Polyurethane Porous Elastic Road Surface (PERS) has the characteristics of energy saving, emission reduction, carbon reduction, drainage, noise reduction, and de-icing. However, the aging properties of PERS have been ...
详细信息
The Rabies virus, a single-strand RNA virus with a negative-sense polarity, is responsible for causing encephalitis and is a zoonotic disease. If not promptly treated after infection, it has a close to 100 % fatality ...
详细信息
The Rabies virus, a single-strand RNA virus with a negative-sense polarity, is responsible for causing encephalitis and is a zoonotic disease. If not promptly treated after infection, it has a close to 100 % fatality rate. Similar to other negative-sense polarity single-strand RNA viruses, the Rabies virus requires the creation of a positive-strand RNA intermediate for replication. One approach to identify this replication activity is to detect the complementary strand of the viral RNA genome in suspected infected cells or tissues. The reported Rabies virus RT-qPCR detection methods are designed to detect total viral load in samples without distinguishing between positive- and negative-strand for RNA viruses. As such, in this study, a sensitive Taqman-based strand-specific RT-qPCR assay has been developed to quantitatively detect both the positive- and negative-strand of the Rabies virus. This method demonstrates good reproducibility across a wide dynamic range and exhibits linearity of 8 logs with a lower limit of detection of 103 copies/mu L for the positive-strand and 9 logs with a lower limit of detection of 102 copies/mu L for the negative-strand. Notably, it can accurately detect a specific viral RNA strand even in the presence of high levels of the opposite strand, confirming the method's specificity. In summary, a reliable strandspecific RT-qPCR assay has been developed and validated to differentiate replicating from non-replicating Rabies virus.
Pulmonary fibrosis is a serious complication in the late stage of COVID-19 and poses a great health threat to patients. Currently, adrenal glucocorticoids (GCs) are commonly used in the treatment of pulmonary fibrosis...
详细信息
Pulmonary fibrosis is a serious complication in the late stage of COVID-19 and poses a great health threat to patients. Currently, adrenal glucocorticoids (GCs) are commonly used in the treatment of pulmonary fibrosis, but their systemic side effects limit their application. The purpose of this study was to investigate the application potential of targeted delivery system of respiratory inhalation based on medical thermal radiation images in the treatment of pulmonary fibrosis of COVID-19. Multimodal imaging was used to determine the location and extent of pulmonary fibrosis. The temperature distribution of the lungs was then monitored using medical thermal imaging technology to identify areas of inflammatory activity. Based on these data, a respiratory inhalation device was designed, which can adjust the concentration and distribution of drug delivery based on the feedback of the thermal radiation image, ensuring that the drug can accurately act on the inflammation area. Through clinical trials, we have found that this targeted delivery system can significantly increase the concentration of the drug in the diseased area while reducing systemic side effects. Medical thermal radiation images showed that the temperature of the patient's lung inflammation area dropped significantly after treatment, indicating that the inflammation was effectively controlled. CT and MRI images also showed a reduction in the degree of pulmonary fibrosis. Therefore, the respiratory inhalation targeted delivery system based on medical thermal radiation images has shown significant clinical application prospects in the treatment of COVID-19 pulmonary fibrosis. This technology can not only improve the accuracy of treatment, reduce the side effects of drugs, but also monitor the effect of treatment in real time, providing a new idea for personalized medicine.
The present study aims to explore potential therapeutic effects of Naringin on osteoarthritis (OA) and investigate the underlying mechanism. The chondrocytes in the joint usually undergo detrimental changes during OA ...
详细信息
The present study aims to explore potential therapeutic effects of Naringin on osteoarthritis (OA) and investigate the underlying mechanism. The chondrocytes in the joint usually undergo detrimental changes during OA progression, including increased apoptosis. miRNAs emerge as crucial regulators in this processes. The study delves into the intricate interplay between miR-29a-3p, BAX-mediated apoptosis, and Naringin intervention. Pro-inflammatory cytokines induce chondrocyte apoptosis in OA, impacting cell viability. Naringin treatment effectively restores cell survivability (1.8-fold change), inhibiting caspase activity (0.54-fold change) and lowering matrix metalloproteinases-9 (MMP-9) (0.50-fold change) and MMP-13 expression (0.50-fold change). Furthermore, COL2A1, Sox9, Runx2, TGF-β1, and BMP-4 levels in cytokines-stimulated chondrocytes were enhanced by Naringin, accompanied by decreased productions of MMP3 and MMP13. In cartilage tissues of OA rats, Osteoarthritis Research Society International (OARSI) scores in Safranin O staining were elevated, Pro-inflammatory cytokine productions and MMP3 and MMP13 expressions were enhanced, and COL2A1, Sox9, Runx2, TGF-β1, and BMP-4 levels were reduced, which were remarkably rescued by Naringin. We further revealed the intricate connection between miR-29a-3p and the chondrocyte fate. Elevated miR-29a-3p expression corresponds to increased apoptotic chondrocytes. Naringin suppresses miR-29a-3p, curbing apoptosis and suggesting a potential therapeutic avenue. Notably, BAX emerges as a key player, with miR-29a-3p influencing its expression. Naringin's mitigation of BAX upregulation underscores its protective role. Overall, we found the potential role of Naringin in addressing chondrocyte apoptosis in OA through miR-29a-3p-BAX modulation, offering insights into innovative OA management strategies.
暂无评论