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Toward the generation of pure coral genomes with experimental and bioinformatic improvements

The Innovation 2024年第4期5卷 30-32页

作者： Yisi Hu Zhiwei zhang Shuyan Sun Youfang Sun Hui Huang Wenliang Zhou fuwen Wei Center for Evolution and Conservation Biology Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)Guangzhou 511458China Jiangxi Provincial Key Laboratory of Conservation Biology College of ForestryJiangxi Agricultural UniversityNanchang 330029China Key Laboratory of Animal Ecology and Conservation Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China

Stony corals,the primary architects of coral reef ecosystems,are largely underrepresented in omics studies despite their *** presence of endosymbiotic Symbiodiniaceae algae complicates the extraction of pure coral DNA,posing a challenge for genomic ***,we devised a comprehensive methodological framework that incorporates various experimental treatments to achieve 99%purity in coral DNA extraction and a robust bioinformatics pipeline to guarantee the assembly of high-quality,contamination-free coral *** of our framework using Acropora millepora samples demonstrated its efficacy and superiority in obtaining high-quality pure coral genomes using easily accessible adult *** integrated framework serves as a critical foundation for largescale genome-enabled research on stony corals,providing insight into coral evolution and conservation.

关键词： coral guarantee purity

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Ternary polythiophene enables over 17% efficiency organic solar cells

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JOURNAL OF MATERIALS CHEMISTRY A 2024年第18期12卷 10984-10990页

作者： Ai, Qi Lin, Zhihui Wu, Xiangxi Zhu, Yufan Wang, Ke Li, Xiaojun zhang, Jianqi He, Dan Li, Yongfang Zhao, fuwen Cent South Univ Coll Chem & Chem Engn State Key Lab Powder Met Changsha 410083 Peoples R China Chinese Acad Sci Beijing Natl Lab Mol Sci CAS Key Lab Organ Solids Inst Chem Beijing 100190 Peoples R China Natl Ctr Nanosci & Technol CAS Ctr Excellence Nanosci CAS Key Lab Nanosyst & Hierarch Fabricat Beijing 100190 Peoples R China

Polythiophenes (PTs) are one of the most promising donor materials for organic solar cells (OSCs), because of their low-cost production. However, most PT-based OSCs suffer from inferior power conversion efficiencies (PCEs) currently, mainly due to the mismatched energy levels and hyper-miscibility of PTs with state-of-the-art fused ring electron acceptors (FREAs). On account of the issues, two ternary PT donors, PT-CN and PT-2CN, are developed by inserting the simple and strong electron-withdrawing units (3-cyanothiophene and 3,4-dicyanothiophene) into a low-cost PT, PTVT-T, via random copolymerization, respectively. Introducing the ternary unit not only deepens the highest occupied molecular orbital energy level and brings down the miscibility of PTs with FREAs, but also makes the crystallinity of the PTs first increase then decrease from PTVT-T, PT-CN to PT-2CN. Thus, the moderate miscibility and highest crystallinity of PT-CN endows the PT-CN:BTP-eC9 blend film with proper phase separation having orderly molecular stacking to achieve the most efficient exciton dissociation and lowest trap density. Such enables PT-CN:BTP-eC9 based devices to achieve the highest charge carrier mobilities and lifetime, suppressing charge carrier recombination and facilitating charge carrier extraction. Therefore, the PT-CN:BTP-eC9 based OSC affords a higher PCE of 17.27% than PTVT-T:BTP-eC9 and PT-2CN:BTP-eC9 based counterparts, which, to the best of our knowledge, is the highest value for PT-based OSCs so far. The results demonstrate that ternary copolymerization is a facile and efficient route to realize high-efficiency but low-cost PT donors. Two ternary polythiophenes (PTs), PT-CN and PT-2CN, as donors are developed to deepen energy levels, reduce miscibility and regulate crystallinity. PT-CN:BTP-eC9 based OSCs gain an impressive PCE of 17.27%, which is the record for PT-based OSCs.

关键词： Molecular orbitals

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Efficacy and safety of umbilical therapy with the traditional Chinese medicine formulation Lishui Xiaogu cataplasm for cirrhotic ascites: protocol for a randomized controlled trial

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TRIALS 2019年第1期20卷 127-127页

作者： Yang, Xianzhao Ru, Shuying Luo, Lin Lv, Xiaoying Bai, Wenjing zhang, fuwen Jiang, Feng Beijing Univ Chinese Med Dongzhimen Hosp BUCM Inst Liver Dis 5 Haiyuncang Beijing 100700 Peoples R China Beijing Univ Chinese Med Dongzhimen Hosp Eastern Dept Gastroenterol Beijing 101100 Peoples R China China Acad Chinese Med Sci Inst Basic Res Clin Med Beijing 100700 Peoples R China

Ascites is one of the most common complications of cirrhosis. Umbilical therapy with traditional Chinese medicines has been increasingly prescribed to treat cirrhotic ascites. However, high-quality evidence from clinical trials supporting such application of traditional Chinese medicines remains limited. Therefore, we designed a clinical trial to evaluate the efficacy and safety of umbilical therapy with the Lishui Xiaogu cataplasm formulation applied to treat cirrhotic ascites. This ongoing study is a double-blind, randomized, parallel, placebo-controlled trial. A total of 82 patients will be recruited and randomly assigned to either a treatment group or a placebo group, in a 1:1 ratio. The treatment group will receive umbilical therapy with the Lishui Xiaogu cataplasm plus red light irradiation along with conventional treatment;the placebo group will receive umbilical therapy with a placebo cataplasm plus red light irradiation along with conventional treatment. Interventions for both groups will be administered once daily for up to 10 days, with a 30-day follow-up after the last treatment. The primary efficacy measurement will be ascites depth. Secondary efficacy measurements will include abdominal perimeter, weight, urine volume, the symptomatic score of traditional Chinese medicine, and the Chronic Liver Disease Questionnaire. Adverse events will also be reported. This randomized trial will be the first rigorous study designed to evaluate the efficacy and safety of umbilical therapy with Lishui Xiaogu cataplasm applied for cirrhotic ascites. Chinese Clinical Trial Registry, . Registered on 1 January 2016.

关键词： Cirrhotic ascites Lishui Xiaogu cataplasm Traditional Chinese medicine Umbilical therapy Randomized controlled trial

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Spatial-temporal population dynamics of the critically endangered Blue-crowned Laughingthrush Pterorhinus courtoisi:a 22-year longitudinal study

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Science China(Life Sciences) 2024年第8期67卷 1769-1771页

作者： Tao Liu Jutao He Chang Xiao Jie Liu Jie Sun Minling Li Yuanhua Hong Weiwei zhang fuwen Wei Jiangxi Provincial Key Laboratory of Conservation Biology Jiangxi Agricultural UniversityNanchang 330045China Forestry Bureau of Wuyuan Shangrao 333299China

Dear Editor,Population size is a crucial indicator in evaluating the conservation status of a *** the patterns of population fluctuation and the distribution range of endangered species is important for biodiversity c... 详细信息

关键词： Population crown dynamics

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Compacting Molecular Stacking and Inhibiting Self-Aggregation in Fullerene Transporting Layer for Efficient and Stable Perovskite Solar Cells

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ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2025年第22期64卷 e202502950页

作者： He, Dan zhang, Jiahao Gong, Xue-Yuan Ruan, Xinying Ma, Xin-Bo Yao, Chaoyi Shen, Xingxing Li, Ming-Hua zhang, Jianqi Hu, Jin-Song Wang, Chunru Zhao, fuwen Cent South Univ Coll Chem & Chem Engn State Key Lab Powder Met Changsha 410083 Peoples R China Beijing Univ Chem Technol Coll Chem Engn Beijing 100029 Peoples R China Chinese Acad Sci Inst Chem Beijing Natl Lab Mol Sci CAS Key Lab Mol Nanostruct & Nanotechnol Beijing 100190 Peoples R China Hebei Normal Univ Sci & Technol Coll Chem Engn Qinhuangdao 066004 Peoples R China Natl Ctr Nanosci & Technol CAS Ctr Excellence Nanosci CAS Key Lab Nanosyst & Hierarch Fabricat Beijing 100190 Peoples R China

The underdevelopment of electron transport layer (ETL) materials remains a critical bottleneck limiting the overall photovoltaic performance of inverted perovskite solar cells (PSCs). Fullerene derivatives, such as PCBM, are widely employed ETL materials in PSCs due to their excellent electron affinity and energy level alignment with the perovskite layer. However, PCBM suffers from high energy disorder, self-aggregation predilection, and insufficient defect passivation ability, leading to significant charge carrier recombination and accumulation at interfaces. Herein, a phosphate-substituted fullerene derivative, FuPE, is developed to enhance the performance of PCBM-based ETLs for PSCs. Incorporating FuPE efficiently compacts molecular stacking, enforces crystallinity and intermolecular interaction, suppresses self-aggregation, and improves interfacial compatibility of the FuPE:PCBM blend. Such endows the FuPE:PCBM blend film with enhanced electron mobility (0.183 cm2 V-1 s-1), lower trap density, more uniform film morphology, and superior defect-passivation ability, compared to the PCBM pristine one. Consequently, PSCs employing FuPE:PCBM as the ETL achieve reduced trap-assisted recombination, enhanced charge carrier extraction, and thus a remarkable power conversion efficiency exceeding 26% alongside improved operational stability. This work highlights an effective strategy for optimizing fullerene-based ETLs, advancing the development of highly efficient and durable PSCs.

关键词： Charge carrier transport Electron transport layer Fullerene materials Interfacial passivation Perovskite solar cells

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铸钢工程车桥壳消失模铸造

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铸造技术 2020年第3期41卷 283-285,290页

作者：张复文童容英栾新华分宜九一机械制造有限公司江西新余336600

基于消失模铸造工艺特点,结合铸钢重载工程车桥壳的特殊技术要求,采用消失模铸造工艺生产了重载工程车桥壳。结果表明,采用改进铸造工艺、STMMA珠粒白模+烧空浇注、防裂环、底漏钢包+吹氩气浇注、框架支撑+精细操作等一系列有效措施,成... 详细信息

基于消失模铸造工艺特点,结合铸钢重载工程车桥壳的特殊技术要求,采用消失模铸造工艺生产了重载工程车桥壳。结果表明,采用改进铸造工艺、STMMA珠粒白模+烧空浇注、防裂环、底漏钢包+吹氩气浇注、框架支撑+精细操作等一系列有效措施,成功生产了重载工程车桥壳,解决了增碳,开裂,夹杂渗漏,变形等缺陷。同时,浇注过程中的高频振动细化了铸件晶粒。

关键词： STMMA 消失模铸造桥壳底漏钢包

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Reasoning in Conversation: Solving Subjective Tasks through Dialogue Simulation for Large Language Models 62

Reasoning in Conversation: Solving Subjective Tasks through ...

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62nd Annual Meeting of the Association-for-Computational-Linguistics (ACL) / Student Research Workshop (SRW)

作者： Wang, Xiaolong Wang, Yile zhang, Yuanchi Luo, fuwen Li, Peng Sun, Maosong Liu, Yang Tsinghua Univ Inst AI Dept Comp Sci & Tech Beijing Peoples R China Tsinghua Univ Inst AI Ind Res AIR Beijing Peoples R China Jiangsu Collaborat Innovat Ctr Language Competenc Nanjing Jiangsu Peoples R China Jiuquan Satellite Launch Ctr JSLC Jiuquan Gansu Peoples R China

ISBN: (纸本)9798891760943

Large Language Models (LLMs) have achieved remarkable performance in objective tasks such as open-domain question answering and mathematical reasoning, which can often be solved through recalling learned factual knowledge or chain-of-thought style reasoning. However, we find that the performance of LLMs in subjective tasks is still unsatisfactory, such as metaphor recognition, dark humor detection, etc. Compared to objective tasks, subjective tasks focus more on interpretation or emotional response rather than a universally accepted reasoning pathway. Based on the characteristics of the tasks and the strong dialogue-generation capabilities of LLMs, we propose RiC (Reasoning in Conversation), a method that focuses on solving subjective tasks through dialogue simulation. The motivation of RiC is to mine useful contextual information by simulating dialogues instead of supplying chain-of-thought style rationales, thereby offering potential useful knowledge behind dialogues for giving the final answers. We evaluate both API-based and open-source LLMs including GPT-4, ChatGPT, and OpenChat across twelve tasks. Experimental results show that RiC can yield significant improvement compared with various baselines.

关键词： Computational linguistics

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氧化应激在青光眼发病中的作用及天然药物治疗新进展

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中国中医眼科杂志 2020年第11期30卷 814-817页

作者：向圣锦张富文段俊国成都中医药大学成都610075 温州医科大学附属眼视光医院温州325027

氧化应激是机体内氧自由基的产生与清除失衡,导致细胞、组织损伤的状态。氧化物与抗氧化物水平的改变和氧化损伤标记物的大量出现,提示氧化应激可能在青光眼损害中发挥着重要的作用。活性氧可以通过损伤小梁网、视网膜、视乳头而导致眼... 详细信息

氧化应激是机体内氧自由基的产生与清除失衡,导致细胞、组织损伤的状态。氧化物与抗氧化物水平的改变和氧化损伤标记物的大量出现,提示氧化应激可能在青光眼损害中发挥着重要的作用。活性氧可以通过损伤小梁网、视网膜、视乳头而导致眼压升高,引起组织氧化应激反应,最终导致视网膜神经节细胞凋亡以及不可逆的视功能损害。同时,抗氧化损伤的天然药物对抑制青光眼视网膜神经节细胞凋亡显示出一定的疗效。本文总结了氧化应激在青光眼发病中的作用以及抗氧化应激天然药物防治青光眼视神经损害的研究进展,以期为进一步阐明青光眼的发病机制及其治疗提供更好的参考。

关键词：青光眼氧化应激标志物小梁网视网膜神经节细胞天然药物

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Structure changes of lignin and their effects on enzymatic hydrolysis for bioethanol production: a focus on lignin modification

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JOURNAL OF BIOTECHNOLOGY 2024年 393卷 61-73页

作者： Hou, Jinju zhang, Qiuzhuo Tian, Fuxiang Liu, fuwen Jiang, Jingxian Qin, Jiaolong Wang, Huifeng Wang, Jing Chang, Shufang Hu, Xiaojun Shanghai Inst Technol Sch Chem & Environm Engn Shanghai 201418 Peoples R China East China Normal Univ Shanghai Engn Res Ctr Biotransformat Organ Solid W Sch Ecol & Environm Sci Shanghai Key Lab Urban Ecol Proc & Ecorestorat Shanghai 200241 Peoples R China Shanghai Inst Technol Sch Mat Sci & Engn Shanghai 201418 Peoples R China

Enzymatic hydrolysis contributes to obtaining fermentable sugars using pretreated lignocellulose materials for bioethanol generation. Unfortunately, the pretreatment of lignocellulose causes low substrate enzymatic hydrolysis, which is due to the structure changes of lignin to produce main phenolic by-products and nonproductive cellulase adsorption. It is reported that modified lignin enhances the speed of enzymatic hydrolysis through single means to decrease the negative effects of fermentation inhibitors or non-productive cellulase adsorption. However, a suitable modified lignin should be selected to simultaneously reduce the fermentation inhibitors concentration and non-productive cellulase adsorption for saving resources and maximizing the enzymatic hydrolysis productivity. Meanwhile, the adsorption micro-mechanisms of modified lignin with fermentation inhibitors and cellulase remain elusive. In this review, different pretreatment effects toward lignin structure, and their impacts on subsequent enzymatic hydrolysis are analyzed. The main modification methods for lignin are presented. Density functional theory is used to screen suitable modification methods for the simultaneous reduction of fermentation inhibitors and non-productive cellulase adsorption. Lignin-fermentation inhibitors and lignin-cellulase interaction mechanisms are discussed using different advanced analysis techniques. This article addresses the gap in previous reviews concerning the application of modified lignin in the enhancement of bioethanol production. For the first time, based on existing studies, this work posits the hypothesis of applying theoretical simulations to screen efficient modified lignin-based adsorbents, in order to achieve a dual optimization of the detoxification and saccharification processes. We aim to improve the integrated lignocellulose transformation procedure for the effective generation of cleaner bioethanol.

关键词： Bioethanol Pretreatment Modified lignin Adsorption mechanism

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Based on the combination of fluid-solid interaction mechanism model and surrogate model for peristaltic pump performance analysis and multi-objective optimization design

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ADVANCED ENGINEERING INFORMATICS 2024年 62卷

作者： Liu, fuwen Gong, Zhuangzhuang Ma, Xinao zhang, Yanfeng Song, Xueguan Dalian Univ Technol Sch Mech Engn Dalian 116023 Peoples R China Dalian Univ Technol State Key Lab High Performance Precis Mfg Dalian 116023 Peoples R China Baoding Lead Fluid Technol Co Ltd Baoding 071000 Peoples R China Hebei Prov Fluid Precis Transmission Technol Innov Sanhe Peoples R China

The peristaltic pump is a high precision biomimetic pump widely used in the medical industry. However, the pump has flow pulsation, due to its special structure. The serious flow pulsation can dramatically reduce delivery accuracy and stability. Meanwhile, the service life of the peristaltic pump is affected by the conveying capacity. The flow pulsation degree and transportation capacity of the pump are affected by the structural parameters of its key components. An in-depth study of peristaltic pumps is necessary in order to reduce the degree of pulsation and increase service life. The paper skillfully combines the fluid-solid interaction (FSI) mechanism model with the surrogate model to deeply study the influence of key structural parameters of the peristaltic pump on its performance, and the multi-objective optimization design of the pump is carried out by using the multi-island genetic algorithm (MIGA). The results show that for the average flow, the hose inner diameter has the greatest influence, followed by the working circle diameter. The roller diameter has very little effect. The hose inner diameter and working circle diameter have a minor coupling effect on the roller diameter. It slightly affects the trend of the average flow rate with the roller diameter. The hose inner diameter also has a slight coupling effect on the working circle diameter. For the instantaneous flow rate percentage, the effects of all three key parameters are significant. The hose inner diameter has an obvious coupling effect on the working circle diameter and the roller diameter, which affects the trend of the instantaneous flow rate percentage with the working circle diameter and the roller diameter. The optimized pump has a 43.3% decrease in the percentage of flow rate and a 59.6% increase in the average flow rate, indicating significant improvements in transport stability and capacity.

关键词： Fluid -solid interaction MIGA Multi -objective optimization Peristaltic pump Surrogate model

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