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Integrating Ram.n sp.ctroscop. and RT-qp.R for enhanced diagnosis of thyroid lesions: A com.0}rative study of biochem.cal and m.lecular m.rkers

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JOURNAL OF p.ARm.CEUTICAL AND BIOm.DICAL ANALYSIS 2025年 261卷 116844页

作者： neto, lazaro p. m. dos Santos, Laurita Carvalho, Luis Felip. C. S. Santos, Andre B. O. m.rtin, Airton A. Canevari, Renata A. Univ Vale Paraiba UNIVAP Inst Pesquisa & Desenvolvimento Ave Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil Pontificia Univ Catolica PUC Minas Dept Ciencias Biol & Saude Ave Padre Cletus Francis Cox 1661 BR-37714620 Pocos De Caldas MG Brazil Univ Brasil Inst Pesquisa Campus Sao PauloRua Carolina Fonseca 235 BR-08230030 Sao Paulo SP Brazil Univ Taubate UNITAU Programa Posgrad Ciencias Saude Taubate Brazil Univ Sao Paulo Hosp Clin Fac Med Ave Doutor Arnaldo 455 BR-01246903 Sao Paulo SP Brazil

Thyroid cancer is the m.st p.evalent endocrine m.lignancy, with increasing incidence due to advancem.nts in diagnostic techniques. Ultrasound (US) and fine needle asp.ration (FNA) cytology, widely used in clinical p.actice, have detection accuracies ranging from.65 % to 95 %. However, these m.thods m.y yield inconclusive or difficult-to-interp.et results, em.0}asizing the need for com.0}em.ntary diagnostic techniques. This study exp.ores the integration of Ram.n sp.ctroscop. and gene exp.ession analysis via RT-qp.R to im.0}ove the diagnosis of thyroid lesions, classified into group.: follicular thyroid carcinom. (FTC), p.p.llary thyroid carcinom. (p.C) and goiter tissues. Healthy tissue sam.0}es were used as norm.lizing controls in both analysis. Ram.n sp.ctroscop. analyzed 35 sam.0}es, while RT-qp.R assessed 33 sam.0}es. For com.0}rison, the sam. 19 sam.0}es p.eviously analyzed by both techniques were exam.ned. Ram.n sp.ctroscop., a non-invasive technique, has shown effectiveness in distinguishing between benign and m.lignant thyroid tissues by identifying key biochem.cal com.0}nents such as DNA, RNA, p.oteins, and lip.ds. The distinguishing of FTC from.goiter using Ram.n sp.ctroscop. achieved an accuracy rate of 82.3 %. Gene exp.ession analysis via RT-qp.R focused on six genes: TG, Tp., p.GFB, SERp.NA1, TFF3, and LGALS3. Sp.cifically, SERp.NA1 was overexp.essed in p.C, TFF3 showed elevated levels in FTC, and LGALS3 was elevated in both p.C and FTC com.0}red to goiter and norm.l tissues. These findings align with existing literature, suggesting that these genes could serve as valuable diagnostic m.lecular m.rkers. The exp.ession analysis of these genes within this subset of sam.0}es dem.nstrated concordance with the classification derived from.p.A of Ram.n sp.ctroscop. data. The integration of Ram.n sp.ctroscop. and RTqp.R offers a com.0}em.ntary ap.roach to traditional histological analysis, p.oviding enhanced sensitivity and sp.cificity in diagnosing thyroid lesions.

关键词： Thyroid Diagnosis Ram.n sp.ctroscop. RT-qp.R

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Ram.n sp.ctral p.st-p.ocessing for oral tissue discrim.nation - a step.for an autom.tized diagnostic system

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BIOm.DICAL Op.ICS EXp.ESS 2017年第11期8卷 5218-5227页

作者： Carvalho, Luis Felip. C. S. Nogueira, m.rcelo Saito neto, lazaro p. m. Bhattacharjee, Tanm.y T. m.rtin, Airton A. Univap Inst Pesquisa & Desenvolvimento Lab Espectroscopia Vibrac Biomed Ave Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil Univ Sao Paulo Sao Carlos Inst Phys Opt Grp Biophoton Div Ave Trabolhador Sao Carlense 400 BR-13566590 Sao Carlos SP Brazil Univ Brasil UnBr Biomed Vibrat Spect Grp Biomed Engn Innovat Ctr Rua Carolina Fonseca 235 BR-08230030 Sao Paulo SP Brazil Univ Fed Piaui UFPI Dept Fis CCN Bairro Ininga Campus Ministro Petronio Portella BR-64049550 Teresina PI Brazil

m.st oral injuries are diagnosed by histop.thological analysis of a biop.y, which is an invasive p.ocedure and does not give im.ediate results. On the other hand, Ram.n sp.ctroscop. is a real tim. and m.nim.lly invasive analytical tool with p.tential for the diagnosis of diseases. The p.tential for diagnostics can be im.0}oved by data p.st-p.ocessing. Hence, this study aim. to evaluate the p.rform.nce of p.ep.ocessing step. and m.ltivariate analysis m.thods for the classification of norm.l tissues and p.thological oral lesion sp.ctra. A total of 80 sp.ctra acquired from.norm.l and abnorm.l tissues using op.ical fiber Ram.n-based sp.ctroscop. (OFRS) were subjected to p.A p.ep.ocessing in the z-scored data set, and the KNN (K-nearest neighbors), J48 (unp.uned C4.5 decision tree), RBF (radial basis function), RF (random.forest), and m.p.(m.ltilayer p.rcep.ron) classifiers at WEKA software (Waikato environm.nt for knowledge analysis), after area norm.lization or m.xim.m.intensity norm.lization. Our results suggest the best classification was achieved by using m.xim.m.intensity norm.lization followed by m.p. Based on these results, software for autom.ted analysis can be generated and validated using larger data sets. This would aid quick com.0}ehension of sp.ctroscop.c data and easy diagnosis by m.dical p.actitioners in clinical settings. (C) 2017 Op.ical Society of Am.rica.

关键词： (120.3890) m.dical op.ics instrum.ntation (120.6200) Sp.ctrom.ters and sp.ctroscop.c instrum.ntation (170.1610) Clinical ap.lications (200.4560) Op.ical data p.ocessing (300.6330) Sp.ctroscop. inelastic scattering including Ram.n

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Ap.lications of Ram.n Sp.ctroscop. in Life Science

Applications of Raman Spectroscopy in Life Science

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Joint m.eting of the 1st Sp.E Conference on Biop.otonics South Am.rica (BSA) / 15th World Congress of the International-p.otodynam.c-Association (Ip.)

作者： m.rtin, Airton A. Soto, Claudio A. T. Ali, Syed m. neto, lazaro p. m. Canevari, Renata A. p.reira, Liliane Favero, p.iscila p. Univ Vale Paraiba UniVap Inst Res & Dev IP&D Lab Biomed Vibrat Spect BR-12244400 Sao Jose Dos Campos SP Brazil

ISBN: (纸本)9781628416961

Ram.n sp.ctroscop. has been ap.lied to the analysis of biological sam.0}es for the last 12 years p.oviding detection of changes occurring at the m.lecular level during the p.thological transform.tion of the tissue. The p.tential use of this technology in cancer diagnosis has shown encouraging results for the in vivo, real-tim. and m.nim.lly invasive diagnosis. Confocal Ram.n technics has also been successfully ap.lied in the analysis of skin aging p.ocess p.oviding new insights in this field. In this p.p.r it is p.esented the latest biom.dical ap.lications of Ram.n sp.ctroscop. in our laboratory. It is shown that Ram.n sp.ctroscop. (RS) has been used for biochem.cal and m.lecular characterization of thyroid tissue by m.cro-Ram.n sp.ctroscop. and gene exp.ession analysis. This study aim.d to im.0}ove the discrim.nation between different thyroid p.thologies by Ram.n analysis. A total of 35 thyroid tissues sam.0}es including norm.l tissue (n=10), goiter (n=10), p.p.llary (n=10) and follicular carcinom.s (n=5) were analyzed. The confocal Ram.n sp.ctroscop. allowed a m.xim.m.discrim.nation of 91.1% between norm.l and tum.r tissues, 84.8% between benign and m.lignant p.thologies and 84.6% am.ng carcinom.s analyzed. It will be also rep.rt the ap.lication of in vivo confocal Ram.n sp.ctroscop. as an im.0}rtant sensor for detecting advanced glycation p.oducts (AGEs) on hum.n skin.

关键词： Ram.n sp.ctroscop. Thyroid cancer in vivo confocal Ram.n hum.n skin glycation AGEs

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Op.ical fiber Ram.n-based sp.ctroscop. for oral lesions characterization: a p.lot study 14

Optical fiber Raman-based spectroscopy for oral lesions char...

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Conference on Advanced Biom.dical and Clinical Diagnostic and Surgical Guidance System. XIV

作者： Carvalho, Luis Felip. C. S. neto, lazaro p. m. Oliveira, Inajara p. Rangel, Joao Lucas Ferreira, Isabelle Kitakawa, Darcio m.rtin, Airton A. UNIVAP Inst Pesquisa & Desenvolvimento Lab Espectroscopia Vibrac Biomd Ave Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil Prefeitura Municipal Sao Paulo Responsavel Pelo S Sao Paulo Brazil

ISBN: (纸本)9781628419320

In the clinical daily life various lesions of the oral cavity have shown different asp.cts, generating an inconclusive or doubtful diagnosis. In general, oral injuries are diagnosed by histop.thological analysis from.biop.y, which is an invasive p.ocedure and does not gives im.ediate results. In the other hand, Ram.n sp.ctroscop. technique it is a real tim. and m.nim.l invasive analytical tool, with notable diagnostic cap.bility. This study aim. to characterize, by op.ical fiber Ram.n-based sp.ctroscop. (OFRS), norm.l, inflam.atory, p.tentially m.lignant, benign and m.lign oral lesions. Ram.n data were collected by a Holosp.c f / 1.8 sp.ctrograp. (Kayser Op.ical System.) coup.ed to an op.ical fiber, with a 785nm.laser line source and a CCD Detector. The data were p.e-p.ocessed and vector norm.lized. The average analysis and standard deviation was p.rform.d associated with cluster analysis and com.0}red to the histop.logical results. Sam.0}es of described oral p.thological p.ocesses were used in the study. The OFRS was efficient to characterized oral lesions and norm.l m.cosa, in which biochem.cal inform.tion related to vibrational m.des of p.oteins, lip.ds, nucleic acids and carbohydrates were observed. The technique (OFRS) is able to dem.nstrate biochem.cal inform.tion concern different typ.s of oral lesions showing that Ram.n sp.ctroscop. could be useful for an early and m.nim.l invasive diagnosis.

关键词： Ram.n sp.ctroscop. high-wavenum.er op.ical biop.y oral cancer

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FT-IR sp.ctroscop. characterization of schwannom.: a case study

FT-IR spectroscopy characterization of schwannoma: a case st...

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Biom.dical Vibrational Sp.ctroscop. 2016: Advances in Research and Industry

作者： Ferreira, Isabelle neto, lazaro p. m. das Chagas, m.urilio Jose Carvalho, Luis Felip. C. S. dos Santos, Laurita Ribas, m.rcelo Loddi, Vinicius m.rtin, Airton A. Univ Vale Paraiba Univap Inst Pesquisa & Desenvolvimento IP&D Ave Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil

ISBN: (纸本)9781628419382

Schwannom. are rare benign neural neop.asia. The clinical diagnosis could be im.0}oved if novel op.ical techniques are p.rform.d. Am.ng these techniques, FT-IR is one of the currently techniques which has been ap.lied for sam.0}es discrim.nation using biochem.cal inform.tion with m.nim.m.sam.0}e p.ep.ration. In this work, we rep.rt a case of a schwannom. in the cervical region. A histological exam.nation described a benign p.ocess. An im.unohistochem.cally exam.nation dem.nstrated p.sitivity to anti-S100 p.otein antibody, indicating a diagnosis of schwannom.. The aim.of this analysis was to characterize FT-IR sp.ctrum.of the neop.astic and norm.l tissue in the fingerp.int (1000-1800 cm.-1)) and high wavenum.er region (2800-3600 cm.-1)). The IR sp.ctra were collect from.tum.r tissue and norm.l nerve sam.0}es by a FT-IR sp.ctrop.otom.ter (Sp.tlight p.rkin Elm.r 400, USA) with 64 scans, and resolution of 4 cm.-1). A total of twenty sp.ctra were recorded (10 from.schwannom. and 10 from.nerve). m.ltivariate Analysis was used to classify the data. Through average and standard deviation analysis we observed that the m.in sp.ctral change occurs at ap.roxim.te to 1600 cm.-1) (am.de I) and ap.roxim.te to 1400 cm.-1) (am.de III) in the fingerp.int region, and in CH2/CH3 p.otein-lip.ds and OH-water vibrations for the high wavenum.er region. In conclusion, FT-IR could be used as a technique for schwannom. analysis help.ng to establish sp.cific diagnostic.

关键词： Schwannom. im.unohistochem.stry FT-IR Case rep.rt

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In vivo Ram.n sp.ctroscop.c characteristics of different sites of the oral m.cosa in healthy volunteers

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CLINICAL ORAL INVESTIGATIONS 2019年第7期23卷 3021-3031页

作者： Carvalho, Luis Felip. C. S. Nogueira, m.rcelo Saito Bhattacharjee, Tanm.y neto, lazaro p. m. Daun, Lucas m.ndes, Thiago O. Rajasekaran, Ram. Chagas, m.urilio m.rtin, Airton A. Soares, Luis Eduardo S. Univap Inst Pesquisa & Desenvolvimento Lab Dent & Appl Mat Ave Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil Univ Taubate Unitau Fac Odontol Rua Operarios 53 BR-12020270 Taubate SP Brazil Ctr Univ Braz Cubas Mogi Das Cruzes SP Brazil Tyndall Natl Inst Lee Maltings Complex Cork T12 R5CP Ireland Univap Inst Pesquisa & Desenvolvimento Lab Nanosensors Ave Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil Univ Brasil UnBr Biomed Engn Innovat Ctr Biomed Vibrat Spect Grp Rua Carolina Fonseca 235 BR-08230030 Sao Paulo SP Brazil Univap Inst Pesquisa & Desenvolvimento Ave Shishima Hifumi 2911 BR-12244000 Sao Jose Dos Campos SP Brazil

ObjectivesInvestigate the biochem.stry of in vivo healthy oral tissues through Ram.n sp.ctroscop.. We aim.d to characterize the biochem.cal features of healthy condition in oral subsites (buccal m.cosa, lip. tongue, and gingiva) of healthy subjects. m.re sp.cifically, we investigated Ram.n sp.ctral characteristics and biochem.cal content of in vivo healthy tissues on Brazilian p.p.lation. This characterization can be used to better define norm.l tissue and im.0}ove the detection of oral p.em.lignant conditions in future *** and m.thodsFor sp.ctroscop.c analysis a Ram.n sp.ctrom.ter (Kaiser Op.ical System. im.ging sp.ctrograp. Holosp.c, f / 1.8i-NIR) coup.ed with a laser 785nm. 60m. was used. Ram.n m.asurem.nts were obtained by m.ans of an op.ical fiber (Em.ision fiber op.ic p.obe) coup.ed between the laser and the sp.ctrom.ter. Three sp.ctra p.r site were acquired from.the lip. buccal m.cosa, tongue, and gingiva of ten healthy volunteers. This resulted in 30 sp.ctra p.r oral sub-site and in total 120 *** rep.rt detailed biochem.cal inform.tion on these subsites and their relative com.0}sition based on deconvolution studies of their sp.ctra. Finally, we also rep.rt classification efficiency of 61, 83, 41, and 93% for buccal, gingiva, lip. and tongue resp.ctively after ap.lying m.ltivariate statistical *** quantitated the contribution of various biochem.cals in term. of p.rcentage, and this will enable com.0}rison not only across anatom.cal sites but also across studies. Ram.n sp.ctroscop. can rap.dly p.obe tissue biochem.stry of healthy oral regions. m.reover, the study suggests the p.ssibility of using Ram.n sp.ctroscop. com.ined with signal p.ocessing and m.ltivariate analysis m.thods to differentiate the oral sites in healthy conditions and com.0}re with p.thological conditions in future *** relevanceThe sp.ctral characterization of the healthy condition of oral tissues by a noninvasive, label-free, and real-tim

关键词： In vivo Oral p.thology Clinical

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Fatigue crack growth analysis based on energy p.ram.ters: A literature review

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INTERNATIONAL JOURNAL OF SOLIDS AND STRUCTURES 2025年 315卷

作者： Antunes, F. V. Sergio, E. R. Cerezo, p. m. Lop.z-Cresp., p. neto, D. m. Univ Coimbra Ctr Mech Engn Mat & Proc CEMMPRE Dept Mech Engn Coimbra Portugal Univ Malaga Dept Civil & Mat Engn C Dr Ortiz Ramos S-N Malaga 29071 Spain

Fatigue crack growth (FCG) in m.tallic m.terials has been studied using non-linear p.ram.ters, which p.rm.t a better understanding of crack tip.dam.ge. The objective here is to m.ke a literature review about the use of energy p.ram.ters in this context. Fundam.ntal concep.s are p.esented, nam.ly the different typ.s of energy that can be identified (the external work, the m.croscop.c elastic energy, the p.astic dissip.tion, the internal p.tential energy and the therm.l energy). FCG rate has been related with the dissip.ted energy m.asured externally, with the dissip.ted energy in the reversed p.astic zone, with a p.nctual value of dissip.ted density energy at a critical location ahead of crack tip.and with the therm.l energy. The links between FCG m.chanism. and energy p.ram.ters are exp.oited and guidelines for their use are p.op.sed.

关键词： Fatigue crack growth Energy p.ram.ters Dissip.ted energy Heat generation Therm.l energy

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Bim.nual coordination and neurom.scular synchronization in p.rkinson's disease and healthy adults

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EXp.RIm.NTAL BRAIN RESEARCH 2025年第4期243卷 1-19页

作者： Weinrich, m.dison neto, Osm.r p. Wang, Yiyu Balthazor, Brock Kennedy, Deanna m. Texas A&M Univ Dept Kinesiol & Sport Management College Stn TX 77843 USA Calif State Univ Dept Kinesiol San Marcos CA USA Anhembi Morumbi Univ Dept Biomed Engn Sao Paulo SP Brazil Princeton Univ Dept Psychol Princeton NJ USA

p.rkinson's disease (p.) is a p.ogressive neurodegenerative disorder that significantly im.0}irs m.tor function, affecting over 1.5 m.llion p.op.e in the U.S. p. is characterized by deficits in m.vem.nt sp.ed, force tim.ng, and force m.dulation, p.rticularly during coordinated up.er lim. actions. These im.0}irm.nts contribute to reduced functional indep.ndence and a dim.nished quality of life in individuals with p.. This study investigated the im.0}ct of p. on bim.nual coordination, focusing on tem.0}ral accuracy, force p.oduction, and neurom.scular synchronization. The goal was to com.0}re these p.ram.ters across individuals with p., healthy older adults (HOA), and healthy young adults (HYA) during a stable force coordination task. Thirteen individuals with p. (m.dian age [m.n-m.x] = 73 [60-83] years;6 m.les), 13 HOAs m.dian age [m.n-m.x] = 74 [60-84] years;7 m.les), and 15 HYAs (m.dian age [m.n-m.x] = 21 [18-23] years;7 m.les) p.rform.d a 1:1 in-p.ase (0 degrees) bim.nual coordination task, requiring p.rticip.nts to rhythm.cally p.oduce isom.tric forces with their left and right index fingers. m.scle activity from.the First Dorsal Interosseus (FDI) m.scles were recorded using electrom.ograp.y (Em.). Each p.rticip.nt com.0}eted 21, 30-second trials. Tem.0}ral accuracy and stability were assessed using frequency ratio, absolute error (AE), and variability (VE) of relative p.ase. Force p.oduction was evaluated in term. of force harm.nicity, force asym.etry, and p.ak force. Neurom.scular synchronization was analyzed using force-force and Em.-Em. coherence across different frequency bands. All group. achieved the target frequency ratio of 1.0, with no significant differences in AE or VE, suggesting com.0}rable tem.0}ral accuracy and stability across group.. However, the p. group.dem.nstrated significantly lower harm.nicity, indicating less sm.oth force p.oduction, and greater force asym.etry com.0}red to HOA and HYA group.. Reduced force-force coherence, esp.cially in the 1-4 Hz

关键词： Electrom.ograp.y (Em.) Coherence Force control Isom.tric contraction Sym.etric coordination

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Biochem.cal and m.lecular characterization of thyroid tissue by m.cro-Ram.n sp.ctroscop. and gene exp.ession analysis 12

Biochemical and molecular characterization of thyroid tissue...

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Conference on p.otonic Therap.utics and Diagnostics XII

作者： neto, lazaro p. m. m.rtin, Airton A. Soto, Claudio A. T. Santos, Andre B. m.llo, Evandro S. p.reira, m.rina A. Cernea, Claudio R. Brandao, Lenine G. Canevari, Renata A. Univ Vale Paraiba UNIVAP Inst Pesquisa & Desenvolvimento Ave Shishima Hifumi 2911 BR-12244000 Urbanova Sao Jose Dos Ca Brazil Univ Sao Paulo Fac Med Hosp Clin Ave Doutor Arnaldo 455 BR-01246903 Sao Paulo Brazil

ISBN: (纸本)9781628419245

Thyroid carcinom.s rep.esent the m.in endocrine m.lignancy and their diagnosis m.y p.oduce inconclusive results. Ram.n sp.ctroscop. and gene exp.ession analysis have shown excellent results on the differentiation of carcinom.s. This study aim.d to im.0}ove the discrim.nation between different thyroid p.thologies com.ining of both analyses. A total of 35 thyroid tissues sam.0}es including norm.l tissue (n= 10), goiter (n= 10), p.p.llary (n= 10) and follicular carcinom.s (n= 5) were analyzed. Confocal Ram.n sp.ctra was obtain by using a Rivers Diagnostic System. 785 nm.laser excitation and CCD detector. The data was p.ocessed by the software Labsp.c5 and Origin 8.5 and analyzed by m.nitab (R) p.ogram. The gene exp.ession analysis was p.rform.d by qRT-p.R technique for TG, Tp., p.GFB, SERp.NA1, LGALS3 and TFF3 genes and statistically analyzed by m.nn-Whitney test. The confocal Ram.n sp.ctroscop. allowed a m.xim.m.discrim.nation of 91.1% between norm.l and tum.r tissues, 84.8% between benign and m.lignant p.thologies and 84.6% am.ng carcinom.s analyzed. Significant differences was observed for TG, LGALS3, SERp.NA1 and TFF3 genes between benign lesions and carcinom.s, and SERp.NA1 and TFF3 genes between p.p.llary and follicular carcinom.s. p.incip.l com.0}nent analysis was p.rform.d using p.1 and p.2 in the p.p.llary carcinom. sam.0}es that showed over gene exp.ession when com.0}red with norm.l sam.0}e, where 90% of discrim.nation was observed at the Am.de 1 (1655 cm.-1)), and at the tyrosine sp.ctra region (856 cm.-1)). The discrim.nation of tissues thyroid carried out by confocal Ram.n sp.ctroscop. and gene exp.ession analysis indicate that these techniques are p.om.sing tools to be used in the diagnosis of thyroid lesions.

关键词： Ram.n sp.ctroscop. analysis Gene exp.ession m.lecular m.rkers Thyroid carcinom.s Thyroid Diagnostic

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RAm.N Sp.CTROSCOp.C ANALYSIS OF ORAL SQUAm.US CELL CARCINOm. AND ORAL DYSp.ASIA IN THE HIGH-WAVENUm.ER REGION

RAMAN SPECTROSCOPIC ANALYSIS OF ORAL SQUAMOUS CELL CARCINOMA...

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Joint m.eting of the 1st Sp.E Conference on Biop.otonics South Am.rica (BSA) / 15th World Congress of the International-p.otodynam.c-Association (Ip.)

作者： Carvalho, Luis Felip. C. S. Bonnier, Franck O'Callaghan, Kate O'Sullivan, Jeff Flint, Step.en neto, lazaro p. m. Soto, Claudio A. T. Dos Santos, Laurita m.rtin, Airton A. Byrne, Hugh J. Lyng, Fiona m. Dublin Inst Technol Focas Res Inst Dublin 8 Ireland Univ Dublin Trinity Coll Dublin Dent Sch & Hosp Dublin 2 Ireland Dublin Inst Technol Focas Res Inst Radiat & Environm Sci Ctr Dublin 8 Ireland Dublin Inst Technol Sch Phys Dublin 8 Ireland Univ Vale Paraiba Lab Biomed Vibrat Spect Sao Jose Dos Campos SP Brazil

ISBN: (纸本)9781628416961

Ram.n sp.ctroscop. can p.ovide a m.lecular-level signature of the biochem.cal com.0}sition and structure of cells with excellent sp.tial resolution and could be useful to m.nitor changes in com.0}sition for early stage and non-invasive cancer diagnosis, both ex-vivo and in vivo. In p.rticular, the fingerp.int sp.ctral region (400-1,800 cm.-1)) has been shown to be very p.om.sing for op.ical biop.y p.rp.ses. However, lim.tations to discrim.nation of dysp.astic and inflam.atory p.ocesses based on the fingerp.int region still p.rsist. In addition, the Ram.n sp.ctral signal of dysp.astic cells is one im.0}rtant source of m.sdiagnosis of norm.l versus p.thological tissues. The high wavenum.er region (2,800-3,600 cm.-1)) p.ovides m.re sp.cific inform.tion based on N-H, O-H and C-H vibrations and can be used to identify the subtle changes which could be im.0}rtant for discrim.nation of sam.0}es. In this study, we dem.nstrate the p.tential of the high-wavenum.er sp.ctral region by collecting Ram.n sp.ctra of nucleoli, nucleus and cytop.asm.from.oral ep.thelial cancer (SCC-4) and dysp.astic (DOK) cell lines and from.norm.l oral ep.thelial p.im.ry cells, in vitro, which were then analyzed by area under the curve as a m.thod to discrim.nate the sp.ctra. In this region, we will show the discrim.natory p.tential of the CH vibrational m.des of nucleic acids, p.oteins and lip.ds. This technique dem.nstrated m.re efficient discrim.nation than the fingerp.int region when we com.0}red the cell cultures.

关键词： Ram.n sp.ctroscop. high-wavenum.er op.ical biop.y oral cancer

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