检索结果-内蒙古大学图书馆

Variability in competitive fitness among environmental and clinical azole-resistant Aspergillus fumigatus isolates

MBIO 2024年第4期15卷 e0026324页

作者： Chen, Shu Zhu, Guoxing lin, Huiping Guo, Jian Deng, shuwen Wu, Wenjuan Goldman, Gustavo H. Lu, ling Zhang, Yuanwei Nanjing Normal Univ Coll Life Sci Jiangsu Engn & Technol Res Ctr Microbiol Jiangsu Key Lab Microbes & Funct Genom Nanjing Peoples R China Tongji Univ Sch Med Shanghai East Hosp Dept Lab Med Shanghai Peoples R China Peoples Hosp SND Suzhou New Dist Suzhou Peoples R China Univ Sao Paulo Fac Ciencias Farmaceut Ribeirao Preto Ribeirao Preto Brazil

Azoles are the primary antifungal drugs used to treat infections caused by Aspergillus fumigatus. However, the emergence of azole resistance in A. fumigatus has become a global health concern despite the low proportion of resistant isolates in natural populations. In bacteria, antibiotic resistance incurs a fitness cost that renders strains less competitive in the absence of antibiotics. Consequently, fitness cost is a key determinant of the spread of resistant mutations. However, the cost of azole resistance and its underlying causes in A. fumigatus remain poorly understood. In this observation, we revealed that the 10 out of 15 screened azole-resistant isolates, which possessed the most common azole-targeted cyp51A mutations, particularly the presence of tandem repeats in the promoter region, exhibit fitness cost when competing with the susceptible isolates in azole-free environments. These results suggest that fitness cost may significantly influence the dynamics of azole resistance, which ultimately contributes to the low prevalence of azole-resistant A. fumigatus isolates in the environment and clinic. By constructing in situ cyp51A mutations in a parental azole-susceptible strain and reintroducing the wild-type cyp51A gene into the azole-resistant strains, we demonstrated that fitness cost is not directly dependent on cyp51A mutations but is instead associated with the evolution of variable mutations related to conidial germination or other unknown development-related processes. Importantly, our observations unexpectedly revealed that some azole-resistant isolates showed no detectable fitness cost, and some even exhibited significantly increased competitive fitness in azole-free environments, highlighting the potential risk associated with the prevalence of these isolates. IMPORTANCE Azole resistance in the human fungal pathogen Aspergillus fumigatus presents a global public health challenge. Understanding the epidemic trends and evolutionary patterns of azole

关键词： Aspergillus fumigatus azole resistance fitness cost

来源：评论

学校读者我要写书评

暂无评论

Activation of Piezo1 promotes osteogenic differentiation of aortic valve interstitial cell through YAP-dependent glutaminolysis

引用

SCIENCE ADVANCES 2023年第22期9卷 eadg0478页

作者： Zhong, Guoheng Su, shuwen Li, Juncong Zhao, Hengli Hu, Dongtu Chen, Jun Li, Shichao lin, Yingwen Wen, Liming lin, Xiangjie Xian, Gaopeng Xu, Dingli Zeng, Qingchun Southern Med Univ Nanfang Hosp Dept Cardiol State Key Lab Organ Failure Res Guangzhou 510515 Peoples R China Southern Med Univ Guangdong Prov Key Lab Shock & Microcirculat Guangzhou 510515 Peoples R China Bioland Lab Guangzhou Regenerat Med & Hlth Guangdo Guangzhou Peoples R China

Hemodynamic overload and dysregulation of cellular metabolism are involved in development of calcific aortic valve disease (CAVD). However, how mechanical stress relates to metabolic changes in CAVD remains unclear. Here, we show that Piezo1, a mechanosensitive ion channel, regulated glutaminase 1 (GLS1)-mediated gluta-minolysis to promote osteogenic differentiation of valve interstitial cells (VICs). In vivo, two models of aortic valve stenosis were constructed by ascending aortic constriction (AAC) and direct wire injury (DWI). Inhibition of Piezo1 and GLS1 in these models respectively mitigated aortic valve lesion. In vitro, Piezo1 activation induced by Yoda1 and oscillatory stress triggered osteogenic responses in VICs, which were prevented by Piezo1 inhi-bition or knockdown. Mechanistically, Piezo1 activation promoted calcium-dependent Yes-associated protein (YAP) activation. YAP modulated GLS1-mediated glutaminolysis, which enhanced osteogenic differentiation through histone acetylation of runt-related transcription factor 2 (RUNX2) promoters. Together, our work pro-vided a cross-talk between mechanotransduction and metabolism in the context of CAVD.

关键词： Chemical activation

来源：评论

学校读者我要写书评

暂无评论

Demonstration of Near-Size-Independent External Quantum Efficiency for 368 nm UV Micro-LEDs

引用

PHYSICA STATUS SOLIDI-RAPID RESEARCH LETTERS 2024年第11期18卷

作者： Wang, Guangying Xie, shuwen Li, Yuting Zhang, Wentao Vigen, Jonathan Shih, Timothy lin, Qinchen Gong, Jiarui Ma, Zhenqiang Pasayat, Shubhra S. Gupta, Chirag Univ Wisconsin Dept Elect & Comp Engn Madison WI 53706 USA Univ Wisconsin Dept Mat Sci & Engn Madison WI 53706 USA

UV-ranged micro-LEDs are being explored for numerous applications due to their high stability and power efficiency. However, previous reports have shown reduced external quantum efficiency (EQE) and increased leakage current due to the increase in surface-to-volume ratio with a decrease in the micro-LED size. Herein, the size-related performance for UV-A micro-LEDs, ranging from 8 x 8 to 100 x 100 mu m(2), is studied. These devices exhibit reduced leakage current with the implementation of atomic layer deposition-based sidewall passivation. A systematic EQE comparison is performed with minimal leakage current and a size-independent on-wafer EQE of around 5.5% is obtained. Smaller sized devices experimentally show enhanced EQE at high current density due to their improved heat dissipation capabilities. To the best of authors' knowledge, this is the highest reported on-wafer EQE demonstrated in <10 mu m dimensioned 368 nm UV LEDs.

关键词： EQE Gallium nitride Microfabrication Micro-LEDs UV LEDs

来源：评论

学校读者我要写书评

暂无评论

Excitation- & concentration-dependent color-tunable ultra-long phosphorescence of isophthalonitriles doped polymers

引用

CHEMICAL ENGINEERING JOURNAL 2023年第1期469卷

作者： Zheng, Xiaoxin Jiang, Jinke lin, Qinglian Li, Cuicui Chen, Jiashuai Wang, shuwen Han, Quanxiang Ye, Xin Liu, Yang Tao, Xutang Shandong Univ Inst Crystal Mat State Key Lab Crystal Mat Jinan 250100 Peoples R China

Room-temperature phosphorescence (RTP) from organic materials have been widely studied especially in the recent decades, however, the majority can only release a single-color phosphorescence after removal of the excitation. Achieving color-tunable RTP from pure organic materials is attractive but still challenging. Herein, we report a family of color-tunable RTP materials by doping isophthalonitriles into polymers. The doped polymers not only possess adjustable phosphorescence from blue to green by controlling the excitation wavelength and the doping concentration, but also exhibit ultralong lifetimes (1048 ms for green-phosphorescence and 872 ms for blue-phosphorescence) and afterglow times (>10 s). Multi-level encryption was demonstrated by utilizing the multicolored phosphorescence realized through alternation of the excitation wavelength and adoption of different concentration doped polymers. Considering all the involved components are commercially available with low-cost, such a color-tunable and long afterglow system should be potential toward a broad field of advanced anti-counterfeiting applications.

关键词： Room temperature phosphorescence Color-tunable Excitation-dependent Concentration-dependent Anti-counterfeiting

来源：评论

学校读者我要写书评

暂无评论

Effects of food, salinity, and ammonia-nitrogen on the physiology of juvenile seahorse (Hippocampus erectus) in two typical culture models in China

引用

AQUACULTURE 2020年第0期520卷 734965-000页

作者： Huang, Jiaxin Qin, Geng Zhang, Bo Tan, shuwen Sun, Jinhui lin, Qiang Chinese Acad Sci South China Sea Inst Oceanol CAS Key Lab Trop Marine Bioresources & Ecol Guangzhou 510275 Guangdong Peoples R China Tianjin Agr Univ Tianjin 300384 Peoples R China Chinese Acad Sci Inst South China Sea Ecol & Environm Engn Guangzhou 510275 Guangdong Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China

Food and water environments are key factors affecting juvenile survival rate in seahorse aquaculture. In order to adjust to local conditions, different seahorse (Hippocampus erectus) breeding models were developed in North and South China. Seahorses in North China were commonly fed Anemia in indoor containers with a stable water environment, while most seahorses in South China were fed copepods in outdoor concrete ponds. Firstly, we compared the effect of food type on juvenile seahorses based on a one-month feeding experiment and found no significant difference in body length. However, the insulin-like growth factor 1 (IGF-1) gene expression was significantly higher in the liver of seahorses fed copepods than in the lever of seahorses fed Anemia, suggesting that copepods promoted better growth. A high expression of stress-related genes, namely, heat shock proteins 70 (HSP70) and Mn superoxide dismutase (Mn-SOD), in the liver of the seahorses that were fed copepods suggested that copepods might cause more stress on juveniles. Lower leptin gene expression in the liver of seahorses fed Anemia than in those fed copepods proved that Anemia was better for satiating the juveniles. Furthermore, the potential effects of salinity (15 and 30 ppt) and ammonia-nitrogen (0, 5, and 15 mg/L) on juvenile seahorse physiological stress were studied through a 5-d orthogonal experiment. Both lower salinity and higher ammonia-nitrogen increased the ventilation rate significantly. Low salinity caused a rapid increase in the ventilation rate on day 1, while high ammonia-nitrogen was the main factor during the later period (days 2-5). Significant changes in the mRNA expression of genes, including Na+: K+:2Cl(-) cotransporter 1 (NKCC1) and Na, K-ATPase (NKA) in the gill, HSP70, Mn-SOD, glutamate dehydrogenase (GDH), and glutamyl synthase (GS) in the liver, indicated that seahorses are sensitive to acute salinity decline. In addition, high ammonia-nitrogen exposure significantly changed the e

关键词： Juvenile seahorse Diet Salinity Ammonia-nitrogen

来源：评论

学校读者我要写书评

暂无评论

THERAPEUTIC EFFECTS OF CHITIN FROM PLEUROTUS ERYNGII ON HIGH-FAT DIET INDUCED OBESITY IN RATS

引用

ACTA SCIENTIARUM POLONORUM-TECHNOLOGIA ALIMENTARIA 2020年第3期19卷 279-289页

作者： Huang, Jiafu Wu, Qici lin, Zhichao Liu, shuwen Su, Qiaoling Pan, Yutian Minnan Normal Univ Collaborat Innovat Ctr Mushroom Hlth Ind Zhangzhou 363000 Fujian Peoples R China Minnan Normal Univ Engn Technol Ctr Mushroom Ind Zhangzhou 363000 Fujian Peoples R China Minnan Normal Univ Sch Life Sci Zhangzhou 363000 Fujian Peoples R China Minnan Normal Univ Biotechnol Coll Zhangzhou 363000 Fujian Peoples R China

Background. Recent shifts in lifestyles and diets have caused the incidence of obesity to increase rapidly, resulting in a serious threat to modern human health. There is a growing interest the use of plant or fungi derived supplements as a safe and effective means to treat obesity. In recent times, edible-medicinal fungi have garnered attention as therapeutics owing to their biocompatibility and effectiveness. Attempts to determine the therapeutic effects of these fungi have become a prime focus in drug discovery programs. Therefore, this study aimed to determine the anti-obesity effects of P. eryngii chitin in rats with obesity induced by administration of a high fat diet. Material and methods. To investigate the therapeutic effects of chitin from Pleurotus eryngii on high fat diet-induce obesity, we treated obese rats with different concentrations of chitin from P. eryngii for 4 weeks, using Lipitor as positive control. The living condition, food intake, body weight, perirenal adipose tissue, periepididymal adipose tissue, adipose tissue coefficient, serum lipid levels, including total cholesterol (TC), total glyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C), were measured, and levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), themalonaldehyde (MDA), and superoxide dismutase (SOD) activity in liver were determined. The rats were also monitored for pathological changes in the liver and aorta. Results. These studies indicated that administration of chitin from P. eryngii could significantly decrease obese rat food utilization rates and accumulation of adipose tissue in the body, thus preventing development of increased body weight. The treatment also significantly reduced serum lipid levels, including levels of TC, TG and LDL-C. Treatment with P. eryngii-derived chitin also enhanced ALT and AST enzymatic activity, enhanced SOD enzymatic activity, and reduced the MDA content of the

关键词： Pleurotus eryngii chitin obesity therapy

来源：评论

学校读者我要写书评

暂无评论

脱支和重结晶法制备抗性糊精的结构和功能特性分析

引用

食品工业科技 2024年第5期45卷 37-43页

作者：徐美琪李思漫刘欣欧月灵罗舒雯李琳陈旭朱杰东莞理工学院生命健康与技术学院中国轻工业健康食品开发与营养调控重点实验室东莞市特色食品精准设计重点实验室食品营养健康工程与智能化加工研究中心广东东莞523808 华南农业大学食品学院广东广州510642

为探究脱支和重结晶法制备抗性糊精的结构和功能特性。以蜡质玉米和普通玉米淀粉为原料,采用脱支和重结晶法制备抗性糊精,对其形貌特征、结晶结构、热力学特性、官能团、糖苷键等变化进行表征,并探究其抗消化特性。结果表明:与原淀粉相... 详细信息

为探究脱支和重结晶法制备抗性糊精的结构和功能特性。以蜡质玉米和普通玉米淀粉为原料,采用脱支和重结晶法制备抗性糊精,对其形貌特征、结晶结构、热力学特性、官能团、糖苷键等变化进行表征,并探究其抗消化特性。结果表明:与原淀粉相比,两种抗性糊精热稳定性和溶解度显著增加(P<0.05),具有良好的色泽。两种抗性糊精形貌粗糙、呈不规则形状的小碎片聚集体。蜡质玉米抗性糊精呈典型的A型结晶结构,普通玉米抗性糊精呈典型的B型结晶结构。由核磁共振波谱和红外结果可知,抗性糊精在制备过程中糖苷键断裂重新聚合形成新的抗消化糖苷键,无新的官能团产生。两种抗性糊精的抗消化性能力增加,且普通玉米淀粉制备的抗性糊精的慢消化和抗消化性能力最强。综上所述,脱支和重结晶法能提高抗性糊精的抗消化能力,将为抗性糊精的绿色制备工艺进而实现产业化生产提供新的思路和理论指导。

关键词：抗性糊精脱支重结晶结构功能特性消化性

来源：评论

学校读者我要写书评

暂无评论

Manganese dioxide nanozyme for reactive oxygen therapy of bacterial infection and wound healing

引用

BIOMATERIALS SCIENCE 2021年第17期9卷 5965-5976页

作者： Liu, Li Wang, Cheng Li, Yuting Qiu, lin Zhou, shuwen Cui, Pengfei Jiang, Pengju Ni, Xinye Liu, Runhui Du, Xuancheng Wang, Jianhao Xia, Jiang Changzhou Univ Sch Pharm Changzhou 213164 Jiangsu Peoples R China Nanjing Med Univ Affiliated Changzhou Peoples Hosp 2 Changzhou Jiangsu Peoples R China East China Univ Sci & Technol State Key Lab Bioreactor Engn Sch Mat Sci & Engn Shanghai 200237 Peoples R China Shandong Univ Sch Phys Inst Adv Interdisciplinary Sci Jinan 250100 Peoples R China Chinese Univ Hong Kong Dept Chem Shatin Hong Kong Peoples R China

Reactive oxygen species (ROS) are the weapons of neutrophiles against bacterial pathogens, and also the central effectors in reactive oxygen therapy for skin and soft tissue infection. Nanozymes that spontaneously generate ROS under physiological conditions are new antibacterials that hold promise towards multidrug resistant pathogens. The clinical use of the nanozymes is however limited by their low biocompatibility and toxicity in vivo. Here, we develop an oleic acid (OA) nanoemulsion template method for the one-pot synthesis of OA-manganese dioxide (MnO2) nanozyme. The OA-MnO2 nanozyme showed high stability and biocompatibility under physiological conditions with marked oxidase-like activity. The ROS generated by the OA-MnO2 nanozyme effectively kill the Gram-positive Staphylococcus aureus and the Gram-negative Escherichia coli strains. Moreover, the OA-MnO2 nanozyme shows promising abilities to prevent and destruct biofilm formation by Staphylococcus aureus, and result in superior in vivo antibacterial performance as compared to vancomycin. The reactive oxygen therapy based on OA-MnO2 nanozyme cures the infected skin and promotes wound healing in mice, manifesting its potential use in skin and soft tissue infection.

关键词： Tissue

来源：评论

学校读者我要写书评

暂无评论

A general approach to simulate the atom distribution, lattice distortion, and mechanical properties of multi-principal element alloys based on site preference: Using FCC_CoNiV and CoCrNi to demonstrate and compare

引用

JOURNAL OF ALLOYS AND COMPOUNDS 2023年第Part1期935卷

作者： Chen, Rong Xie, Tianliang Wu, Bo Weng, Liangji Ali, Hamid Yang, shuwen Zhao, Yan Zhao, Panhong Zhang, Chubo Cao, Renhui Wen, Jiansen Yao, Qipeng Cai, Qi Zhang, Haifeng Sa, Baisheng Wen, Cuilian lin, Maohua Sun, Xu Su, Hang Liu, Yu Wang, Chunxu Fuzhou Univ Coll Mat Sci & Engn Key Lab Ecomat Adv Technol Multiscale Computat Mat Facil Fuzhou 350100 Peoples R China Fuzhou Univ Sch Adv Mfg Mat Design & Manufacture Simulat Facil Jinjiang 362200 Peoples R China Florida Atlantic Univ Dept Ocean & Mech Engn Boca Raton FL 33431 USA Cent Iron & Steel Res Inst Grp Beijing 100081 Peoples R China Fuzhou Univ Fuzhou 350100 Peoples R China

The multi-principal element alloys (MPEAs), which are also called high-entropy alloys (HEAs) or mediumentropy alloys (MEAs) based on the multi-principal element number, received intensive attention due to their unusual phase structures and potential application in diverse aspects. However, quantitative and graphical characterization of the atom distribution, lattice distortion, and mechanical properties are considerably insufficient, which hinders the exploration and development of the rich ore of MPEAs. In this work, a general and comprehensive approach was proposed to simulate the atom distribution, lattice distortion, and mechanical properties of MPEAs based on site preference, where FCC_CoNiV and CoCrNi MPEAs were demonstrated and compared quantitatively and graphically. For this purpose, the temperatureand composition-dependent site occupying fractions (SOFs) of MPEAs were predicted using a two-sublattice model based on the crystallographic information of the prototype of MPEAs, where the corresponding thermodynamic database concerning the temperature-dependent Gibbs free energy of formation of the involved end-member compounds was established in this work using first-principles calculations based on density-functional theory (DFT) and density-functional perturbation theory (DFPT). For FCC_CoNiV MPEA, the site occupying configuration is (V1.0000)1a(Co0.4444Ni0.4445V0.1111)3c, which is not affected by the heat treatment temperature, while for FCC_CoCrNi MPEA, the site preferences change continuously and slightly with the increase of the heat treatment temperature, for example, the site occupying configuration changes from (Co0.3371Cr0.0002Ni0.6627)1a(Co0.3320Cr0.4444Ni0.2236)3c at 673 K to (Co0.3690Cr0.0007Ni0.6303)1a (Co0.3214Cr0.4442Ni0.2344)3c at 1273 K. From the long-range ordered structures, the locally ordered structures were explored further by statistically analyzing the coordination number of the atom coordinated with the same type of atoms in a 30x30x3

关键词： Multi -principal element alloys (MPEAs) Site preference Local ordering behaviors Lattice distortion Mechanical properties Computational materials science

来源：评论

学校读者我要写书评

暂无评论

Antibacterial Photodynamic Gold Nanoparticles for Skin Infection

引用

ACS APPLIED BIO MATERIALS 2021年第4期4卷 3124-3132页

作者： Qiu, lin Wang, Cheng Lan, Min Guo, Qianqian Du, Xuancheng Zhou, shuwen Cui, Pengfei Hong, Tingting Jiang, Pengju Wang, Jianhao Xia, Jiang Changzhou Univ Sch Pharm Changzhou 213164 Jiangsu Peoples R China Shandong Univ Sch Phys Inst Adv Interdisciplinary Sci Jinan 250100 Peoples R China Chinese Univ Hong Kong Dept Chem Shatin Hong Kong Peoples R China

Damage or injury to the skin creates wounds that are vulnerable to bacterial infection, which in turn retards the process of skin regeneration and wound healing. In patients with severe burns and those with chronic diseases, such as diabetes, skin infection by multidrugresistant bacteria can be lethal. Therefore, a broad-spectrum therapy to effectively eradicate bacterial infection through a mechanism different from that of antibiotics is much sought after. We successfully synthesized antibacterial photodynamic gold nanoparticles (AP-AuNPs), which are self-assembled nanocomposites of an antibacterial photodynamic peptide and poly(ethylene glycol) (PEG)-stabilized AuNPs. The AP-AuNPs exhibited aqueous and light stability, a satisfactory generation of reactive oxygen species (ROS), and a remarkable antibacterial effect toward both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli upon light irradiation. Moreover, the synthesized nanocomposites significantly inhibited bacterial growth and biofilm formation in vitro. Photodynamic antibacterial treatment accelerated the wound-healing rate in S. aureus infections, mimicking staphylococcal skin infections. Using a combination of the bactericidal effect of a peptide, the photodynamic effect of a photosensitizer, and the multivalency clustering on AuNPs for maximal antibacterial effect under light irradiation, we synthesized AP-AuNPs as a wound-dressing nanomaterial in skin infections to promote wound healing. Our findings indicate a promising strategy in the management of bacterial infections resulting from damaged skin tissue, an aspect that has not been fully explored by our peers.

关键词： antibacterial photodynamic nanoparticles staphylococcal skin infection antibacterial peptide photosensitizer antibacterial photodynamic therapy

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：