ISL1 plays a key role in several cancers, including pheochromocytoma, gastrointestinal, pancreatic, and lung tumors and bile duct carcinoma. In order to elucidate the role of ISL1 in breast cancer, we performed quanti...
详细信息
ISL1 plays a key role in several cancers, including pheochromocytoma, gastrointestinal, pancreatic, and lung tumors and bile duct carcinoma. In order to elucidate the role of ISL1 in breast cancer, we performed quantitative real-time polymerase chain reaction and Western blotting analysis, and we found that ISL1 was upregulated in breast cancer cells and tissues. Moreover, high expression of ISL1 was correlated with tumor size, metastasis, and poor prognosis. Colony formation analysis and CCK-8 analysis revealed that ISL1 facilitated breast cancer cell proliferation. In addition, wound healing analysis and transwell invasion analysis demonstrated that ISL1 played a role in cell migration and invasion. Interestingly, the expression of ISL1 was also associated with the expression of vascular endothelial growth factor (VEGF) in breast cancer, and ISL1 promoted angiogenesis in breast cancer. In conclusion, reducing the expression of ISL1 suppresses proliferation, migration, invasion, and angiogenesis in breast cancer, suggesting that ISL1 might serve as a novel molecular therapy target in breast cancer.
Molecular engineering of covalent organic frameworks (COFs) offers an alternative approach to conventional anthraquinone oxidation via photo-induced H 2 O 2 production from O 2 reduction. Despite their potential, repo...
详细信息
Molecular engineering of covalent organic frameworks (COFs) offers an alternative approach to conventional anthraquinone oxidation via photo-induced H 2 O 2 production from O 2 reduction. Despite their potential, reported photocatalysts suffer limited proton mobility, low selectivity, and insufficient charge separation and utilization. Herein, we report a nitroxyl radical (TEMPO) decorated two-dimensional (2D) donor-acceptor (D-A)-COF photocatalyst via a one-pot strategy. Under visible light irradiation, highly crystalline TAPP-TPDA-TEMPO-COF ( TT-T-COF ) exhibits a remarkable photocatalytic H 2 O 2 yield of 10066 μmol g −1 h −1 in two-phase water-benzyl alcohol (10 % BA) system through direct two-electron (2 e − ) pathway. The mechanistic study by DFT calculations and in situ DRIFT spectra suggests Yeager-type adsorption of *O 2 ⋅ − intermediate on the nitroxyl radical site (N−O⋅). The efficient photocatalytic performance and stability of TT-T-COF are attributed to the involvement of the nitroxyl radical, which enhances selective O 2 adsorption, establishes a distinct electron density distribution, and facilitates photogenerated charge separation compared to TT-HT-COF and TT-COF counterparts. This study uncovers a new perspective for constructing metal-free, redox-mediated radical-based COFs for sustainable energy conversion, storage, and biomedical applications.
This paper considers the problem of distributed H-infinity filtering for a class of switched repeated scalar nonlinear systems with randomly occurred sensor nonlinearities and asynchronous switching due to practical r...
详细信息
This paper considers the problem of distributed H-infinity filtering for a class of switched repeated scalar nonlinear systems with randomly occurred sensor nonlinearities and asynchronous switching due to practical reasons. The possibility of randomly occurred sensor nonlinearities is described by a Bernoulli stochastic variable, and the asynchronous switching filtering means that the mode of the plant is different from the mode of the designed filter possibly. A distributed filtering network is used to estimate the system state instead of a filter to improve reliability in case of faults of the filter. A distributed mode-dependent filter is designed by constructing a unified mode-dependent Lyapunov function and solving a set of linear matrix inequalities. Some novel sufficient conditions are obtained by using the average dwell time switching mechanism such that the augmented filtering error system is stochastically exponentially stable and achieves a prescribed H-infinity disturbance attention index. Finally, a numerical example is provided to demonstrate the effectiveness of the proposed designed method.
Enzalutamide, a second-generation androgen receptor (AR) antagonist, has demonstrated clinical benefit in men with prostate cancer. However, it only provides a temporary response and modest increase in survival, indic...
Enzalutamide, a second-generation androgen receptor (AR) antagonist, has demonstrated clinical benefit in men with prostate cancer. However, it only provides a temporary response and modest increase in survival, indicating a rapid evolution of resistance. Previous studies suggest that enzalutamide may function as a partial transcriptional agonist, but the underlying mechanisms for enzalutamide-induced transcription remain poorly understood. Here, we show that enzalutamide stimulates expression of a novel subset of genes distinct from androgen-responsive genes. Treatment of prostate cancer cells with enzalutamide enhances recruitment of pioneer factor GATA2, AR, Mediator subunits MED1 and MED14, and RNA Pol II to regulatory elements of enzalutamide-responsive genes. Mechanistically, GATA2 globally directs enzalutamide-induced transcription by facilitating AR, Mediator and Pol II loading to enzalutamide-responsive gene loci. Importantly, the GATA2 inhibitor K7174 inhibits enzalutamide-induced transcription by decreasing binding of the GATA2/AR/Mediator/Pol II transcriptional complex, contributing to sensitization of prostate cancer cells to enzalutamide treatment. Our findings provide mechanistic insight into the future combination of GATA2 inhibitors and enzalutamide for improved AR-targeted therapy.
暂无评论