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色谱 2010年第1期28卷 34-37页

作者：罗福文陶定银赵鹏张凌怡贾玉杰张维冰大连医科大学病理生理教研室辽宁大连116027 大连医科大学附属第二医院普通外科辽宁大连116027 中国科学院大连化学物理研究所中国科学院分离分析化学重点实验室国家色谱分析研究中心辽宁大连116023 华东理工大学化学与分子工程学院上海200237

通过蛋白质组学技术筛选胃癌相关标志物是目前胃癌研究的热点,也是早期诊断的关键。针对组织蛋白质提取物非常复杂的特点,并根据疏水性的差异,采用反相液相色谱对正常及癌症组织提取蛋白质进行分离。通过比较正常及癌症组织提取蛋白质... 详细信息

通过蛋白质组学技术筛选胃癌相关标志物是目前胃癌研究的热点,也是早期诊断的关键。针对组织蛋白质提取物非常复杂的特点,并根据疏水性的差异,采用反相液相色谱对正常及癌症组织提取蛋白质进行分离。通过比较正常及癌症组织提取蛋白质的谱图差异,收集并酶解差异最大的保留时间为45～47min的馏分,采用液相色谱-多级质谱联用(LC-MS/MS)鉴定其酶解产物。鉴定结果显示,正常及癌症组织中的共有蛋白质为9个,正常组织中有6个特异蛋白质,而癌症组织中有17个特异蛋白质。通过进一步分析,筛选出胃癌组织中含有的丰度较高的两个蛋白质。应用生物信息学方法分析这些蛋白质,能为将来的药物靶点、药物作用通路研究提供更多的信息。

关键词：液相色谱-多级质谱法特异蛋白质癌症胃组织

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THE EFFECT OF QIDENGMINGMU CAPSULE ON THE VASCULAR ENDOTHELIAL GROWTH FACTOR IN VITREOUS AND PROTEIN KINASE C IN RETINAS IN SPONTANEOUS DIABETES RAT

THE EFFECT OF QIDENGMINGMU CAPSULE ON THE VASCULAR ENDOTHELI...

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第十八届国际眼科研究大会

作者： Qiang Li Junguo Dnan fuwen zhang Eye Laboratory of Chengdu University of Traditional Chinese Medicine 610075

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What determines selection and abandonment of a foraging patch by wild giant pandas (Ailuropoda melanoleuca) in winter?

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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 2009年第1期16卷 79-84页

作者： zhang, Zejun Zhan, Xiangjiang Yan, Li Li, Ming Hu, Jinchu Wei, fuwen Chinese Acad Sci Key Lab Anim Ecol & Conservat Biol Beijing 100101 Peoples R China China W Normal Univ Inst Rare Anim & Plants Nanchong 637002 Sichuan Peoples R China

Foraging patches can be described as a nested hierarchy of aggregated resources, implying that study of foraging by wild animals should be directed across different spatial scales. However, almost all previous research on habitat selection by the giant panda has concentrated upon one scale. In this research, we carried out a field study to understand foraging patch selection by giant pandas in winter at both microhabitat and feeding site scales and, for the first time, attempted to understand how long it would stay at the feeding sites before moving on. The field survey was conducted from November 2002 to March 2003 at Fengtongzhai Nature Reserve (102A degrees 48'aEuro"103A degrees 00' E, 30A degrees 19'aEuro"30A degrees 47' N), Baoxing County of Sichuan Province, China, to collect data in both microhabitat and control plots. The microhabitat plots were located by fresh feces or foraging traces left by giant pandas, and the control plots were established to reflect the environment. Within each microhabitat plot, one 1 x 1 m(2) plot was centralized at the center of each feeding site, in which numbers of old bamboos and old shoots, including eaten and uneaten, were counted, respectively. The results showed that winter microhabitats selected by this species were characteristic of gentle slopes and high old-shoot proportions and that the latter was even higher at feeding sites. Two selection processes, namely, from the environment to microhabitats and from the latter to feeding sites, were found during this species' foraging patch utilization. Giant pandas preferred to eat old shoots to old bamboo at feeding sites in winter and did not leave unless old-shoot density fell to lower than the average in the environment. Both microhabitats and feeding sites selected by giant pandas were characteristic of high old-shoot density, indicating that the preferred food item had a significant influence upon its foraging patch selection. The preference for gentle slopes by giant pand

关键词： Feeding site Fengtongzhai Nature Reserve Giant panda (Ailuropoda melanoleuca) Giving-up density Marginal value theorem Microhabitat Old shoot Winter

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Food habits and space-use of red pandas Ailurus fulgens in the Fengtongzhai Nature Reserve, China: food effects and behavioural responses

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ACTA THERIOLOGICA 2009年第3期54卷 225-234页

作者： zhang, Zejun Hu, Jinchu Yang, Jiandong Li, Ming Wei, fuwen Chinese Acad Sci Key Lab Anim Ecol & Conservat Biol Inst Zool Beijing 100101 Peoples R China China W Normal Univ Inst Rare Anim & Plants Nanchong 637002 Sichuan Peoples R China

The red panda Ailurus fulgens Cuvier, 1825 is endemic to the Himalayan-Hengduan Mountains. Many of its ecological traits are still poorly understood although important from a scientific standpoint as a highly specialized bamboo feeder. To understand its food habits and space-use, we carried out a field study from April 2002 to May 2003 in Fengtongzhai Nature Reserve, China. The results showed that red pandas almost exclusively fed on Bashania faberi, but not on Yushania brevipaniculata in the study area. Besides differences in nutritional quality, accessibility to food items is perhaps another factor affecting their utilization of bamboo species. B. faberi leaves are the highest in crude protein, being the primary year-round food source;new shoots and fruits of Sorbus, and Rubus are nutritious and digestible, seasonally occurred in their diet. Red pandas seemed to select most nutritious food items to feed on. The home range of red pandas averaged 2.2 km(2) across individuals (2.6 km(2) for the males and 1.7 km(2) for the females), and overlapped extensively (ranging 18.9-78.1%). No significant difference was found in monthly percentage of total home range used among seasons and sexes. The daily movement distance was 455 m (447 m for the females and 463 m for the males), significantly affected by sexes and months. Our findings indicated that food resource significantly influenced behaviour of red pandas in foraging and space use, and their behavioural responses are adaptive being helpful to energy intake and maintenance.

关键词： Ailurus fulgens food habits home range daily movement Bashania faberi Yushania brevipaniculata

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Fault Detection for Descriptor Networked Systems with Multiple Packet Dropouts

Fault Detection for Descriptor Networked Systems with Multip...

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Joint 48th IEEE Conference on Decision and Control (CDC) / 28th Chinese Control Conference (CCC)

作者： Wang, Jia Wang, Wu Yang, fuwen zhang, Jinxian East China Univ Sci & Technol Sch Informat Sci & Engn Shanghai 200237 Peoples R China Fuzhou Univ Coll Elect Engn & Automat Fuzhou 350108 Fujian Peoples R China Fujian Key Lab Med Instrumentat & Pharmaceut Tech Fuzhou 350002 Peoples R China

ISBN: (纸本)9781424438723

Based on a regular, causal descriptor controlled plant, the fault detection for a class of descriptor systems with both multiple measurement and control packet dropouts is investigated. Two independent Bernoulli distributed white sequences are used to describe the multiple measurement and control packet dropouts. The problem of fault detection for networked systems can be formulated as an H infinity filtering problem in terms of H infinity theory. Then a fault detection filter which has a given H infinity performance is designed. A sufficient condition for the existence of the filter is established by means of linear matrix inequalities. At last a numerical example shows the validity of the proposed method.

关键词： Fault detection

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Sm-Nd, Pb-Pb and Rb-Sr Isotopic Dating and Its Dynamic Implications for the Proterozoic Augen Granite in the Yunkai Area, Western Guangdong Province

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Acta Geologica Sinica - English Edition 2010年第2期74卷

作者： PENG Songbai zhang Yeming ZHAN Mingguo CHEN fuwen QIU Ruizhao Yichang Institute of Geology and Mineral Resources CAGS Yichang 443003 Hubei

The samples of ductile-rheologic deformational augen granite from the Yunkai uplift area, western Guangdong province, were determined by the whole-rock Sm-Nd, Pb-Pb and Rb-Sr isotopic dating to have an Sm-Nd isochron age of 1414±68 Ma, a Pb-Pb isochron age of 1388±90 Ma and a Rb-Sr isochron age of 490±36 Ma. The first two ages are interpreted as the formation age of this suite of granite and the last age represents the timing of the tectono-thermal event of Caledonian ductile-rheologic shear partial melting. It is indicated that in the study area not only an orogeny took place in the Caledonian, but also a more important tectono-magmatic activity occurred in the Meso-proterozoic there, which may be related to the subduction-collision between the Yangtze block and Cathaysia block.

关键词： Yunkai area Proterozoic augen granite isotopic dating ductile-rheologic shear

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秦岭巴山木竹微量元素及营养成分分析

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兽类学报 2010年第2期30卷 223-228页

作者：孙宜然张泽钧李林辉刘新玉魏辅文马亦生韦伟廖钫何少文西华师范大学珍稀动植物研究所中国科学院动物研究所佛坪国家级自然保护区

Being widely distributed in the Qinling Mountains,Bashania fargesii is the primary food source for giant pandas (Ailuropoda melanoleuca) during winter and *** understand the relationship between panda food habits and nutrients in the bamboo,we collected samples of leaves,branches and stems across seasons and ages in Foping Nature Reserve,Shaanxi Province,China. Each category of samples was collected at the same site,with three replicates,and weighing 200 to 500 gram. Seven trace elements (Zn,Cu,Fe,Mn,Ca,Mg,K) were analyzed by the atomic absorption spectrophometric method. Crude protein was determined following Macro-Kjelhahl procudure,and crude fat was measured with Soxhlet procedure. The results indicated that contents of Mn,Ca,Mg in the leaves and Cu,Zn,Fe in the branches were respectively higher than those in the other parts on bamboo. Compared with those in the branches and stems,contents of crude protein and crude fat in the leaves were significantly higher. In addition,contents of trace elements and nutrients in the bamboos varied across seasons and ages. Leaves are the most nutritious among different parts of bamboo. Food habits exhibited by giant pandas were closely related to nutrition quality of leaves in the bamboo.

关键词：大熊猫巴山木竹微量元素营养成分食性

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Free-weighting matrices approach to H∞ filtering for a class of uncertain linear systems with time-varying delay

Free-weighting matrices approach to H∞ filtering for a clas...

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2009 Chinese Control and Decision Conference, CCDC 2009

作者： Jinxian, zhang Wu, Wang Fujun, Cui fuwen, Yang College of Electrical Engineering and Automation Fuzhou University Fuzhou 350108 China Fujian Key Laboratory of Medical Instrumentation and Pharmaceutical Technology Fuzhou 350002 China School of Information Science and Engineering East China University of Science and Technology Shanghai 200237 China

ISBN: (纸本)9781424427239

This paper investigates the problem of delay-dependent robust H ∞ filtering for uncertain linear systems with time-varying delay. Given a stable system with parameter uncertainties residing in a polytope with s vertices, the focus is on designing a robust H∞ filter such that the filtering error system is robustly asymptotically stable with a prescribed H∞ noise attenuation level. The main technique used is the free-weighting matrix method by considering the additional useful terms. As a result, an improved linear matrix inequality-based delay-dependent stability criterion is obtained, which is less conservative. Finally, two examples have been presented to illustrate the effectiveness of the proposed methodology. © 2009 IEEE.

关键词： Uncertain systems

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Accurate population size estimates are vital parameters for conserving the giant panda

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URSUS 2009年第1期20卷 56-62页

作者： Zhan Xiangjiang Tao Yi Li Ming zhang Zejun Goossens, Benoit Chen Youping Wang Hongjia Bruford, Michael Wei fuwen Chinese Acad Sci Key Lab Anim Ecol & Conservat Biol Inst Zool Beijing 100101 Peoples R China Cardiff Univ Biodivers & Ecol Proc Grp Cardiff Sch Biosci Cardiff CF10 3AX S Glam Wales Wanglang Nat Reserve Pingwu Cty 622550 Sichuan Peoples R China Sichuan Forestry Dept Wildlife Conservat Div Chengdu 610081 Sichuan Peoples R China

Although the Chinese government and international organizations have spent millions of dollars estimating the population size of giant pandas (Ailuropoda melanoleuca), the outcomes of such surveys have been debated because the methods used had uncertain ability to differentiate individuals. Our work in Wanglang Reserve was the first attempt at using fecal microsatellite analysis to enumerate giant pandas (Zhan et al. 2006), and we found the molecular enumeration for the reserve more than doubled that estimated in the third National Survey. Recently, Garshelis et al. (2008) challenged our DNA estimate on the grounds that our study lacked geographic closure and that genotyping errors confounded our results. We challenge both assertions. First, we realize the violation of the assumption of geographic closure is a common problem for noninvasive genetic capture-recapture studies;therefore, we tried in our methodology to minimize geographic closure effects. We conclude that immigration did not greatly affect our main conclusions because of a geographic barrier circling the study area, the limited mobility of giant pandas, and the same sampling intensity was applied to the whole study area. Second, internationally accepted standards were applied to our molecular analysis and appropriate laboratory procedures were taken to produce reliable genotypes. Although we did not report an estimate of genotyping error in our previous work (Zhan et al. 2006), genotyping error rates have now been estimated, but are still pending publication. Based on the Wanglang result, we raised the possibility that there might be more giant pandas in the wild than previously thought. If our prediction is shown to be correct, we do not believe the new estimate will serve to downgrade current endangered status of the giant panda, because population numbers remain very low and panda habitats are fragmented (even more so since the 2008 earthquake). For future conservation of giant pandas, we suggest mor

关键词： Ailuropoda melanoleuca bamboo stem genotyping error giant panda molecular enumeration population geographic closure population size

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THE EFFECT OF QIDENGMINGMU CAPSULE ON THE VASCULAR ENDOTHELIAL GROWTH FACTOR IN VITREOUS AND PROTEIN KINASE C IN RETINAS IN SPONTANEOUS DIABETES RAT

THE EFFECT OF QIDENGMINGMU CAPSULE ON THE VASCULAR ENDOTHELI...

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第十八届国际眼科研究大会

作者： Qiang Li Junguo Duan fuwen zhang Eye Laboratory of Chengdu University of Traditional Chinese Medicine 610075

Objective:To observe the effect of QiDengMingMu capsule on protein kinase C in retinas and the Vascular endothelial growth factor in vitreous of the experimental animal model of spontaneous diabetic retinopathy rats (... 详细信息

关键词： PKC THE EFFECT OF QIDENGMINGMU CAPSULE ON THE VASCULAR ENDOTHELIAL GROWTH FACTOR IN VITREOUS AND PROTEIN KINASE C IN RETINAS IN SPONTANEOUS DIABETES RAT

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