检索结果-内蒙古大学图书馆

Instant and low-cost detection of urinary microalbumin using smartphone technology and a paper-based platform with a colorimetric ratio analysis

引用

SCIENTIFIC REPORTS 2025年第1期15卷 1-13页

作者： Larpant, Nutcha Tetsiri, Chanatnan rojanarata, theerasak Laiwattanapaisal, Wanida Chulalongkorn Univ Ctr Excellence Biosensors & Bioengn CEBB Bangkok 10330 Thailand Chulalongkorn Univ Fac Pharmaceut Sci Master Sci Program Res Enterprise Bangkok 10330 Thailand Silpakorn Univ Fac Pharm Dept Ind Pharm Nakhon Pathom 73000 Thailand Chulalongkorn Univ Fac Allied Hlth Sci Dept Clin Chem Bangkok 10330 Thailand

Early detection of microalbuminuria is essential for preventing the progression of nephropathy and improving patient management. The present research proposes a simple, low-cost dye-binding method based on a standard addition principle for determining urinary albumin at clinically significant levels. The assay principle of the proposed paper-based analytical device is based on utilizing a specific and sensitive dye, bis(3 ',3 ''-diiodo-4 ',4 ''-dihydroxy-5 ',5 ''-dinitrophenyl) -3,4,5,6-tetrabromosulfonphthalein (DIDNTB), which binds to urinary albumin under extreme low pH conditions. The strategy was based on testing with three different albumin concentrations in the urine samples to obtain different color intensity ratios denoted as R1, R2, and R3, and further used to calculate a semi-quantitative amount of albumin. Under the optimized conditions, the linear range from 10 to 200 mg/L albumin and the detection limit 8.2 mg/L (R2 = 0.99) was obtained. Interference studies revealed that glucose, ascorbic acid, uric acid, L-histidine, and globulin were in the acceptable recovery range (89-109%). Reproducibility assessments showed that the coefficients of variation (CV) for this method ranged between 3.7% and 7.7%. Additionally, receiver operating characteristic (ROC) plots were generated to establish the cut-off value for instant semi-quantitative interpretation of the results. Results from real sample analysis revealed that the proposed method is comparable to a widely used quantitative immunoturbidity method (r = 0.94, n = 50).

关键词： Microalbuminuria Colorimetry Smartphone Paper-based analytical device RGB detector

来源：评论

学校读者我要写书评

暂无评论

Folate receptor-targeted thiol-maleimide clicked chitosan/carboxymethyl cellulose nanoparticles for cisplatin delivery in oral carcinoma

引用

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2025年 290卷 138976页

作者： Patrojanasophon, Prasopchai Singpanna, Kanokwan rojanarata, theerasak Opanasopit, Praneet Ngawhirunpat, Tanasait Pengnam, Supusson Pornpitchanarong, Chaiyakarn Silpakorn Univ Fac Pharm Pharmaceut Dev Green Innovat Grp PDGIG Nakhon Pathom 73000 Thailand Silpakorn Univ Fac Pharm Res & Innovat Ctr Adv Therapy Med Prod Nakhon Pathom 73000 Thailand

This study aimed to develop cisplatin (CDDP)-loaded folic acid (FA)-decorated nanoparticles (NPs) as targeted drug carrier towards overexpressed folate receptors on the oral carcinoma cell line (KB cells). The FA-conjugated thiolated succinyl chitosan (FA-SH-SCS) and maleimide-grafted-carboxymethyl cellulose (CMC-MAL) were synthesized and acquired in the preparation of NPs via thiol-maleimide click reaction. The physicochemical characteristics, drug loading, and drug release of the FA-decorated NPs (FA-NPs) were examined. Also, the in vitro biocompatibility, cellular uptake, and cell death mechanism were investigated. Relatively spherical NPs with negative charge were obtained with a size of approximately 200 nm. The formation of FA-NPs through click reaction was confirmed by the pH change and Ellman's assay. The release of CDDP from the FA-NPs was influenced by the acidic tumor environment. The FA-NPs were non-toxic to the normal cells. Furthermore, FANPs improved the cellular uptake of CDDP in oral carcinoma cells through specific recognition of folate receptors by FA-NPs. The delivery of CDDP by FA-NPs to the KB cell induced the apoptotic cell death pathway. Therefore, FA-NPs presented the potential to be effective nanocarriers for CDDP delivery in the treatment of oral cancer via active targeting approach.

关键词： Cisplatin Folate receptor Polymeric nanoparticle Thiol-maleimide click chemistry Oral carcinoma cell

来源：评论

学校读者我要写书评

暂无评论

Development of a green and rapid ethanol-based HPLC assay for aspirin tablets and feasibility evaluation of domestically produced bioethanol in Thailand as a sustainable mobile phase

引用

GREEN PROCESSING AND SYNTHESIS 2025年第1期14卷

作者： Thanayutsiri, Thana Mantadilok, Siwa Sapsin, Jiradet Tungwattanaviboon, Thada Wongwatanasin, Jetsada Opanasopit, Praneet Ngawhirunpat, Tanasait rojanarata, theerasak Silpakorn Univ Fac Pharm Sanam Chandra Palace Campus Nakhon Pathom 73000 Thailand Silpakorn Univ Fac Pharm Pharmaceut Dev Green Innovat Grp PDGIG Nakhon Pathom 73000 Thailand Thai Ethanol Mfg Assoc Bangkok 10400 Thailand

Despite the growing trend of using ethanol as a greener alternative to hazardous solvents like methanol and acetonitrile in high-performance liquid chromatography (HPLC) analysis, no ethanol-based assay has been developed for aspirin tablets, nor has the potential of domestically produced bioethanol as a mobile phase been explored - until now. This study introduces a novel ethanol-based HPLC assay for aspirin and assesses the feasibility of using locally produced bioethanol. The optimized method, featuring a 40% (v/v) ethanol-water mobile phase adjusted to pH 3.6 with glacial acetic acid, with a 15-cm C18 column at 40 degrees C and UV detection at 237 nm, successfully separated aspirin and its degradation product, salicylic acid, in 5 min. It demonstrated excellent linearity (0.1-0.6 mgmL(-1), r(2) = 0.9997), sensitivity (LOD = 0.01 mgmL(-1), LOQ = 0.03 mgmL(-1)), accuracy, and precision, with no interference from excipients. The method's performance on commercial aspirin tablets aligned with official pharmacopoeial methods. In a case study using fuel-grade bioethanol (>99.5% purity) derived from sugarcane molasses or cassava from local manufacturers in Thailand, chromatographic and analytical results matched those obtained with imported ethanol, validating its effectiveness. This innovative approach not only reduces costs and dependence on imported solvents but also supports local bio-circular-green economies and promotes greener, more sustainable analytical practices.

关键词： ethanol HPLC aspirin domestic sustainability

来源：评论

学校读者我要写书评

暂无评论

How Online Whiteboard Promotes Students' Collaborative Skills in Laboratory Learning 2020

How Online Whiteboard Promotes Students' Collaborative Skill...

引用

8th International Conference on Information and Education Technology (ICIET)

作者： rojanarata, theerasak Silpakorn Univ Fac Pharm Dept Pharmaceut Chem Muang Nakhon Pathom Thailand

ISBN: (纸本)9781450377058

This work reports on the use of an online whiteboard in producing a collaborative and interactive report in laboratory learning at the Faculty of Pharmacy, Silpakorn University, Thailand. In this strategy, 166 students worked in groups of 8-9 to create experimental flow charts, to record data and to discuss results with friends on the board. Along with the students' activities, the instructors accessed the boards to monitor student progress and to provide feedback. From the questionnaires, most students reported that they were satisfied with using the tool and agreed that the learning activities were effective. While most of them had never used an online whiteboard before, they reported that the tool was not difficult to use and had useful and attractive features. Furthermore, they gained digital skills and teamwork skills from learning with the whiteboard. Therefore, online whiteboard can be used a tool to engage students in collaborative learning, active learning and formative assessment.

关键词： Online Whiteboard Laboratory Active Learning

来源：评论

学校读者我要写书评

暂无评论

Ninhydrin reaction on thiol-reactive solid and its potential for the quantitation of D-penicillamine

引用

TALANTA 2010年第2期82卷 444-449页

作者： rojanarata, theerasak Opanasopit, Praneet Ngawhirunpat, Tanasait Saehuan, Choedchai Silpakorn Univ Fac Pharm Dept Pharmaceut Chem Nakhon Pathom 73000 Thailand Naresuan Univ Fac Allied Hlth Sci Dept Med Technol Phitsanulok 65000 Thailand

While aminothiols produce weak purple-colored reactions with ninhydrin, we demonstrate for the first time that this color could be intensely developed. Using a D-penicillamine paradigm, adsorption of this compound via a disulfide bond onto thiol-reactive solid prior to ninhydrin reaction allowed spectrophotometrical monitoring of the supernatant at 570 nm. Compared with off-solid method, this approach expanded the linear concentration range to 50-600 mu g mL(-1) and enhanced the sensitivity so that D-penicillamine with the concentrations of less than 100 mu g mL(-1) could be accurately quantitated by using a second-order polynomial calibration curve. Additionally, this assay was unaffected by disulfide adduct interference, highlighting its potential for the analysis of D-penicillamine as well as other aminothiols. (C) 2010 Elsevier B.V. All rights reserved.

关键词： Ninhydrin Thiol-reactive solid Penicillamine Aminothiol

来源：评论

学校读者我要写书评

暂无评论

Fast, facile and ethidium bromide-free assay based on the use of adsorption indicator for the estimation of polyethylenimine to nucleic acid ratio of complete polyplex assembly for gene delivery

引用

TALANTA 2013年 115卷 241-245页

作者： Plianwong, Samarwadee Opanasopit, Praneet Ngawhirunpat, Tanasait rojanarata, theerasak Silpakorn Univ Pharmaceut Dev Green Innovat Grp PDGIG Fac Pharm Nakhon Pathom 73000 Thailand

A new method was developed for the estimation of polyethylenimine (PEI) to nucleic acid ratio at which the polyplex was completely formed. The assay relied on the attraction of dichlorofluoresceinate dye to adsorb on self-assembling particles as counterions, as induced by the surface charge of the polyplex which became positive once PEI associated equivalently with nucleic acid. This phenomenon resulted in the appearance of pink colored pellets of the polyplex after centrifugation. By the other means, sodium hydroxide solution might be added to free the adsorbed dye into the solution, producing conspicuous green fluorescence under UV light (366 nm). The assay was well applied to the polyplex formulations of PEI and plasmid DNA or siRNA with satisfactory detectability and gave the results in agreement with those from gel retardation method and zeta potential analysis. Importantly, the proposed method required no sophisticated instruments, time-consuming gel electrophoresis, carcinogenic ethidium bromide as well as costly dyes and the analysis could be accomplished within less than 10 min. Hence, it was a fast, facile, cost-effective and safe-for-operator alternative method, suited for the investigation of the optimal PEI to nucleic acid ratio for gene delivery. (C) 2013 Elsevier B.V. All rights reserved.

关键词： Polyethylenimine Nucleic acid Ratio Polyplex Dichlorofluorescein Surface charge

来源：评论

学校读者我要写书评

暂无评论

Enhancement of Skin Permeation and Skin Immunization of Ovalbumin Antigen via Microneedles

引用

AAPS PHARMSCITECH 2017年第7期18卷 2418-2426页

作者： Pamornpathomkul, Boonnada rojanarata, theerasak Opanasopit, Praneet Ngawhirunpat, Tanasait Silpakorn Univ Fac Pharm PDGIG Nakhon Pathom 73000 Thailand

The purpose of this study was to evaluate the use of different types of microneedles and doses of ovalbumin antigen for in vitro skin permeation and in vivo immunization. In vitro skin permeation experiments and confocal laser scanning microscopy revealed that hollow microneedles had a superior enhancing effect on skin permeation compared with a solid microneedle patch and untreated skin by efficiently delivering ovalbumin-fluorescein conjugate into the deep skin layers. The flux and cumulative amount of ovalbumin-fluorescein conjugate at 8 h after administering with various conditions could be ranked as follows: hollow MN;high dose > medium dose > low dose > MN patch;high dose > medium dose > low dose > untreated skin;high dose > medium dose > low dose > without ovalbumin-fluorescein conjugate. As the dose of ovalbumin-fluorescein conjugate was increased to 500 mu g, the antigen accumulated in the skin to a greater extent, as evidenced by the increasing green fluorescence intensity. When the hollow microneedle was used for the delivery of ovalbumin into the skin of mice, it was capable of inducing a stronger immunoglobulin G immune response than conventional subcutaneous injection at the same antigen dose. Immunoglobulin G levels in the hollow MN group were 5.7, 11.6, and 13.3 times higher than those of the subcutaneous injection group for low, medium, and high doses, respectively. Furthermore, the mice immunized using the hollow microneedle showed no signs of skin infection or pinpoint bleeding. The results suggest that the hollow MN is an efficient device for delivering the optimal dose of antigen via the skin for successful immunization.

关键词： hollow microneedle ovalbumin skin immunization solid microneedle

来源：评论

学校读者我要写书评

暂无评论

Preactivated-thiolated polyacrylic acid/1-vinyl pyrrolidone nanoparticles as nicotine carriers for smoking cessation

引用

RSC ADVANCES 2020年第55期10卷 33517-33525页

作者： Pornpitchanarong, Chaiyakarn rojanarata, theerasak Opanasopit, Praneet Ngawhirunpat, Tanasait Patrojanasophon, Prasopchai Silpakorn Univ Fac Pharm Pharmaceut Dev Green Innovat Grp PDGIG Muang 73000 Nakhon Pathom Thailand

This study aimed to develop nicotine-loaded mucoadhesive preactivated-thiolated polymeric nanoparticles (PNPs) for smoking cessation. 2-Mercaptonicotinic acid (2MNA) was coupled as dithionicotinic acid dimer and used in the preactivation of thiolated polyacrylic acid/vinyl pyrrolidone PNPs (thiolated AA/VP PNPs). Preactivated-thiolated AA/VP PNPs were synthesized through surfactant-free emulsion polymerization and coupling reactions. The structural attributes of the preactivated-thiolated AA/VP PNPs were characterized using Fourier-transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy. The particle size and zeta potential were evaluated by dynamic light scattering evaluation. The morphology of the preactivated-thiolated AA/VP PNPs was examined using scanning electron microscopy. In addition, the mucoadhesive properties, drug loading and release, and biocompatibility of the preactivated-thiolated AA/VP PNPs were assessed. The spherical preactivated-thiolated AA/VP PNPs were successfully synthesized with a particle size of 410.3 +/- 7.4 nm and a negative surface charge. The preactivated-thiolated AA/VP PNPs exhibited superior mucoadhesive properties compared with the thiolated AA/VP PNPs. Drug loading by PNP to a nicotine ratio of 1 : 1 provided desirable loading capacity and % loading efficiency of 285.7 +/- 36.7 mu g mg(-1)and 57.1 +/- 7.4%, respectively. More than 50% of the nicotine contained in the PNPs was rapidly released in the first hour, followed by a sustained release for up to 12 h. Moreover, the synthesized PNPs were non-toxic to human gingival cells. Therefore, the preactivated-thiolated AA/VP PNPs may be a candidate carrier of nicotine for smoking cessation.

关键词： Fourier transform infrared spectroscopy

来源：评论

学校读者我要写书评

暂无评论

One-enzyme catalyzed simultaneous plant cell disruption and conversion of released glycoside to aglycone combined with in situ product separation as green one-pot production of genipin from gardenia fruit

引用

ENZYME AND MICROBIAL TECHNOLOGY 2013年第2期53卷 92-96页

作者： Winotapun, Weerapath Opanasopit, Praneet Ngawhirunpat, Tanasait rojanarata, theerasak Silpakorn Univ Pharmaceut Dev Green Innovat Grp Fac Pharm Nakhon Pathom 73000 Thailand

A direct one-pot production of genipin, an iridoid aglycone, from crude gardenia fruit was developed. The method relied on the use of single cellulase to disrupt plant cells and to cleave off sugar molecules simultaneously, thereby enhancing the release of intracellular iridoids and converting geniposide to genipin. During the biocatalysis, eco-friendly ethyl acetate was used to extract the product, providing the partial purification and the minimization of genipin degradation. By using 10 mg/mL cellulase and 24 h-incubation at 50 degrees C, pH 4, combined with in situ extraction, genipin with good purity was yielded at 58.83 mg/g, which increased 12.38 and 1.72 times compared with those obtained from the procedures without either the aid, of enzyme or in situ extraction, respectively. Therefore, this integrated approach is promising for the production of genipin and should be potentially applied to the preparation of other plant aglycones. (C) 2013 Elsevier Inc. All rights reserved.

关键词： Genipin Enzyme assisted extraction In situ product separation Cellulase

来源：评论

学校读者我要写书评

暂无评论

Electrospun chitosan/polyvinyl alcohol nanofibre mats for wound healing

引用

INTERNATIONAL WOUND JOURNAL 2014年第2期11卷 215-222页

作者： Charernsriwilaiwat, Natthan rojanarata, theerasak Ngawhirunpat, Tanasait Opanasopit, Praneet Silpakorn Univ Fac Pharm Pharmaceut Dev Green Innovat Grp PDGIG Nakhon Pathom 73000 Thailand

Chitosan (CS) aqueous salt blended with polyvinyl alcohol (PVA) nanofibre mats was prepared by electrospinning. CS was dissolved with hydroxybenzotriazole (HOBt), thiamine pyrophosphate (TPP) and ethylenediaminetetraacetic acid (EDTA) in distilled water without the use of toxic or hazardous solvents. The CS aqueous salts were blended with PVA at different weight ratios, and the effect of the solution ratios was investigated. The morphologies and mechanical and swelling properties of the generated fibres were analysed. Indirect cytotoxicity studies indicated that the CS/PVA nanofibre mats were non-toxic to normal human fibroblast cells. The CS-HOBt/PVA and CS-EDTA/PVA nanofibre mats demonstrated satisfactory antibacterial activity against both gram-positive and gram-negative bacteria, and an in vivo wound healing test showed that the CS-EDTA/PVA nanofibre mats performed better than gauze in decreasing acute wound size during the first week after tissue damage. In conclusion, the biodegradable, biocompatible and antibacterial CS-EDTA/PVA nanofibre mats have potential for use as wound dressing materials.

关键词： Chitosan Electrospinning Nanofibre Polyvinyl alcohol Wound healing

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：