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Rapid masculinization and effects on the liver of female western mosquitofish (Gambusia affinis) by norethindrone

CHEMOSPHERE 2019年 216卷 94-102页

作者： Hou, Liping Chen, Shangduo Chen, Hongxing Ying, Guangguo Chen, Diyun Liu, Juan liang, Ye Wu, Rongrong Fang, xuwen Zhang, Cuiping Xie, Lingtian Guangzhou Univ Sch Life Sci Guangzhou 510655 Guangdong Peoples R China South China Normal Univ Environm Res Inst MOE Key Lab Theoret Chem Environm Guangzhou 510006 Guangdong Peoples R China Guangzhou Univ Sch Environm Sci & Engn Guangdong Prov Key Lab Radionuclides Pollut Contr Guangzhou 510006 Guangdong Peoples R China Guangzhou Univ Minist Educ Inst Environm Res Greater Bay Innovat Ctr Guangzhou 510006 Guangdong Peoples R China Guangzhou Univ Minist Educ Key Lab Waters Safety & Protect Pearl River Delta Guangzhou 510006 Guangdong Peoples R China

Natural and synthetic progestins in receiving streams can disrupt the normal endocrine systems of fish. Norethindrone (NET) is a widely used synthetic progestin that often appears in wastewater effluents. For this research, adult female western mosquitofish (Gambusia affinis) were exposed to NET at three concentrations. The effects of NET on the following biological factors were evaluated: the histology of the ovaries and livers, the anal fin morphology, and transcription of genes related to steroidogenesis signaling pathways in the livers. After 42 d exposure to NET at 33.0 ng L-1 and 347.5 ng L-1, rapid masculinization, an increase in the number of atretic and postovulatory follicles in the ovary, enhanced vascularization, degenerated hepatocytes and irregular nuclei in the livers were observed. Exposure to NET did not affect the expression of the androgenic and estrogenic receptor genes and Cyp19a except for a significant up-regulation of Er alpha. However, the expression of Vtg A, Vtg B, and Vtg C were markedly inhibited in the females exposed to three concentrations of NET. Compared to the control female, exposure to NET at 33.0 ng L-1 and 347.5 ng L-1 caused a 4.4- and 5.8-fold increase in the expression of Hsd17 beta 3 in the livers, respectively. The results demonstrate that NET can cause rapid masculinization of female G. affinis, hepatopathological alterations and inhibited expressions of Vtg A, Vtg B, and Vtg C. The results imply that G. affinis populations might be threatened in NET-contaminated environment. (C) 2018 Elsevier Ltd. All rights reserved.

关键词： Norethindrone Gambusia affinis Masculinization Progestins Endocrine disrupting chemicals

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脑膜炎球菌疫苗多糖及多糖蛋白结合物分子量尺寸排阻色谱-多角度激光光散射联用检测方法的建立及验证

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中国生物制品学杂志 2024年第10期37卷 1230-1238页

作者：邹剑杨蕾李茂光叶强焦旭雯梁蔚阳郭中平马晶姚宇峰李炎四川省药品检验研究院国家药品监督管理局疫苗及生物制品质量控制与评价重点实验室四川成都6100972 中国食品药品检定研究院北京102629 广东省药品检验所广东广州510663 国家药典委员会北京100061 中国医学科学院生物学研究所云南昆明650118

目的建立尺寸排阻色谱-多角度激光光散射(size exclusion chromatography-multiangle laser-light scattering,SEC-MALLS)联用测定脑膜炎球菌多糖及多糖蛋白结合物分子量的方法,并进行验证及初步应用,以期进一步提高该品种质量控制水平... 详细信息

目的建立尺寸排阻色谱-多角度激光光散射(size exclusion chromatography-multiangle laser-light scattering,SEC-MALLS)联用测定脑膜炎球菌多糖及多糖蛋白结合物分子量的方法,并进行验证及初步应用,以期进一步提高该品种质量控制水平。方法建立SEC-MALLS联用方法检测脑膜炎球菌多糖及多糖蛋白结合物分子量,色谱条件:流动相为0.9%氯化钠溶液,色谱分离柱为Shodex OHpak LB-806 HQ(300 mm×8.0 mm,13μm),流速为0.5 mL/min,柱温为35℃。对方法的系统适用性、准确性、精密性、耐用性进行验证,并用该方法检测不同厂家来源的样品,同时与现行药典质量标准方法进行相关性分析。结果普鲁兰糖标准品除STD P-100以外,STD P-50 STD P-800重均分子量(Mw)实测值与标示值相对误差和精密度小于5.0%,可作为系统适应性样品;重复性、中间精密度RSD均不大于5.0%。多糖及多糖蛋白结合物在配制完成48 h内稳定性良好。A、C、Y、W135群多糖平均Mw分别为257.5、377.3、399.7、305.5 kDa;A、C群多糖蛋白结合物分别为6438、23360 kDa。71批多糖样品中有5批次A、C群结果超趋势,其余批次批内和批间差异均在均值±3 SD的范围以内。SEC-MALLS法与现行药典质量标准方法测定结果除Y群多糖分配系数(KD)与Mw呈一定的负相关性外,两种方法在其余多糖及多糖蛋白结合物中相关性并不显著。结论SEC-MALLS方法能直接测定脑膜炎球菌疫苗多糖及多糖蛋白结合物分子量,具有操作简单、快速、准确、能一定程度反映分子结构信息等优点,有利于提升该关键质量参数的质量控制水平。

关键词：多角度激光光散射尺寸排阻色谱凝胶渗透色谱脑膜炎球菌多糖多糖蛋白结合物重均分子量分子构象和粒径

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重组人酸性成纤维细胞生长因子联合夫西地酸乳膏局部应用对深Ⅱ度烧伤的影响

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中国美容整形外科杂志 2022年第7期33卷 413-416,434页

作者：李亮王旭文何丽彩李双蔺利剑赵龙刚李雯河北中石油中心医院整形手外烧伤科河北廊坊065000 廊坊永清康定精神病医院河北廊坊065600

目的观察重组人酸性成纤维细胞生长因子联合夫西地酸乳膏局部应用对深Ⅱ度烧伤患者的影响。方法选取2019年6月至2020年5月河北中石油中心医院整形手外烧伤科治疗的120例深Ⅱ度烧伤患者为研究对象,采用随机数字表法对患者分组:对照组1... 详细信息

目的观察重组人酸性成纤维细胞生长因子联合夫西地酸乳膏局部应用对深Ⅱ度烧伤患者的影响。方法选取2019年6月至2020年5月河北中石油中心医院整形手外烧伤科治疗的120例深Ⅱ度烧伤患者为研究对象,采用随机数字表法对患者分组:对照组1、对照组2、观察组,每组40例患者。对照组1采用削痂术联合重组人酸性成纤维细胞生长因子治疗,对照组2采用削痂术联合夫西地酸乳膏治疗,观察组采用削痂术联合重组人酸性成纤维细胞生长因子和夫西地酸乳膏治疗,3组患者均连续治疗至创面愈合。治疗前、治疗第20天测量白细胞介素-2(interleukin-2, IL-2)、肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)、白细胞介素-10(Interleukin-10, IL-10)、白细胞介素-6(interleukin-6, IL-6)、血管内皮生长因子(vascular endothelial growth factor, VEGF)、表皮细胞生长因子(epidermal cell growth factor, EGF)、金属蛋白酶组织抑制因子1(tissue inhibitor of metalloproteinase 1, TIMP-1)、转化生长因子β1(transforming growth factor β1, TGF-β1)水平,观察患者创面愈合时间,采用温哥华瘢痕量表(vancouver scar scale, VSS)评价3组患者瘢痕增生程度,记录患者不良反应情况。结果观察组TNF-α、IL-6水平低于对照组1和对照组2(P<0.05),观察组IL-2、IL-10水平高于对照组1和对照组2(P<0.05),观察组VEGF、EGF、TIMP-1、TGF-β1水平高于对照组1和对照组2(P<0.05),治疗第10、15、20天,3组创面愈合率升高(P<0.05),观察组创面愈合率高于对照组1和对照组2(P<0.05),观察组创面愈合平均时间低于对照组1和对照组2(P<0.05),观察组瘢痕血管分布、颜色、厚度和柔软度评分低于对照组1和对照组2(P<0.05),3组均无明显不良反应发生。结论重组人酸性成纤维细胞生长因子联合夫西地酸乳膏局部应用治疗深Ⅱ度烧伤患者,可降低促炎因子TNF-α、IL-6水平,提升抑炎因子IL-2、IL-10水平,抑制炎症,提升VEGF、EGF、TIMP-1、TGF-β1水平,提升创面愈合率,降低瘢痕增生,安全性较高。

关键词：重组人酸性成纤维细胞生长因子夫西地酸乳膏深Ⅱ度烧伤创面愈合瘢痕增生

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Medium-temperature furnace baking of 1.3 GHz 9-cell superconducting cavities at IHEP

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SUPERCONDUCTOR SCIENCE & TECHNOLOGY 2021年第9期34卷 095005-095005页

作者： He, Feisi Pan, Weimin Sha, Peng Zhai, Jiyuan Mi, Zhenghui Dai, xuwen Jin, Song Zhang, Zhanjun Dong, Chao Liu, Baiqi Zhao, Hui Ge, Rui Zhao, Jianbing Mu, Zhihui Du, Lei Sun, liangrui Zhang, liang Yang, Conglai Zheng, Xiaobing Chinese Acad Sci Inst High Energy Phys Beijing 100049 Peoples R China Chinese Acad Sci Inst High Energy Phys Key Lab Particle Accelerat Phys & Technol Beijing 100049 Peoples R China Chinese Acad Sci Ctr Superconducting RF & Cryogen Inst High Energy Phys Beijing 100049 Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China

Recently, heat treatment in the range of 250 degrees C-500 degrees C has been attempted with the aim of improving the quality factor (Q) of superconducting radio-frequency (SRF) cavities worldwide. Herein, medium-temperature (mid-T) furnace baking experiments were conducted at the Institute of High Energy Physics to obtain higher Q values. First, 1.3 GHz 1-cell cavities received mid-T furnace baking at different temperatures, resulting in not only the improvement of Q but also an anti-Q-slope behavior. Furthermore, mid-T furnace baking was applied to six 1.3 GHz 9-cell cavities, all of which indicated higher Q and anti-Q-slope behavior. The average Q was 3.8 x 10(10) at 16 MV m(-1). The maximum gradients of the 9-cell cavities were 22.7-26.5 MV m(-1). Finally, the mid-T furnace baked 1.3 GHz 9-cell cavities were welded with helium vessel, with no degradation in the vertical tests. In addition, the mid-T furnace baking process was simplified in comparison with European X-ray Free Electron Laser and Linear Coherent Light Source because of the cancellation of light electro-polishing. This simplification is beneficial in the mass production of SRF cavities with high Q, which are widely adopted in free electron lasers and high-energy colliders.

关键词： superconducting cavity quality factor gradient medium-temperature furnace baking vertical test

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Transcriptomic and physiological changes in western mosquitofish (Gambusia affinis) after exposure to norgestrel

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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2019年 171卷 579-586页

作者： Hou, Liping Chen, Shanduo Liu, Juan Guo, Jingwen Chen, Zhong Zhu, Qiaoling Zhang, Wei Xu, Guoliang liang, Ye Wu, Rongrong Fang, xuwen Zhang, Cuiping Xing, Ke Guangzhou Univ Sch Life Sci Guangzhou 510655 Guangdong Peoples R China Shantou Univ Guangdong Prov Key Lab Marine Biotechnol Shantou 515063 Peoples R China Guangzhou Univ Key Lab Water Qual & Conservat Pearl River Delta Guangdong Prov Key Lab Radionuclides Pollut Contr Minist EducInst Environm Res Greater Bay Guangzhou 510006 Guangdong Peoples R China Guangzhou Univ Coll Environm Sci & Engn Guangzhou 510655 Guangdong Peoples R China NanWu Middle Sch Guangzhou 510655 Guangdong Peoples R China Guangzhou Tieyi Middle Sch Guangzhou 510655 Guangdong Peoples R China Rural Nonpoint Source Pollut Comprehens Managemen Guangzhou 510655 Guangdong Peoples R China

Norgestrel (NGT) is a synthetic progestin used in human and veterinary medicine. Adult female mosquitofish were exposed to NGT for 42 d at 377 ng L-1. The fin morphology and the liver transcriptome were assessed. NGT exposure increased ray 4:6 length ratio. As compared to the control, NGT treatment affected the expression of 11,772 annotated transcripts in female mosquitofish. Specifically, we found 5780 were repressed while 5992 were significantly induced. Gene ontology (GO) analysis showed that 53 KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways and 158 GO terms were significantly over expressed. Genes showing the largest magnitude of expression changes were related to fin development, androgen biosynthesis, and lipid and fatty acid metabolisms, suggesting the involvement of these biological processes in response to NGT exposure in G. affinis. This first comprehensive study on the transcriptomic alterations by NGT in G. affinis not only provides valuable information on the development of molecular markers but also opens new avenues for studies on the molecular mechanisms of effects of NGT in particular and possibly other progestins in G. affinis.

关键词： Endocrine disruption Norgestrel Secondary sexual characteristics transcriptomics Gambusia affinis

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DFT Study of Oxygenated Organic Pollutants Catalyzed by Molybdenum Oxides: Comparison of Reaction Mechanisms of MoO_x + HCHO(x = 1, 2, 3)

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Chinese Journal of Structural Chemistry 2019年第8期38卷 1229-1240页

作者： GAO Xiao-Zhen liang Yan YANG Jing MENG Xiang-Jun YANG Xiao-Chun JIA Jing-Xian xuwen-Guo Yangquan Municipal Key Laboratory of Quantum Manipulation Shanxi Institute of TechnologyYangquan045000China Department of Chemistry Tangshan Normal CollegeTangshan063000China Institute for Physical Chemistry Beijing Institute of TechnologyBeijing100081China

The reaction mechanisms between formaldehyde and MoOx(x = 1, 2, 3) have been studied thoroughly in this paper. Five reaction pathways were found in three reactions(reactions Ⅰ to Ⅲ) through studying the mechanisms of MoOx(x = 1, 2, 3) catalyzing formaldehyde. Different products were obtained from three reactions. Of all three reactions, the barrier energy of Route ⅡA is the lowest(4.70 kcal/mol), which means in MoOx(x = 1, 2, 3), MoO2 has the best catalytic effect. Compared with other similar non-toxic treatments of formaldehyde, our barrier energy is the lowest. In this research, there was no good leaving group of the compound, so the mechanisms are addition reaction. We speculate that there must be an addition reaction to the more complex reactions to molybdenum oxides and aldehydes. As a chemical reagent for removing formaldehyde, it only absorbs formaldehyde and does not emit other toxic substances outward. Molybdenum oxides retain its original structures of the final products, which means it has excellent stability in the reaction of MoOx(x = 1, 2, 3) + HCHO. The mechanisms of all three reactions are addition reactions, but they are entirely different. As the number of oxygen atoms increases, the reaction mechanisms become simpler.

关键词： reaction mechanism formaldehyde elimination molybdenum oxides addition reaction barrier energy

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自体髂骨块植骨结合Calcar螺钉重建内侧柱治疗复杂肱骨近端骨折

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中华肩肘外科电子杂志 2021年第3期9卷 244-248页

作者：祝相如张聿达李绪文刘国明梁承志扈延龄青岛大学医学部 266073 青岛大学附属医院创伤外科 266000

目的探讨自体髂骨块移植结合Calcar螺钉重建内侧柱治疗肱骨近端骨折的临床疗效。方法回顾性分析我院自2016年1月至2018年12月收治的36例单侧复杂肱骨近端骨折患者,采用自体髂骨块移植结合Calcar螺钉治疗。随访时间12~24个月,平均17.04... 详细信息

目的探讨自体髂骨块移植结合Calcar螺钉重建内侧柱治疗肱骨近端骨折的临床疗效。方法回顾性分析我院自2016年1月至2018年12月收治的36例单侧复杂肱骨近端骨折患者,采用自体髂骨块移植结合Calcar螺钉治疗。随访时间12~24个月,平均17.04个月。记录术后3、6、12个月随访时Neer肩关节功能评分(Neer评分)和肩关节功能评分(Constant评分);采用视觉模拟评分(visual analogue scale,VAS)评估患者术后疼痛程度。分析患者骨折愈合时间、并发症发生情况及肩关节活动范围。结果36例患者均达到解剖复位,骨折平均愈合时间为3.71个月,没有患者出现肱骨头缺血性坏死及内翻塌陷。术后12个月随访时,患者Neer评分(84.64±3.40)分;Constant评分(74.26±4.70)分;VAS(1.24±0.72)分。患肩关节活动度:屈(132.58±8.01)°、伸(43.75±3.41)°、内旋(58.25±4.89)°、体侧外旋(58.89±4.83)°、外展(136.46±11.04)°。结论对于内侧粉碎性复杂肱骨近端骨折,自体髂骨块植骨结合Calcar螺钉能够重建可靠的内侧支撑,降低内翻塌陷及肱骨头缺血性坏死等并发症的风险,术后功能恢复满意。

关键词：肱骨近端骨折髂骨植骨内侧柱骨移植

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High Q and high gradient performance of the first medium-temperature baking 1.3 GHz cryomodule

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Physical Review Accelerators and Beams 2024年第9期27卷 092003页

作者： Weimin Pan Jiyuan Zhai Feisi He Rui Ge Zhenghui Mi Peng Sha Song Jin Ruixiong Han Qunyao Wang Haiying Lin Guangwei Wang xuwen Dai Zhanjun Zhang Mei Li Minjing Sang liangrui Sun Rui Ye Tongxian Zhao Shaopeng Li Keyu Zhu Baiqi Liu Xiaolong Wang Xiangchen Yang Xiaojuan Bian Xiangzhen Zhang Huizhou Ma Jianbing Zhao liang Zhang Hui Zhao Runbing Guo Zhihui Mu Conglai Yang Xiaobing Zheng Chao Dong Hongjuan Zheng Zhengze Chang Xiaochen Yang Tongming Huang Qiang Ma Zihan Wang Ming Liu Wenzhong Zhou Senyu Chen Chinese Academy of Sciences Beijing 100049 China Beijing 100049 China

The world’s first 1.3 GHz cryomodule containing eight 9-cell superconducting radio-frequency (rf) cavities treated by medium-temperature furnace baking (mid-T bake) was developed at the Institute of High Energy Physics, Chinese Academy of Sciences. The 9-cell cavities in the cryomodule achieved an unprecedented high average intrinsic quality factor (Q0) of 3.8×1010 at 16 MV/m and 3.6×1010 at 21 MV/m in the horizontal test. The cryomodule can operate stably up to a total continuous wave rf voltage greater than 193 MV, with an average cavity usable accelerating gradient of more than 23 MV/m. The results significantly exceed the specifications of Circular Electron Positron Collider and Dalian advanced light source and the other high repetition rate free electron laser facilities [Linac Coherent Light Source II (LCLS-II), LCLS-II-high energy, Shanghai High Repetition Rate X-ray FEL and Extreme Light Facility, Shenzhen Superconducting Soft X-Ray Free Electron Laser, etc.]. There is evidence that the mid-T bake cavity may not require fast cooldown or long processing time in the cryomodule. This paper reviews the cryomodule performance and discusses some important issues in cryomodule assembly and testing.

关键词： Radio frequency techniques Superconducting RF

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Parasitic modes suppression in CW cold tests of 1.3 GHz 9-cell high Q cavities at IHEP

arXiv

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arXiv 2021年

作者： Mi, Zhenghui He, Feisi Pan, Weimin Sha, Peng Zhai, Jiyuan Dai, xuwen Jin, Song Zhang, Zhanjun Dong, Chao Liu, Baiqi Zhao, Hui Ge, Rui Zhao, Jianbing Mu, Zhihui Du, Lei Sun, liangrui Zhang, liang Yang, Conglai Zheng, Xiaobing Lin, Haiying Wang, Guangwei He, Xiangcong Institute of High Energy Physics Chinese Academy of Sciences Beijing100049 China Key Laboratory of Particle Acceleration Physics & Technology Institute of High Energy Physics Chinese Academy of Sciences Beijing100049 China Center for Superconducting RF and Cryogenics Institute of High Energy Physics Chinese Academy of Sciences Beijing100049 China University of Chinese Academy of Sciences Beijing100049 China

The CW RF test of 1.3 GHz 9-cell cavity in liquid helium bath at 2 K is a very important key point in the cavity procurement. Some problems can be found through the test, according which to optimized and improve the process of cavity. Recently, Medium temperature (mid-T) furnace bake of 1.3 GHz 9-cell cavities have been carried out at IHEP. Through the proceed of mid-T bake, the quality factor of cavity has been greatly improved. While the excitation of the parasitic modes in the high Q cavities CW cold test has been encountered, which implies an error source for the cavity gradient and quality factor determination. In order to ensure the testing accuracy of superconducting cavity, we have improved the testing system. Finally, the parasitic mode is completely suppressed and the CW RF cold test of high Q cavity is guaranteed. © 2021, CC BY.

关键词： Superfluid helium

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会计专业中美合作教学探讨——基于宁波工程学院会计专业教育实践

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宁波工程学院学报 2016年第1期28卷 119-123页

作者：梁旭雯姚丽琼宁波工程学院浙江宁波315016

为适应具有国际视野的高素质会计人才培养的需求,国内高校的会计专业都在尝试着多元化的国际合作项目。如引进国外的会计组织机构,与国外高校合作进行学生互访等。宁波工程学院会计专业在人才模式的改革中强调国际化会计人才培养的特色... 详细信息

为适应具有国际视野的高素质会计人才培养的需求,国内高校的会计专业都在尝试着多元化的国际合作项目。如引进国外的会计组织机构,与国外高校合作进行学生互访等。宁波工程学院会计专业在人才模式的改革中强调国际化会计人才培养的特色,其中与美国特拉华州立大学会计专业合作的"4+0"项目,则是直接引进美国会计本科教育的全部内容,与国内会计教学有机融合,促进了高素质国际化会计人才的培养。

关键词：中美合作会计专业教学改革

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