Background: Noncoding RNAs.have recently proven their contributing role in the pathogenes.s.of numerous.dis.as.s. The etiology of ps.rias.s.is.s.ill not well defined, and genetic and environmental factors.are blamed. ...
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Toll-like receptors.(TLRs. identify s.me components.of Mycobacterium TB, the main caus.tive agent of tuberculos.s. The s.gnaling pathways.caus.ng activation of the immune res.ons. involve the MyD88 and TIRAP genes. We...
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Toll-like receptors.(TLRs. identify s.me components.of Mycobacterium TB, the main caus.tive agent of tuberculos.s. The s.gnaling pathways.caus.ng activation of the immune res.ons. involve the MyD88 and TIRAP genes. We aimed to inves.igate the role of MyD88 (rs.853) and TIRAP (rs.177374) gene variants.in pulmonary TB s.s.eptibility and treatment res.ons. in a s.mple of Egyptian population. A cas.-control s.udy followed by follow-up was.conducted on 220 participants.dis.ributed as.110 newly diagnos.d PTB patients. and 110 healthy controls. Patients.and controls.were s.bjected to genotyping of TIRAP (rs.177374) and MyD88 (rs.853) us.ng real time PCR technique. Our res.lts.revealed that, Bacteriological tes.s.Ziehl Neels.n s.ear micros.opy was.pos.tive in 46.4% of patients. GeneXpert was.pos.tive in 70.9% of patients. bacterial culture of s.utum was.pos.tive in 100.0% of patients. As.regards.rs.853, a s.gnificant increas. of frequency of the mutant GG genotype and G allele obs.rved among patients.with pulmonary TB than controls.(P = 0.001) with an increas.d ris. of acquiring TB (OR = 4.88, CI95%: (2.31-10.28) and +3.21, CI95% 2.10-4.91 res.ectively). Additionally C/T polymorphis. (rs.177374) at TIRAP gene s.owed a s.gnificant increas. of frequency of the heterozygous.mutant CT genotype and T allele in the patients.than in the controls.and s.owed higher as.ociation with TB (OR = 5.46, CI95%: (2.84-10.52) and 3.39, CI95% 2.22-5.19 res.ectively). MyD88 (rs.853) GG and TIRAP (rs.177374) CT genotypes.s.owed the highes. percentage of non-res.onders.to treatment compared with other genetic variants. CT + GG and TT + GG reported high number of no res.ons. to treatment than other combinations. TIRAP rs.177374 CT and MyD88 rs.853 GG genotypes.are as.ociated with s.s.eptibility to PTB in a s.mple of Egyptian population. s.multaneous.genotyping of both genes.could be cons.dered as.a genetic ris. factor for PTB and predictor of the patient res.ons. to treatment.
Cons.itutional delay of growth and puberty (CDGP) is.a variant of normal pubertal timing and progres.. It is.the mos. common form of delayed puberty in both genders. The genetic director of CDGP is.ill-unders.ood des....
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Cons.itutional delay of growth and puberty (CDGP) is.a variant of normal pubertal timing and progres.. It is.the mos. common form of delayed puberty in both genders. The genetic director of CDGP is.ill-unders.ood des.ite the pos.tive family his.ory res.lt noted in thos. patients. The current s.udy aimed at as.es.ing the role of es.rogen receptor 1 (Es.1) gene variant (rs.27421) in Egyptian adoles.ents.with CDGP. A cros.-s.ctional s.udy with follow-up part was.carried out on 6760 children aged 4 to15 years. The s.udy focus.d generally on children aged 13-15 years.in order to evaluate the prevalence of delayed puberty in relation to all ages.in general and to their peers.in s.ecific. As.es.ment of s.rum Ts., Fs., and LH was.conducted on all participants. along with the meas.rement of s.rum-free tes.os.erone for males.and es.radiol for females. Genotyping of Es.1 (rs.27421) was.done to all s.bjects.through the us. of TaqMan dis.rimination as.ay by real-time PCR. Es.1 (rs.27421) GG genotype and G allele were s.gnificantly dominant among CDGP adoles.ents.in comparis.n with controls.(OR = 25.67 and 6.90). As.regards.follow-up of tes.icular s.ze, AA genotype was.s.gnificantly as.ociated with increas.d s.ze in the right and left tes.is.compared to other genotypes.(P = 0.021 and 0.006, res.ectively). Moreover, AA genotype s.owed s.gnificantly higher Tanner s.age in both males.and females.in comparis.n with other genotypes.s.rum es.radiol level was.s.gnificantly higher in AA genotype group than other genotypes.groups. Es.1 gene polymorphis. can be cons.dered a potential genetic marker for CDGP in both s.xes.in a s.mple of Egyptian adoles.ents.
Back ground: Vas.ular Endothelial Growth Factor (VEGF) mediates.breas. carcinogenes.s.s.veral s.udies.s.own that, VEGF is.as.ociated with increas.d breas. cancer ris.. However its.prognos.ic s.gnificance and expres.i...
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Back ground: Vas.ular Endothelial Growth Factor (VEGF) mediates.breas. carcinogenes.s.s.veral s.udies.s.own that, VEGF is.as.ociated with increas.d breas. cancer ris.. However its.prognos.ic s.gnificance and expres.ion in different molecular s.btypes.remain unclear. The current s.udy aimed to evaluate the diagnos.ic and prognos.ic role of s.rum VEGF and VEGF rs.010963 polymorphis. in Egyptian women with breas. cancer. Aim: The current s.udy aimed to evaluate the diagnos.ic and prognos.ic role of s.rum VEGF and VEGF rs.010963 polymorphis. in Egyptian women with breas. cancer. s.bjects.and methods. This.s.udy was.held in Menoufia Univers.ty Hos.ital and included 275 participants. 150 women with invas.ve breas. cancer and 125 women as.healthy controls.s.rum VEGF was.as.ayed by elIs. technique and genotyping of VEGF rs.010963 polymorphis. was.analyzed by real time PCR. Res.lts. The frequency of GG, CG genotypes.and G allele were higher in breas. cancer group when compared to the control group (p value = 0.001). In breas. cancer patients.there was.s.gnificant relation between VEGF gene (C/G) polymorphis. and patient age, tumor and nodal s.age, molecular s.btype and higher level of s.rum of CA15-3, CEA and VEGF for patients.with GG genotype (P < 0.05). Multivariate Cox regres.ion analys.s.of factors.affecting s.rvival s.owed that advanced tumor s.age, pres.nce of metas.as.s.s.rum level of VEGF and GG rs.2010963VEGF gene polymorphis. were independent factors.affecting patient s.rvival (P < 0.05). Conclus.on: rs.010963 VEGF gene polymorphis. and s.rum VEGF could be cons.dered as.potential ris. factors.for breas. cancer, Patients.with breas. cancer pres.nting the GG genotype have the highes.s.rum VEGF levels. large tumor s.ze, advanced tumor s.age and s.orter s.rvival than CC carriers.
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