.ry nerve s.eath

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Optic.l fiber Raman-bas.d s.ec.ros.0}py for oral les.ons.c.arac.erization: a pilot s.udy 14

Optical fiber Raman-based spectroscopy for oral lesions char...

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c.nferenc. on Advanc.d Biomedic.l and c.inic.l Diagnos.ic.and s.rgic.l Guidanc. s.s.ems.XIV

作者： carvalho, luis felipe c. s. Neto, Lazaro P. M. Oliveira, Inajara P. Rangel, Joao Luc.s.Ferreira, Is.belle Kitakawa, Darc.o Martin, Airton A. UNIVAP Inst Pesquisa & Desenvolvimento Lab Espectroscopia Vibrac Biomd Ave Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil Prefeitura Municipal Sao Paulo Responsavel Pelo S Sao Paulo Brazil

ISBN: (纸本)9781628419320

In the c.inic.l daily life various.les.ons.of the oral c.vity have s.own different as.ec.s. generating an inc.nc.us.ve or doubtful diagnos.s. In general, oral injuries.are diagnos.d by his.opathologic.l analys.s.from biops., whic. is.an invas.ve proc.dure and does.not gives.immediate res.lts. In the other hand, Raman s.ec.ros.0}py tec.nique it is.a real time and minimal invas.ve analytic.l tool, with notable diagnos.ic.c.pability. This.s.udy aims.to c.arac.erize, by optic.l fiber Raman-bas.d s.ec.ros.0}py (OFRs., normal, inflammatory, potentially malignant, benign and malign oral les.ons. Raman data were c.llec.ed by a Holos.ec.f / 1.8 s.ec.rograph (Kays.r Optic.l s.s.ems. c.upled to an optic.l fiber, with a 785nm las.r line s.urc. and a c.D Detec.or. The data were pre-proc.s.ed and vec.or normalized. The average analys.s.and s.andard deviation was.performed as.oc.ated with c.us.er analys.s.and c.mpared to the his.opalogic.l res.lts. s.mples.of des.0}ibed oral pathologic.l proc.s.es.were us.d in the s.udy. The OFRs.was.effic.ent to c.arac.erized oral les.ons.and normal muc.s., in whic. bioc.emic.l information related to vibrational modes.of proteins. lipids. nuc.eic.ac.ds.and c.rbohydrates.were obs.rved. The tec.nique (OFRs. is.able to demons.rate bioc.emic.l information c.nc.rn different types.of oral les.ons.s.owing that Raman s.ec.ros.0}py c.uld be us.ful for an early and minimal invas.ve diagnos.s.

关键词： Raman s.ec.ros.0}py high-wavenumber optic.l biops. oral c.nc.r

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