There are limited treatment options for cartilage defects in clinical practice because of the lack of suitable biomaterials. Here, we evaluated the effects of collagen type II sponge on the articular cartilage repairi...
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There are limited treatment options for cartilage defects in clinical practice because of the lack of suitable biomaterials. Here, we evaluated the effects of collagen type II sponge on the articular cartilage repairing process using a cartilage injury of a rabbit knee joint model. We showed that the home-made collagen type II sponges appeared to have a suitable pore size of 93.26 +/- 38.4 mu m for chondrocyte growth. MRI with H&E staining results demonstrated that the effusion absorption in the collagen type II sponge treated group was quicker than that of the control group. Moreover, sporadic cartilage signals first appeared at 6 weeks in the collagen type II sponge treated group. Safranin O staining and immunohistochemical analysis confirmed that the newly formed cartilage expresses glycosaminoglycan and type II collagen matrix. Using Sirius red polarized light staining, we showed that the newly formed cartilage-like areas from the collagen type II treated group are significantly greater than those of the control group. Taken together, our data demonstrated that the home-made collagen type II sponge is able to promote cartilage repair in the cartilage injury of a rabbit knee joint model. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 96B: 267-275, 2011.
The representative individuals, CK (non-transgenic), 121 (with pBI121 vector), 1285 (with pGSA1285 vector), T4 (with gene of trehalase, TRE), S1 (with gene of trehalose -6 -phosphate synthase, TPS), P3 (with gene of t...
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ISBN:
(纸本)9781424447138
The representative individuals, CK (non-transgenic), 121 (with pBI121 vector), 1285 (with pGSA1285 vector), T4 (with gene of trehalase, TRE), S1 (with gene of trehalose -6 -phosphate synthase, TPS), P3 (with gene of trehalose -6 -phosphate phosphatase, TPP), a3 (with anti-TRE gene), i2 (with RNAi-TRE gene), were selected for identification of drought resistance, and the Malondialdehyde (MDA), relative conductivity have been measured after water-stress during this experiment. The results showed that the transgenic tobaccos with anti-TRE and RNAiTRE grew better than other individuals under water-stress conditions;conductivity of i2 was lower than other plants. Meantime, i2 has performance of some special features. These indicate that through application the antisense RNA and RNA interference technology to trehalase gene, it is possible to make the receptor trehalose metabolism changes. The inhibition of gene expression of trehalase can led to the accumulation of trehalose content and the enhancement of drought resistance in the transgenic tobaccos.
The representative individuals, CK (non-transgenic), 121 (with pBI121 vector), 1285 (with pGSA1285 vector), T4 (with gene of trehalase, TRE), S1 (with gene of trehalose -6 - phosphate synthase, TPS), P3 (with gene of ...
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The representative individuals, CK (non-transgenic), 121 (with pBI121 vector), 1285 (with pGSA1285 vector), T4 (with gene of trehalase, TRE), S1 (with gene of trehalose -6 - phosphate synthase, TPS), P3 (with gene of trehalose -6 - phosphate phosphatase, TPP), a3 (with anti-TRE gene), i2 (with RNAi-TRE gene), were selected for identification of drought resistance, and the Malondialdehyde (MDA), relative conductivity have been measured after water-stress during this experiment. The results showed that the transgenic tobaccos with anti-TRE and RNAi-TRE grew better than other individuals under water-stress conditions; conductivity of i2 was lower than other plants. Meantime, i2 has performance of some special features. These indicate that through application the antisense RNA and RNA interference technology to trehalase gene, it is possible to make the receptor trehalose metabolism changes. The inhibition of gene expression of trehalase can led to the accumulation of trehalose content and the enhancement of drought resistance in the transgenic tobaccos.
Objective There are limited treatment options for cartilage defects in clinical practice due to the lack of suitable ***, we evaluated the effects of type Ⅱ collagen sponge on the articular cartilage repairing proces...
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Objective There are limited treatment options for cartilage defects in clinical practice due to the lack of suitable ***, we evaluated the effects of type Ⅱ collagen sponge on the articular cartilage repairing process using a cartilage injury of a rabbit knee joint *** showed that the type Ⅱ collagen sponges generated from our modified method appeared to have a suitable pore size of 93.26 ± 38.4 micron for chondrocyte *** with H&E staining results demonstrated that the effusion absorption in the type Ⅱ collagen sponge treated group was quicker than that of the control group.
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