Psychophysical experiments have been carried out in which the color appearance of high-gloss and low-gloss color printing atlases was assessed using a magnitude estimation method under three different light sources. A...
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Psychophysical experiments have been carried out in which the color appearance of high-gloss and low-gloss color printing atlases was assessed using a magnitude estimation method under three different light sources. A linearity fitting algorithm by the method of least squares was applied to evaluate the correlation between visual experiment data and predicted results through the CIECAM02 model of different glossinesses of color printing atlases. The research result has shown that there was a good correlation between mean visual results and predicted results for hue and lightness of two distinct glossinesses of the color printing atlas. The correlations of hue and lightness between visual experiment data and predicted results were the best and the second best, respectively, under the same light source. Colorfulness was the worst. Meanwhile, high-gloss and low-gloss color printing atlases have been estimated and compared in the viewing conditions of 0 degrees/45 degrees. The result has indicated that the hue of the color printing atlas could not be affected by gloss levels under three light sources. Colorfulness of the color printing atlas almost could not be affected by gloss levels under D65 and TL84 light sources. Lightness of the color printing atlas could be affected by gloss levels under a TL84 light source. (c) 2014 Optical Society of America
Tea tree is the general term for many species of melaleuca plants. The essential oil or liquid extracted from Australian tea tree can be added to cosmetics and care products. The experimental parameters of solvent ext...
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Tea tree is the general term for many species of melaleuca plants. The essential oil or liquid extracted from Australian tea tree can be added to cosmetics and care products. The experimental parameters of solvent extraction are the key factors influencing the antioxidant activity of Australian tea tree. This study used the orthogonal array of the Taguchi method to plan the experiment. The quality data obtained from experiment were analyzed by the factor effects of the Taguchi method to find the significant experimental parameters influencing the antioxidant activity of Australian tea tree, and obtain the optimal experimental parameter combination of solvent extraction for antioxidant activity. Finally, the reliability of the experiment was validated by a confirmation experiment. According to the experimental results, this method was applicable to the optimization problem of the experimental procedure. The optimization can be implemented for objective numerical analysis operation, saving on experimental consumables, time and cost.
Biofunctionalized quantum dots (QDs), especially protein-coated QDs, are known to be useful targeted fluorescent labels for cellular and deep-tissue imaging. These nanoparticles can also serve as efficient energy dono...
Biofunctionalized quantum dots (QDs), especially protein-coated QDs, are known to be useful targeted fluorescent labels for cellular and deep-tissue imaging. These nanoparticles can also serve as efficient energy donors in fluorescence resonance energy transfer (FRET) binding assays for the multiplexed sensing of tumor markers. However, current preparation processes for protein-functionalized QDs are laborious and require multiple synthesis steps (e.g. preparing them in high temperature, making them dispersible in water, and functionalizing them with surface ligands) to obtain a high quality and quantity of QD formulations, significantly impeding the progress of employing QDs for clinical diagnostics use such as a QD-based immunohistofluorescence assay. Herein, we demonstrate a one-step synthesis approach for preparing protein-functionalized QDs using a microfluidic (MF) chip setup. Using bovine serum albumin (BSA) molecules as the surface ligand model, we first studied and optimized the MF reaction synthesis parameters (e.g. reaction temperature, and channel width and length) for making protein-functionalized QDs using COMSOL simulation modeling, followed by experimental verification. Moreover, in comparison with the BSA-functionalized QDs synthesized using the conventional bench-top method, BSA-QDs prepared using the MF approach exhibit a significantly higher protein-functionalization efficiency, photostability and colloidal stability. The proposed one-step MF synthesis approach provides a rapid, cost effective, and a small-scale production of nanocrystals platform for developing new QD formulations in applications ranging from cell labeling to biomolecular sensing. Most importantly, this approach will considerably reduce the amount of chemical waste generated during the trial-and-error stage of developing and perfecting the desired physical and optical properties of new QD materials.
Several dibenzocyclooctatetraene derivatives (5-7) and related biphenyls (8-11) were designed, synthesized, and evaluated for inhibition of cancer cell growth and the NF-kappa B signaling pathway. Compound 5a, a diben...
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Several dibenzocyclooctatetraene derivatives (5-7) and related biphenyls (8-11) were designed, synthesized, and evaluated for inhibition of cancer cell growth and the NF-kappa B signaling pathway. Compound 5a, a dibenzocyclooctatetraene succinimide, was discovered as a potent inhibitor of the NF-kappa B signaling pathway with significant antitumor activity against several human tumor cell lines (GI(50) 1.38-1.45 mu M) and was more potent than paclitaxel against the drug-resistant KBvin cell line. Compound 5a also inhibited LPS-induced NF-kappa B activation in RAW264.7 cells with an IC50 value of 0.52 mu M, prevented I kappa B-alpha degradation and p65 nuclear translocation, and suppressed LPS-induced NO production in a dose-dependent manner. The antitumor data in cellular assays indicated that relative positions and types of substituents on the dibenzocyclooctatetraene or acyclic biphenyl as well as torsional angles between the two phenyls are of primary importance to antitumor activity. (C) 2013 Elsevier Ltd. All rights reserved.
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