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Detection of Porcine Norovirus GII.18 Strains in Pigs Using Broadly Reactive RT-qPCR Assay for Genogroup II Noroviruses

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FOOD AND ENVIRONMENTAL VIROLOGY 2025年第1期17卷 1-8页

作者： gupta, ankita k. Heinonen, Mari k.nig, Emilia Mik.onen, Venla Maunula, Leena Univ Helsinki Fac Vet Med Dept Food Hyg & Environm Hlth Helsinki Finland Univ Helsinki Fac Vet Med Dept Prod Anim Med Helsinki Finland

Noroviruses, belonging to the family Caliciviridae, are classified into at least ten genogroups (G) based on their major capsid protein (VP1). The common genogroup to be identified in both humans and pigs is GII, although porcine noroviruses (PoNoVs) belong to genotypes of their own (GII.11, GII.18, and GII.19). So far, PoNoVs have not been studied much in Finland, possibly due to their rather symptomless nature in pigs. In the present study, we enrolled a total of 189 fecal samples collected from pigs from Finnish farms. Samples were tak.n from 12 farms in 2010, 2019 and 2020. We analyzed feces from growing pigs ranging from 2.1 to 6 months of age. RNA was extracted from fecal suspensions using a commercial viral RNA extraction k.t, followed by RT (reverse transcription)-qPCR. The genotypes were determined by Sanger sequencing of the PCR fragments amplified by conventional PCR. Of the 12 farms, 6 (50%) had at least one PoNoV-infected pig. Altogether 18 (9.5%) of the 189 pigs tested positive for PoNoVs. Pigs mostly aged over 4 months were infected with PoNoVs. Eventually, 12 positive samples were determined as genotype GII.18. We could demonstrate the presence of PoNoVs in Finnish pigs. In future, more studies in which longer sequences from PoNoV genome can be obtained, are required.

关键词： Caliciviridae Human norovirus Porcine norovirus RT-qPCR Genotyping

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Emerging evidence of artemin/GFRα3 signaling in musculosk.letal pain

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OSTEOARTHRITIS AND CARTILAGE 2025年第2期33卷 196-206页

作者： gupta, ankita Mishra, Santosh k. Lascelles, B. Duncan X. North Carolina State Univ Coll Vet Med Dept Clin Sci Translat Res Pain Program Raleigh NC USA North Carolina State Univ Coll Vet Med Dept Mol Biomed Sci Raleigh NC 27695 USA North Carolina State Univ Comparat Pain Res & Educ Ctr Raleigh NC 27695 USA UNC Sch Med Thurston Arthrit Ctr Chapel Hill NC USA Duke Univ Ctr Translat Pain Res Dept Anesthesiol Durham NC USA

Chronic musculosk.letal pain is highly prevalent and poses a significant personal, societal, and economic burden. Management of chronic musculosk.letal pain remains a challenge. Long-term use of common analgesic medications such as nonsteroidal anti-inflammatory drugs and opioids is associated with adverse events, and in the case of opioids, drug addiction. Additionally, many individuals do not experience sufficient pain relief with these therapeutic approaches. Thus, there is an urgent need to develop clinically efficacious and safe therapeutics for musculosk.letal pain. Recent advances in our understanding of musculosk.letal pain neurobiology have helped identify the role of neurotrophic factors, specifically, the glial cell line-derived neurotrophic factor (GDNF) family of ligands (GFL) and their associated signaling pathways. This review outlines our current understanding of the GFL signaling systems, discusses their role in inflammatory and chronic musculosk.letal pain and sensitivity, and comments on the analgesic therapeutic potential of targeting the GFL signaling system. (c) 2024 The Authors. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International. This is an open access article under the CC BY-NC-ND license (http://***/licenses/by-ncnd/4.0/).

关键词： GDNF family of ligands Artemin GFR alpha 3 Musculosk.letal Pain

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Engineering the decay time of Ti3C2Tx MXene by gold nanoparticle decoration

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NANOTECHNOLOGY 2025年第15期36卷 155702-155702页

作者： Rajput, Gaurav Rawat, ankita Chourasia, Nitesh k. Jalendra, Gaurav gupta, Govind Yadav, Aditya k.lriya, P. k. Jawaharlal Nehru Univ Sch Phys Sci New Delhi 110067 India Pandit Deendayal Energy Univ Sch Energy Technol Dept Phys Gandhinagar 382426 Gujarat India Univ Delhi Dept Phys & Astrophys New Delhi 110007 India CSIR Natl Phys Lab New Delhi India

MXenes, specifically Ti3C2Tx having peculiar structural and electronic characteristics display not only high surface area, and excellent thermal and electrical conductivity but also have the potential for functionalization. The primary focus of this research is to control the decay time of gold nanoparticle (NP) (Au NP) decorated multilayer Ti3C2Tx MXene (Au-Ti3C2Tx) synthesized by a simple two-step selective etching technique. Incorporation of Au NPs in the multilayer Ti3C2Tx MXene leads to lattice expansion, micro-strain reduction, and crystallinity improvement, as confirmed by x-ray diffraction analysis. Observation of a well-developed G band in the Au-Ti3C2Tx MXene across different Au concentrations by Raman spectroscopy investigations suggests the accumulation of graphitic carbon on the MXene surface which has greatly improved the charge transfer characteristic of the carbide layer. Furthermore, the Au-Ti3C2Tx MXene exhibits promising optical properties for different concentrations of gold. The time-resolved photoluminescence spectroscopy studies displayed a reduction in the average decay time (tau av) to similar to 30% with increasing gold concentration from 100 to 150 mu l in Au NPs solution which is explained based on Au NPs induced surface plasmon resonance. The decoration of Au NPs facilitates the accumulation of carbon on the surface of MXene, resulting in enhanced crystallinity, reduced micro-strain, and decreased decay time. By engineering decay time through the decoration of noble metal NPs onto MXene, it becomes possible to fabricate highly efficient photodetectors and imaging devices. This is especially advantageous in applications where shorter decay times are desired.

关键词： MXene gold nanoparticles graphitic carbon photoluminescence

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QbD-Based Development of Fluocinolone Nanocomposite Transdermal Gel: Optimization, Characterization, and Enhanced Anti-hyperpigmentation Efficacy Assessment

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AAPS PHARMSCITECH 2025年第4期26卷 1-26页

作者： Rathore, Priyank. gupta, Rishik.sh Singh, Prem Prak.sh Awasthi, Anshu k.shore, ankita Bansal, k.ldeep k. Mahor, Alok.k.mar Bundelkhand Univ Inst Pharm Jhansi 284128 Uttar Pradesh India Amity Univ Madhya Pradesh Amity Inst Pharm Gwalior 474005 India Abo Akad Univ Fac Sci & Engn Pharmaceut Sci Lab Turku 20520 Finland

The current study presents a comprehensive pharmaceutical engineering approach to developing an advanced transdermal drug delivery system for addressing sk.n hyperpigmentation through innovative nanocomposite gel formulation. Utilizing a systematic Quality-by-Design (QbD) methodology with Box-Behnk.n design, we developed a novel fluocinolone-loaded chitosan-graphene oxide nanocomposite (FCGN1) aimed at optimizing pharmaceutical performance and therapeutic efficacy. The nanocomposite formulation demonstrated critical pharmaceutical quality attributes: a precisely controlled nanoscale particle size of 144.78 +/- 0.15 nm, stable zeta potential of -17.93 +/- 3.75 mV, and high drug entrapment efficiency of 81.3 +/- 3.64%. The optimized gel formulation (FNTG3) exhibited superior transdermal delivery characteristics, achieving approximately 70% permeation within 15 h and a significant flux rate of 190 mu g/cm(2), which substantially outperforms current mark.t alternatives. The comprehensive pharmaceutical evaluation included rigorous stability studies over 45 days, confirming consistent physical stability and sustained drug permeation. In vivo assessments using a UVB-induced hyperpigmentation rat model validated the formulation's dermal tolerability and depigmentation potential, demonstrating comparable or superior performance to commercial hydroquinone treatments. Histopathological analyses revealed pronounced depigmentation effects, attributable to the synergistic design of the nanocomposite system. The strategic integration of fluocinolone, chitosan, and graphene oxide facilitated enhanced drug release k.netics and improved sk.n penetration, highlighting the potential of rational pharmaceutical design in developing advanced topical delivery systems. This research provides a robust framework.for developing sophisticated pharmaceutical dosage forms with enhanced therapeutic performance, offering significant insights into nanoscale drug delivery technologies for dermatolog

关键词： Chitosan Depigmentation activity Fluocinolone Graphene oxide Nanocomposites

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Validation and Recalibration of a Model for Predicting Surgical-Site Infection After Pelvic Organ Prolapse Surgery

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INTERNATIONAL UROGYNECOLOGY JOURNAL 2025年第2期36卷 431-438页

作者： Rhodes, Stephen Sahmoud, Amine Jelovsek. J. Eric Bretschneider, C. Emi gupta, ankita Hijaz, Adonis k. Sheyn, David Univ Hosp Cleveland Urol Inst Div Urogynecol Cleveland OH 44106 USA Univ Hosp Cleveland Dept Obstet & Gynecol Cleveland OH USA Duke Univ Sch Med Dept Obstet & Gynecol Div Urogynecol Durham NC USA Northwestern Univ Dept Obstet & Gynecol Div Urogynecol Chicago IL USA Univ Louisville Dept Obstet & Gynecol Div Urogynecol Louisville KY USA Univ Hosp Cleveland Dept Urol 11100 Euclid Ave Cleveland OH 44106 USA

Introduction and HypothesisThe objective was to externally validate and recalibrate a previously developed model for predicting postoperative surgical-site infection (SSI) after pelvic organ prolapse (POP) *** study utilized a previously validated model for predicting post-POP surgery SSI within 90 days of surgery using a Medicare population. For this study, the model was externally validated and recalibrated using the Premier Healthcare Database (PHD) and the National Surgical Quality Improvement Project (NSQIP) database. Discriminatory performance was assessed via the c-statistic and calibration was assessed using calibration curves. Methods of recalibration in the large and logistic recalibration were used to update the *** PHD contained 420,277 POP procedures meeting the inclusion criteria and 1.6% resulted in SSI. The NSQIP dataset contained 62,553 POP surgeries and 1.4% resulted in SSI. Discrimination of the original model was comparable with that seen in the initial validation (c-statistic = 0.57 in PHD, 0.59 in NSQIP vs 0.60 in the original Medicare data). Recalibration greatly improved model calibration when evaluated in NSQIP *** previously developed model for predicting SSI after POP surgery demonstrated stable discriminatory ability when externally validated on the PHD and NSQIP databases. Model recalibration was necessary to improve prediction. Prospective studies are needed to validate the clinical utility of such a model.

关键词： Pelvic organ prolapse Surgical-site infection Artificial intelligence Prediction model Validation

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International Urogynecological Consultation Chapter 2.2: Imaging in the Diagnosis of Pelvic Organ Prolapse

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INTERNATIONAL UROGYNECOLOGY JOURNAL 2025年第4期36卷 759-781页

作者： Lipetsk.ia, Lioudmila gupta, ankita Cheung, Rachel Y. k. k.ullar, Vik.Ismail, Sharif Bradley, Megan k.rmak.r, Roopali Clifton, Shari Doo, Josephine Quiroz, Lieschen Rowan Univ Dept Obstet & Gynecol Cooper Med Sch Div Urogynecol 402 E Oak Ave Moorestown NJ 08103 USA Univ Louisville Dept Obstet & Gynecol Div Urogynecol Louisville KY USA Chinese Univ Hong Kong CUHK Dept Obstet & Gynaecol Hong Kong Peoples R China Imperial Coll London Dept Urogynaecol London England Univ Hosp Sussex Natl Hlth Serv NHS Fdn Trust Brig Brighton England Univ Pittsburgh Dept Obstet & Gynecol Div Urogynecol Pittsburgh PA USA West Middlesex Univ Hosp Chelsea & Westminster NHS Fdn Trust Dept Obstet & Gynaecol Isleworth England Univ Oklahoma Hlth Sci Ctr Dept Obstet & Gynecol Div FPMRS Oklahoma City OK USA

Introduction and Hypothesis This section of Chapter 2.2 of the International Urogynecology Consultation on Pelvic Organ Prolapse (POP), reviews the literature on the role of imaging in the diagnosis of POP. Methods An international group of nine urogynecologists and one university-based medical librarian adhered to the framework.of the scoping review. The group performed a search of the literature using pre-specified search terms in Scopus, OVID Medline, and PubMed. Publications were eliminated if not relevant to the diagnostic value of POP imaging. The remaining articles were evaluated for quality using the Joanna Briggs Institute Check.ist for Diagnostic Test Accuracy Studies. The resulting list of articles was used to perform a comprehensive narrative review of the diagnostic value of imaging modalities for the diagnosis of POP. Results The original search yielded 3,289 references, 135 of which were used by the writing group. Conclusions Most imaging studies utilized in the diagnoses of POP lack.d standardization in the definition of POP. Most imaging studies lack.standardization in the protocols used to diagnose POP within each imaging technique. Ultrasound- and MRI-related studies are most represented in the literature, compared with fewer CT- and X-ray-/fluoroscopy-related studies. Therefore, radiographic imaging is of limited value in the diagnosis of POP.

关键词： Imaging Ultrasound MRI CT scan Fluoroscopy Pelvic organ prolapse Diagnosis

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Circulating microRNA profiling identifies microRNAs link.d to prediabetes associated with alcohol dependence syndrome

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ALCOHOL 2025年 122卷 101-109页

作者： Ramaswamy, Palaniswamy Athira, S., V Misra, Pratibha Chauhan, V. S. Adhvaryu, Ark. gupta, Anurodh ankita, G. Sibin, M. k. Armed Forces Med Coll Multi Disciplinary Res Unit Pune 411040 India Armed Forces Med Coll Dept Biochem Pune 411040 India Armed Forces Med Coll Dept Psychiat Pune 411040 India

Back.round: MicroRNAs are abundant in serum and have emerged as important regulators of gene expression, implicating them in a wide range of diseases. The purpose of this study was to discover and validate serum miRNAs in prediabetes associated with alcohol dependence syndrome (ADS). Method: Serum samples from ADS patients with or without prediabetes and normoglycemic controls were subjected to microarray. Validation of identified candidate miRNAs was performed by RT-qPCR. Additionally, GO and k.GG pathway analyses were carried out to uncover target genes anticipated to be controlled by the candidate miRNAs. Results: Notably, 198, and 172 miRNAs were differentially expressed in ADS-patients with or without prediabetes compared to healthy controls, and 7 miRNAs in ADS-patients with prediabetes compared to ADS-normoglycemic patients, respectively. Furthermore, hsa-miR-320b and hsa-miR-3135b were differentially expressed exclusively in ADS-patients with prediabetes, and this was further validated. Interestingly, GO and k.GG pathway analysis revealed that genes predicted to be modulated by the candidates were considerably enriched in numerous diabetes-related biological processes and pathways. Conclusion: Our findings revealed that ADS-patients with or without prediabetes have different sets of miRNAs compared to normoglycemic healthy subjects. We propose serum hsa-miR-320b and hsa-miR3135b as potential biomark.rs for the diagnosis of prediabetes in ADS-patients. (c) 2024 Elsevier Inc. All rights reserved.

关键词： Alcohol dependence syndrome prediabetes microarray miRNA hsa-miR-320b hsa-miR-3135b

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Solution processable perovsk.te-hybrid heterojunction silicon 4T tandem solar cells

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MATERIALS TODAY ADVANCES 2025年 25卷

作者： Afroz, Mohammad Adil Sharma, Bhavna Sharma, Ruchi k. gupta, Ritesh k.mar Sengupta, ankita Srivastava, Sanjay k. Satapathi, Soumitra Indian Inst Technol Roorkee Dept Phys Roorkee 247667 Uttarakhand India CSIR Natl Phys Lab Adv Mat & Device Metrol Div Photovolta Metrol Sect New Delhi 110012 India Acad Sci & Innovat Res AcSIR Ghaziabad 201002 India Indian Inst Technol Roorkee Ctr Sustainable Energy Roorkee 247667 Uttarakhand India

Solar photovoltaic technology has witnessed significant advancements through the development of novel hybrid heterojunction solar cells (HHSCs). However, there is scope for enhancement of power conversion efficiency (PCE) of the HHSCs. Here, a solution-based fabrication approach involving a high-performance semi-transparent perovsk.te cell (ST-PSC) stack.d in tandem with a hybrid heterojunction silicon solar cell (HHSC) has been demonstrated for efficiency enhancement. The proposed 4-terminal tandem architecture exploits light management through the bandgap matching of the perovsk.te cell with silicon to minimize thermalization and absorption losses. This has led to a significant 41 % enhancement in the PCE of the HHSC. The experimental results are also correlated with the help of SCAPS 1D simulation. These low cost tandem solar cells hold the potential for upscaling and commercialization.

关键词： Hybrid heterojunction solar cell Solution processable Semi-transparent perovsk.te cell Tandem SCAPS 1D

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Identification of heat tolerant lentil genotypes through stress tolerance indices

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SCIENTIFIC REPORTS 2025年第1期15卷 1-17页

作者： Sark.r, Rohit Mishra, Gyan Prak.sh Premak.mar, ankita Singh, ankita Roy, Jenia Shivaprasad, k.mbarahally Murthigowda Reddappa, Shashidhar B. k.mar, Sudhir Bansal, Ruchi Dasgupta, Uttarayan gupta, Soma Sark.r, Susheel k.mar gupta, Sanjeev k.mar, Shiv Dik.hit, Harsh k. Yadav, Prachi Sripathrao Ask., Muraleedhar S. Indian Agr Res Inst Div Genet New Delhi India Indian Agr Res Inst Div Seed Sci & Technol New Delhi India Indian Council Forestry Res & Educ ICFRE Inst Forest Biodivers Hyderabad India Indian Council Agr Res ICAR Krishi Vigyan Kendra VPKAS Almora Almora India Indian Agr Res Inst Div Plant Physiol New Delhi India Indian Agr Res Inst Div Design Expt New Delhi India Indian Council Agr Res ICAR Krishi Bhavan New Delhi India Int Ctr Agr Res Dry Areas South Asia & China Program Natl Agr Sci Complex New Delhi India

With climate change projections indicating an increase in the frequency of extreme heat events and irregular rainfall patterns globally, the threat to global food security looms large. Terminal heat stress, which occurs during the critical reproductive stage, significantly limits lentil productivity. Therefore, there is an urgent need to improve lentil's resilience to heat stress to sustain production. However, studies identifying heat-tolerant sources in lentils are limited. To address these issues, we assessed 158 lentil genotypes under normal and late-sown conditions over two consecutive seasons. We employed eleven heat stress indices to identify lines tolerant to heat stress. All genotypes exhibited a decrease in average grain yield when subjected to stress conditions as compared to non-stress conditions, indicating the impact of heat stress on crop yield. Correlation analysis showed significant positive correlation between yield in normal and late-sown conditions and the following heat stress indices: STI, MP, MRP, YI, GMP, and HM. In contrast, TOL, SSPI, and PYR showed negative associations with yield in late-sown conditions. Based on these indices, we identified the genotypes P13143, P13130, and P13135 as high-yielding in both stress and non-stress conditions. Cluster analysis and biplot display in PCA also confirmed that genotypes P13143, P13130, and P13135 exhibited suitability and high yield potential in both environments. These genotypes can be utilized as donors in future breeding programs to introduce genetic variations for improving heat stress tolerance in lentil.

关键词： Abiotic stress Stress tolerance indices Lentil Climate smart

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Transforming CO₂ into formic acid by integrated solar-driven catalyst-enzyme coupled artificial photosynthetic system

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Photochemistry and Photobiology 2025年第0期 2025 Jan 31页

作者： Singh, ankita Yadav, Rajesh k. gupta, Abhishek.k.mar Singh, Chandani Sharma, k.nchan Mishra, Shaifali Shahin, Rehana Singh, Atul P. Yadav, k.ishna k.mar Baeg, Jin-Ook Department of Chemistry and Environmental Science Madan Mohan Malaviya University of Technology Gorakhpur India Department of Physics School of Basic and Applied Sciences Harcourt Butler Technical University Kanpur India Department of Chemistry Central University South Bihar Patna India Korea Research Institute of Chemical Technology Daejeon South Korea Faculty of Science and Technology Madhyanchal Professional University Bhopal India

Photo-biocatalyst coupled systems offer a promising approach for converting solar energy into valuable fuels. The bio-integrated photocatalytic system sets a research benchmark.by utilizing green energy for formic acid production, reducing CO₂ emissions, and enhancing selectivity through bio-enzyme incorporation. This bio-photocatalytic are promising solutions for environmental remediation and energy production. This research reports the synthesis and application of a novel metal-free, nitrogen-enriched graphene composite photocatalyst (N_enGCTPP) for artificial photosynthesis. N_enGCTPP was synthesized by covalently coupling tetraphenyl porphyrin tetracarboxylic acid (TPP) with N-doped graphene via a polycondensation pathway. The photogenerated charge separation then facilitates the regeneration of enzymatically active coenzymes (NADH) for formic acid production catalyzed by formate dehydrogenase. The photocatalyst exhibited remark.ble performance in photocatalytic regeneration of the coenzyme NADH from NAD⁺ with a high yield of 41.80%, as well as photocatalytic production of formic acid (HCO₂H) as a solar fuel from CO₂ with a yield of 99.12 μM. This innovative artificial photosynthetic system demonstrates an affordable, highly efficient, and selective approach for converting carbon dioxide into valuable solar fuels and regenerating NADH, addressing environmental concerns and contributing to sustainable energy solutions. © 2025 The American Society for Photobiology.

关键词： NenGCTPP photocatalyst NADH regeneration solar fuel TPP Z-scheme

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