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Evolution and emergence of primate-specific interferon regulatory factor 9

JOURNAL OF MEDICAL VIROLOGY 2023年第2期95卷 e28521-e28521页

作者： Drury, Sam Claussen, Grace Zetterman, Allison Moriyama, Hideaki Moriyama, Etsuko N. N. zhang, luwen Univ Nebraska Sch Biol Sci Lincoln NE USA Univ Nebraska Ctr Plant Sci Innovat Lincoln NE USA Univ Nebraska Nebraska Ctr Virol Lincoln NE USA Univ Nebraska Ctr Plant Sci Innovat Sch Biol Sci 403 Manter Hall Lincoln NE 68588 USA Univ Nebraska Nebraska Ctr Virol Sch Biol Sci 238 Morrison Ctr Lincoln NE 68583 USA

The binding of interferon (IFN) to its receptors leads to formation of IFN-stimulated gene factor 3 (ISGF3) complex that activates the transcription of cellular IFN-regulated genes. IFN regulatory factor 9 (IRF9, also called ISGF3 & gamma;or p48) is a key component of ISGF3. However, there is limited knowledge regarding the molecular evolution of IRF9 among vertebrates. In this study, we have identified the existence of the IRF9 gene in cartilaginous fish (sharks). Among primates, several isoforms unique to old world moneys and great apes are identified. These IRF9 isoforms are named as primate-specific IRF9 (PS-IRF9) to distinguish from canonical IRF9. PS-IRF9 originates from a unique exon usage and differential splicing in the IRF9 gene. Although the N-terminus are identical for all IRF9s, the C-terminal regions of the PS-IRF9 are completely different from canonical IRF9. In humans, two PS-IRF9s are identified and their RNA transcripts were detected in human primary peripheral blood mononuclear cells. In addition, human PS-IRF9 proteins were detected in human cell lines. Sharing the N-terminal exons with the canonical IRF9 proteins, PS-IRF9 is predicted to bind to the same DNA sequences as the canonical IRF9 proteins. As the C-terminal regions of IRFs are the determinants of IRF functions, PS-IRF9 may offer unique biological functions and represent a novel signaling molecule involved in the regulation of the IFN pathway in a primate-specific manner.

关键词： evolution innate immunity interferon IRF primate

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Construction and evaluation of networks among multiple postoperative complications

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COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE 2023年 232卷 107439-107439页

作者： Shen, Yubing zhang, luwen Wu, Peng Huang, Yuguang Xin, Shijie zhang, Qiang Zhao, Shengxiu Sun, Hong Lei, Guanghua zhang, Taiping Han, Wei Wang, Zixing Jiang, Jingmei Yu, Xiaochu Chinese Acad Med Sci Inst Basic Med Sci Peking Union Med Coll Sch Basic MedDept Epidemiol & Biostat 5 Dongdansantiao St Beijing 100005 Peoples R China Chinese Acad Med Sci Peking Union Med Coll Hosp Dept Anaesthesiol Beijing Peoples R China China Med Univ Hosp 1 Dept Vasc & Thyroid Surg Shenyang Liaoning Peoples R China Qinghai Prov Peoples Hosp Dept Neurosurg Xining Qinghai Peoples R China Qinghai Prov Peoples Hosp Dept Nursing Xining Qinghai Peoples R China Cent South Univ Dept Otolaryngol Head & Neck Surg Xiangya Hosp Changsha Hunan Peoples R China Cent South Univ Dept Orthopaed Xiangya Hosp Changsha Hunan Peoples R China Chinese Acad Med Sci Peking Union Med Coll Hosp Dept Gen Surg Beijing Peoples R China Chinese Acad Med Sci Peking Union Med Coll Hosp Dept Nephrol 1 ShuaiFuYuan Beijing 100730 Peoples R China

Background and objective: Postoperative complications confer an increased risk of reoperation, prolonged length of hospital stay, and increased mortality. Many studies have attempted to identify the complex as-sociations among complications to preemptively interrupt their progression, but few studies have looked at complications as a whole to reveal and quantify their possible trajectories of progression. The main ob-jective of this study was to construct and quantify the association network among multiple postoperative complications from a comprehensive perspective to elucidate the possible evolution ***: In this study, a Bayesian network model was proposed to analyze the associations among 15 complications. Prior evidence and score-based hill-climbing algorithms were used to build the structure. The severity of complications was graded according to their connection to death, with the association between them quantified using conditional probabilities. The data of surgical inpatients used in this study were collected from four regionally representative academic/teaching hospitals in a prospective cohort study in ***: In the network obtained, 15 nodes represented complications or death, and 35 arcs with arrows represented the directly dependent relationship between them. With three grades classified on that ba-sis, the correlation coefficients of complications within grades increased with increased grade, ranging from -0.11 to -0.06, 0.16, and 0.21 to 0.4 in grade 1 to grade 3, respectively. Moreover, the probabil-ity of each complication in the network increased with the occurrence of any other complication, even mild complications. Most seriously, once cardiac arrest requiring cardiopulmonary resuscitation occurs, the probability of death will be up to 88.1%.Conclusions: The present evolving network can facilitate the identification of strong associations among specific complications and provides a basis for the development of targeted m

关键词： Postoperative complications Bayes? theorem Complication grading Network analysis Probability inference

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Maternal immune activation alters adult behavior, intestinal integrity, gut microbiota and the gut inflammation

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BRAIN AND BEHAVIOR 2021年第5期11卷 e02133页

作者： Li, Wenqiang Chen, Mengxue Feng, Xia Song, Meng Shao, Minglong Yang, Yongfeng zhang, luwen Liu, Qing Lv, Luxian Su, Xi Xinxiang Med Univ Henan Mental Hosp Affiliated Hosp 2 Xinxiang Henan Peoples R China Xinxiang Med Univ Henan Key Lab Biol Psychiat Xinxiang Henan Peoples R China Int Joint Res Lab Psychiat & Neurosci Henan Xinxiang Henan Peoples R China Henan Prov Peoples Hosp Zhengzhou Peoples R China

Background Schizophrenia is characterized by several core behavioral features, in which the gastrointestinal symptoms are frequently reported. Maternal immune activation (MIA) has been developed in a rodent model to study neurodevelopmental disorders such as schizophrenia. However, the changes in the gut environment of MIA rats remain largely unknown. Methods 10 mg/kg of polyinosinic:polycytidylic acid (Poly I:C) on gestational day 9 was intravenously administered to rats to induce MIA in order to assess changes in behavior, the intestinal barrier and microbiota in offspring. Results Maternal immune activation offspring shown increased anxiety as indicated by reduced exploration of central area in open field test and decreased exploration of open arms in elevated plus test. Cognitive impairment of MIA offspring was confirmed by reduced exploration of novel arm in Y maze test and deficiency of PPI. Intestinal muscle thickness became thinner and some specific microbial anomalies previously identified clinically were observed in MIA offspring. In addition, an increase of inflammatory responses was found in the gut of MIA offspring. Conclusions Maternal immune activation alters behavior, intestinal integrity, gut microbiota and the gut inflammation in adult offspring.

关键词： behavior gut inflammation maternal immune activation microbiota

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数字技术创新、本土市场规模与生产链延伸

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云南财经大学学报 2025年第2期41卷 33-48页

作者：周宏伟刘曙光庞素勤张露文南开大学经济学院天津300071 中国海洋大学经济学院山东青岛266100 华中科技大学经济学院武汉430074

数字技术创新是重塑国际分工优势、促进生产网络深化的重要路径。以1995—2018年45个国家的17个制造业部门为样本,实证研究数字技术创新对生产链延伸的影响,并进一步考察数字技术创新的溢出效应与本土市场规模的调节效应。研究发现,数... 详细信息

数字技术创新是重塑国际分工优势、促进生产网络深化的重要路径。以1995—2018年45个国家的17个制造业部门为样本,实证研究数字技术创新对生产链延伸的影响,并进一步考察数字技术创新的溢出效应与本土市场规模的调节效应。研究发现,数字技术创新显著促进了生产链延伸;相对于发展中国家,数字技术创新对发达国家生产链延伸的促进作用更为显著,可能存在“数字鸿沟”与“低端锁定”;数字技术创新主要促进了劳动密集型和资本密集型行业的生产链延伸,对技术密集型行业无明显影响;数字技术创新对全球价值链生产长度的促进作用高于纯国内生产和传统贸易活动。机制检验显示,数字技术创新主要通过扩大中间品进口与释放国内消费潜力两种渠道促进生产链延伸。调节效应显示,本土市场规模扩张能够强化数字技术创新对生产链延伸的促进作用,且该效应在发达国家、资本密集型行业以及全球价值链活动中更为明显。拓展性分析表明,数字技术创新具有显著的溢出效应,能够促进未发生数字技术创新行业的生产链延伸;数字技术创新质量提升同样促进了生产链延伸。研究结论对新发展格局构建和经济高质量发展具有重要启示。

关键词：数字技术创新生产链长度本土市场规模中间品进口国内消费潜力

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Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency

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NUCLEIC ACIDS RESEARCH 2021年第8期49卷 4689-4704页

作者： zhang, Qinghai He, Xiao Yao, Shihao Lin, Tianxiang zhang, luwen Chen, Danni Chen, Chao Yang, Qingxian Li, Feng Zhu, Yi-Min Guan, Min-Xin Zhejiang Univ Childrens Hosp Div Med Genet & Genom Sch Med Hangzhou 310058 Zhejiang Peoples R China Natl Clin Res Ctr Child Hlth Hangzhou 310058 Zhejiang Peoples R China Zhejiang Univ Inst Genet Sch Med Hangzhou 310058 Zhejiang Peoples R China Zhejiang Prov Key Lab Genet & Dev Disorder Hangzhou 310058 Zhejiang Peoples R China Zhejiang Univ Womens Hosp Key Lab Reprod Genet Minist Educ PRCSch Med Hangzhou 310006 Zhejiang Peoples R China Joint Inst Genet & Genome Med Zhejiang Univ & Uni Hangzhou 310058 Zhejiang Peoples R China

Deficient maturations of mitochondrial transcripts are linked to clinical abnormalities but their pathophysiology remains elusive. Previous investigations showed that pathogenic variants in MTO1 for the biosynthesis of tau m(5)U of tRNA(Glu), tRNA(Gln), tRNA(Lys), tRNA(Trp) and tRNA(Leu(UUR)) were associated with hypertrophic cardiomyopathy (HCM). Using mto1 knock-out(KO) zebrafish generated by CRISPR/Cas9 system, we demonstrated the pleiotropic effects of Mto1 deficiency on mitochondrial RNA maturations. The perturbed structure and stability of tRNAs caused by mto1 deletion were evidenced by conformation changes and sensitivity to S1-mediated digestion of tRNA(Gln), tRNA(Lys), tRNA(Trp) and tRNA(Leu(UUR)). Notably, mto1(KO) zebrafish exhibited the global decreases in the aminoacylation of mitochondrial tRNAs with the taurine modification. Strikingly, ablated mto1 mediated the expression of MTPAP and caused the altered polyadenylation of cox1, cox3, and nd1 mRNAs. Immunoprecipitation assay indicated the interaction of MTO1 with MTPAP related to mRNA polyadenylation. These alterations impaired mitochondrial translation and reduced activities of oxidative phosphorylation complexes. These mitochondria dysfunctions caused heart development defects and hypertrophy of cardiomyocytes and myocardial fiber disarray in ventricles. These cardiac defects in the mto1(KO) zebrafish recapitulated the clinical phenotypes in HCM patients carrying the MTO1 mutation(s). Our findings highlighted the critical role of MTO1 in mitochondrial transcript maturation and their pathological consequences in hypertrophic cardiomyopathy.

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羰基还原酶酶库的构建及其在手性醇制备中的应用

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中国医药工业杂志 2023年第9期54卷 1337-1344页

作者：汤佳伟陈浩张露文张蕊吴海燕奕栋张福利陈少欣中国医药工业研究总院上海医药工业研究院创新药物与制药工艺国家重点实验室上海201203

羰基还原酶(CRED)是手性药物合成中的重要工具酶,已在工业生产中广泛应用。该研究利用多种基因挖掘策略获得了96个不同来源的CRED基因,于大肠埃希菌中克隆、表达,从而构建了CRED酶库1.0,并利用8种不同结构化合物考察了酶库的催化活性。... 详细信息

羰基还原酶(CRED)是手性药物合成中的重要工具酶,已在工业生产中广泛应用。该研究利用多种基因挖掘策略获得了96个不同来源的CRED基因,于大肠埃希菌中克隆、表达,从而构建了CRED酶库1.0,并利用8种不同结构化合物考察了酶库的催化活性。结果表明,不同的酶对不同结构底物的催化活性有显著差别;并且,酶库中包含多个具有高催化活性的CRED,如K21、K42、K38和K73等,可分别高效催化还原乙酰乙酸乙酯、4-氯代乙酰乙酸乙酯、苯乙酮和苯甲酰乙酸乙酯。随后,采用K42和K87高效催化制备了光学手性醇(S)-4-氯-3-羟基丁酸酯(ee>99%),采用K94催化合成(R)-4-氯-3-羟基丁酸酯(ee>99%),时空收率高达800g·(L·d)^(–1)。该研究所构建的CRED酶库1.0为手性药物的工业合成提供了新的选择。

关键词：羰基还原酶酶库重组表达催化活性手性醇

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保留肋间臂神经与不保留肋间臂神经对乳腺癌改良根治术患者的影响

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癌症进展 2024年第15期22卷 1689-1692页

作者：张利军张露文任美田焦作市第二人民医院甲状腺乳腺外科河南焦作4540010

目的探讨保留肋间臂神经与不保留肋间臂神经对乳腺癌改良根治术患者的影响。方法根据手术方式的不同将152例乳腺癌改良根治术患者分为切除组(n=71)和保留组(n=81),切除组患者术中不保留肋间臂神经,保留组患者术中保留肋间臂神经。比较... 详细信息

目的探讨保留肋间臂神经与不保留肋间臂神经对乳腺癌改良根治术患者的影响。方法根据手术方式的不同将152例乳腺癌改良根治术患者分为切除组(n=71)和保留组(n=81),切除组患者术中不保留肋间臂神经,保留组患者术中保留肋间臂神经。比较两组患者的围手术期指标、上肢感觉障碍发生情况、患侧上肢活动情况以及并发症发生情况。结果两组患者手术时间、术中出血量、淋巴结清扫数目、术后引流管拔除时间、术后住院时间及并发症总发生率比较,差异均无统计学意义(P﹥0.05)。术后1周,两组患者患侧上肢外展、后伸、前屈活动度均小于本组术前,保留组患者患侧上肢外展、后伸、前屈活动度均大于切除组,差异均有统计学意义(P﹤0.05)。保留组患者上肢感觉障碍总发生率为9.88%,低于切除组患者的25.35%,差异有统计学意义(P﹤0.05)。结论保留肋间臂神经与不保留肋间臂神经对乳腺癌改良根治术患者围手术期指标及并发症发生率的影响不大,但保留肋间臂神经对患者术后上肢感觉功能影响更小,患者患侧上肢活动度更大。

关键词：乳腺癌改良根治术保留肋间臂神经不保留肋间臂神经

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SnakeGAN: A Universal Vocoder Leveraging DDSP Prior Knowledge and Periodic Inductive Bias

arXiv

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arXiv 2023年

作者： Li, Sipan Liu, Songxiang zhang, luwen Li, Xiang Bian, Yanyao Weng, Chao Wu, Zhiyong Meng, Helen Shenzhen International Graduate School Tsinghua University Shenzhen China AI Lab Tencent Inc Shenzhen China Department of Systems Engineering and Engineering Management The Chinese University of Hong Kong Hong Kong

Generative adversarial network (GAN)-based neural vocoders have been widely used in audio synthesis tasks due to their high generation quality, efficient inference, and small computation footprint. However, it is still challenging to train a universal vocoder which can generalize well to out-of-domain (OOD) scenarios, such as unseen speaking styles, non-speech vocalization, singing, and musical pieces. In this work, we propose SnakeGAN, a GAN-based universal vocoder, which can synthesize high-fidelity audio in various OOD scenarios. SnakeGAN takes a coarse-grained signal generated by a differentiable digital signal processing (DDSP) model as prior knowledge, aiming at recovering high-fidelity waveform from a Mel-spectrogram. We introduce periodic nonlinearities through the Snake activation function and anti-aliased representation into the generator, which further brings desired inductive bias for audio synthesis and significantly improves the extrapolation capacity for universal vocoding in unseen scenarios. To validate the effectiveness of our proposed method, we train SnakeGAN with only speech data and evaluate its performance for various OOD distributions with both subjective and objective metrics. Experimental results show that SnakeGAN significantly outperforms the compared approaches and can generate high-fidelity audio samples including unseen speakers with unseen styles, singing voices, instrumental pieces, and nonverbal vocalization. Copyright © 2023, The Authors. All rights reserved.

关键词： Generative adversarial networks

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SnakeGAN: A Universal Vocoder Leveraging DDSP Prior Knowledge and Periodic Inductive Bias

SnakeGAN: A Universal Vocoder Leveraging DDSP Prior Knowledg...

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IEEE International Conference on Multimedia and Expo (ICME)

作者： Sipan Li Songxiang Liu luwen zhang Xiang Li Yanyao Bian Chao Weng Zhiyong Wu Helen Meng Shenzhen International Graduate School Tsinghua University Shenzhen China AI Lab Tencent Inc Shenzhen China Department of Systems Engineering and Engineering Management The Chinese University of Hong Kong China

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慢性阻塞性肺疾病伴或不伴低氧血症患者血清指标、HOMA-IR与认知功能损伤的关系

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临床医学研究与实践 2025年第14期10卷 87-90页

作者：张露文范丹丹高曼永煤集团总医院河南商丘476600

目的探讨慢性阻塞性肺疾病(COPD)伴或不伴低氧血症患者血清指标、稳态模型评估胰岛素抵抗指数(HOMA-IR)与认知功能损伤的关系。方法选取我院130例COPD患者,根据不吸氧情况下桡动脉血氧分压(PaO2)将其分为A组[51例,伴低氧血症(PaO2<60... 详细信息

目的探讨慢性阻塞性肺疾病(COPD)伴或不伴低氧血症患者血清指标、稳态模型评估胰岛素抵抗指数(HOMA-IR)与认知功能损伤的关系。方法选取我院130例COPD患者,根据不吸氧情况下桡动脉血氧分压(PaO2)将其分为A组[51例,伴低氧血症(PaO2<60 mmHg)]和B组[79例,不伴低氧血症(PaO2≥60 mmHg)];另选取同期60名健康体检者作为对照组。比较三组的单核细胞趋化蛋白-1(MCP-1)、8-异前列腺素F2α(8-iso-PGF2α)、分泌型卷曲相关蛋白5(SFRP5)、瘦素(leptin,LP)水平、HOMA-IR及蒙特利尔认知评估量表(MoCA)评分;采用Pearson相关性分析探究各指标与MoCA评分的关系。结果三组的MoCA评分比较,差异具有统计学意义(P<0.001)。A组的MCP-1、8-iso-PGF2α、LP水平及HOMA-IR明显高于B组和对照组,SFRP5水平明显低于B组和对照组(P<0.05)。Pearson相关性分析显示,MCP-1、8-iso-PGF2α、LP、HOMA-IR与MoCA评分呈显著负相关(r=-0.415、-0.507、-0.281、-0.289,P<0.05);SFRP5与MoCA评分呈显著正相关(r=0.609,P<0.05)。结论COPD伴低氧血症患者多存在认知功能损伤,MCP-1、8-iso-PGF2α、SFRP5、LP、HOMA-IR可参与认知功能损伤过程。

关键词：慢性阻塞性肺疾病低血氧症稳态模型评估胰岛素抵抗指数认知功能

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