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Searching for new ways of diagnosing and treating chronic fatigue syndrome in the context of intertwined immunopathology

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Asian Journal of Surgery 2025年

作者： chuwen Feng Tingting liu Shulin li Tiansong Yang Rehabilitation Department II The First Affiliated Hospital of Heilongjiang University of Chinese Medicine Harbin China Postdoctoral Research Station The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine Harbin China Heilongjiang Province Key Laboratory of Traditional Chinese Medicine Information Harbin China Graduate School of Heilongjiang University of Chinese Medicine Harbin China

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Identifying potential biomarkers in the hippocampus of chronic fatigue syndrome rats treated with moxibustion at Zusanli(ST36): a proteomics study

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Journal of Traditional Chinese Medicine 2025年第3期 571-585页

作者： FENG chuwen li Chaoran YANG Yan QU Yuanyuan SUN Zhongren SUN Weibo liU Tingting li Shulin YANG Tiansong Rehabilitation Department Ⅱ the First Affiliated Hospital of Heilongjiang University of Chinese Medicine Heilongjiang Province Key Laboratory of Traditional Chinese Medicine Information Department of Acupuncture Zhejiang Chinese Medical University College of Basic Medicine of Heilongjiang University of Chinese Medicine Graduate School of Harbin Medical University Graduate School of Heilongjiang University of Chinese Medicine

OBJECTIVE: To observe the effects of moxibustion at Zusanli(ST36) on rats with chronic fatigue syndrome(CFS) and to analyze the mechanisms of moxibustion through hippocampal Proteomics. METHODS: Male Sprague-Dawley(SD) rats were randomly divided into three groups: control group(CON), model group(MOD), and moxibustion group(MOX), with 12 rats in each group. The MOD and MOX groups underwent chronic multi-factor stress stimulation for 35 d to establish the CFS model. After modeling, the rats in the MOX group received mild moxibustion at Zusanli(ST36)(bilateral) for 10 minutes daily for 28 d. During the treatment period, rats in both the MOD and MOX groups continued modeling, while the CON group was kept under normal breeding conditions. The general condition of the rats was monitored, and behaviors were assessed using the Open Field Test(OFT), Exhaustion Treadmill Test, and Morris Water Maze(MWM). Hematoxylin and eosin(HE) staining and transmission electron microscopy(TEM) were employed to observe morphological changes in the hippocampus. Label-free Proteomics were utilized to identify differentially expressed proteins(DEPs) in the hippocampus, followed by bioinformatics analysis. The reliability of the Proteomics results was verified using Parallel Reaction Monitoring. RESULTS: A: Moxibustion at Zusanli(ST36) significantly reduced the general condition score of CFS rats, improved their behavioral performance in OFT, treadmill and MWM, and repaired the pathological and synaptic structural damage in the hippocampus.B: We identified DEPs by applying a fold change threshold of 1.2 and a significance level of P < 0.05. In the comparison between the CON and the MOD, we identified a total of 72 DEPs(31 up-regulated and 41 down-regulated) associated with the development of CFS. In the comparison between the MOX and the MOD group, we identified a total of 103 DEPs(40 up-regulated and 63 down-regulated) related to the therapeutic effects of moxibustion. Gene Ontology(GO) enrich

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Experimental investigation on rock breaking performance of cutter assisted with hydraulic fracturing

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ENGINEERING FRACTURE MECHANICS 2021年 248卷 107710-107710页

作者： li, Hongsheng liu, Songyong Zhu, Zhencai liu, Hao Zhang, Deyi Guo, chuwen China Univ Min & Technol Sch Mechatron Engn Xuzhou 221116 Jiangsu Peoples R China China Univ Min & Technol Jiangsu Collaborat Innovat Ctr Intelligent Min Eq Xuzhou 221008 Jiangsu Peoples R China China Univ Min & Technol Sch Elect Power Engn Xuzhou 221116 Jiangsu Peoples R China

To improve the efficiency of cutting hard rocks in roadway tunneling, a rock breaking method using a cutter assisted with hydraulic fracturing is proposed. In addition, the influence of the circumferential distribution of holes on the crack propagation was studied through a hydraulic fracturing experiment. On this basis, an experiment of rock breaking by a cutter assisted with hydraulic fracturing was conducted, and the effect of the hydraulic fracturing on the cutting performance was analyzed. The results show that the main cracks' initial direction and propagation path were influenced by the location and number of circumferentially distributed holes, around which the secondary cracks appeared. During the fracturing process, the main cracks expanded to the circumferentially distributed holes and developed into a crack network;the more empty holes there were, the denser the crack network was. Compared with the original rock samples, the cutting torque and propelling force of the non-directionally and directionally perforated fractured rock samples were significantly lower, and the cutting performance of the directionally perforated fractured rock samples was better. The research provides a possibility for the rapid excavation of extremely hard and abrasive rock.

关键词： Rock breaking Hydraulic fracturing Crack propagation Cutting performance

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CmNAC25 targets CmMYB6 to positively regulate anthocyanin biosynthesis during the post-flowering stage in chrysanthemum

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BMC BIOLOGY 2023年第1期21卷 211-211页

作者： Wang, Yuxi Wang, Yiguang Zhou, li-Jie Peng, Jialin Chen, chuwen liu, Shenhui Song, Aiping Jiang, Jiafu Chen, Sumei Chen, Fadi Nanjing Agr Univ State Key Lab Crop Genet & Germplasm Enhancement Key Lab Landscaping Coll HortMinist Agr & Rural AffairsKey Lab Biol Nanjing 210095 Peoples R China

Background Anthocyanin is a class of important secondary metabolites that determines colorful petals in chrysanthemum, a famous cut flower. 'Arctic Queen' is a white chrysanthemum cultivar that does not accumulate anthocyanin during the flowering stage. During the post-flowering stage, the petals of 'Arctic Queen' accumulate anthocyanin and turn red. However, the molecular mechanism underlying this flower color change remains unclear. Results In this study, by using transcriptome analysis, we identified CmNAC25 as a candidate gene promoting anthocyanin accumulation in the post-flowering stage of 'Arctic Queen'. CmNAC25 is directly bound to the promoter of CmMYB6, a core member of the MBW protein complex that promotes anthocyanin biosynthesis in chrysanthemum, to activate its expression. CmNAC25 also directly activates the promoter of CmDFR, which encodes the key enzyme in anthocyanin biosynthesis. CmNAC25 was highly expressed during the post-flowering stage, while the expression level of CmMYB#7, a known R3 MYB transcription factor interfering with the formation of the CmMYB6-CmbHLH2 complex, significantly decreased. Genetic transformation of both chrysanthemum and Nicotiana tabacum verified that CmNAC25 was a positive regulator of anthocyanin biosynthesis. Another two cultivars that turned red during the post-flowering stages also demonstrated a similar mechanism. Conclusions Altogether, our data revealed that CmNAC25 positively regulates anthocyanin biosynthesis in chrysanthemum petals during the post-flowering stages by directly activating CmMYB6 and CmDFR. Our results thus revealed a crucial role of CmNAC25 in regulating flower color change during petal senescence and provided a target gene for molecular design breeding of flower color in chrysanthemum.

关键词： Anthocyanin biosynthesis Flower color CmNAC25 MBW CmMYB6 Post-flowering stage Chrysanthemum

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藏鸡胚胎不同发育阶段腿肌组织中miRNA的分析及鉴定

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中国畜牧兽医 2025年第4期52卷 1681-1693页

作者：李志毅李洁陈楚雯农意王佳燕王孜吴锦波李志雄西南民族大学青藏高原动物遗传资源保护与利用教育部重点实验室成都610041 西南民族大学畜牧兽医学院成都610041 阿坝藏族羌族自治州畜牧科学技术研究所阿坝624402

【目的】探索藏鸡胚胎期与骨骼肌生长发育相关的微小RNA(miRNA),对其进行差异表达分析并构建miRNA-mRNA调控网络,为进一步了解miRNA在鸡肌肉生长发育过程中的调控机制提供理论基础。【方法】采集藏鸡胚胎期第10(E10)、14(E14)、18(E18)... 详细信息

【目的】探索藏鸡胚胎期与骨骼肌生长发育相关的微小RNA(miRNA),对其进行差异表达分析并构建miRNA-mRNA调控网络,为进一步了解miRNA在鸡肌肉生长发育过程中的调控机制提供理论基础。【方法】采集藏鸡胚胎期第10(E10)、14(E14)、18(E18)天腿肌组织进行转录组测序。通过生物信息学分析筛选出在3个日龄中均差异表达的miRNA,对差异表达的miRNA进行GO功能与KEGG通路富集分析,筛选与肌肉发育相关的mRNA和miRNA构建mRNA-miRNA调控网络,并随机选择6个差异表达的miRNAs进行实时荧光定量PCR验证。【结果】共有52个miRNAs在E10和E14中差异表达,137个miRNAs在E10和E18中差异表达,33个miRNAs在E14和E18中差异表达,有8个miRNAs在3个藏鸡不同时期胚胎腿肌中均差异表达。GO功能富集结果显示,与骨骼肌生长发育相关的条目有肌动蛋白结合、肌原纤维的组装、肌动球蛋白结构组织和基于肌动蛋白丝的过程、肌纤维膜等。KEGG通路富集结果发现,大量基因显著富集到与细胞的黏附、生存、迁移、增殖、代谢和分化、免疫调节等生物学功能有关的信号通路中。miRNA-mRNA调控网络结果显示,miRNA-210a-3p-MYO1D/PDliM3、miRNA-1662-DMD/SYNM、miRNA-184-3p-FHOD1等相互调控可能在鸡胚胎期肌肉生长发育过程中起重要作用。研究筛选出miRNA-133c-3p、miRNA-184-3p、miRNA-1662、miRNA-210a-3p、miRNA-1a-3p等miRNAs可能参与肌肉生长发育的过程。实时荧光定量PCR验证结果显示,6个miRNAs的表达趋势与转录组测序结果相一致。【结论】本研究共测得222个差异表达的miRNAs,其中有8个miRNAs在3个日龄中均差异表达,为后续miRNA在调控鸡胚胎时期肌肉生长发育的研究提供理论依据。

关键词：藏鸡胚胎期肌肉发育 miRNA 转录组测序

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InSb-Enhanced Thermally Tunable Terahertz Silicon Metasurfaces

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IEEE ACCESS 2019年 7卷 95087-95093页

作者： Yang, Daquan Zhang, Chao li, Xiaogang Lan, chuwen Beijing Univ Posts & Telecommun Sch Informat & Commun Engn State Key Lab Informat Photon & Opt Commun Beijing 100876 Peoples R China Southeast Univ State Key Lab Millimeter Waves Nanjing 210096 Jiangsu Peoples R China

Terahertz silicon-based all-dielectric metasurfaces have attracted enormous attention for their promising applications. In practice, however, their tuning ability has been limited by the stability of silicon. Herein, we propose a new way to realize thermally tunable silicon metasurfaces in the terahertz region based on InSb film. To verify the feasibility of this method, a tunable all-dielectric metasurface absorber based on hybrid dielectric waveguide resonance is designed and demonstrated. The absorber consists of sub-wavelength silicon cylinders on the polydimethylsiloxane (PDMS) substrate, and an ultra-thin InSb film is deposited on it to achieve tunability. Meanwhile, by employing the other free-standing grating structure, the universality of this method is demonstrated. Notably, when the temperature increases from 300 to 400 K, the resonance shift in the grating structure can reach 0.091 THz, and good amplitude stability in the transmission spectrum is achieved. With advantages like fine tunability and easy fabrication, these all-dielectric metasurfaces may have great potential in THz high efficiency devices.

关键词： Terahertz metamaterials optical devices semiconductor films temperature dependence thermal tunable circuits and devices

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Research progress on pathogenesis of chronic fatigue syndrome and treatment of traditional Chinese and Western medicine

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AUTONOMIC NEUROSCIENCE-BASIC & CliNICAL 2024年 255卷 103198-103198页

作者： liu, Tingting Sun, Weibo Guo, Shuhao Chen, Tao Zhu, Minghang Yuan, Zhiying li, Binbin Lu, Jing Shao, Yuying Qu, Yuanyuan Sun, Zhongren Feng, chuwen Yang, Tiansong Heilongjiang Univ Chinese Med Harbin Peoples R China Harbin Med Univ Harbin Peoples R China Heilongjiang Univ Chinese Med Rehabil Med Dept Affiliated Hosp 1 Harbin Peoples R China Key Lab Chinese Med Informot Heilongjiang Prov 24 Heping Rd Harbin Peoples R China

Chronic Fatigue Syndrome (CFS) is a complex and perplexing medical disorder primarily characterized by persistent and debilitating fatigue, often accompanied by a constellation of symptoms, including weakness, dyspnea, arthromyalgia, sore throat, and disrupted sleep patterns. CFS is defined by its persistent or recurrent manifestation for a minimum duration of six months, marked by an enduring and unrelenting fatigue that remains refractory to rest. In recent decades, this condition has garnered significant attention within the medical community. While the precise etiology of CFS remains elusive, it is postulated to be multifactorial. CFS is potentially associated with various contributory factors such as infections, chronic stress, genetic predisposition, immune dysregulation, and psychosocial influences. The pathophysiological underpinnings of CFS encompass viral infections, immune system dysregulation, neuroendocrine aberrations, heightened oxidative stress, and perturbations in gut microbiota. Presently, clinical management predominantly relies on pharmaceutical interventions or singular therapeutic modalities, offering alleviation of specific symptoms but exhibiting inherent limitations. Traditional Chinese Medicine (TCM) interventions have emerged as a promising paradigm, demonstrating notable efficacy through their multimodal, multi-target, multi-pathway approach, and holistic regulatory mechanisms. These interventions effectively address the lacunae in contemporary medical interventions. This comprehensive review synthesizes recent advancements in the understanding of the etiological factors, pathophysiological mechanisms, and interventional strategies for CFS, drawing from a corpus of domestic and international literature. Its aim is to furnish valuable insights for clinicians actively involved in diagnosing and treating CFS, as well as for pharmaceutical researchers delving into innovative drug development pathways. Moreover, it seeks to address the intric

关键词： Chronic fatigue syndrome Integrated traditional chinese and western medicine Review

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Deletion of the PtrDJ1C gene leads to increased branching in poplar

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Plant Physiology and Biochemistry 2025年 223卷 109789页

作者： Du, Jingxia Shao, Chunxue Wang, Dong Feng, Zizhuo Cui, chuwen li, Ruili Jewaria, Pawan Kumar Wang, Xuanyi Xiao, Jianwei Wang, Xinwei State Key Laboratory of Efficient Production of Forest Resources Beijing Forestry University Beijing 100083 China College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 China College of Agriculture and Forestry Hebei North University Zhangjiakou 075000 China National Institute of Plant Genome Research Aruna Asaf Ali Marg 10531 India

The PtrDJ1C gene is essential for poplar growth and early chloroplast development. Disruption of PtrDJ1C expression results in an albino leaf phenotype and increased branching. However, the underlying mechanism for the increased branching remains unknown. In this study, we employed integrated approaches to investigate the function of PtrDJ1C in the branch-increasing phenotype. Our results revealed that levels of indole-3-acetic acid (IAA), gibberellin (GA), and abscisic acid (ABA) were significantly reduced in ptrdj1c mutants, while cytokinin (CK) levels were slightly increased. Transcriptomic and proteomic analyses identified several key genes and proteins involved in hormone regulation and branching development that were differentially expressed. Specifically, the expression levels of TAA, ZEP, and GA20ox—genes involved in IAA, GA, and ABA biosynthesis—were significantly reduced in ptrdj1c, while IPT and LOG, which regulate CK synthesis, were upregulated. Moreover, immunoblot analysis further validated reduced levels of key biosynthetic enzymes for IAA, GA, and ABA, alongside increased levels of IPT and LOG enzymes. Interestingly, our findings suggest that hormone signaling pathways act in concert with the transcription factor WUSCHEL (WUS) to synergistically promote branching development. These results provide novel insight into the regulatory role of PtrDJ1C in hormone balance and its downstream effects on poplar branching. © 2025 Elsevier Masson SAS

关键词： Chloroplast Hormone Plant branching Poplar PtrDJ1C

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Deep sea sediments associated with cold seeps are a subsurface reservoir of viral diversity

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ISME JOURNAL 2021年第8期15卷 2366-2378页

作者： li, Zexin Pan, Donald Wei, Guangshan Pi, Weiling Zhang, chuwen Wang, Jiang-Hai Peng, Yongyi Zhang, Lu Wang, Yong Hubert, Casey R. J. Dong, Xiyang Sun Yat Sen Univ Sch Marine Sci Zhuhai Peoples R China Florida Gulf Coast Univ Water Sch Dept Ecol & Environm Studies Ft Myers FL USA Third Inst Oceanog Key Lab Marine Genet Resources Minist Nat Resources Xiamen Peoples R China Westlake Univ Sch Engn Key Lab Coastal Environm & Resources Zhejiang Pro Hangzhou Peoples R China Westlake Inst Adv Study Inst Adv Technol Hangzhou Peoples R China Chinese Acad Sci Inst Deep Sea Sci & Engn Dept Life Sci Sanya Peoples R China Univ Calgary Dept Biol Sci Calgary AB Canada

In marine ecosystems, viruses exert control on the composition and metabolism of microbial communities, influencing overall biogeochemical cycling. Deep sea sediments associated with cold seeps are known to host taxonomically diverse microbial communities, but little is known about viruses infecting these microorganisms. Here, we probed metagenomes from seven geographically diverse cold seeps across global oceans to assess viral diversity, virus-host interaction, and virus-encoded auxiliary metabolic genes (AMGs). Gene-sharing network comparisons with viruses inhabiting other ecosystems reveal that cold seep sediments harbour considerable unexplored viral diversity. Most cold seep viruses display high degrees of endemism with seep fluid flux being one of the main drivers of viral community composition. In silico predictions linked 14.2% of the viruses to microbial host populations with many belonging to poorly understood candidate bacterial and archaeal phyla. Lysis was predicted to be a predominant viral lifestyle based on lineage-specific virus/host abundance ratios. Metabolic predictions of prokaryotic host genomes and viral AMGs suggest that viruses influence microbial hydrocarbon biodegradation at cold seeps, as well as other carbon, sulfur and nitrogen cycling via virus-induced mortality and/or metabolic augmentation. Overall, these findings reveal the global diversity and biogeography of cold seep viruses and indicate how viruses may manipulate seep microbial ecology and biogeochemistry.

关键词： Environmental microbiology Microbial ecology

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Resveratrol improves left ventricular remodeling in chronic kidney disease via Sirt1-mediated regulation of FoxO1 activity and MnSOD expression

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BIOFACTORS 2020年第1期46卷 168-179页

作者： li, Peipei Song, Xiaoli Zhang, Dingwu Guo, Naifeng Wu, chuwen Chen, Kairen liu, Yue Yuan, li Chen, Xiaolan Huang, Xinzhong Nantong Univ Affiliated Hosp Dept Nephrol 20 Xisi Rd Nantong 226001 Jiangsu Peoples R China Tradit Chinese Med Hosp Tongzhou Dist Dept Nephrol Nantong Jiangsu Peoples R China

Left ventricular remodeling commonly complicates end-stage renal disease following chronic kidney disease (CKD). This study investigated the therapeutic efficacy of resveratrol (RSV), a polyphenolic compound, on left ventricular remodeling in subtotal nephrectomy rats and sought to uncover the underlying molecular mechanisms. Subtotal nephrectomy caused renal dysfunction, such as gradual increases in serum creatinine and blood urea nitrogen, glomerular sclerosis, and tubulointerstitial fibrosis. In addition, subtotal nephrectomy also resulted in significant increases in myocyte cross-sectional area, interstitial and perivascular fibrosis, and left ventricular dilatation. All these detrimental effects were alleviated in the presence of RSV. Mechanistically, RSV treatment led to the upregulation of manganese-containing superoxide dismutase (MnSOD) in the heart. Coimmunoprecipitation studies showed that silent information regulator 1 (Sirt1) bound forkhead box protein O1 (FoxO1) and thus reduced acetylated FoxO1. RSV strengthened this interaction between Sirt1 and FoxO1. Loss of one allele of Sirt1 aggravated renal damage, myocyte hypertrophy, and interstitial fibrosis in nephrectomized mice. Taken together, our data show that Sirt1 is an important mediator for the protective roles of RSV on renal and heart damage in CKD rodent model, and FoxO1 and MnSOD are likely downstream targets of Sirt1. Therefore, Sirt1 might be a potential therapeutic target for the treatment of left ventricular remodeling caused by CKD.

关键词： chronic kidney disease FoxO1 left ventricular remodeling MnSOD resveratrol Sirt1

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