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Reliable breast cancer miRNAs detection with enhanced silicon nanowire biosensor by PNA probe and optical calibration

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SENSORS AND ACTUATORS B-CHEMICAL 2024年 401卷

作者： Gao, Anran wang, fuwen Fan, Zishuo Chen, Shixing Wu, Kejin East China Normal Univ Dept Elect Key Lab Polar Mat & Devices MOE Shanghai 200241 Peoples R China Fudan Univ Shanghai Inst Infect Dis & Biosecur Shanghai 200433 Peoples R China Fudan Univ Obstet & Gynecol Hosp Dept Breast Surg Shanghai 200011 Peoples R China Geno Biotech Co Ltd Shanghai 200300 Peoples R China

MicroRNAs (miRNAs) have attracted significant attention in the field of cancer research as a promising class of biomarkers. However, precise, sensitive, and specific detection of miRNAs still confronts challenges due to their dynamic expression, low abundance, and high sequence similarity among families. The highly sensitive silicon nanowires (SiNWs) biosensors are limited by little interface discrepancy within the SiNWs, which has the potential to affect the final output results. In this study, the calibration of the SiNWs biosensor was initially suggested to be conducted through photo response. This approach successfully mitigated the impact of preparation and modification procedures, resulting in an enhanced correlation between the biosensor and q-PCR for the identification of breast cancer miRNA. Specifically, the correlation coefficient was raised from below 0.5 to above 0.8. Furthermore, the uncharged peptide nucleic acid (PNA) was used for device modification in order to address the issue of detecting miRNAs with a total length in below 0.01xPBS buffer. It was shown that the PNA probe exhibited greater sensitivity compared to the DNA probe in 0.001xPBS buffer. Significantly, the improved biosensor exhibited favorable selectivity and was capable of identifying single base mismatch. The sensor exhibited a high level of sensitivity in detecting miRNA within a concentration range of 1 fM to 10 pM when applied to actual human blood samples. The biosensor exhibited an excellent level of reproducibility in the analysis of spiked samples, achieving a recovery rate of 91 %, without requiring RNA extraction or amplification procedures. The biosensor has the capability to directly detect miRNA in authentic clinical samples, hence demonstrating significant promise for the timely diagnosis of cancer via the use of miRNA as biomarkers.

关键词： SiNWs Breast cancer Photo response MiRNAs Sensitivity

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Crystal Face-Dependent Behavior of Single-Atom Pt: Construct of SA-FLP Dual Active Sites for Efficient NO2 Detection

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ADVANCED SCIENCE 2024年第29期11卷 e2402038页

作者： Ou, Yucheng wang, Bing Xu, Nana Song, Quzhi Liu, Tao Xu, Hui wang, fuwen wang, Yingde Natl Univ Def Technol Coll Aerosp Sci & Engn Sci & Technol Adv Ceram Fiber & Composites Lab Changsha 410073 Peoples R China

The strong potential of platinum single atom (Pt-SA) in gas sensor technology is primarily attributed to its high atomic economy. Nevertheless, it is imperative to conduct further exploration to understand the impact of Pt-SA on the active sites. In this study, the evolution of Pt-SA on (100)CeO2 and (111)CeO2 is examined, revealing notable disparities in the position and activity of surface Pt-SA on different crystal planes. The Pt-SA in (100)CeO2 surface can enhance the stability of Ce3+ and construct a frustrated Lewis pair (FLP) to form a double active site by combining the steric hindrance effect of oxygen vacancies, which increases the response value from 1.8 to 27 and reduce the response-recovery time from 140-192 s to 25-26 s toward five ppm NO2 at room temperature. Conversely, Pt-SA tends to bind to terminal oxygen on the surface of (111)CeO2 and become an independent reaction site. The response value of Pt-SA-(111)CeO2 surface only increased from 1.6 to 3.8. This research underscores the correlation between single atoms and crystal plane effects, laying the groundwork for designing and synthesizing ultra-stable and efficient gas sensors.

关键词： CeO2 gas sensor FLP NO2 detection Pt single atom

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Metabolomic profiling of Dezhou donkey seminal plasma related to freezability

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REPRODUCTION IN DOMESTIC ANIMALS 2022年第10期57卷 1165-1175页

作者： wang, Zhaofei Yu, Jie Zhong, Shuai Fan, Zhaobin wang, fuwen Ji, Chuanliang wang, Yantao Lei, Chuzhao Dang, Ruihua Zhao, Fuwei Northwest A&F Univ Coll Anim Sci & Technol Key Lab Anim Genet Breeding & Reprod Shaanxi Prov Xianyang 712100 Shaanxi Peoples R China Dong EE Jiao Co Ltd Natl Engn Res Ctr Gelatin Based Tradit Chinese Me Done E Country Peoples R China Heze Univ Coll Pharm Heze Peoples R China

Donkeys are indispensable livestock in China because they have transport function and medicinal value. With the popularization of artificial insemination on donkeys, semen cryopreservation technology has gradually become a research hotspot. Seminal plasma is a necessary medium for transporting sperm and provides energy and nutrition for sperm. Seminal plasma metabolites play an important role in the process of sperm freezing, and also have an important impact on sperm motility and fertilization rate after freezing and thawing. In this study, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was used to compare the metabolic characteristics of seminal plasma of high freezability (HF) and low freezability (LF) male donkeys. We identified 672 metabolites from donkey seminal plasma, of which 33 metabolites were significantly different between the two groups. Metabolites were identified and categorized according to their major chemical classes, including homogeneous non-metal compounds, nucleosides, nucleotides, and analogues, organosulphur compounds, phenylpropanoids and polyketide, organoheterocyclic compounds, organic oxygen compounds, benzenoids, organic acids and derivatives, lipids and lipid-like molecules, organooxygen compounds, alkaloids and derivatives, organic nitrogen compounds. The results showed that the contents of phosphatidylcholine, piceatannol and enkephalin in donkey semen of HF group were significantly higher than those of LF group (p < .05), while the contents of taurocholic and lysophosphatidic acid were significantly lower than those of LF group (p < .05). The different metabolites were mainly related to sperm biological pathway response and oxidative stress. These metabolites may be considered as candidate biomarkers for different fertility in jacks.

关键词： cryopreservation donkey freezability LC-MS MS metabolites seminal plasma

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A Forecasting Method of Photovoltaic Power Generation Based on NeuralProphet and BiLSTM 10

A Forecasting Method of Photovoltaic Power Generation Based ...

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IEEE 10th International Conference on Information, Communication and Networks (ICICN)

作者： Xiao, JianJun Li, Feng wang, fuwen Liu, Gaohe wang, Xin Liu, Qiong wang, Liang Fu, Qi State Grid Gansu Elect Power Corp Dingxi Power Supply Co Dingxi Peoples R China Lanzhou Univ Technol Sch Comp & Commun Lanzhou Peoples R China

ISBN: (纸本)9781665490825

Aiming at the problem that the traditional photovoltaic power generation prediction model cannot fully mine the effective information contained in photovoltaic data, which leads to the low accuracy of photovoltaic power generation prediction, an short-term forecasting method based on NeuralProphet (NP) and bidirectional long short-term memory network (BiLSTM) is proposed. The NP is used to mine the time series characteristics of photovoltaic data and acquire the potential trend part and seasonal part hidden in the data. Then the results are merged with the original data and the three-dimensional data is constructed in a sliding window manner as the input of the BiLSTM model. Finally, the BiLSTM is used to realize the short-term prediction of photovoltaic power generation in the next six hours. The experimental results show that the proposed prediction model can effectively improve the prediction accuracy of short-term photovoltaic power generation compared with traditional machine learning methods.

关键词： photovoltaic power generation forecasting short-term bidirectional long short-term memory network NeuralProphet

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Metabolomic profiling of Dezhou donkey sperm associated with freezability

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THERIOGENOLOGY 2022年 181卷 131-139页

作者： Yu, Jie wang, Zhaofei An, Xiaoya Liu, Haibing wang, fuwen Ji, Chuanliang Liu, Guiqin wang, Yantao He, Fei Dang, Ruihua Zhao, Fuwei Northwest A&F Univ Coll Anim Sci & Technol Key Lab Anim Genet Breeding & Reprod Shaanxi Prov Xianyang 712100 Shaanxi Peoples R China Heze Univ Coll Pharm 2269 Daxue Rd Heze 274015 Peoples R China Dong EE Jiao Co Ltd Natl Engn Res Ctr Gelatin Based Tradit Chinese Me 78 E Jiao St Liaocheng 252201 Shandong Peoples R China Liaocheng Univ Technol Collaborat Innovat Ctr Shandong Donkey Ind Liaocheng 252201 Shandong Peoples R China Shangdong Agr Univ Coll Anim Sci & Technol Tai An 271018 Shandong Peoples R China Liaocheng Anim Husb & Vet Tech Serv Ctr Liaocheng Shandong Peoples R China

The cryopreservation technology of sperm has promoted the popularization of artificial insemination in the reproductive process of donkeys to a certain extent, but the freezing-thawing process would bring damage to the sperm, and the vitality of the sperm would be greatly reduced after freezing. Sperm metabolites play an important role in the process of sperm freezing, and also have an important impact on the vitality and fertilization rate of sperm after freezing-thawing. In this study, the LC-MS/MS analysis method was used to compare the metabolic profiles of high freezability (HF) and low freezability (LF) male donkey sperm after freezing-thawing. We identified 1323 metabolites in total, of which 17 metabolites are significantly different between the two groups. Most of these metabolites belong to fatty acids and phospholipids, including phosphatidylcholine, stearic acid and so on. These different metabolites are mostly related to the plasma membrane fatty acids of sperm and oxidative stress. Our results illustrate several metabolites related to sperm freezability and provide corresponding biomarkers. (c) 2022 Elsevier Inc. All rights reserved.

关键词： Freezability Male donkey LC-MS MS Cryopreservation Metabolites Sperm

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2022年我国城市轨道交通数据统计与发展分析

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隧道建设（中英文） 2023年第3期43卷 521-528,I0076-I0083页

作者：王福文冯爱军北京城建设计发展集团股份有限公司北京100037

首先,对我国城市轨道交通运营线路及里程进行统计,结果显示:截至2022年12月31日,全国拥有轨道交通的城市有55个,运营总里程约10078 km(若含“智轨”系统,则有58个城市,运营总里程10176 km)。然后,对我国城市轨道交通新增建设规划批复情... 详细信息

首先,对我国城市轨道交通运营线路及里程进行统计,结果显示:截至2022年12月31日,全国拥有轨道交通的城市有55个,运营总里程约10078 km(若含“智轨”系统,则有58个城市,运营总里程10176 km)。然后,对我国城市轨道交通新增建设规划批复情况进行统计,结果显示:2022年,国家发改委批复2座城市的新一轮建设规划,共计12条线路,211.8 km,投资约1817.3亿元;批复3座城市的建设规划调整方案,涉及7条线路,99.2 km,投资增加约526.8亿元,新增及调整项目所涉及的线路制式均为地铁制式。最后,分析我国城市轨道交通行业技术发展特点,认为:1)国家陆续出台的相关政策凸显对城市轨道交通发展的重视;2)规划发展突出与城市发展及多层次交通系统的融合协同;3)中国土木工程学会轨道交通分会评选出城市轨道交通创新推广技术,这些技术代表了近年来准确的技术发展方向,包括土建技术侧重解决复杂特殊地质条件下的施工工艺工法、智慧建造技术贯穿于城市轨道交通对的全生命周期、更加重视运营改造及低碳环保技术的研发及应用。

关键词：城市轨道交通运营线路交通系统国家发改委特殊地质条件数据统计技术发展方向土建技术

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Changes in sperm metabolites of Dezhou donkey after cryopreservation

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REPRODUCTION IN DOMESTIC ANIMALS 2022年第12期57卷 1593-1601页

作者： Yu, Jie wang, Zhaofei wang, fuwen wang, Wenhao Ge, Shihao Fan, Zhaobin Liu, Bing Li, Min Dong, Boying Zhao, Fuwei Dang, Ruihua Northwest A&F Univ Coll Anim Sci & Technol Key Lab Anim Genet Breeding & Reprod Shaanxi Prov Xianyang Shaanxi Peoples R China Heze Univ Coll Pharm Heze Peoples R China Dong E E Jiao Co Ltd Natl Engn Res Ctr Gelatin based Tradit Chinese Me Xinxiang Henan Peoples R China

Sperm cryopreservation technology has laid the foundation for promoting the popularity of artificial insemination in donkey reproduction, but the freeze-thaw process can cause sperm damage, and the viability of frozen sperm is greatly reduced, resulting in low insemination ability. Sperm metabolites play an important role in the freezing process of spermatozoa and have a major influence on the freezability of spermatozoa. The aim of this study was to explore the differential metabolites in donkey spermatozoa before and after cryopreservation by liquid chromatography-tandem mass spectrometry (LC-MS/MS). We analysed ejaculate samples from male donkeys obtained before and after freezing and identified 1323 metabolites. Compared with fresh sperm (F), the metabolites of cryopreserved sperm (CRY) were significantly changed, and 570 metabolites were significantly different between the two groups (p < .05). Among them, 277 metabolites were higher in frozen sperm, while the opposite was true for 293 metabolites. These metabolites mainly include phospholipids, lysophospholipids and amino acids., most of which are associated with oxidative stress and sperm capacitation. We describe significantly different metabolites before and after freezing that are significantly associated with decreased sperm motility post-freezing and can be used as biomarkers of decreased sperm motility post-freezing.

关键词： cryopreservation liquid chromatography-tandem mass spectrometry male donkey metabolites sperm

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电网故障下永磁直驱风电机组并网电流的自抗扰控制

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上海交通大学学报 2024年第12期58卷 1968-1976页

作者：王晗王富文周党生施刚张建文蔡旭上海交通大学电力传输与功率变换控制教育部重点实验室上海200240 鲁能新能源(集团)有限公司北京100020 深圳市禾望电气股份有限公司广东深圳518055

电网并网导则要求风电机组在电网故障时具备快速无功支撑能力,采用传统比例积分控制器控制的直驱风力机在应对电网阻抗变化和模型严重扰动时存在暂态响应速度慢、适应性差的问题.针对该问题,提出一种改进的线性自抗扰控制器与最小方差... 详细信息

电网并网导则要求风电机组在电网故障时具备快速无功支撑能力,采用传统比例积分控制器控制的直驱风力机在应对电网阻抗变化和模型严重扰动时存在暂态响应速度慢、适应性差的问题.针对该问题,提出一种改进的线性自抗扰控制器与最小方差滤波器相结合的无功支撑控制策略.采用最小方差滤波器快速精准检测含背景谐波的故障电网电压幅值,可有效减小故障检测延时;利用线性自抗扰控制器有效提升机组暂态无功响应速度及其在电网阻抗扰动下的控制适应性和鲁棒性.搭建基于PSCAD/EMTDC软件的直驱风力机仿真模型,考虑弱电网和电网含背景谐波两种场景,对提出控制策略的暂态支撑有效性进行了仿真验证.

关键词：直驱风电机组电网故障自抗扰控制最小方差滤波器

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2023年我国城市轨道交通数据统计与发展分析

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隧道建设（中英文） 2024年第2期44卷 393-400,I0073-I0080页

作者：王福文梁帅文冯爱军中国土木工程学会轨道交通分会北京100037 北京城建设计发展集团股份有限公司北京100037

首先,对我国城市轨道交通运营线路及里程进行统计,结果显示:截至2023年12月31日,全国拥有轨道交通的城市有61个,运营总里程约11 034 km(若不含“智轨”系统,则有54个城市,运营总里程10 866 km)。然后,对我国城市轨道交通新增建设规划批... 详细信息

首先,对我国城市轨道交通运营线路及里程进行统计,结果显示:截至2023年12月31日,全国拥有轨道交通的城市有61个,运营总里程约11 034 km(若不含“智轨”系统,则有54个城市,运营总里程10 866 km)。然后,对我国城市轨道交通新增建设规划批复情况进行统计,结果显示:2023年,国家发展和改革委批复5个城市的轨道交通建设规划或建设规划调整方案,新增运营线路长度最多的仍为地铁制式。最后,分析我国城市轨道交通行业技术发展特点,认为:1)高质量发展是城市轨道交通管理的主基调;2)优质工程及技术创新比往年有所增加;3)城市轨道交通仍保持较快速度发展,行业更关注已开通线路的运营和维保。可以预期,绿色低碳建造技术、智慧高效管控技术将是今后行业发展的重点方向。

关键词：轨道交通运营线路数据统计发展分析

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A novel 31bp deletion within the CDKL5 gene is significantly associated with growth traits in Dezhou donkey

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ANIMAL BIOTECHNOLOGY 2021年第3期34卷 503-507页

作者： wang, fuwen wang, Gang Dalielihan, Baligen wang, Zhaofei Chang, Tingjin Yang, Ge Lei, Chuzhao Dang, Ruihua Northwest A&F Univ Coll Anim Sci & Technol Key Lab Anim Genet Breeding & Reprod Shaanxi Prov Xianyang Shaanxi Peoples R China

The discovery of molecular markers which associate with livestock economic traits is of great significance for livestock breeding. Selective analysis has found a potential correlation between CDKL5 and growth traits, but there is still a lack of experimental proof. In this study, a 31-bp deletion (g.176595_176626delATGTCACATGTGGTACTGCCATGTGGAATTT) of CDKL5 gene was found by sequencing. The 31-bp indel was then genotyped in 380 individuals of Dezhou donkeys by polyacrylamide gel electrophoresis and there were three genotypes in this population. After the association analysis between growth traits and genotypes, it was found that this 31-bp indel polymorphism was significantly associated with the chest circumference of Dezhou donkeys (p < 0.05), and body length, chest depth and rump width (p < 0.01). In addition, all individuals with DD genotype were better than those with other genotypes in growth traits. This study revealed that a newly identified polymorphic locus in the CDKL5 gene is related to growth traits, which provides a molecular marker for genetic improvement of Dezhou donkey and may lay a solid foundation for the breeding of Dezhou donkey.

关键词： CDKL5 Dezhou donkey deletion growth traits

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