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4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE)

作者： Guo Bei Song Yanzhai Guo xuwen Wang Wenping Ding ning Beijing Agr Univ Dept Biotechnol Beijing Peoples R China

ISBN: (纸本)9781424447138

The representative individuals, CK (non-transgenic), 121 (with pBI121 vector), 1285 (with pGSA1285 vector), T4 (with gene of trehalase, TRE), S1 (with gene of trehalose -6 -phosphate synthase, TPS), P3 (with gene of trehalose -6 -phosphate phosphatase, TPP), a3 (with anti-TRE gene), i2 (with RNAi-TRE gene), were selected for identification of drought resistance, and the Malondialdehyde (MDA), relative conductivity have been measured after water-stress during this experiment. The results showed that the transgenic tobaccos with anti-TRE and RNAiTRE grew better than other individuals under water-stress conditions;conductivity of i2 was lower than other plants. Meantime, i2 has performance of some special features. These indicate that through application the antisense RNA and RNA interference technology to trehalase gene, it is possible to make the receptor trehalose metabolism changes. The inhibition of gene expression of trehalase can led to the accumulation of trehalose content and the enhancement of drought resistance in the transgenic tobaccos.

关键词： trehalase geng expression transgenic tobacco drought resistance

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Attribute Reduction Method Using Adaptive Genetic Algorithm and Particle Swarm Optimization 2021

Attribute Reduction Method Using Adaptive Genetic Algorithm ...

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7th International Conference on Computing and Artificial Intelligence, ICCAI 2021

作者： Wu, Shangzhi Zhang, Fan Wang, xuwen Xia, ning Northwest Normal University College of Computer Science and Engineering Lanzhou China

ISBN: (纸本)9781450389501

Attribute reduction is one of the core contents in rough set knowledge discovery, how to find the minimal attribute reduction, proposes an attribute reduction method using adaptive Genetic Algorithm(GA) and Particle Swarm Optimization(PSO). On the basis of the condition attributes for decision attribute support degree, the method reset the fitness function, can dynamically adjust the parameters of the function, thus to ensure the obtained results for minimal reduction, combined with the genetic algorithm of adaptive crossover and mutation operation, to ensure that the particles in the feasible solution can be fully retain and use, the method to strengthen the local search ability at the same time, but also keep the global search ability. Experiments results show that the method has obvious advantages in solving the minimum attribute reduction. © 2021 ACM.

关键词： Genetic algorithms

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Synthesis and bioactivity of 1,3-thiazolidine-2-thione derivatives against type III secretion system of Xanthomonas oryzae

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BIOORGANIC & MEDICINAL CHEMISTRY 2019年第15期27卷 3364-3371页

作者： Tao, Hui Tian, Hao Jiang, Shan Xiang, xuwen Ahmed, Wasim Tang, Riyuan Cui, Zi-ning South China Agr Univ Integrat Microbiol Res Ctr State Key Lab Conservat & Utilizat Subtrop Agrobi Guangdong Prov Key Lab Microbial Signals & Dis Co Guangzhou 510642 Guangdong Peoples R China South China Agr Univ Dept Appl Chem Coll Mat & Energy Guangzhou 510642 Guangdong Peoples R China

Targeting virulence factors of bacterial without affecting their growth and survival, has been an initiative strategy for the development of novel anti-microbial agents. The type III secretion system (T3SS), one of essential and highly conserved virulence factors in most Gram-negative pathogenic bacteria, has been regarded as an effective target that developed new anti-microbial drugs. Xanthomonas oryzae pv. oryzae (Xoo) is one of the most important bacterial pathogens on rice, which causes leaf blight disease. To discover potential anti-virulence agents against the pathogens, a new series of 1,3-thiazolidine-2-thione derivatives containing 5-phenyl-2-furan were designed and synthesized. Their structures were characterized by H-1 NMR, C-13 NMR, MS, and elemental analysis. All the title compounds inhibited the promoter activity of a harpin gene hpa1, significantly, that were further checked for the impact on bacterial growth. The results indicated that treatment of Xoo with the title compound III-7 did not affect bacterial growth or survival. Moreover, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis showed that the expression of the Xoo T3SS was suppressed by treatment with the inhibitor. The mRNA levels of representative genes in the hrp (hypersensitive response and pathogenicity) cluster, as well as the regulatory genes hrpG and hrpX, were reduced. Finally, the in vivo test demonstrated that the compounds could reduce the disease symptoms of Xoo on the rice cultivar (Oryza sativa) IR24.

关键词： Xanthomonas oryzae pv. oryzae (Xoo) Type III secretion system (T3SS) Small molecule inhibitors Virulence factors 1,3-Thiazolidine-2-thione

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Effect of grinding depth on the rotating bending fatigue properties of 40Cr steel

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FATIGUE & FRACTURE OF ENGINEERING MATERIALS & STRUCTURES 2022年第4期45卷 1292-1295页

作者： Zhou, Honggen Chen, Jianzhi Bai, Xiaoxiang Li, Guochao Guan, Xiaoyan Du, Qin Li, ning Jing, xuwen Jiangsu Univ Sci & Technol Sch Mech Engn Zhenjiang 212003 Jiangsu Peoples R China Beijing North Vehicle Grp Co Ltd Beijing Peoples R China

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Synthesis and biological evaluation of 1,3,4-thiadiazole derivatives as type III secretion system inhibitors against Xanthomonas oryzae

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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 2019年 160卷 87-94页

作者： Tao, Hui Tian, Hao Jiang, Shan Xiang, xuwen Lin, Yinuo Ahmed, Wasim Tang, Riyuan Cui, Zi-ning South China Agr Univ Guangdong Prov Key Lab Microbial Signals & Dis Co Integrat Microbiol Res Ctr State Key Lab Conservat & Utilizat Subtrop Agrobi Guangzhou 510642 Guangdong Peoples R China South China Agr Univ Coll Mat & Energy Dept Appl Chem Guangzhou 510642 Guangdong Peoples R China

Xanthomonas oryzae pv. oryzae (Xoo) infection directly leads to a severe disease known as leaf blight, which is a major cause of yield loss of rice. Use of traditional bactericides has resulted in severe resistance in pathogenic bacteria. A new approach screening compounds that target the virulence factors rather than killing bacterial pathogens is imperative. In gram-negative bacteria, the type III secretion system (T3SS) is a conserved and significant virulence factor considered as a target for drug development. Therefore, we designed and synthesized a new series of 5-phenyl-2-furan carboxylic acid derivatives stitched with 2-mercapto-1,3,4-thiadiazole. Bioassays revealed that the title candidates attenuated the hypersensitive response through suppressing the promoter activity of a harpin gene hpa1 without affecting bacterial growth. Quantitative real time polymerase chain reaction (qRT-PCR) analysis demonstrated reduced the expression of several genes associated with T3SS, when title compounds were applied. Additionally, hrp gene cluster members, including hrpG and hrpX, had reduced mRNA levels. In vivo greenhouse tests showed that candidate compounds could alleviate the effects of Xoo infection in rice (Oryza sativa) and possess better protective activity against rice bacterial leaf blight than bismerthiazol and thiodiazole copper. All tested compounds were safe to rice. This work suggests there are new safe options for Xoo control in rice from these 1,3,4-thiadiazole derivatives.

关键词： 2-mercapto-1,3,4-thiadiazole Inhibitors Type III secretion system (T3SS) Anti-virulence factors Xanthomonas oryzae pv. oryzae (Xoo)

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Chemical compositions of mussels and clams from the Tangyin and Yonaguni Knoll IV hydrothermal fields in the southwestern Okinawa Trough

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ORE GEOLOGY REVIEWS 2017年 87卷 172-191页

作者： Zeng, Zhigang Chen, Shuai Ma, Yao Yin, Xuebo Wang, Xiaoyuan Zhang, Suping Zhang, Junlong Wu, xuwen Li, Yang Dong, Dong Xiao, ning Chinese Acad Sci Inst Oceanol Key Lab Marine Geol & Environm Seafloor Hydrothermal Act Lab 7 Nanhai Rd Qingdao 266071 Peoples R China Qingdao Natl Lab Marine Sci & Technol Lab Marine Mineral Resources Qingdao 266071 Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China Qingdao Collaborat Innovat Ctr Marine Sci & Techn Qingdao 266061 Peoples R China Chinese Acad Sci Inst Oceanol Marine Biol Museum Qingdao 266071 Peoples R China

Studies of the chemical characteristics of mussels and clams in seafloor hydrothermal fields are important for understanding mass fluxes and elemental partitioning from hydrothermal vents into the biosphere, metal bioaccumulation of seafloor hydrothermal ecosystems, and the sources and sinks of biogeochemical and fluid cycles. We are the first to measure the mineral, major, trace and rare earth element, and carbon and oxygen isotope compositions of mussels (Bathymodiolus platifrons) and clams (Conchocele bisecta) from the Tangyin and Yonaguni Knoll IV hydrothermal fields in the southwestern Okinawa Trough. Mineralogical analysis shows that the carbonate shells of the mussel and clam samples are mainly composed of calcite and aragonite. Metal elements exhibit linear correlations in the shells (e.g., V and U) and tissues (e.g., Li and Rb) of the mussels and clams, suggesting that not all positive correlations of elements in tissues are inherited by the shells. V/As, Ca/Sr, and Fe/Cr ratios in the mussels and clams are close to those in the seawater, indicating that element ratios of seawater might be inherited by the mussels and clams. In addition, the Fe/Cr ratio of the shells of both mussels and clams can be used to trace the local seawater composition. The total LREE concentrations of mussel and clam tissue samples are higher than those of the mussel and clam shell samples, are similar to the hydrothermal fluids, exhibit LREE enrichment (La-CN/Nd-CN ratios = 1.86-32.1), and no or only slightly negative Eu anomalies, indicating that benthic animals are a sink of LREEs from hydrothermal fluids, and that the Eu/Eu* ratios of fluids change when fluids are incorporated into the tissues of the mussels and clams. In addition, the delta C-13 values of mussel shell samples are heavier than those of the clam shell samples in the hydrothermal field, indicating that more than one carbon source may be involved in defining the delta C-13 compositions of the shells. The majority

关键词： Mussel Clam C hemical composition Tangyin and Yonaguni Knoll IV hydrothermal fields

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超载预压技术加固高速公路软基的试验研究

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岩石力学与工程学报 2007年第A01期26卷 3277-3282页

作者：鲁绪文娄炎何宁关秉洪南京水利科学研究院江苏南京210029

为了解超载预压技术对深厚软土的加固效果,结合杭州湾跨海大桥南岸接线高速公路试验段工程,对超载预压技术处理的试验断面进行长达2 a多的现场试验测试。实测结果表明,处理后的路基工后沉降小于15 cm,满足设计要求小于30 cm的标准;深层... 详细信息

为了解超载预压技术对深厚软土的加固效果,结合杭州湾跨海大桥南岸接线高速公路试验段工程,对超载预压技术处理的试验断面进行长达2 a多的现场试验测试。实测结果表明,处理后的路基工后沉降小于15 cm,满足设计要求小于30 cm的标准;深层压缩主要是在排水板范围内的土体中完成的;侧向位移主要发生在16 m以上部位,且7 m以上土体是向加固区内移动的,7 m以下则向加固区外移动;加固后期,加固区表面的断面沉降呈明显的锅底形;孔隙水压力变化在较浅的土层受影响明显,反应灵敏,而深层则反应较慢,孔隙水压力变化不剧烈;原位十字板测试以及室内土性试验结果表明,加固后的土性物理指标有明显的改善,土的工程力学性质有显著增强,说明超载预压排水固结法加固软土地基达到预期的目的,在类似路段中应用是可行的。

关键词：基础工程超载预压软基加固试验研究处理效果

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Inhibition of Gene Expression of Trehalase Enhances Drought Resistance in Transgenic Tobacco

Inhibition of Gene Expression of Trehalase Enhances Drought ...

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International Conference on Bioinformatics and Biomedical Engineering (ICBBE)

作者： Bei Guo Yanzhai Song xuwen Guo Wenping Wang ning Ding Dept. of Biotechnol. Beijing Univ. of Agric. Beijing China

The representative individuals, CK (non-transgenic), 121 (with pBI121 vector), 1285 (with pGSA1285 vector), T4 (with gene of trehalase, TRE), S1 (with gene of trehalose -6 - phosphate synthase, TPS), P3 (with gene of trehalose -6 - phosphate phosphatase, TPP), a3 (with anti-TRE gene), i2 (with RNAi-TRE gene), were selected for identification of drought resistance, and the Malondialdehyde (MDA), relative conductivity have been measured after water-stress during this experiment. The results showed that the transgenic tobaccos with anti-TRE and RNAi-TRE grew better than other individuals under water-stress conditions; conductivity of i2 was lower than other plants. Meantime, i2 has performance of some special features. These indicate that through application the antisense RNA and RNA interference technology to trehalase gene, it is possible to make the receptor trehalose metabolism changes. The inhibition of gene expression of trehalase can led to the accumulation of trehalose content and the enhancement of drought resistance in the transgenic tobaccos.

关键词： Gene expression Biochemistry Immune system RNA Conductivity measurement Interference Biological materials Testing Temperature Biotechnology

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基于随机森林模型的太湖水生植被遥感信息提取

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湖泊科学 2016年第3期28卷 635-644页

作者：侍昊李旭文牛志春李继影李杨李宁江苏省环境监测中心南京210036 苏州市环境监测中心站苏州215004 南京师范大学虚拟地理环境教育部重点实验室南京210023 南京大学生命科学学院南京210046

水生植被作为太湖湿地的重要组分,其数量和范围变化影响着湖泊生态系统的平衡,故利用遥感技术对水生植被的空间分布开展研究有助于太湖湿地生态系统的保护.以Landsat 8多光谱遥感影像为主要数据源,利用光谱指数和图像变换方法构建多个... 详细信息

水生植被作为太湖湿地的重要组分,其数量和范围变化影响着湖泊生态系统的平衡,故利用遥感技术对水生植被的空间分布开展研究有助于太湖湿地生态系统的保护.以Landsat 8多光谱遥感影像为主要数据源,利用光谱指数和图像变换方法构建多个特征变量,结合随机森林(RF)模型,提取太湖水生植被的空间分布.结果表明:(1)通过对比分析训练样本特征值的平均值、标准差和变异系数,NDVI、NDWIF、SR等指数更易于区分开敞水域和沉水植被、浮叶植被和挺水植被;(2)当设置1000棵分类树和4个分割节点的随机变量时,RF分类模型的袋外误分率小于6%,误分主要受SR、MNDWI和NDVI等特征变量影响;(3)通过验证分析,基于RF模型获得的2014年7月太湖水生植被覆盖面积约为306.0km2,分类精度为88.56%(Kappa系数为0.88),主要分布在湖体的东部和南部,以沉水和浮叶植被为主,两者占水生植被覆盖总面积的84.9%.

关键词：水生植被随机森林模型特征变量 Landsat 8 太湖

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TXNIP inhibition in the treatment of type 2 diabetes mellitus: design, synthesis, and biological evaluation of quinazoline derivatives

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JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY 2023年第1期38卷 Article: 2166937页

作者： Li, Aiyun Guan, Li Su, Wanzhen Zhao, ning Song, xuwen Wang, Jin Tang, Xiaoxiao Li, Weize Jiao, Xiangying Shanxi Med Univ Key Lab Cellular Physiol Minist Educ Taiyuan 030001 Peoples R China Shanxi Med Univ Dept Physiol Taiyuan 030001 Peoples R China Xian Med Univ Coll Pharm Xian 710021 Peoples R China

Thioredoxin interacting protein (TXNIP) is a potential drug target for type 2 diabetes mellitus (T2DM) treatment. A series of quinazoline derivatives were designed, synthesised, and evaluated to inhibit TXNIP expression and protect from palmitate (PA)-induced beta cell injury. In vitro cell viability assay showed that compounds D-2 and C-1 could effectively protect beta cell from PA-induced apoptosis, and subsequent results showed that these two compounds decreased TXNIP expression by accelerating its protein degradation. Mechanistically, compounds D-2 and C-1 reduced intracellular reactive oxygen species (ROS) production and modulated TXNIP-NLRP3 inflammasome signalling, and thus alleviating oxidative stress injury and inflammatory response under PA insult. Besides, these two compounds were predicted to possess better drug-likeness properties using SwissADME. The present study showed that compounds D-2 and C-1, especially compound D-2, were potent pancreatic beta cell protective agents to inhibit TXNIP expression and might serve as promising lead candidates for the treatment of T2DM.

关键词： Quinazoline derivatives TXNIP inhibition oxidative stress and inflammation pancreatic beta cell apoptosis T2DM

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