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Magnetic dispersive solid-phase extraction for the determination of three different glycosides in the Kang'ai injection

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MICROCHEMICAL JOURNAL 2019年 146卷 575-581页

作者： li, Peng Ye, Junfeng Zhang, Yue Wang, Ziqi Ren, Sujuan Jin, Yongri li, xuwen Jilin Univ Coll Chem Changchun 130012 Jilin Peoples R China Jilin Univ Hosp 1 Changchun 130012 Jilin Peoples R China

In this work, the content of flavonoid glycosides and astragalosides in a Chinese medicine injection was determined by using the magnetic dispersive solid phase extraction method coupled with high performance liquid chromatography with an evaporative light-scattering detector (HPLC/ELSD). Multi-walled carbon nanotubes (MWCNTs) modified by Fe3O4 nanoparticles obtained in a one-step synthetic procedure, were used as a sorbent to conveniently remove the analytes from water. Single factor experiments were performed to investigate the factors that affected the extraction efficiency, such as the hydroxyl content of the modified MWCNTs, the amount of sorbent, the type of elution solvent, the volume of elution solvent, sample pH and salting-out effect. Under the appropriate optimal conditions, the limits of detection (LODs) and limits of quantification (LOQs) for the three glycosides ranged from 0.16 mu g/mL to 0.60 mu g/mL and 0.58 mu g/mL to 1.24 mu g/mL, respectively. Moreover, the intra- and interday precision ranged from 1.9% to 2.9% and 2.4% to 4.5%, respectively. Meanwhile, the recoveries of spiked samples were measured, which ranged from 91.1% to 96.2% with relative standard deviations (RSDs) ranging from 2.8% to 3.8%. Accordingly, the application of the proposed method for the analysis of Chinese medicine injection samples offered several advantages: an acceptable precision, low LODs and good recoveries. This work might provide a research basis for the quality control of Chinese medicine injections and the further study of the Kang'ai injection.

关键词： Dispersive solid phase extraction Evaporative light-scattering detector Kang'ai injection

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Centrifugation free and air-assisted liquid-liquid microextraction based on deep eutectic solvent for determination of rare ginsenosides in Kang'ai injection

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MICROCHEMICAL JOURNAL 2018年 142卷 313-320页

作者： li, Peng Ye, Junfeng Zhang, Yue Wang, Ziqi Ren, Sujuan li, xuwen Jin, Yongri Jilin Univ Coll Chem Changchun 130012 Jilin Peoples R China Jilin Univ Hosp 1 Changchun 130012 Jilin Peoples R China

A simple, effective and convenient enrichment method coupled with high performance liquid chromatography (HPLC) was established for the analysis of rare ginsenosides in Kang'ai (KA) injection. Air assisted liquid-liquid microextraction was used in this work for the first time to determine the rare ginsenosides in KA injection. Deep eutectic solvent (DES) and tetrahydrofuran (THF) were specially selected as a novel green extraction solvent and an emulsified solvent, respectively. To remove the DES droplets, Fe3O4 nanoparticles were used. Therefore, centrifugation was free in this work, which can increase the efficiency of analysis procedure. Moreover, Fe3O4 nanoparticles were reusable. To achieve better extraction efficiency and enrichment factor, the experimental conditions have been optimized, including type and volume of DES and emulsified solvent, amount of Fe3O4 nanoparticles and inorganic salt, and pH value. Under the optimum extraction condition, the validation parameters show the limits of detection (LODs) are in the range from 10.2 ng/mL to 137.8 ng/mL and the limits of quantity (LOQs) are in the range from 55.5 ng/mL to 428.5 ng/mL. The recoveries of analytes range from 91.3% to 106.7% with the relative standard deviations (RSDs) ranging from 1.2% to 4.5%. The recommended approach was utilized to acquire the information of constituents in KA injection. Moreover, the results in this research could provide a study basis for further study of KA injection.

关键词： Deep eutectic solvent liquid-liquid microextraction Rare ginsenosides Kang'ai injection HPLC

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Iron-tetrasulfophthalocyanine functionalized graphene nanosheets: Attractive hybrid nanomaterials for electrocatalysis and electroanalysis

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JOURNAL OF ELECTROANALYTICAL CHEMISTRY 2011年第1期651卷 12-18页

作者： li, Nan Zhu, Mingfang Qu, Meili Gao, Xia li, xuwen Zhang, Weide Zhang, Jiaqi Ye, Jianshan Tianjin Univ Technol Coll Environm Sci & Safety Engn Tianjin 300071 Peoples R China S China Univ Technol Coll Chem & Chem Engn Guangzhou 510641 Guangdong Peoples R China

We report the characteristics and electrochemical sensing features of iron-tetrasulfophthalocyanine (FeTSPc)-functionalized graphene nanosheets (GNs) composites. The noncovalently FeTSPc-functionalized GNs (GNs-FeTSPc) possess an improved solubility in aqueous solution and the GNs-FeTSPc film electrode exhibits an enhanced electrocatalytic activity towards oxidation of isoniazid (INZ) and uric acid (UA). Direct electrochemistry of GNs-FeTSPc nanocomposites shows that GNs could facilitate the electron transfer between glass carbon electrodes (GCEs) and the electroactive center of FeTSPc. Additionally, a comparative study using different carbon nanomaterials reveals that the functionalized GNs have better electrocatalytic ability than that of multi-walled carbon nanotubes (MWCNTs) and graphite while no obvious enhancement is obtained on mesoporous carbons (MPC) and fullerene (C-60). Hence, the GNs with unusual structure are suitable for preparing functionalized nanocomposite as a promising electrochemical sensing platform. (c) 2010 Elsevier B.V. All rights reserved.

关键词： Graphene Iron-tetrasulfophthalocyanine Electrocatalysis Isoniazid Uric acid

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A review: The structures and bioactivities of steroidal saponins from Allium macrostemon Bulbus

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PHYTOCHEMISTRY LETTERS 2023年第1期57卷 210-226页

作者： Xie, Huili Shi, Xiaolei Zhao, Dexin Wang, Bingzi Jin, Yongri li, xuwen Jilin Univ Coll Chem 2519 Jiefang Rd Changchun 130021 Peoples R China Jilin Univ Coll Food Sci & Engn Changchun 130062 Peoples R China

Allium macrostemon Bulbus (Xiebai in Chinese), as an important medicinal and edible homologous plant, has two kinds of original plants (the dry bulbs of Allium macrostemon and Allium chinense). In China, the dry bulbs of Allium macrostemon and Allium chinense have been used to treat some diseases for thousands of years. Steroidal saponins are considered to be one type of their most important active ingredients and research into the phar-macological activities of these compounds are currently undertaken. Some steroidal saponins may be promising therapeutic tools, which has caught the attention of researchers. This article provides a comprehensive overview of 102 steroidal saponins and sapogenins found in Allium macrostemon Bulbus, along with their reported phar-macological activities, including anti-inflammatory, antioxidant, antiplatelet aggregation, blood lipid-lowering, antitumor, and other beneficial effects. In this review, the extraction and isolation, spectral characteristics and pharmacological activities of steroidal saponins have been summarized based on literature from 1989 to 2022.

关键词： Allium macrostemon Bulbus Allium macrostemon Allium chinense Steroidal Saponins Chemical structure Bioactivity

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Application of Cu²⁺-based electron spin resonance spectroscopy in measurement of antioxidant capacity of fruits

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ANALYTICAL AND BIOANALYTICAL CHEMISTRY 2019年第25期411卷 6677-6686页

作者： Tian, Sizhu li, xuwen Jiang, Jia Wang, Kun Zhang, Hanqi Yu, Aimin Zhang, Ziwei Jilin Univ Coll Chem Qianjin St 2699 Changchun 130012 Jilin Peoples R China

The antioxidant capacity of 22 kinds of fruits was measured by the developed electron spin resonance (ESR) method based on Cu2+ sensor. Cu2+ is reduced to Cu+ by the antioxidants in the fruits, and the remaining Cu2+ was determined by ESR and UV-Vis spectroscopy. Cu2+ can give an ESR signal whereas Cu+ cannot, and the loss of the ESR signal was used to quantify the antioxidant capacity of various fruits. The results were shown as vitamin C equivalent antioxidant capacity (VCEAC). The VCEAC values obtained by ESR and UV-Vis methods ranged from 24.23 to 688.61 mg/100 g and from 24.12 to 677.79 mg/100 g, respectively. Cupric ion reducing antioxidant capacity (CUPRAC) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) methods were employed for comparison. Based on Pearson's correlation test, the results obtained by CUPRAC and DPPH methods were both significantly correlated with these obtained by the present method, which indicated that the novel method was reliable. Total phenolic content for all kinds of fruits was measured with the Folin-Ciocalteu reagent, and VCEAC values obtained by the ESR method were significantly correlated with total phenolic contents.

关键词： Electron spin resonance Cu2+ reduction Fruits Antioxidant capacity VCEAC Total phenolic content

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Ultrasonic-Assisted Nebulization Extraction Coupled with SPE and HPLC for the Determination of Triterpenoids in Root of Euphorbia pekinensis Rupr.

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CHROMATOGRAPHIA 2013年第15-16期76卷 967-974页

作者： Zhang, Peixu Jin, Yongri Chen, Jiafeng Yao, Hua Zhang, Hanqi Yu, Aimin li, xuwen Jilin Univ Coll Chem Changchun 130012 Peoples R China Jilin Univ Hosp 1 Dept Neurol Changchun 130012 Peoples R China

In this work, a novel method based on ultrasonic-assisted nebulization extraction coupled with solid phase extraction (UANE-SPE) and determined by high performance liquid chromatography was developed for the determination of triterpenoids in root of Euphorbia pekinensis Rupr. The experimental conditions for the UANE-SPE, such as type of extraction solvent, sample amount, type and amount of sorbent, extraction time and volume of the elution solvent, were examined and optimized. The method was successfully applied to determine euphol and tirucallol in the dried root of E. pekinensis Rupr. The recoveries of the analytes were in the range of 89.1-102.0 %. The limits of detection were 12 mu g g(-1) for tirucallol and 10 mu g g(-1) for euphol. The extraction yields obtained by the proposed method are higher than those obtained by the conventional extraction methods, such as reflux and ultrasonic-assisted extraction. Compared with the traditional methods, the proposed method can reduce the consumption of the labor, shorten the sample preparation time and increase the efficiencies in the extraction of active constituents from plant materials.

关键词： High performance liquid chromatography Ultrasonic-assisted nebulization extraction Solid phase extraction Triterpenoids Euphorbia pekinensis Rupr.

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Protective effect of total flavonoids extracted from the leaves of Murraya paniculata (L.) Jack on diabetic nephropathy in rats

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FOOD AND CHEMICAL TOXICOLOGY 2014年 64卷 231-237页

作者： Zou, Jingtao Yu, Xiaofeng Qu, Shaochun li, xuwen Jin, Yongri Sui, Dayuan Jilin Univ Dept Pharmacol Sch Pharmaceut Sci Changchun 130021 Peoples R China Jilin Univ Dept Analyt Chem Coll Chem Changchun 130021 Peoples R China

This study was designed to evaluate the effects of total flavonoids extracted from the leaves of Murraya paniculata (L) Jack (TFMP) on diabetic nephropathy. High fat diet and streptozotocin-induced diabetic rats were treated with the TFMP (35 or 70 mg/kg) for 13 weeks. Changes of renal function parameters were examined at the end of administration. Some kidneys were collected for histological and immunohistochemistry studies, the other ones for biochemical parameters analysis. TFMP significantly decreased the levels of serum blood urea nitrogen, serum creatinine, creatinine clearance, interleukin-6, urinary albumin, 24 h-urinary albumin excretion rate, kidney weight to body weight ratio and fasting blood glucose in diabetic rats. Meanwhile, the levels of triglycerides, total and LDL cholesterols in the TFMP treated diabetic rats were lower and the high-density lipoprotein cholesterol level was higher than that in the diabetic rats. TFMP treatment significantly blocked the decrease of superoxide dismutase and glutathione peroxidase and increase of malondialdehyde levels in diabetic rats. Furthermore, the TFMP not only decreased the expression of TGF-beta 1 and CTGF protein, but also reduced diabetes-induced morphological alterations of the kidney. These results suggest that TFMP is a protective agent against renal damage in diabetic nephropathy. (C) 2013 Elsevier Ltd. All rights reserved.

关键词： Murraya paniculata (L.) Jack High fat diet Streptozotocin Renal damage Diabetic nephropathy Rat

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Anti-allergic activity of 3, 5, 6, 7, 8, 3, 4-heptamethoxyflavone extracted from Citri Reticulatae Pericarpium

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SPECTROSCOPY LETTERS 2018年第5期51卷 236-243页

作者： Shi Xiaolei Wang Bo Zhang Lu Wang Ying Yang Jie li xuwen Jin Yongri Jilin Univ Coll Food Sci & Engn Changchun Jilin Peoples R China Jilin Univ Coll Chem Changchun 130012 Jilin Peoples R China

The inhibitory activity of 3, 5, 6, 7, 8, 3, 4-heptamethoxyflavone extracted from Citri Reticulatae Pericarpium on RBL-2H3 cells degranulation was done by two different methods. At low concentration, the values of inhibition obtained by modified method were much larger than that of the classical method. Spectroscopic measurements and molecular docking calculation were applied to study the mechanism. It was induced by interaction between flavonoid and immunoglobulin E. Compared with the interaction between antibody and receptor, the force was weak, the inhibitory effect on -hexosaminidase release was strong, with some structural changes to the antibody.

关键词： Anti-allergic activity IgE inhibition RBL-2H3 spectroscopic method Flavonoid 3 5 6 7 8 3 4-heptamethoxyflavone 3D fluorescence spectra three-dimensional fluorescence spectra

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Oral Intervention of Narirutin Ameliorates the Allergic Response of Ovalbumin Allergy

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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2022年第41期70卷 13313-13326页

作者： Shi, Xiaolei Zhao, lina Niu, liyan Yan, Yixuan Chen, Qiushi Jin, Yongri li, xuwen Jilin Univ Coll Food Sci & Engn Changchun 130012 Peoples R China Jilin Univ Coll Chem Changchun 130012 Peoples R China

A new intervention was investigated for the induction of oral tolerance (OT) of OVA using narirutin by in vivo and in vitro experiments combined with network pharmacology and structural analysis of molecular docking. Narirutin (and its metabolism naringenin) has effects on OT by affecting B cell function, DCs, and T cell response by prediction. It was verified that narirutin could affect B cell function of secreting antibodies, thereby reducing the ability of DCs to absorb antigens by affecting GATA3, CCR7, STAT5, and MHCII expression and regulating T cell response by suppressing Th2 and improving Treg cells in vivo. Molecular docking showed that steric hindrance effects may be the reason for weaker binding energy with targets of narirutin. However, this does not mean that it has no bioactivity, for it can inhibit mast cell degranulation. This finding is interesting because it offers the possibility of using natural compounds to promote oral tolerance.

关键词： oral tolerance narirutin food allergy OVA intervention network pharmacology

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A FRET-based fluorescent probe for mercury ions in water and living cells

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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 2016年 165卷 99-105页

作者： Zhang, Bo Ma, Pinyi Gao, Dejiang Wang, Xinghua Sun, Ying Song, Daqian li, xuwen Jilin Univ Coll Chem Qianjin St 2699 Changchun 130012 Peoples R China Changchun Jilin Univ Little Swan Instruments Co Ltd Chuangxin Rd 1203 Changchun 130012 Peoples R China

On the basis of fluorescence resonance energy transfer (FRET), a new rhodamine derivative (DRh) was synthesized as a ratiometric fluorescent probe for detecting Hg2+ in water and living cells samples. The recognition properties of the probe DRh with metal ions had been investigated in H2O/CH3CN (9:1, v/v;Tris-HCl 50 mmol L-1;pH = 7.0) solution by the UV-Vis spectrophotometry and the fluorescence spectrophotometry. The results showed that the probe DRh exhibited the selective recognition of Hg2+. Upon the addition of Hg2+, the spirolactam ring of probe DRh was opened. The 1:1 stoichiometric structure between DRh and Hg2+ were supported by Job's plot, MS and OFT theoretical calculations. The linearly fluorescence intensity ratio (I-582/I-538) is proportional to the concentration of Hg2+ in the range 0-30 mu mol L-1. The limit of detection (LOD) of Hg2+ is 0.008 mu mol L-1 (base on S/N = 3). The present probe was applied to the determination of Hg2+ in neutral water samples and gave recoveries ranging from 104.5 to 107.9%. Furthermore, the fluorescent probe also can be applied as a bioimaging reagent for Hg2+ detection in HeLa cells. (C) 2016 Elsevier B.V. All rights reserved.

关键词： Mercury ions Fluorescence probe Rhodamine FRET Water living cells DFT

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