A super hydrophilic SiO2 aerogel (SHSA) was prepared using the sol-gel method and CO2 supercritical drying technology, yielding a specific surface area of 680.08 m2/g and a total pore volume of 3.95 cm3/g. The hydroph...
详细信息
By harvesting the flowing kinetic energy of water using a rotating triboelectric nanogenerator (R-TENG), this study demonstrates a self-powered wastewater treatment system that simultaneously removes rhodamine B (RhB)...
详细信息
By harvesting the flowing kinetic energy of water using a rotating triboelectric nanogenerator (R-TENG), this study demonstrates a self-powered wastewater treatment system that simultaneously removes rhodamine B (RhB) and copper ions through an advanced electrochemical unit. With the electricity generated by R-TENG, the removal efficiency (RE) of RhB can reach the vicinity of 100% within just 15 min when the initial concentration of RhB is around 100 ppm at optimized conditions. The removal efficiency of copper ions can reach 97.3% after 3 h within an initial concentration of 150 ppm at an optimized condition. Importantly, a better performance and higher treating efficiency are found by using the pulsed output of R-TENG than those using direct current (DC) supply for pollutant removal when consuming equal amount of energy. The recovered copper layer on the cathode through R-TENG is much denser, more uniform, and with smaller grain size (d = 20 nm) than those produced by DC process, which also hints at very promising applications of the R-TENG in electroplating industry. In light of the merits such as easy portability, low cost, and effectiveness, this R-TENG-based self-powered electrochemical system holds great potential in wastewater treatment and electroplating industry.
Estuarine and tidal wetlands with high primary productivity and biological activity play a crucial role in coastal nutrient dynamics. Here, to better reveal the effects of extracellular enzymes and microbial community...
详细信息
Estuarine and tidal wetlands with high primary productivity and biological activity play a crucial role in coastal nutrient dynamics. Here, to better reveal the effects of extracellular enzymes and microbial community on carbon (C) and nitrogen (N) mineralization, the incubation experiments with different C and N addition patterns to the tidal sediments of the Yangtze Estuary (China) were conducted. The results suggested a significant increase in cumulative CO2 effluxes in the C and CN treatment experiments, while no significant difference in cumulative CO2 effluxes between the N treatment and control (CK) experiments was observed. In addition, the nutrient addition patterns had a great influence on dissolve organic C and N levels, but a small effect on microbial biomass C and N. Microbial community composition and microbial activity were found to be positively correlated with organic C (OC) and the molar ratio of C to N (C/N). Partial correlation analysis, controlling for C/N, supported direct effects of OC on the activity of carbon-cycling extracellular enzymes (cellulase and polyphenol oxidase), while C/N exhibited negatively correlations with urease and Gram-positive bacteria to Gram-negative bacteria (G+/G-). Strong relationships were found between CO2 efflux and mineral nitrogen with the activity of specific enzymes (sucrase, cellulase, and polyphenol oxidase) and abundances of Gram-negative bacteria, arbuscular mycorrhizal fungi, and fungi, suggesting the significant influences of microbial community and enzyme activity on C and N mineralization in the estuarine and tidal wetlands. Furthermore, this study could highlight the need to explore effects of nutrient supply on microbial communities and enzyme activity changes associated with the C and N mineralization in these wetlands induced by the climate change.
Adipose-derived stem cells(ASCs)and mesenchymal stem cells are promising for tissue repair because of their multilineage differentiation *** previous data confirmed that the implantation of mixed ASCs and chondrocyt...
详细信息
Adipose-derived stem cells(ASCs)and mesenchymal stem cells are promising for tissue repair because of their multilineage differentiation *** previous data confirmed that the implantation of mixed ASCs and chondrocytes into cartilage defects induced desirable in vivo healing ***,the paracrine action of ASCs on chondrocytes needs to be further *** this study,we established a co-culture system to achieve cell-to-cell and cell-to-tissue crosstalk and explored the soluble growth factors in both ASCs and chondrocytes supplemented with 1%fetal bovine serum to mimic the physiological *** ASCs,we screened for growth factors by semi-quantitative PCR and quantitative real-time PCR and found that the expression of bone morphogenetic protein 2(BMP-2),vascular endothelial growth factor B(VEGFB),hypoxia inducible factor-1α(HIF-1α),fibroblast growth factor-2(FGF-2),and transforming growth factor-β1 significantly increased after co-culture in comparison with *** chondrocytes,VEGFA was significantly enhanced after ***,the expression of collagen II and aggrecan was significantly down-regulated in the co-culture group compared with the mono-culture ***,among all the growth factors screened,we found that the BMP family members BMP-2,BMP-4,and BMP-5 were down-regulated and that VEGFB,HIF-1α,FGF-2,and PDGF were significantly decreased after *** results suggest that crosstalk between ASCs and chondrocytes is a pathway through the regulated growth factors that might have potential in cartilage repair and regeneration and could be useful for tissue engineering.
Objectives: The aim of this study was to investigate gene expressions of growth factors for angiogenesis, in a three-dimensional (3D) gel populated with adipose-derived stromal cells (ASCs) and endothelial cells (ECs)...
Objectives: The aim of this study was to investigate gene expressions of growth factors for angiogenesis, in a three-dimensional (3D) gel populated with adipose-derived stromal cells (ASCs) and endothelial cells (ECs) in co-culture. Materials and methods: The 3D gel, mixed with green fluorescent protein (GFP)-positive ASCs and DsRed-Express-positive ECs, 1:1 ratio, was established in vitro. The phenomenon of angiogenesis was observed using confocal microscopy. To detect gene expressions for growth factor proteins in both ASCs and ECs, transwell co-culture was used, and cell lysate samples were collected at 1, 3, 5 and 7 days. Semi-quantitative polymerase chain reaction (PCR) was conducted to quantify mRNA expressions of the growth factors. Results: Angiogenesis was first observed in the gels by 7 days post-implantation. Over this time in ECs, genes coding for VEGFA/B, IGF-1, HIF-1 alpha, FGF-1/-2 and BMP-5/-7 significantly increased. Meanwhile in ASCs, genes coding for VEGFA/B, IGF-1, HIF-1 alpha, FGF-1/-2 and BMP-6 also were significantly enhanced. In particular, increased amounts of IGF-1 and HIF-1 alpha in both ECs and ASCs were prominent relative to other factors. Conclusions: Contact co-culture with ASCs and ECs at 1:1 ratio, in the 3D gel promoted angiogenesis;non-contact co-culture further confirmed gene expressions for growth factors, VEGFA/B, IGF-1, HIF-1 alpha and FGF-1/-2 in both ASCs and ECs;BMP-5/-7 in ECs and BMP-6 in ASCs were also confirmed. This establishment of growth factor expression seemed to be responsible for enhancement of angiogenesis. This indicates that these factors could be utilized as targets for engineered angiogenesis.
The management of chondral defects is a challenging topic of current interest for scientists and surgeons, which has a crucial impact on human cost. Even after several centuries after its first observation, this probl...
详细信息
The management of chondral defects is a challenging topic of current interest for scientists and surgeons, which has a crucial impact on human cost. Even after several centuries after its first observation, this problem has still not found a satisfactory and definitive answer. Cartilage tissue engineering, which involves novel natural scaffolds, has emerged as a promising strategy for cartilage regeneration and repair. In this study, bio-plasticpoly-3-hydroxybutyrate-4-hydroxybutyrate (P34HB) film was first fabricated. The characteristics of P34HB film were tested using SEM and AFM. Cell morphologies on P34HB film were obtained using SEM and fluorescence microscopy after cell seeding. The tests of cell adhesion and proliferation on P34HB film were conducted using MTT and CCK-8 assays, respectively. Furthermore, full cartilage defects in rats were created and P34HB films were implanted to evaluate their healing effects within 8 weeks. It was found that P34HB film, as a biomaterial implant, possessed good in vitro properties for cell adhesion, migration, and proliferation. Importantly, in the in vivo experiment, P34HB film exhibited desirable healing outcomes. These results demonstrated that P34HB film was a good scaffold for cartilage tissue engineering for improving cell proliferation and adhesion.
Moire pattern can be readily observed in the case of superimposing two periodic structures. Recently, moire pattern, which degrades the quality of an image, become unavoidable in many LED display and three-dimensional...
详细信息
ISBN:
(纸本)9781628417999
Moire pattern can be readily observed in the case of superimposing two periodic structures. Recently, moire pattern, which degrades the quality of an image, become unavoidable in many LED display and three-dimensional displays, which consist of regular structures. Therefore, a model of moire which fit the character of human vision is important to solve the problem of image quality degradation. In this paper, we modified the the model of moire which proposed by Amidror to evaluate the color moire fringes, which consider the property of human vision system of contrast sensitivity. From the modified model we conclude that moire minimization could have two lattice form an certain angle. The experimental results show that moire pattern are vivid when the angle is 0 degrees, 15 degrees, 20 degrees and 45 degrees. However, the spatial frequency of moire in 10 degrees, 25 degrees, 30 degrees and 35 degrees are fine in spatial domain. It is also reveal that changing angle is simple and effective in alleviating remarkably moire pattern.
暂无评论