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Inhibition of cellular inflammatory mediator production and amelioration of learning deficit in flies by deep sea Aspergillus-derived cyclopenin

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JOURNAL OF ANTIBIOTICS 2020年第9期73卷 622-629页

作者： Wang, Liyan Li, Mengjie lin, Yinzhi Du, shuwen Liu, Zhenyu Ju, Jianhua Suzuki, Hiromi Sawada, Makoto Umezawa, Kazuo Shenzhen Univ Shenzhen Key Lab Marine Bioresource & Ecoenvironm Coll Life Sci & Oceanog Shenzhen 518060 Peoples R China Aichi Med Univ Sch Med Dept Mol Target Med Nagakute Aichi 4801195 Japan Aichi Med Univ Sch Med Dept Microbiol & Immunol Nagakute Aichi 4801195 Japan Beijing JoeKai Biotech LLC Beijing 10094 Peoples R China Chinese Acad Sci South China Sea Inst Oceanol RNAM Ctr Marine Microbiol Key Lab Trop Marine Bioresources & Ecol Guangzhou 510301 Guangdong Peoples R China Nagoya Univ Environm Med Res Inst Brain Funct Nagoya Aichi 4648601 Japan

In the course of screening lipopolysaccharide (LPS)-induced nitric oxide (NO) production inhibitors, two related benzodiazepine derivatives, cyclopenol and cyclopenin, were isolated from the extract of a deep marine-derived fungal strain, Aspergillus sp. SCSIOW2. Cyclopenol and cyclopenin inhibited the LPS-induced formation of NO and secretion of IL-6 in RAW264.7 cells at nontoxic concentrations. In terms of the mechanism underlying these effects, cyclopenol and cyclopenin were found to inhibit the upstream signal of NF-kappa B activation. These compounds also inhibited the expression of IL-1 beta, IL-6, and inducible nitric oxide synthase (iNOS) in mouse microglia cells, macrophages in the brain. In relation to the cause of Alzheimer's disease, amyloid-beta-peptide is known to induce inflammation in the brain. Therefore, the present study investigated the ameliorative effects of these inhibitors on an in vivo Alzheimer's model using flies. Learning deficits were induced by the overexpression of amyloid-beta 42 in flies, and cyclopenin but not cyclopenol was found to rescue learning impairment. Therefore, novel anti-inflammatory activities of cyclopenin were identified, which may be useful as a candidate of anti-inflammatory agents for neurodegenerative diseases.

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Effect of different dopants on porous calcium silicate composite bone scaffolds by 3D gel-printing

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CERAMICS INTERNATIONAL 2020年第1期46卷 325-330页

作者： Zhang, Zhinan lin, Tao Shao, Huiping Peng, Jiang Wang, Aiyuan Zhang, Yumeng Yu, Xinding Liu, shuwen Wang, Luhui Zhao, Manjian Univ Sci & Technol Beijing Inst Adv Mat & Technol Beijing 100083 Peoples R China Chinese Peoples Liberat Army Gen Hosp Inst Orthoped Beijing 100853 Peoples R China Key Lab Musculoskeletal Trauma & War Injuries PLA Key Lab Regenerat Med Orthoped Beijing 100853 Peoples R China

Porous CaSiO3 composite scaffolds with different dopants, such as MgSiO3, MgCl2 and CaSO4, were successfully prepared by 3D gel-printing (3DGP). (m, n) is proposed to describe the filament geometry features. The results show that doping can improve the strength of porous composite scaffolds and MgCl2-doped composite scaffolds had the highest modulus of elasticity of 1241 MPa. The shrinkage rate range of the composite scaffolds was 11.44-13.16%, and their porosity was all about 60%. When porous composite scaffolds were soaked in SBF for 28 days at 37 degrees C, the degradation rate was 2.7% (pure), 0.3% (MgSiO3), 0.2% (MgCl2), 5.27% (CaSO4), respectively. It explains that MgSiO3 and MgCl2 inhibited the in vitro degradation of CaSiO3, while CaSO4 promoted. It is obviously that doped MgCl2 can improve the mechanical properties of porous scaffolds, and doped CaSO4 can improve the degradation of scaffolds, which play an important role in bone repair engineering.

关键词： 3DGP CaSiO3 Porous Doped

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Phylogenetic characteristics of HIV among female cross-border travelers in Yunnan province between 2003 and 2012

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JOURNAL OF MEDICAL VIROLOGY 2021年第8期93卷 5173-5176页

作者： Ting, Yang Liang, shuwen Yang, Shuting lin, Yihan Dian, Ziqin Zhao, Ting Su, Hui Zhang, A-Mei Liu, Jingying Huang, Baoyang Zhang, Yifan Xia, Xueshan Wang, Binghui Qin, Weihong Kunming Univ Sci & Technol Fac Life Sci & Technol Kunming 650093 Yunnan Peoples R China First Peoples Hosp Yunnan Prov Obstet Dept Clin Lab Gynecol Dept Kunming Yunnan Peoples R China Ruili Entry Exit Inspect & Quarantine Author Clin Lab Ruili Yunnan Peoples R China Care Ctr Int Travel Hlth Yunnan Clin Lab Kunming Yunnan Peoples R China

Sexual transmission is currently the main mode of transmission of the human immunodeficiency virus (HIV). In this study, 181 HIV-infected female cross-border travelers entering Yunnan province were recruited between 2003 and 2012. HIV RNAs were extracted from their frozen serum and gag-pol gene sequences were obtained for phylogenetic and recombination analyses. In total, 131 gag-pol gene sequences were obtained successfully, at a rate of 72.4%. The most prevalent subtypes were CRF01_AE, followed by CRF08_BC, subtypes B and C. The other four subjects were classified as undefined subtypes and other recombinants. The subtype distribution of intravenous drug users was significantly different from that of sexually transmitted infections and unknown groups. The genetic distances of subtype B, C, and CRF01_AE strains were all close to the reference sequences from Yunnan province and Southeast Asian countries. Gene diversity and cocirculation of multiple subtypes were observed in female cross-border travelers, and CRF01_AE was the dominant epidemic subtype. The advantages of these subtype preferences for sexual transmission were obvious in HIV infection and transmission among this population. Our findings also suggest that close attention should be given to the HIV infection status of the female migrant population. In addition, a description of their epidemic characteristics is significant for the surveillance and prevention of acquired immunodeficiency syndrome in the Yunnan province.

关键词： acquired immunodeficiency syndrome cross‐ border travelers epidemic human immunodeficiency virus sexual transmission

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Identification and assessment of novel dynamic biomarkers for monitoring non-traumatic osteonecrosis of the femoral head staging

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ClinICAL AND TRANSLATIONAL MEDICINE 2023年第6期13卷 e1295页

作者： Jia, Yan Zhang, Yanqiong Li, shuwen Li, Ruihan Li, Weijie Li, Taixian Wang, Rongtian Huang, Guangrui He, Haijun lin, Na Chen, Weiheng Beijing Univ Chinese Med Affiliated Hosp 3 Dept Minimally Invas Arthrol Beijing Peoples R China China Acad Chinese Med Sci Inst Chinese Mat Med Res Ctr TCM Theory & Mat Med Literature Beijing Peoples R China Beijing Univ Chinese Med Sch Life Sci Beijing Peoples R China Wangjing Hosp China Acad Chinese Med Sci Dept Orthopaed 3 Beijing Peoples R China Beijing Univ Chinese Med Affiliated Hosp 3 51 Anwai Xiaoguanjie Beijing 100029 Peoples R China China Acad Chinese Med Sci Inst Chinese Mat Med Beijing 101121 Peoples R China

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Antibacterial photodynamic peptides for staphylococcal skin infection

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BIOMATERIALS SCIENCE 2020年第23期8卷 6695-6702页

作者： Lei, Xiaoling Qiu, lin Lan, Min Du, Xuancheng Zhou, shuwen Cui, Pengfei Zheng, Ronghui Jiang, Pengju Wang, Jianhao Xia, Jiang Changzhou Univ Sch Pharm Changzhou 213164 Jiangsu Peoples R China Shandong Univ Sch Phys Inst Adv Interdisciplinary Sci Jinan 250100 Peoples R China Changzhou Sun Pharmaceut Co Ltd Changzhou 213125 Jiangsu Peoples R China Chinese Univ Hong Kong Dept Chem Shatin Hong Kong Peoples R China

As the barrier between the human body and the outside world, the skin is vulnerable to pathogenic microorganisms, especially when suffering from skin injuries such as burns. Staphylococcus aureus remains the most common type of bacteria that infects humans, and the surging drug resistance poses a major threat to the treatment of these infections. Here we report the development of antibacterial photodynamic peptides (APPs) that are constructed based on the covalent conjugation of an antibacterial peptide and the photosensitizer chlorin e6 (Ce6). Peptide conjugation significantly increases the photo-stability of Ce6, while retaining its ROS generation capability under photo-irradiation. The APPs combine the antibacterial activity of the peptide and the photodynamic therapy of Ce6, and under the assistance of mild laser irradiation, can eradicate bacterial infection and inhibit the formation of bacterial biofilms ex vivo. One of the APPs, (GKRWWKWWRR)(2)KGGK(Ce6)G, AMP(2)-Ce6, with Ce6 conjugated with the dimeric peptide, showed exceptional antibacterial activity with an MIC90 value around 3.2 mu M without photo-irradiation and <0.1 mu M with short light treatment. Supported by a hydrogel matrix composed of gelatin and recombinant human collagen III protein (rhCol III) mimicking the extracellular matrix of skin cells, AMP(2)-Ce6 efficiently accelerated the healing rate of wounds and improved the quality of wound healing in mice infected with Staphylococcus aureus. Altogether, here we report the development of antibacterial photodynamic peptides, which together with a regenerative matrix material exhibit an added effect against staphylococcal skin infection. This composite material holds promise as a new type of wound dressing material for skin infection and wound healing.

关键词： Peptides

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高反射高隔热激光防护涂层制备及其防护性能

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材料保护 2024年第12期57卷 84-95页

作者：李书文吴凌峰杨冠军陈林西安交通大学金属材料强度国家重点实验室陕西西安710049 上海空间推进研究所上海201100

激光武器进入实战应用阶段,空天武器的抗激光防护研究迫在眉睫。高能激光长时间辐照,造成如金属反射层氧化反射率降低、烧蚀层质量损失、隔热层韧性不足等问题。基于此,提出一种综合利用反射和隔热原理的复合型激光防护策略。通过调控... 详细信息

激光武器进入实战应用阶段,空天武器的抗激光防护研究迫在眉睫。高能激光长时间辐照,造成如金属反射层氧化反射率降低、烧蚀层质量损失、隔热层韧性不足等问题。基于此,提出一种综合利用反射和隔热原理的复合型激光防护策略。通过调控喷涂功率、喷涂距离等参数,采用超音速火焰喷涂技术和等离子喷涂技术分别制备了NiCoCrAlTaY粘结层、氧化钇稳定氧化锆(YSZ)面层的双层涂层,探究表面粗糙度与孔隙率对反射率的影响规律,并考察了涂层的激光防护性能。结果表明:喷涂功率或喷涂距离越大,涂层的层状效果越明显,断面孔隙越多。涂层表面粗糙度随喷涂距离的增大先降低后增加。去除二次反射结构后的涂层表面Ra=1.8μm,当Ra=0.5μm时,涂层中的楔形结构使涂层反射率相较于Ra=1.8μm时的反射率提升了约2.4%。指出层状孔隙是高反射楔形结构的关键,球形孔隙是激光吸收点,孔隙率14.7%的工艺参数(39 kW,100 mm)为最优。在激光烧蚀试验中,激光功率密度的增加,涂层裂纹逐渐增多,直至烧蚀孔洞,线烧蚀率近似抛物线增长。低激光功率密度条件下(<3800 W/cm^(2)),表层组织轻微变化,功率增大,表层裂纹增多加深,重凝区花瓣状组织增大;大功率激光密度(>8050 W/cm^(2))条件下,涂层表层部分剥离与烧蚀孔洞致使涂层失效。

关键词：激光防护涂层等离子喷涂反射率防护性能

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Lesch-Nyhan综合征家兔模型的建立及其表型分析

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遗传 2024年第5期46卷 408-420页

作者：资崯郑淑文宁丽蔺紫怡鹿璇席嘉慧高月周小青唐成程五邑大学药学与食品工程学院江门529000 广东省医学大动物模型重点实验室江门529000 五邑大学华南生物医药大动物模型研究院江门529000

Lesch-Nyhan综合征(Lesch-Nyhan syndrome,LNS)是一种先天性的嘌呤代谢缺陷病,次黄嘌呤鸟嘌呤磷酸核糖转移酶(hypoxanthine phosphoribosyltransferase,HPRT)是其主要致病基因,临床特征表现为尿酸分泌过多、痛风、肾结石及肾脏损伤等,... 详细信息

Lesch-Nyhan综合征(Lesch-Nyhan syndrome,LNS)是一种先天性的嘌呤代谢缺陷病,次黄嘌呤鸟嘌呤磷酸核糖转移酶(hypoxanthine phosphoribosyltransferase,HPRT)是其主要致病基因,临床特征表现为尿酸分泌过多、痛风、肾结石及肾脏损伤等,目前致病机制尚未被完全阐明且无有效治愈手段。动物模型在疾病致病机理研究和治疗方式探索中发挥着重要功能。本研究采用高效的CRISPR/Cas9基因编辑技术和显微注射的方式敲除家兔(Oryctolagus cuniculus)HPRT基因建立了LNS家兔模型,以期能更好的模拟该疾病的表型。首先针对兔HPRT基因第3外显子设计一条sgRNA,将体外转录的sgRNA和Cas9 mRNA共注射到兔受精卵中,注射后的胚胎移植到代孕母兔子宫中,待仔兔出生后对其基因型及表型进行鉴定与分析。共出生4只仔兔(分别编号为R1、R2、R3和R4),测序结果显示4只仔兔都产生了不同程度的基因修饰,基因编辑效率达100%,其中R4仔兔T载体测序结果在基因编辑靶点未检测到野生型序列。通过6-硫代鸟嘌呤(6-Thioguanine,6-TG)药物测试,证明R4仔兔HPRT酶活性缺失;肾组织HE染色发现4只仔兔表现出肾小球炎细胞浸润、集合管脱落等肾脏损失。本研究成功设计了1条能够敲除家兔HPRT基因的sgRNA,并采用CRISPR/Cas9基因编辑技术和显微注射方式成功构建了HPRT基因修饰家兔,为研究LNS综合征致病机制和治疗方式提供了新的非啮齿类动物模型。

关键词： Lesch-Nyhan综合征 HPRT 家兔

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Multienzyme detection and in-situ monitoring of enzyme activity by bending CE using quantum dots-based polypeptide substrate

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ELECTROPHORESIS 2020年第12期41卷 1103-1108页

作者： Qiu, lin Cui, Pengfei Zhu, Zhilan Xu, Mingyu Jia, Wenjing Sheng, Jingyu Ni, Xinye Zhou, shuwen Wang, Jianhao Changzhou Univ Sch Pharmaceut Engn & Life Sci Changzhou Jiangsu Peoples R China Nanjing Med Univ Affiliated Changzhou 2 Peoples Hosp Changzhou 213003 Jiangsu Peoples R China Changzhou Le Sun Pharmaceut Co Ltd Changzhou Jiangsu Peoples R China Jiangsu Univ Wujin Hosp Changzhou Jiangsu Peoples R China

Multienzyme detection and monitoring enzyme activity in situ are significant for the disease to diagnose. This study aims to develop a quantum dots (QDs)-based nanoprobe Cyanine5-DDDLEVLFQFPGLVPRGSGGHHHHHH-QDs (Cy5-LEVLVP-QD), which is able to detect two enzymes inside a bent capillary using CE. Cy5-LEVLVP and QDs were allowed to bind with each other through metal affinity interaction and then injected the Cy5-LEVLVP-QD complex into a capillary with different bends, followed by related enzyme that can cleave the Cy5-LEVLVP peptide. The fluorescence of Cy5 was excited by QDs due to Forster resonance energy transfer. By monitoring the peaks produced by the original Cy5-LEVLVP-QD complex and a significant fluorescence change, sensitive analysis of two different enzymes was conducted. Therefore, the novel approach of using capillaries with semicircular bends could prove particularly useful for enzyme investigating in disease.

关键词： CE enzyme Forster resonance energy transfer peptide quantum dots

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晶圆传输机械手的实现途径及其结构设计

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电子工业专用设备 2023年第5期52卷 74-80页

作者：杨树文李霖于朋扬李峥中国电子科技集团公司第四十五研究所北京100176

晶圆传输是集成电路制造设备的组成部分之一;研究晶圆传输核心部件机械手的实现途径和国内外的技术状况,分析晶圆传输机械手的更新和迭代,探讨传输精度和效率的优化措施。通过对应用技术和需求分析,对其机械结构和末端执行器进行逆向设... 详细信息

晶圆传输是集成电路制造设备的组成部分之一;研究晶圆传输核心部件机械手的实现途径和国内外的技术状况,分析晶圆传输机械手的更新和迭代,探讨传输精度和效率的优化措施。通过对应用技术和需求分析,对其机械结构和末端执行器进行逆向设计并对其性能进行测试,把设计固化为可以应用的技术成果;同时提出了晶圆传输机械手的优化方向,为后期技术升级和产业化提供了理论依据,其研究结果对同类设计具有借鉴意义。

关键词：半导体制造工艺与设备结构设计逆向设计机械手末端执行器晶圆传输

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Surface-Engineered Cubosomes Serve as a Novel Vaccine Adjuvant to Modulate Innate Immunity and Improve Adaptive Immunity in vivo

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INTERNATIONAL JOURNAL OF NANOMEDICINE 2020年 15卷 8595-8608页

作者： Liu, Zhenguang Yu, lin Gu, Pengfei Bo, Ruonan Xu, shuwen Wusiman, Adelijiang Liu, Jiaguo Hu, Yuanliang Wang, Deyun Nanjing Agr Univ Inst Tradit Chinese Vet Med Coll Vet Med Nanjing 210095 Peoples R China Nanjing Agr Univ MOE Joint Int Res Lab Anim Hlth & Food Safety Coll Vet Med Nanjing 210095 Peoples R China

Objective: Recent studies have revealed the adjuvant activity of cubosomes and their potential utility as an antigen delivery system. In this study, to further enhance the adjuvant activity of cubosomes, two cationic polymers are modified on the surface of cubosomes. Methods: Here, we exploit the effects of surface chemistry on the adjuvant activity of Ganoderma lucidum polysaccharide cubosomes (GLPC) by placing two kinds of molecules, that is, cetyltrimethylammonium bromide (CTAB) and poly(diallydimethyl ammonium chloride) (PDDAC), on their surface. Results: CTAB- or PDDAC-modified GLPC were found to significantly promote humoral and cellular immune responses, as well as the proliferation of CD3+ CD4+ or CD3+ CD8+T cells through the powerful activation of dendritic cells (DCs). The enhanced immune responses of PDDAC-modified GLPC might be attributed to the maturation of DCs into draining lymph nodes and the activation of spleen and cytokines in serum. Conclusion: PDDAC modification is beneficial for enhancing humoral and cellular immune response, suggesting that PDDAC-GLPC-OVA has the ability to be a potential adjuvant for vaccine.

关键词： cubosomes Ganoderma lucidum polysaccharide surface-engineering immune response RNA-seq

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