检索结果-内蒙古大学图书馆

Design, development, and validation of a strand-specific RT-qPCR assay to differentiate replicating versus nonreplicating Rabies virus

引用

JOURNAL OF VIROLOGICAL METHODS 2025年 332卷 115054页

作者： Xiang, Hong Hong, Chao Tian, Yuan Gao, Yu cun, yina Zhao, Hongling Li, Guilan Chen, Yu Zhou, Jian Chinese Acad Med Sci & Peking Union Med Coll Inst Med Biol Kunming Peoples R China

The Rabies virus, a single-strand RNA virus with a negative-sense polarity, is responsible for causing encephalitis and is a zoonotic disease. If not promptly treated after infection, it has a close to 100 % fatality rate. Similar to other negative-sense polarity single-strand RNA viruses, the Rabies virus requires the creation of a positive-strand RNA intermediate for replication. One approach to identify this replication activity is to detect the complementary strand of the viral RNA genome in suspected infected cells or tissues. The reported Rabies virus RT-qPCR detection methods are designed to detect total viral load in samples without distinguishing between positive- and negative-strand for RNA viruses. As such, in this study, a sensitive Taqman-based strand-specific RT-qPCR assay has been developed to quantitatively detect both the positive- and negative-strand of the Rabies virus. This method demonstrates good reproducibility across a wide dynamic range and exhibits linearity of 8 logs with a lower limit of detection of 103 copies/mu L for the positive-strand and 9 logs with a lower limit of detection of 102 copies/mu L for the negative-strand. Notably, it can accurately detect a specific viral RNA strand even in the presence of high levels of the opposite strand, confirming the method's specificity. In summary, a reliable strandspecific RT-qPCR assay has been developed and validated to differentiate replicating from non-replicating Rabies virus.

关键词： Rabies virus RT-qPCR Strand-specific RT-qPCR Single-strand RNA virus Virus detection

来源：评论

学校读者我要写书评

暂无评论

Association of Renin Binding Protein(RnBP) Gene Polymorphisms with Essential Hypertension in the Hani Minority of Southwestern China

引用

Journal of Genetics and Genomics 2013年第8期40卷 433-436页

作者： Yilong Dong Yun Ding yina cun Chunjie Xiao Laboratory for Conservation and Utilization of Bio-resources and Human Genetics Center School of MedicineYunnan University

Essential hypertension is one of the most common multi- factorial diseases, affecting 20%--30% of the human popula- tion （Ibrahim and Damasceno, 2012）. Based on the results of twin studies, adoption studies and statistical analyses of blood pressure （BP） across various pedigrees, it has been estimated that 30%--50% of the variability in blood pressure among the general population is genetically determined （Garcia et al., 2003）. Although the genetic mechanisms of essential hyper- tension have not been studied well, investigations for the genes that constitute the renin-angiotensin system （RAS） appear to be particularly promising, since this system plays a central role in the regulation of blood pressure （Ferrario, 2010）.

关键词： Association of Renin Binding Protein RnBP Gene Polymorphisms with Essential Hypertension in the Hani Minority of Southwestern China gene SNPs

来源：评论

学校读者我要写书评

暂无评论

Construction High-Yield Candidate Influenza Vaccine Viruses in Vero Cells by Reassortment

引用

JOURNAL OF MEDICAL VIROLOGY 2016年第11期88卷 1914-1921页

作者： Yu, Wei Yang, Fan Yang, Jinghui Ma, Lei cun, yina Song, Shaohui Liao, Guoyang Chinese Acad Med Sci Inst Med Biol Dept Biol Prod 5 935th Jiaoling Rd Kunming 650118 Yunnan Province Peoples R China Peking Union Med Coll 935th Jiaoling Rd Kunming 650118 Yunnan Province Peoples R China Kunming Gen Hosp Chengdu Mil Reg Dept Pharmaceut Kunming Yunnan Peoples R China

Usage of influenza vaccine is the best choice measure for preventing and conclusion of influenza virus infection. Although it has been used of chicken embryo to produce influenza vaccine, following with WHO recommended vaccine strain, there were uncontrollable factors and its deficiencies, specially, during an influenza pandemic in the world. The Vero cells are used for vaccine production of a few strains including influenza virus, because of its homology with human, recommended by WHO. However, as known most of the influenza viruses strains could not culture by Vero cells. It was used two high-yield influenza viruses adapted in Vero cells as donor viruses, such as A/Yunnan/1/2005Va (H3N2) and B/Yunnan/2/2005Va (B), to construct high-yield wild influenza virus in Vero cells under antibody selection pressure. After reassortment and passages, it obtained the new Vaccine strains with A/Tianjin/15/2009Va (H1N1), A/Fujian/196/2009Va (H3N2) and B/Chongqing/1384/2010Va (B), which was not only completely keeping their original antigenic (HA and NA), but also grown well in Vero cells with highyield. All results of gene analysis and HA, HI shown that this reassortment method could be used to find new direction to product the influenza vaccine. (C) 2016 Wiley Periodicals, Inc.

关键词： influenza vaccine Vero cells adaption reassortment

来源：评论

学校读者我要写书评

暂无评论

Reassortment of high-yield influenza viruses in vero cells and safety assessment as candidate vaccine strains

引用

HUMAN VACCINES & IMMUNOTHERAPEUTICS 2017年第1期13卷 111-116页

作者： Zhou, Jian Yang, Fan Yang, Jinghui Ma, Lei cun, yina Song, Shaohui Liao, Guoyang Chinese Acad Med Sci Inst Med Biol Dept Biol Prod 5 935th Jiaoling Rd Kunming 650118 Yunnan Province Peoples R China Peking Union Med Coll 935th Jiaoling Rd Kunming 650118 Yunnan Province Peoples R China First Hosp Yunnan Prov Kunming Yunnan Peoples R China

Vaccination is the practiced and accessible measure for preventing influenza infection. Because chicken embryos used for vaccine production have various insufficiencies, more efficient methods are needed. African green monkey kidney (Vero) cells are recommended by the World Health Organization (WHO) as a safe substitute for influenza vaccine production for humans. However, the influenza virus usually had low-yield in Vero cells, which limits the usage of Vero cellular vaccines. This study used 2 high-yield influenza viruses in Vero cells: A/Yunnan/1/2005Va (H3N2) and B/Yunnan/2/2005Va (B) as donor viruses. It used 3 wild strain viruses to reassort new adaptation viruses, including: A/Tianjin/15/2009(H1N1), A/Fujian/196/2009(H3N2), and B/Chongqing/1384/2010(B). These three new viruses could maintain the characteristic of high-yield in Vero cells. Furthermore, they could keep the immunogenic characteristics of the original wild influenza viruses. Importantly, these viruses were shown as safe in chicken embryo and guinea pigs assessment systems. These results provide an alternative method to produce influenza vaccine based on Vero cells.

关键词： influenza virus reassortant safety assessment Vero cells

来源：评论

学校读者我要写书评

暂无评论

COVID-19 coronavirus vaccine T cell epitope prediction analysis based on distributions of HLA class I loci (HLA-A, -B, -C) across global populations

引用

HUMAN VACCINES & IMMUNOTHERAPEUTICS 2021年第4期17卷 1097-1108页

作者： cun, yina Li, Chuanyin Shi, Lei Sun, Ming Dai, Shuying Sun, Le Shi, Li Yao, Yufeng Chinese Acad Med Sci & Peking Union Med Coll Yunnan Key Lab Vaccine Res & Dev Severe Infect Di Yunnan Engn Res Ctr Vaccine Res & Dev Severe Infe Inst Med Biol Kunming Yunnan Peoples R China Kunming Med Univ Sch Basic Med Sci Kunming Yunnan Peoples R China

T cell immunity, such as CD4 and/or CD8 T cell responses, plays a vital role in controlling the virus infection and pathological damage. Several studies have reported SARS-CoV-2 proteins could serve as ideal vaccine candidates against SARS-CoV-2 infection by activating the T cell responses. In the current study, based on the SARS-CoV-2 sequence and distribution of host human leukocyte antigen (HLA), we predicted the possible epitopes for the vaccine against SARS-CoV-2 infections. Firstly, the current study retrieved the SARS-CoV-2 S and N protein sequences from the NCBI Database. Then, using the Immune Epitope Database Analysis Resource, we predicted the CTL epitopes of the SARS-CoV-2 S and N proteins according to worldwide frequency distributions of HLA-A, -B, and -C alleles (>1%). Our results predicted 90 and 106 epitopes of N and S proteins, respectively. Epitope cluster analysis showed 16 and 34 respective clusters of SARS-CoV-2 N and S proteins, which covered 95.91% and 96.14% of the global population, respectively. After epitope conservancy analysis, 8 N protein epitopes and 6 S protein epitopes showed conservancy within two SARS-CoV-2 types. Of these 14 epitopes, 13 could cover SARS coronavirus and Bat SARS-like coronavirus. The remaining epitope (KWPWYIWLGF(1211-1220)) could cover MERS coronavirus. Finally, the 14-epitope combination could vaccinate 89.60% of all individuals worldwide. Our results propose single or combined CTL epitopes predicted in the current study as candidates for vaccines to effectively control SARS-CoV-2 infection and development.

关键词： SARS-CoV-2 Human Leukocyte Antigen (HLA) T cell epitopes population coverage vaccine

来源：评论

学校读者我要写书评

暂无评论

Origination and evolution of a human-specific transmembrane protein gene, c1orf37-dup

引用

HUMAN MOLECULAR GENETICS 2006年第11期15卷 1870-1875页

作者： Yu, Haijing Jiang, Huifeng Zhou, Qi Yang, Jufen cun, yina Su, Bing Xiao, Chunjie Wang, Wen Chinese Acad Sci Key Lab Cellular & Mol Evolut Kunming Inst Zool Kunming 650223 Yunnan Peoples R China Chinese Acad Sci Kunming Primate Res Ctr Kunming 650223 Yunnan Peoples R China Yunnan Univ Key Lab Bioresources Conservat & Utilizat Kunming 650091 Peoples R China Yunnan Univ Ctr Human Genet Kunming 650091 Peoples R China Chinese Acad Sci Max Planck Jr Res Grp Kunming 650223 Peoples R China Chinese Acad Sci Grad Sch Beijing 100039 Peoples R China

A transmembrane protein gene, c1orf37-dup, was identified as a young gene specific to humans. It was derived from the conserved c1orf37 gene through retroposition after the divergence of human and chimpanzee. This gene has evolved rapidly driven by positive Darwinian selection as evident from a significantly high ratio of non-synonymous substitution rate to synonymous substitution rate (K-a/K-s=2.08) between the new c1orf37-dup and the parental c1orf37 genes. Population genetics analysis disclosed a very low level of polymorphism in the c1orf37-dup gene and its neighboring regions, thus providing support for the occurrence of a recent selective sweep. The GFP experiments revealed that it encodes a transmembrane protein associated with cell membranes. Non-random distribution of amino acid changes indicates the C1ORF37-DUP protein may have evolved diverged functions in the presumably functionally important N-terminal region in the cytoplasm and the extracellular loop. These lines of evidence support that the functional adaptation of c1orf37-dup has occurred in humans. Unlike its ubiquitously expressed parental gene, c1orf37-dup expresses selectively in several human tissues including brain. It is suggested that c1orf37-dup encodes a novel transmembrane protein in humans which potentially endows new properties to cell surface interactions.

关键词： Genes Proteins Human

来源：评论

学校读者我要写书评

暂无评论

Analyzed immunogenicity of fractional doses of Sabin-inactivated poliovirus vaccine (sIPV) with intradermal delivery in rats

引用

HUMAN VACCINES & IMMUNOTHERAPEUTICS 2016年第12期12卷 3125-3131页

作者： Ma, Lei Cai, Wei Sun, Mingbo cun, yina Zhou, Jian Liu, Jing Hu, Wenzhu Zhang, Xinwen Song, Shaohui Jiang, Shude Liao, Guoyang Chinese Acad Med Sci Peking Union Med Coll Inst Med Biol Dept Biol Prod 5 935th Jiaoling Rd Kunming 650118 Yunnan Province Peoples R China

Objective: The live-attenuated oral polio vaccine (OPV) will be no longer used when wild poliovirus (WPV) eliminating in worldwide, according to GPEI (the Global Polio Eradication Initiative) Reports. It is planning to replace OPV by Sabin-based inactivated poliovirus vaccine (sIPV) in developing countries, with purpose of reducing of the economic burden and maintaining of the appropriate antibody levels in population. It studied serial fractional doses immunized by intradermal injection (ID) in rats, to reduce consume of antigen and financial burden, maintaining sufficient immunogenicity;Methods: Study groups were divided in 4 groups of dose gradient, which were one-tenth (1/10), one-fifth (1/5), one-third (1/3) and one full dose (1/1), according to the volume of distribution taken from the same batch of vaccine (sIPV). Wistar rats were injected intradermally, with the needle and syringe sing the mantoux technique taken once month for 3 times. It was used as positive control that intramuscular inoculation (IM) was injected with one-full dose (1/1) with same batch of sIPV. PBS was used as negative control. Blood samples were collected via tail vein. After 30 d with 3 round of immunization, it analyzed the changes of neutralization antibody titers in the each group by each immunization program end;Results: The results of seroconversion had positive correlation with different doses in ID groups. The higher concentration of D-antigen (D-Ag) could conduct higher seroconversion. Furthermore, different types of viruses had different seroconversion trend. It showed that the geometric mean titers (GMTs) of each fractional-dose ID groups increased by higher concentration of D-Ag, and it got significant lower than the full-dose IM group. At 90th days of immunization, the GMTs for each poliovirus subtypes of fractional doses were almost higher than 1:8, implied that it could be meaning positive seroprotection titer for polio vaccine types, according to WHO suggestion;Conclusio

关键词： fractional dose intradermal delivery Sabin IPV

来源：评论

学校读者我要写书评

暂无评论

人乳头瘤病毒治疗性疫苗临床试验研究进展

引用

中华预防医学杂志 2023年第10期57卷 1647-1654页

作者：王泯驿寸怡娜中国医学科学院北京协和医学院医学生物学研究所昆明650118

子宫颈癌已经成为严重威胁女性健康的公共卫生问题,人乳头瘤病毒(HPV)感染是子宫颈癌最主要致病因素。为了预防HPV感染,目前使用的预防性疫苗主要通过诱导宿主产生体液免疫反应,从而生成中和抗体。相较之下,HPV治疗性疫苗的设计目标是... 详细信息

子宫颈癌已经成为严重威胁女性健康的公共卫生问题,人乳头瘤病毒(HPV)感染是子宫颈癌最主要致病因素。为了预防HPV感染,目前使用的预防性疫苗主要通过诱导宿主产生体液免疫反应,从而生成中和抗体。相较之下,HPV治疗性疫苗的设计目标是诱导宿主产生以Th1为主的细胞免疫应答,并清除已有的病毒感染并延缓或抑制肿瘤的发展。目前,已有多种基于不同机制和技术的HPV治疗性疫苗进入临床试验阶段。本文对上述疫苗的临床试验进展进行了梳理和总结,以期为我国HPV治疗性疫苗的研发工作提供参考。

关键词：人乳头瘤病毒宫颈肿瘤治疗性疫苗临床试验

来源：评论

学校读者我要写书评

暂无评论

Are the beta-adducin C1797T polymorphism and gamma-adducin A386G polymorphism associated with essential hypertension in Yi and Hani populations?

引用

CLINICA CHIMICA ACTA 2006年第1-2期374卷 153-154页

作者： Zhou, Xuhong Tang, Wenru Wu, Hngyan cun, yina Yang, Yang Zhang, Jiali Yu, Haijing Li, Kaiyuan He, Li Xiao, Chunjie Yunnan Univ Ctr Human Genet Kunming 650091 Yunnan Peoples R China Yunnan Univ Lab Conservat & Utilizat Bioresources Kunming 650091 Yunnan Peoples R China

来源：评论

学校读者我要写书评

暂无评论

Association of WNK1 exon 1 polymorphisms with essential hypertension in Hani and Yi minorities of China

引用

Journal of Genetics and Genomics 2011年第4期38卷 165-171页

作者： yina cun Jin Li Wenru Tang Xiaozhi Sheng Haijing Yu Bingrong Zheng Chunjie Xiao Laboratory for Conservation and Utilization of Bio-resources and Human Genetics Center of Yunnan UniversityKunming 650091China

The association of polymorphisms in exon 1 of the WNK1 gene with essential hypertension in the minority groups of Hani and Yi of China was investigated in the case-control *** sequence of 1257 bp containing the WNK1 gene exon 1 was determined in 1307 individuals（649 essential hypertension subjects and 658 controls）to identify SNPs in Hani and Yi minority *** of eleven previously known SNPs （rs3168640,rs11885,rs11554421 and rs34880640）were *** SNP analysis indicated that SNPs rs11885 and rs11554421 were significantly associated with hypertension in both Hani and Yi populations,and rs34880640 was significantly associated with hypertension in Hani but not in Yi population,adjusted for *** analysis indicated that the haplotype H1 significantly decreased the risk of hypertension in both *** results suggested that WNK1 polymorphisms were involved in the predisposition of essential hypertension in Hani and Yi populations and its effects showed a clear population *** finding supported the importance of population specificity in determining the genetic factors associated with diseases and thus disease treatment.

关键词： WNK1 SNPs Haplotype Essential hypertension Hani population Yi population

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：