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A Rise in ROS and EPS Production: New Insights into the Trichodesmium erythraeum Response to Ocean Acidification

JOURNAL OF PHYCOLOGY 2021年第1期57卷 172-182页

作者： Wu, Shijie Mi, Tiezhu Zhen, Yu Yu, Kaiqiang wang, fuwen Yu, Zhigang Ocean Univ China Minist Educ Key Lab Marine Environm & Ecol Qingdao 266100 Peoples R China Pilot Natl Lab Marine Sci & Technol Qingdao Lab Marine Ecol & Environm Sci Qingdao 266237 Peoples R China Ocean Univ China Coll Environm Sci & Engn Qingdao 266100 Peoples R China Ocean Univ China Inst Adv Ocean Study Minist Educ Key Lab Marine Chem Theory & Technol Qingdao 266100 Peoples R China

The diazotrophic cyanobacterium Trichodesmium is thought to be a major contributor to the new N in parts of the oligotrophic, subtropical, and tropical oceans. In this study, physiological and biochemical methods and transcriptome sequencing were used to investigate the influences of ocean acidification (OA) on Trichodesmium erythraeum (T. erythraeum). We presented evidence that OA caused by CO2 slowed the growth rate and physiological activity of T. erythraeum. OA led to reduced development of proportion of the vegetative cells into diazocytes which included up-regulated genes of nitrogen fixation. Reactive oxygen species (ROS) accumulation was increased due to the disruption of photosynthetic electron transport and decrease in antioxidant enzyme activities under acidified conditions. This study showed that OA increased the amounts of (exopolysaccharides) EPS in T. erythraeum, and the key genes of ribose-5-phosphate (R5P) and glycosyltransferases (Tery_3818) were up-regulated. These results provide new insight into how ROS and EPS of T. erythraeum increase in an acidified future ocean to cope with OA-imposed stress.

关键词： EPS ocean acidification ROS Trichodesmium erythraeum

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浅埋暗挖交叉相贯隧道施工力学行为试验研究

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建筑科学与工程学报 2024年第2期41卷 115-123页

作者：崔凌岳王福文孙铁成中铁十八局集团第一工程有限公司河北涿州072750 北京城建设计发展集团股份有限公司北京100037 石家庄铁道大学省部共建交通工程结构力学行为与系统安全国家重点实验室河北石家庄050043 石家庄铁道大学道路与铁道工程安全保障省部共建教育部重点实验室河北石家庄050043

为完善浅埋暗挖交叉相贯隧道在施工过程中的受力形式及变形特征,依托青岛某地铁工程,根据所选地铁车站的地质条件及衬砌结构特点浇筑衬砌模型并配制围岩相似材料,利用模型试验对采用全断面法在Ⅴ级围岩环境下进行的风道和车站主体开挖... 详细信息

为完善浅埋暗挖交叉相贯隧道在施工过程中的受力形式及变形特征,依托青岛某地铁工程,根据所选地铁车站的地质条件及衬砌结构特点浇筑衬砌模型并配制围岩相似材料,利用模型试验对采用全断面法在Ⅴ级围岩环境下进行的风道和车站主体开挖过程的力学行为展开研究,并从隧道衬砌结构变形及地层内部位移变化规律两方面分析了浅埋暗挖交叉相贯隧道全断面开挖施工的力学行为。结果表明:对于交叉相贯位置处的隧道结构,已有隧道结构将改变后续施工隧道衬砌结构应变最大值及其出现位置;位于交叉相贯隧道结构附近不同象限内的围岩分别处于水平变形累加区和往复区;地表竖向变形最大点位于后续施工的车站上方且与交叉相贯位置存在一定距离,而在交叉相贯位置处的地表竖向变形值稍小;模型试验明确了交叉相贯隧道在施工过程中围岩及衬砌结构的变形特征,给出了隧道结构地表最大变形与施工步序间的时空关系;研究结果可为相关交叉相贯隧道结构的施工及设计提供依据。

关键词：地下结构交叉相贯隧道模型试验浅埋暗挖地表沉降

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Global dispersal and adaptive evolution of domestic cattle:a genomic perspective

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Stress Biology 2023年第1期3卷 98-110页

作者： Xiaoting Xia Kaixing Qu Yan wang Mikkel-Holger SSinding fuwen wang Quratulain Hanif Zulfiqar Ahmed Johannes A.Lenstra Jianlin Han Chuzhao Lei Ningbo Chen Key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China Academy of Science and Technology Chuxiong Normal UniversityChuxiong 675000China Qingdao Municipal Bureau of Agriculture and Rural Affairs Qingdao 266000China Section for Computational and RNA Biology Department of BiologyUniversity of CopenhagenCopenhagen 1350Denmark National Institute for Biotechnology and Genetic Engineering FaisalabadPakistan Faculty of Veterinary and Animal Sciences University of Poonch RawalakotAzad Jammu and Kashmir 12350Pakistan Faculty of Veterinary Medicine Utrecht UniversityUtrechtThe Netherlands Livestock Genetic Program International Livestock Research Institute(ILRI)Nairobi 00100Kenya CAAS-ILRI Joint Laboratory On Livestock and Forage Genetic Resources Institute of Animal ScienceChinese Academy of Agricultural Sciences(CAAS)Beijing 100193China

Domestic cattle have spread across the globe and inhabit variable and unpredictable *** have been exposed to a plethora of selective pressures and have adapted to a variety of local ecological and management conditions,including UV exposure,diseases,and stall-feeding *** selective pressures have resulted in unique and important phenotypic and genetic differences among modern cattle breeds/*** efforts to sequence the genomes of local and commercial cattle breeds/populations,along with the growing availability of ancient bovid DNA data,have significantly advanced our understanding of the genomic architecture,recent evolution of complex traits,common diseases,and local adaptation in ***,we review the origin and spread of domestic cattle and illustrate the environmental adaptations of local cattle breeds/populations.

关键词： Cattle Origin Domestication Migration route Environmental adaptation Selective pressure

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Overall fabrication of uniform BN interphase on 2.5D-SiC fabric via precursor-derived methods

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VACUUM 2024年 230卷

作者： wang, fuwen Zhang, Xiaoshan Du, Yiang Ou, Yucheng Song, Quzhi Liu, Tao Wu, Xiaohan Tan, Shixiang wang, Bing Natl Univ Def Technol Coll Aerosp Sci & Engn Sci & Technol Adv Ceram Fiber & Composites Lab Changsha 410073 Peoples R China

Boron nitride (BN) interphase plays a crucial role in silicon carbide fiber-reinforced silicon carbide (SiCf/SiC) composites, because it directly influences the mechanical properties and high-temperature resistance of SiCf/SiC composites. However, fabricating a high-quality BN interphase on SiC fabrics with low cost and high efficiency remains a significant challenge. In this study, a uniform and dense BN interphase was overally prepared on 2.5DSiC fabric by precursor-derived method. Whether it is the surface fiber or the inner fiber of the 2.5D-SiC fabric, the interphase thickness remains consistent at 550 +/- 30 nm. Moreover, the obtained BN exhibits a lower crystallization temperature (approximately 1200 degrees C). Additionally, it maintains a strong bond with the fabric after high-temperature treatment at 1600 degrees C in an argon atmosphere and also retains robust adhesion after hightemperature vacuum treatment at 1600 degrees C. This study not only presents the overall fabrication of a uniform BN interphase on SiC fabric but also provides a novel strategy for preparing interphases on various types of fabrics.

关键词： BN interphase Precursor-derived methods SiC fabrics

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Damping Control for Drivetrain Torsional Vibration Attenuation of Grid Forming Wind Turbines with DFIG

Damping Control for Drivetrain Torsional Vibration Attenuati...

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Power System and Green Energy Conference (PSGEC)

作者： Ziteng Guo Yu Zhang Chen Zhang Haoxiang Zong fuwen wang Xu Cai Key Laboratory of Control of Power Transmission and Conversion of Ministry of Education Shanghai Jiao Tong University Shanghai China Shanghai Zhonglv New Energy Technology Co. Ltd Shanghai China

ISBN: (数字)9798350365573

ISBN: (纸本)9798350365580

Research on grid-forming (GFM) control strategies for wind turbines (WTs) has primarily focused on the electromagnetic characteristics of the WTs, overlooking the impact on the drivetrain. This limitation has hindered the application of GFM control strategies in engineering. To fill this gap, a two-mass drivetrain model is built to assess the influence of the bandwidth of the virtual synchronous generator (VSG) control strategy on drivetrain torsional vibration characteristics. A comparison of torsional vibration characteristics between the GFM-WT and traditional grid-following WT (GFL-WT) highlights the need for increased attention to drivetrain torsional vibration in GFM-WT. Then, the inadequacy of the traditional drivetrain torsional vibration suppression strategy (T-DTVSS) when applied in VSG control is examined. Finally, a novel drivetrain torsional vibration suppression strategy named virtual synchronous phase angle compensation drivetrain torsional vibration suppression strategy (VSPAC-DTVSS) is proposed, and the efficacy of this strategy is validated through simulation using PSCAD.

关键词： Vibrations Green energy Grid following Bandwidth Attenuation Synchronous generators Wind turbines PSCAD Frequency control Grid forming

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先驱体转化法制备氮化硼陶瓷纤维研究进展

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硅酸盐学报 2024年第9期52卷 2875-2888页

作者：杜贻昂张韵铂宋曲之王福稳谭士湘王兵王应德国防科技大学空天科学学院新型陶瓷纤维及其复合材料重点实验室长沙410073

氮化硼纤维具有低密度、高强度、耐高温、强绝缘和高透波等特性,是空天飞行器所需高性能透波复合材料的增强纤维。先驱体转化法是氮化硼纤维最具潜力的制备方法之一。目前,高性能氮化硼纤维的制备是该领域的主要挑战,其组成结构调控与... 详细信息

氮化硼纤维具有低密度、高强度、耐高温、强绝缘和高透波等特性,是空天飞行器所需高性能透波复合材料的增强纤维。先驱体转化法是氮化硼纤维最具潜力的制备方法之一。目前,高性能氮化硼纤维的制备是该领域的主要挑战,其组成结构调控与构效关系引起了广泛关注。当前研究的重点集中在氮化硼纤维工艺优化上,包括先驱体分子组成结构优化、热处理工艺和纤维微结构调控。本文结合国内外研究现状,在介绍先驱体转化法的基础上,从分子结构及其构效关系的角度切入,分别从氮化硼先驱体的合成和调控方法以及纤维热处理与纤维微结构调控等方面综述了最新的研究进展,并展望了氮化硼纤维的未来发展方向。

关键词：氮化硼陶瓷纤维先驱体转化法分子结构调控热处理

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mRNA sequencing provides new insights into the pathogenesis of Hirschsprung's disease in mice

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PEDIATRIC SURGERY INTERNATIONAL 2023年第1期39卷 268-268页

作者： Yang, Qiwen wang, fuwen wang, Zhaofei Guo, Jiajun Chang, Tingjin Dalielihan, Baligen Yang, Ge Lei, Chuzhao Dang, Ruihua Northwest A&F Univ Coll Anim Sci & Technol Key Lab Anim Genet Breeding & Reprod Shaanxi Prov Xianyang 712100 Shaanxi Peoples R China

Purpose The aim of this study is to use RNA sequencing and RT-qPCR to identify the main susceptibility genes linked to the occurrence and development of Hirschsprung disease in the colonic tissues of EDNRBm1yzcm and wild *** RNA was extracted from colon tissues of 3 mutant homozygous mice and 3 wild mice. RNA degradation, contamination concentration, and integrity were then measured. The extracted RNA was then sequenced using the Illumina platform. The obtained sequence data are filtered to ensure data quality and compared to the reference genome for further analysis. DESeq2 was used for gene expression analysis of the raw data. In addition, graphene oxide enrichment analysis and RT-qPCR validation were also *** This study identified 8354 differentially expressed genes in EDNRBm1yzcm and wild mouse colon tissues by RNA sequencing, including 4346 upregulated genes and 4005 downregulated genes. Correspondingly, the results of RT-qPCR analysis showed good correlation with the transcriptome data. In addition, GO and KEGG enrichment results suggested that there were 8103 terms and 320 pathways in all DEGs. When P < 0.05, 1081 GO terms and 320 KEGG pathways reached a significant level. Finally, through the existing studies and the enrichment results of differentially expressed genes, it was determined that axon guidance and the focal adhesion pathway may be closely related to the occurrence of *** This study analyzed and identified the differential genes in colonic tissues between EDNRBm1yzcm mice and wild mice, which provided new insight for further mining the potential pathogenic genes of Hirschsprung's disease.

关键词： Hirschsprung's disease RNA-seq EDNRB Colon

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Responses of triacylglycerol synthesis inSkeletonema marinoito nitrogen and phosphate starvations¹

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JOURNAL OF PHYCOLOGY 2020年第6期56卷 1505-1520页

作者： Zhang, Mei Xing, Yongze wang, fuwen Mi, Tiezhu Zhen, Yu Ocean Univ China Coll Environm Sci & Engn Qingdao 266100 Peoples R China Pilot Natl Lab Marine Sci & Technol Qingdao Lab Marine Ecol & Environm Sci Qingdao 266237 Peoples R China Minist Educ Key Lab Marine Environm & Ecol Qingdao 266100 Peoples R China Minist Nat Resources Inst Oceanog 4 Beihai 536002 Peoples R China

Skeletonema marinoiis one of the most widespread marine planktonic diatoms in temperate coastal regions and sometimes can form massive blooms. Yet, the molecular mechanisms of triacylglycerol (TAG) synthesis in nutrient-deficient conditions for this species are still unknown. This study aimed to investigate how the TAG biosynthetic pathway ofS. marinoireacts to the culture age and nitrogen (N) or phosphorus (P) deficiency at molecular levels. Meanwhile, we also described the physiological and biochemical changes ofS. marinoiin response to N or P starvation over time. To obtain reliable qRT-PCR data, six putative reference genes were identified for assessing expression stability using geNorm and BestKeeper software, andActinexhibited the most stable expression across 45 ***. We found that the expression of TAG biosynthesis-related genes andACCaseenzyme activity varied in response to the different nutrient conditions and culture age. Taken together, we speculated that the capacity of TAG biosynthesis inS. marinoiis induced by N or P stress, and increases with culture age. Furthermore, TAG biosynthesis appears to respond more strongly to P deficiency than to N deficiency. Our study provides important insights into how diatoms regulate the TAG biosynthetic pathway when stressed by nutrient limitation. Besides, the data obtained from this study also provide useful clues for further exploring genes that can be used for metabolic engineering to enhance lipid production.

关键词： Skeletonema marinoi triacylglycerol synthesis nitrogen phosphorus

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富镍镨掺杂镍钛形状记忆合金的微结构、相变特性与硬度

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稀有金属材料与工程 2021年第7期50卷 2352-2357页

作者：赵石磊赵昆王富文包头医学院内蒙古包头014040

采用真空熔炼法向NiTi二元合金中掺杂Pr稀土元素,制备了多组分原子分数的Ni_(50)Ti_(50)-xPrx(x=0,0.1,0.3,_(0.5),0.7,0.9)合金。研究了Pr元素的添加对NiTi合金金相组织、相变温度和硬度的影响。结果表明,Ni_(50)Ti_(50)-xPrx合金由NiTi基体与NiPr夹杂相组成,其中Ni_(50)Ti_(49.5)Pr_(0.5)合金的马氏体相变温度达73℃,合金的热滞窄至37℃,维氏硬度约为28_(50)MPa。Pr元素的添加显著降低了NiTi合金的马氏体相变温度,同时,与其他NiTi基合金相比,NiTiPr合金保持了较窄的热滞和较高的硬度。

关键词：镍钛镨微观结构相变硬度

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Microstructure and phase transition characteristics of NiTi shape memory alloy

Microstructure and phase transition characteristics of NiTi ...

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2020 International Conference on Advanced Materials and Intelligent Manufacturing and Advanced Steel for Automotive Seminar, ICAMIM 2020

作者： Shilei, Zhao Kun, Zhao fuwen, wang Baotou Medical College Baotou Innr Mongolia China

Shape memory alloy (SMA) with shape memory effect and superelasticity has had an increasing interest for researchers of mechanics of materials in recent decades. With the deepening of theoretical research, the applications of SMA have also made considerable progress. SMA has a unique solid phase transformation characteristic that is thermoelastic martensitic transformation. The high temperature phase of SMA is austenite, while the low temperature phase of SMA is martensite. Because of the biocompatibility and the corrosion resistance, NiTi shape memory alloy has been widely used in biomedical and aerospace. However, the NiTi binary alloy can only be applied to the ambient temperature, because the phase transformation temperature is lower than 100 centigrade. In order to apply the NiTi based shape memory alloy in the wider field, it is necessary to control the phase transformation temperature, thermal hysteresis latent heat of phase change and other phase transformation behavior and mechanical property effectively and accurately. Thus, it is important to add a third or fourth element to NiTi alloy to form a new type of NiTi based shape memory alloy. The results show that NiTi alloy contains many precipitates such as NiTi2, Ni3Ti2, Ni4Ti3. Adding the third or even the fourth element to the NiTi binary alloy can effectively adjust the phase transition and related mechanical properties of NiTi alloy. In this paper, the research progress of NiTi binary alloys is summarized, the microstructure, types and structures of properties, types and properties of phase transitions of NiTi alloys are introduced respectively, the problems faced by NiTi alloys are analyzed and their prospects are prospected, so as to provide reference for the development of shape memory alloys with better properties. © 2020 Institute of Physics Publishing. All rights reserved.

关键词： Binary alloys

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