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High-throughput sperm screening using one-step RT-qPCR: Improvement and re-evaluation

ANALYTICAL BIOCHEMISTRY 2025年 698卷 115727页

作者： Kubo, Seiji Amai, Keito nakano, fumitaka Tanaka, Jin Niimi, Hideki Univ Toyama Fac Med Dept Clin Lab & Mol Pathol Acad Assembly 2630 Sugitani Toyama 9300194 Japan Ishikawa Prefectural Police Headquarters Forens Sci Lab 1-1 Kuratsuki Kanazawa 9208553 Japan

Sperm identification is crucial in sexual assault cases. While microscopic analysis is the gold standard for sperm detection, it is a laborious procedure even for trained personnel. Reverse transcription-quantitative real-time PCR (RT-qPCR) can enhance the screening by detecting sperm-specific mRNA markers, such as protamine 2 (PRM2). This study aimed to develop a one-step RT-qPCR assay targeting PRM2 mRNA. Our assay was capable of detecting as low as 0.01 mu L of semen with high specificity and demonstrated successful detection of PRM2 mRNA in simulated-case samples. Owing to the simple workflow involved, our assay requires <30 min for RNA extraction and <60 min for RT-qPCR. Our assay enables high-throughput sperm screening and offers a promising strategy for enhancing the workflow of sexual assault cases.

关键词： Semen Sperm PRM2 RT-qPCR

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Electron density derived from dual-energy CT for predicting thrombolytic therapeutic efficacy in patients with pulmonary embolism

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JAPANESE JOURNAL OF RADIOLOGY 2025年 1-9页

作者： Nagano, Hiroaki Takumi, Koji Nagano, Erina nakanosono, Ryota Nakajo, Masatoyo Kamimura, Kiyohisa Nakajo, Masanori Kanzaki, Fumiko Ejima, fumitaka Ayukawa, Takuro Hasegawa, Tomohito nakano, Tsubasa Hirahara, Mitsuho Yoshiura, Takashi Kagoshima Univ Grad Sch Med & Dent Sci Dept Radiol 8-35-1 Sakuragaoka Kagoshima 8908544 Japan Kagoshima Univ Grad Sch Med & Dent Sci Dept Adv Radiol Imaging 8-35-1 Sakuragaoka Kagoshima 8908544 Japan

Purpose To clarify the usefulness of electron density (ED) using dual-energy CT (DECT) parameters for predicting treatment response in patients with pulmonary embolism (PE). Materials and methods The study population comprised 30 patients with PE (49 thrombi) who underwent pretreatment DECT. The study coordinator diagnosed PE using contrast-enhanced CT (CECT) as the gold standard and annotated the location of thrombi on CECT prior to the DECT image analyses. CT attenuation values on conventional 120 kVp, 40 keV, and 70 keV virtual monochromatic (VM) images;effective atomic number;and ED of pretreatment pulmonary thrombi were measured on unenhanced CT. Thrombi were classified into dissolved and residual groups according to the findings of posttreatment follow-up CT. DECT parameters were compared between the two groups using the Mann-Whitney U test. For statistically significant parameters, receiver-operating characteristic (ROC) analysis was used to evaluate their performance for differentiating two groups. Diagnostic accuracy for predicting treatment response in patients with PE was determined by calculating the area under the ROC curve (AUC). Results ED values, CT values on conventional 120 kVp imaging, and those on 70 keV VM imaging were significantly higher in thrombi in the dissolved group than the residual group (p < 0.001, p = 0.012, p = 0.009, respectively). AUC values for predicting dissolution response by ED, conventional 120 kVp imaging, and 70 keV VM imaging (cut-off value, 3.49 x 10(23)/cm(3), 53.4 HU, and 50.7 HU, respectively) were 0.856, 0.744, and 0.755, respectively. AUC was significantly higher for ED than for conventional 120 kVp imaging and 70 keV VM imaging (p = 0.032, p = 0.016). Conclusions ED derived from unenhanced DECT may help predict therapeutic efficacy in patients with PE.

关键词： Pulmonary embolism Dual-energy computed tomography Thrombolytic therapeutic efficacy Electron density

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Time-dependent MR diffusion analysis of functioning and nonfunctioning pituitary adenomas/pituitary neuroendocrine tumors

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JOURNAL OF NEUROIMAGING 2025年第1期35卷 e13254页

作者： Kamimura, Kiyohisa Tokuda, Tomohiro Kamizono, Junki nakano, Tsubasa Hasegawa, Tomohito Nakajo, Masanori Ejima, fumitaka Kanzaki, Fumiko Takumi, Koji Nakajo, Masatoyo Fujio, Shingo Hanaya, Ryosuke Tanimoto, Akihide Iwanaga, Takashi Imai, Hiroshi Feiweier, Thorsten Yoshiura, Takashi Kagoshima Univ Grad Sch Med & Dent Sci Dept Adv Radiol Imaging 8-35-1 Sakuragaoka Kagoshima 8908544 Japan Kagoshima Univ Grad Sch Med & Dent Sci Dept Radiol Kagoshima Japan Kagoshima Univ Grad Sch Med & Dent Sci Dept Neurosurg Kagoshima Japan Kagoshima Univ Grad Sch Med & Dent Sci Dept Pathol Kagoshima Japan Kagoshima Univ Hosp Dept Radiol Technol Kagoshima Japan Siemens Healthcare KK Tokyo Japan Siemens Healthineers AG Erlangen Germany

Background and Purpose Differentiation between functioning and nonfunctioning pituitary adenomas/pituitary neuroendocrine tumors (PAs) is clinically relevant. The goal of this study was to determine the feasibility of using time-dependent diffusion MRI (dMRI) for microstructural characterization of PAs. Methods The study included 54 participants, 24 with functioning PA and 30 with nonfunctioning PA. Time-dependent dMRI of the pituitary gland was performed using an inner field-of-view echo-planar imaging based on 2-dimensional-selective radiofrequency excitations with oscillating gradient and pulsed gradient preparation (effective diffusion time: 7.1 and 36.3 ms) at b-values of 0 and 1000 seconds/mm2. Each tumor had its apparent diffusion coefficients (ADCs) measured at two diffusion times (ADC7.1 ms and ADC36.3 ms), its ADC change (cADC), and relative ADC change. The mean values of diffusion parameters were compared between functioning and nonfunctioning PAs. We compared the diffusion parameters of nonfunctioning PAs with those of each type of hormone-producing PAs. The diagnostic performances of the diffusion parameters were assessed. Results The cADC was significantly higher in functioning PAs than nonfunctioning PAs (p = .0124). The receiver operating characteristic (ROC) curve analysis revealed that cADC (area under the ROC curve [AUC] = .677, p = .017) is effective in distinguishing between functioning and nonfunctioning PAs. The cADC was significantly higher in growth hormone (GH)-producing PAs compared to nonfunctioning PAs (p = .006). The ROC curve analysis indicated that cADC (AUC = .771, p < .001) effectively distinguishes between GH-producing and nonfunctioning PAs. Conclusions The cADC derived from time-dependent dMRI could distinguish between functioning and nonfunctioning PAs, particularly those producing GH.

关键词： diffusion functioning MRI nonfunctioning pituitary adenoma/pituitary neuroendocrine tumor

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Synthesis of a polyester macromonomer via the cobalt-catalyzed alternating copolymerization of propylene oxide and carbon monoxide

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JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY 2004年第18期42卷 4666-4670页

作者： nakano, K fumitaka, K Nozaki, K Univ Tokyo Grad Sch Engn Dept Chem & Biotechnol Bunkyo Ku Tokyo 1138656 Japan

The alternating copolymerization of propylene oxide and carbon monoxide was investigated with cobalt complexes. The NaCo(CO)(4)/amine catalyst system selectively yielded oligo(3-hydroxybutyrate)s bearing a polymerizable crotonate end group, whereas the use of Co-2(CO)(8) as a cobalt source resulted in a smaller concentration of the crotonate end group and a high degree of polymerization. The high selectivity for the oligoesters with the crotonate end group with the NaCo(CO)(4)/amine system was attributed to its more basic reaction condition. (C) 2004 Wiley Periodicals, Inc.

关键词： carbon monoxide copolymerization epoxide macromonomers oligoesters metal-organic catalysts organometallic catalists

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Prefrontal Cortical Involvement in Young Infants' Analysis of Novelty

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CEREBRAL CORTEX 2009年第2期19卷 455-463页

作者： nakano, Tamami Watanabe, Hama Homae, fumitaka Taga, Gentaro Univ Tokyo Grad Sch Educ Dept Phys & Hlth Educ Bunkyo Ku Tokyo 1130033 Japan Japan Sci & Technol Agcy Japan Soc Promot Sci Kawaguchi Saitama 3320012 Japan Japan Sci & Technol Agcy CREST Kawaguchi Saitama 3320012 Japan

Our knowledge of infant perception and cognition is primarily based on habituation and dishabituation, but the underlying neural mechanisms for these processes per se remain unclear. It has been argued that habituation is related to building internal representations of repeated stimuli in the central nervous system, whereas dishabituation is related to an increased attention to novel items and events. This leads to a hypothesis that a distributed network including the sensory, association and prefrontal cortical regions of young infants is involved in those processes, in contrast with the classical developmental view that onset of the functioning of the prefrontal cortex is delayed. Here we examined the time evolution of spatio-temporal hemodynamic responses related to the auditory habituation and dishabituation in the temporal and prefrontal regions of 3-month-old infants by using multichannel near-infrared spectroscopy. We found that the temporal regions remained activated by repetitive auditory stimuli;however, the prefrontal regions exhibited phasic activation in relation to novel stimuli. The dissociated activation pattern between the temporal and prefrontal regions suggests that distinct cortical regions play distinct functional roles in auditory habituation and dishabituation, and that the prefrontal cortex is involved in perceiving invariance or novelty of the immediate environment in early infancy.

关键词： dishabituation habituation infant NIRS novelty

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Functional development in the infant brain for auditory pitch processing

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HUMAN BRAIN MAPPING 2012年第3期33卷 596-608页

作者： Homae, fumitaka Watanabe, Hama nakano, Tamami Taga, Gentaro Tokyo Metropolitan Univ Dept Language Sci Hachioji Tokyo 1920397 Japan Univ Tokyo Grad Sch Educ Tokyo Japan Juntendo Univ Dept Neurophysiol Tokyo Japan Japan Sci & Technol Agcy CREST Kawaguchi Saitama Japan

Understanding how the developing brain processes auditory information is a critical step toward the clarification of infants' perception of speech and music. We have reported that the infant brain perceives pitch information in speech sounds. Here, we used multichannel near-infrared spectroscopy to examine whether the infant brain is sensitive to information of pitch changes in auditory sequences. Three types of auditory sequences with distinct temporal structures of pitch changes were presented to 3- and 6-month-old infants: a long condition of 12 successive tones constructing a chromatic scale (600 ms), a short condition of four successive tones constructing a chromatic scale (200 ms), and a random condition of random tone sequences (50 ms per tone). The difference among the conditions was only in the sequential order of the tones, which causes pitch changes between the successive tones. We found that the bilateral temporal regions of both ages of infants showed significant activation under the three conditions. The stimulus-dependent activation was observed in the right temporoparietal region of the both infant groups;the 3- and 6-month-old infants showed the most prominent activation under the random and short conditions, respectively. Our findings indicate that the infant brain, which shows functional differentiation and lateralization in auditory-related areas, is capable of responding to more than single tones of pitch information. These results suggest that the right temporoparietal region of the infants increases sensitivity to auditory sequences, which have temporal structures similar to those of syllables in speech sounds, in the course of development. Hum Brain Mapp, 2012. (C) 2011 Wiley Periodicals, Inc.

关键词： music neuroimaging NIRS speech perception temporal cortex

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Functional activation in diverse regions of the developing brain of human infants

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NEUROIMAGE 2008年第2期43卷 346-357页

作者： Watanabe, Hama Homae, fumitaka nakano, Tamami Taga, Gentaro Univ Tokyo Dept Phys & Hlth Educ Grad Sch Educ Bunkyo Ku Tokyo 1130033 Japan Japan Sci & Technol Agcy CREST Kanagawa Japan

To understand the functional organization of the human cortex during the early postnatal period, we performed neuroimaging Studies for visual perception in awake 3-month-old infants. Cortical hemodynamic responses to 2 different video images, moving mobile objects and black-and-white checkerboard pattern reversals, were observed using Multichannel near-infrared spectroscopy (NIRS). Although a focal region of the occipital cortex was equally activated by both stimuli, the occipitotemporal region was activated only by the mobile objects. A possible explanation of the result is that the former and the latter regions are involved in the primary processing Of Visual stimuli and perception of objects with complex visual features, respectively. Furthermore, the prefrontal region was distinctly activated by the mobile objects. These results suggest that the early sensory region and the higher sensory/association and prefrontal regions are functionally differentiated by 3 months of age and that diverse regions of the cortex including the prefrontal region function during perception of visual events. (c) 2008 Elsevier Inc. All rights reserved.

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Prosodic processing in the developing brain

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NEUROSCIENCE RESEARCH 2007年第1期59卷 29-39页

作者： Homae, fumitaka Watanabe, Hama nakano, Tamami Taga, Gentaro Univ Tokyo Grad Sch Educ Bunkyo Ku Tokyo 113 Japan Japan Sci & Technol Agcy CREST Saitama 332 Japan

Speech prosody is considered to be one of the most important sources of information for infants in acquiring their native language. Using multi-channel near-infrared spectroscopy in 10-month-old infants, we examined cortical activation when normal and flattened speech sounds were presented to the infants. The flattened speech sound was generated by eliminating changes in the pitch contours of the original utterance. We found bilateral activation under both speech conditions. In a direct comparison between the two conditions, the right temporal and temporoparietal regions, and bilateral prefrontal regions showed more prominent activation in response to flattened speech than to normal speech. These results demonstrate that the unfamiliar pitch contours of flattened speech induce additional processing in the cortical regions of 10-month-old infants, suggesting that 10-month-old infants already have neural mechanisms for the processing of at least a part of the prosodic structures in their native language. To investigate developmental changes in cortical activation patterns, we compared the present results with those of our previous study using the same paradigm with 3-month-old infants. We propose that speech processing in the infant brain develops from analyzing pitch information per se, to comparing and integrating information in input speech sounds with acquired prosodic structures. (C) 2007 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

关键词： infant language acquisition NIRS pitch prosody speech perception

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Genome Mining Reveals Two Novel Bacterial Sesquiterpene Cyclases: (-)-Germacradien-4-ol and (-)-epi-α-Bisabolol Synthases from Streptomyces citricolor

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CHEMBIOCHEM 2011年第15期12卷 2271-2275页

作者： nakano, Chiaki Kudo, fumitaka Eguchi, Tadashi Ohnishi, Yasuo Univ Tokyo Dept Biotechnol Grad Sch Agr & Life Sci Bunkyo Ku Tokyo 1138657 Japan Tokyo Inst Technol Dept Chem Meguro Ku Tokyo 1528551 Japan Tokyo Inst Technol Dept Chem & Mat Sci Meguro Ku Tokyo 1528551 Japan

Now found in bacteria: An increasing number of genome sequences indicate that bacteria possess a variety of terpenoid cyclase genes. The characterization of two sesquiterpene cyclase (SC) genes found in the draft genome sequence of Streptomyces citricolor is described here. Our study strongly supports the idea that genome mining is a useful approach in revealing the terpenoid diversity in bacteria.

关键词： cyclization genome mining sesquiterpene cyclases Streptomyces citricolor terpenoids

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A NOVEL APPROACH TO DETECTING POSTPARTUM HEMORRHAGE USING CONTRAST-ENHANCED ULTRASOUND

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ULTRASOUND IN MEDICINE AND BIOLOGY 2017年第3期43卷 615-620页

作者： Imai, Kenji Kotani, Tomomi Tsuda, Hiroyuki nakano, Tomoko Hirakawa, Akihiro Kikkawa, fumitaka Nagoya Univ Grad Sch Med Dept Obstet & Gynecol Nagoya Aichi Japan Nagoya Univ Grad Sch Med Ctr Adv Med & Clin Res Biostat Sect Nagoya Aichi Japan

The aim of this study was to estimate the efficacy of contrast-enhanced ultrasound (CEUS) in detecting postpartum hemorrhage (PPH) after cesarean section. This is the first study of CEUS in obstetric hemorrhage. A total of 37 patients, operated at Nagoya University Hospital, underwent CEUS. We evaluated the findings of CEUS, which were qualitatively defined as positive when pooling or leakage of contrast agent was observed in the uterine cavity, by measuring the amount of bleeding during the first 4 h after cesarean section. The time-intensity curve patterns of leaked contrast agents were also analyzed for quantitative prediction of the amount of blood loss. Significant differences between the excessive hemorrhage (N = 7) and non-excessive hemorrhage groups ( N = 30) were noted in the occurrence of positive CEUS (p = 0.011). Additionally, mean postpartum blood loss markedly increased in patients with a positive CEUS ( p = 0.002). From a quantitative perspective, the time until leakage of contrast agents was detected correlated with the amount of bleeding, but the other characteristics of the time- intensity curve pattern did not provide valuable information. In conclusion, CEUS, which enables bedside assessment and rapid diagnosis, is a promising strategy for the detection of PPH. (C) 2016 World Federation for Ultrasound in Medicine & Biology.

关键词： Postpartum hemorrhage Contrast-enhanced ultrasound Perflubutane Cesarean section Obstetrics

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