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Calcium lactate as a soil amendment: Mechanistic insights into its effect on salinity, alkalinity, and aggregation in saline-alkaline soils

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SOIL & TILLAGE RESEARCH 2025年 248卷

作者： Fan, Jingbiao Lv, Qilin Zhou, Tairan Wang, Tianhao Gao, Haixiang Zhou, Wenfeng ren, Xueqin Hu, shuwen China Agr Univ Coll Resources & Environm Sci 2 Yuanmingyuan West Rd Beijing 100193 Peoples R China China Agr Univ Coll Sci 2 Yuanmingyuan West Rd Beijing 100193 Peoples R China China Agr Univ Coll Resources & Environm Sci China Beijing Key Lab Farmland Soil Pollut Prevent 2 Yuanmingyuan West Rd Beijing 100193 Peoples R China

Soil salinization is a problem that threatens agricultural productivity and food security. It occurs when soluble salts, sodium (Na+) and carbonate (CO32-) ions accumulate excessively in the soil. In this study we thoroughly investigate the potential of using calcium lactate (CL) as a soil amendment to mitigate the effects caused by soil salinization. We comprehensively analyze how CL affects soil parameters such, as salinity, colloid morphology and physicochemical properties. Our hypotheses are that CL can effectively counterbalance the adverse soil pH and high salinity conditions in the soil. This happens because CL reacts with free CO 3 2- ions through an acid base neutralization reaction, which helps reduce the alkalinity of the soil. Moreover, this alteration enhances the ion exchange process between calcium (Ca2+) and sodium ions (Na+), resulting in the substitution of Ca2+ ions for Na+ ions attached to the soil particle surfaces. As a result, there is a decrease in the percentage of sodium leading to an improvement in saline soil conditions. Moreover, CL supports the formation of more stable soil aggregates, by bridging and hydrogen bonding processes thereby enhancing soil structure. Furthermore, it promotes the creation of organic mineral composites. Increases storage of carbon in the soil ultimately improving its fertility. When we compare CL to inorganic calcium salts such as CaCl2 and gypsum it becomes apparent that CL is more environmentally friendly, safe and effective, as a soil amendment. Its main purpose is to mitigate soil salinity and improve soil quality.

关键词： Soil colloid Calcium lactate Soil amendment Saline-alkali soils Aggregation

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Facile synthesis of N-doped carbon dots for direct/indirect detection of heavy metal ions and cell imaging

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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 2021年第16期28卷 19878-19889页

作者： Xu, Zijun Liu, Jin Wang, Kejia Yan, Binwei Hu, shuwen ren, Xueqin Gao, Zideng China Agr Univ Coll Resources & Environm Sci 2 Yuanmingyuan West Rd Beijing 100193 Peoples R China Beijing Key Lab Farmland Soil Pollut Prevent & Re Beijing 100193 Peoples R China

In this study, an approach for the facile, rapid, energy-saving, and sensitive determination of Fe3+ and Cr(VI) was developed. L-arginine/D-glucose carbon quantum dots (Arg/Glu-CQDs), with a photoluminescence quantum yield of 21%, were synthesized from L-arginine and D-glucose through a facile, hydrothermal process. The maximum emission wavelength of Arg/Glu-CQDs was observed at 450 nm, under an excitation wavelength of 365 nm. In addition, Arg/Glu-CQDs exhibited a sensitive and selective response to Fe3+ compared to Fe2+ and other metal ions. The Arg/Glu-CQDs' fluorescence was noticeably quenched through the inner filter effect (IFE) when Arg/Glu-CQDs were mixed with Fe3+. Accordingly, the Arg/Glu-CQDs/Fe2+ system could selectively detect Cr(VI);Cr(VI) could oxidize Fe2+ to Fe3+ and quench the fluorescence. The fluorescence sensor system (i.e., the Arg/Glu-CQDs/Fe2+ system) showed high sensitivity and excellent selectivity for the detection of Fe3+ and Cr(VI) in river water samples. Satisfactory detection efficiencies ranging from 97.07 to 103.46% were obtained. The cytotoxicity of Arg/Glu-CQDs was evaluated through an MTT assay using A549 cells as the target, to extend the application of Arg/Glu-CQDs to biological systems;the MTT assay indicated that the Arg/Glu-CQDs is non-cytotoxicity. Arg/Glu-CQDs were also successfully imaged in A549 cells indicating further application possibilities in bioimaging.

关键词： Carbon dots Fe3+ Cr(VI) Cellular imaging Cellular toxicity Facile synthesis

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Cretaceous Evolution of the Northeastern Asian Continent: Insights From the Pikou Strike-Slip Fault Zone of the Liaodong Peninsula, NE China

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TECTONICS 2025年第6期44卷

作者： ren, Zhiheng Dong, shuwen Xiao, Wenjiao Sang, Miao Zhang, Yueqiao Chen, Xuanhua Shi, Wei Zhang, Yu Chinese Acad Sci Xinjiang Inst Ecol & Geog State Key Lab Ecol Safety & Sustainable Dev Arid L Urumqi Peoples R China Xinjiang Key Lab Mineral Resources & Digital Geol Urumqi Peoples R China Chinese Acad Geol Sci SinoProbe Lab Beijing Peoples R China Nanjing Univ State Key Lab Crit Earth Mat Cycling & Mineral Dep Nanjing Peoples R China

Clarifying the Cretaceous evolution of the NE Asian continent has always been a key issue in unraveling the intracontinental responses of overriding plate to the subduction of Paleo-Pacific plate during the past decades. However, the structural details and precise timing of the transition from the Early Cretaceous extension to the Late Cretaceous shortening are still poorly constrained. Here we present structural and geochronological data about the Pikou strike-slip fault zone of the southern Liaodong Peninsula, where the Liaonan-Wanfu metamorphic core complex (MCC) is well exposed, to decipher this vital tectonic transition. Field observations and structural analysis show that the Pikou strike-slip fault zone is a sinistral transpressional fault zone that reworked the Liaonan-Wanfu MCC. Inversion of fault-slip data indicates that the brittle sinistral faulting was a result of NW-SE compression. Geochronological constraints suggest that the Pikou strike-slip fault zone was initiated as the high-angle brittle normal faults at ca. 102 Ma, and then transformed into a sinistral transpressional fault zone in the early Late Cretaceous. Our new data, together with the regional multidisciplinary data, further confirm that the tectonic transition occurred at ca. 87 Ma. Thereby the Cretaceous evolution of the NE Asian continent since 135 Ma can be modified as the significant NW-SE extension of 135-102 Ma, the gradually weakening extension of 102-87 Ma and the NW-SE compression of 87-79 Ma. Their geodynamics were controlled by the alternating slab rollback and low-angle subduction of the Paleo-Pacific plate.

关键词： strike-slip fault zone structural analysis Cretaceous evolution Liaodong Peninsula NE Asian continent

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EuHDZ25 positively affects rubber biosynthesis by targeting EuFPS1 in Eucommia leaves

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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2024年第Part1期272卷 132707页

作者： Zhang, shuwen ren, Yongyu Wang, Shun Song, Lianjun Jing, Yanchun Xu, Tingting Kang, Xiangyang Li, Yun Beijing Forestry Univ Coll Biol Sci & Technol Natl Engn Res Ctr Tree Breeding & Ecol Restorat State Key Lab Tree Genet & Breeding Beijing 100083 Peoples R China Henan Agr Univ Coll Forestry Zhengzhou 450046 Peoples R China Weixian Forestry Cultivat Base Super Species Weixian Eucommia Natl Forest Tree Germplasm Reposi Hebei Peoples R China 35 Qinghua East Rd Beijing Peoples R China

Eucommia ulmoides is a temperate gum source plant that produces trans-polyisoprene (TPI), also known as Eucommia rubber. The structural configuration and function of TPI offer a new material with important potential for industrial development. In this study, we detected the TPI content in the leaves of diploid and triploid E. ulmoides plants. The average TPI content in the leaves of triploid E. ulmoides was significantly higher than that of diploid. Transcriptome data and weighted gene co-expression network analyses identified a significant positive correlation between the EuFPS1 gene and TPI content. Overexpression of EuFPS1 increased the density of rubber particles and TPI content, indicating its crucial role in TPI biosynthesis. In addition, the expression of EuHDZ25 in E. ulmoides was significantly positively correlated with EuFPS1 expression. Yeast one-hybrid and dual-luciferase assays demonstrated that EuHDZ25 mainly promotes TPI biosynthesis through positive regulation of EuFPS1 expression. The significantly up-regulated expression of EuHDZ25 and its consequent upregulation of EuFPS1 during the biosynthesis of TPI may partially explain the increased TPI content of triploids. This study provides an important theoretical foundation for further exploring the molecular mechanism of secondary metabolites content variation in polyploids and can help to promote the development and utilization of rubber resources.

关键词： EuHDZ25 EuFPS1 Triploid Eucommia rubber Transcriptional regulation Co -expression network

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Bioinspired Macrocyclic Molecule Supported Two-Dimensional Lamellar Membrane with Robust Interlayer Structure for High-Efficiency Nanofiltration

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ADVANCED SCIENCE 2023年第5期10卷 e2206516页

作者： Zhang, Pengcheng Zhang, Yujuan Wang, Lin Qiu, Kaikai Tang, Xiaoyi Gibson, John K. Liu, Xue Mei, Lei An, shuwen Huang, Zhiwei ren, Peng Wang, Yi Chai, Zhifang Shi, Weiqun Chinese Acad Sci Inst High Energy Phys Lab Nucl Energy Chem Beijing 100049 Peoples R China Lanzhou Univ Sch Nucl Sci & Technol Radiochem Lab Lanzhou 730000 Peoples R China Chinese Acad Sci Ningbo Inst Mat Technol & Engn Engn Lab Adv Energy Mat Ningbo 315201 Peoples R China Univ Sci & Technol Beijing Sch Mat Sci & Engn Beijing 100083 Peoples R China Lawrence Berkeley Natl Lab Chem Sci Div Berkeley CA 94720 USA Xi An Jiao Tong Univ State Key Lab Mech Behav Mat Xian 710049 Peoples R China East China Univ Technol Sch Nucl Sci & Engn Nanchang 330013 Peoples R China State Key Lab NBC Protect Civilian Beijing 102205 Peoples R China

2D lamellar membranes (2DLMs) are used for efficient desalination and nanofiltration. However, weak interactions between adjacent stacked nanosheets result in susceptibility to swelling that limits practical applicability. Inspired by the super adhesion of multi-point suction cups on octopus tentacles, a 2DLM is constructed from Ti3C2Tx MXene supported by the macrocyclic "multi-point" molecule cucurbit[5]uril (CB5) and demonstrated for nanofiltration of methyl blue (MB) and enrichment of uranyl carbonate. Experimental results and density functional theory calculations indicate that CB5 rivets to the surface of the nanoflakes through strong stable interactions between its multiple binding sites and surface hydroxyl functional groups on MXene nanosheets. This novel 2DLM exhibits excellent nanofiltration performance (69 L m(-2)h(-1)bar(-1) permeance with 93.6% rejection for MB) and can be recycled at least 30 times without significant degradation. The 2DLM exhibits excellent swelling resistance at high salinity, with a demonstration of selective enrichment of uranyl carbonate from artificial water and natural seawater. The results provide a new strategy for constructing highly stable 2DLMs with interlayer spacing controllable from sub-nano to nanometer scales, for size-selective sieving of molecules and ions, high-efficiency nanofiltration, and other applications.

关键词： membrane separation MXene nanofiltration size sieving uranium enrichment

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Foodomics uncovers functional and volatile metabolite dynamics in red raspberry chewable tablet optimized processing

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FOOD CHEMISTRY 2024年 450卷 139379页

作者： Song, Yangbo ren, Xiaoli Zhao, Lili Zhang, Biying Chi, Wei Liu, Yanlin Shi, Kan Liu, shuwen Qinghai Univ Agr & Anim Husb Coll Xining 810086 Peoples R China Northwest A&F Univ Coll Enol Yanglin 712100 Peoples R China Northwest A&F Univ Ningxia Helan Mt East Foothill Wine Expt & Demonst Yongning 750104 Ningxia Peoples R China Shaanxi Engn Res Ctr Viti Viniculture Xianyang 712100 Peoples R China

Raspberries are known to contain valuable metabolites and possess a robust antioxidant capacity. However, the impact of different tablet processing stages on the nutritional content and flavor profile of raspberries remains unclear. The dynamic profile of functional and volatile metabolites was investigated through foodomics combined with UPLC-MS/MS-based widely targeted metabolomics and HS-SPME-GC-MS, and antioxidant capacities were assessed during tablet processing. 1336 functional metabolites and 645 volatile metabolites were identified. Results indicated tablets retained 34% similar to 61% of the total volatile contents. In addition, the conversion intensity of functional metabolites was consistent with the order of "Tableting > Freeze-drying > Crushing". Compared to raspberry, tablets showed higher antioxidant activity, which was positively correlated with vitamin contents. This study elucidated that tablet formation demonstrated advantages in antioxidation and aroma retention, which may provide insights for enhancing quality during the tableting process.

关键词： Widely targeted metabolomics HS-SPME-GC-MS Antioxidant activity Plateau red raspberry Processing stages

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Prioritizing microbial functions over soil quality for enhanced multifunctionality in saline-sodic soil remediation

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JOURNAL OF ENVIRONMENTAL MANAGEMENT 2025年 379卷 124731页

作者： Zhou, Tairan Zhang, Luxin Yang, Xu Wu, Zeen Yang, Ziye Wang, Jie Chen, Ning ren, Xueqin Hu, shuwen China Agr Univ Coll Resources & Environm Sci Beijing Peoples R China Natl Ctr Technol Innovat Comprehens Utilizat Salin Dongying Shandong Peoples R China Lanzhou Univ Coll Ecol State Key Lab Herbage Improvement & Grassland Agro 222 Tianshui South Rd Lanzhou 730000 Gansu Peoples R China Lanzhou Univ Yuzhong Mt Ecosyst Observat & Res Stn Lanzhou 730000 Gansu Peoples R China

Paddy cultivation has become a widely adopted approach for saline-sodic wasteland reclamation, aiming to mitigate the food crisis and enhance soil quality. Nevertheless, the impact of long-term paddy cultivation on the interplay between soil quality, microbial metabolic functions, and soil ecosystem multifunctionality (EMF) remains unclear. Here, we evaluated soil physicochemical properties, the abundance of 132 biomarker functional genes, and soil EMF across a 78-year period of saline-sodic paddy cultivation. After 78 years of paddy cultivation, soil pH and electrical conductivity (EC) decreased by 43.38% and 93.02% compared to saline-sodic wasteland (WL), respectively. Moreover, principal component analysis was used to select a minimal dataset of soil indicators and to establish a soil quality index (SQI). Significant positive correlations were observed between SQI and rice yield, implying that soil quality was the main factor driving increases in saline-sodic farmland. The Mantel test indicates that soil microbial biomass, SQI, and the availability of nutrients exhibit a significant positive relationship with the abundance and expression of genes related to carbon (C), nitrogen (N), and phosphorus (P) cycling, encompassing crucial biogeochemical processes like hemicellulose degradation, C fixation, N degradation, and organic P mineralization. This indicates that changes in soil physicochemical properties significantly affect biogeochemical cycling in saline-sodic soils. Differences in the abundance of microbial P core functional genes explained 41.9% of variation in soil EMF, followed by key soil physicochemical indicators (EC, available potassium, microbial biomass nitrogen, etc.) selected through random forest analysis. Further, we identified a key threshold for changes in soil EMF during long-term saline-sodic paddy cultivation, with EMF increasing for the first 20 years of restoration before decreasing thereafter. Finally, partial least squares path modeling

关键词： Reclamation Soil quality index Soil ecosystem multifunctionality Crop yield Salinization

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Aggregate stability and organic carbon stock under different land uses integrally regulated by binding agents and chemical properties in saline-sodic soils

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LAND DEGRADATION & DEVELOPMENT 2021年第15期32卷 4151-4161页

作者： Feng, Haojie Wang, Shunyi Gao, Zideng Pan, Hong Zhuge, Yuping ren, Xueqin Hu, shuwen Li, Chengliang China Agr Univ Coll Resources & Environm Sci Beijing 100193 Peoples R China Shandong Agr Univ Coll Resources & Environm Sci Natl Engn Lab Efficient Utilizat Soil & Fertilize Tai An 271018 Shandong Peoples R China China Agr Univ Beijing Key Lab Farmland Soil Pollut Prevent & Re Beijing Peoples R China Minist Land & Resources China Key Lab Agr Land Qual Beijing Peoples R China

Aggregate stability and soil organic carbon (SOC) are critical for maintaining soil structure and preserving soil productivity. Land use substantially affects the process of soil aggregate stabilization and SOC sequestration, yet the detailed mechanisms have not been well studied in degraded soil ecosystems (e.g., saline-sodic soils). Therefore, to interpret the effects of land-use changes on soil aggregate stability and SOC stocks in saline-sodic soils, soils were sampled from four major land-use areas (degraded meadow, artificial forest, sorghum field, and paddy field) in Northeast China. The results showed that artificial forest soils exhibited the greatest proportion of water-stable aggregate (>250 mu m) in 0-10 and 10-20 cm layers than sorghum and paddy field, which was mainly due to the absence of tillage disturbance. The highest SOC stock (59.79 Mg ha(-1)) was observed in paddy soils, indicating its greater SOC sequestration potential than those of other land uses. The variance partition analysis (VPA) revealed that soil parameters resulted in 85.1% variation regarding soil aggregate stability, in which the chemical characteristics (i.e., pH, EC, exchangeable Na+, and exchangeable sodium percentage) contributed 23.1%, binding agents (SOC, exchangeable Ca2+, and poorly crystalline Fe/Al oxides) contributed 20.6%, and their interactions contributed 39.5%. Binding agents directly participated in soil aggregation processes by forming clay-polyvalent metal complexes, while the chemical characteristics indirectly promoted soil aggregation via increasing various binding agents. Understanding the interactions between binding agents and clay are important for promoting soil aggregate stabilization in saline-sodic soil.

关键词： binding agents land use saline-sodic soils soil aggregates stability soil organic carbon stock

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Case Report:Optical Pumped Magnetometer Magnetocardiography as a Potential Method of Therapy Monitoring in Fulminant Myocarditis

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Cardiovascular Innovations and Applications 2024年第1期9卷 564-571页

作者： shuwen Yang Kun Yang Lijun Zhang Yanlong ren Linqi Liu Huan Zhang Lanxin Feng Zhao Ma Shu Zhou Yi He Chenchen Tu Xiantao Song Hongjia Zhang Department of Cardiology Beijing Anzhen HospitalCapital Medical UniversityBeijing 100029China Center for Cardiac Intensive Care Beijing Anzhen HospitalCapital Medical UniversityBeijing 100029China Department of Radiology Beijing Anzhen HospitalCapital Medical UniversityBeijing 100029China Department of Cardiac Surgery Beijing Anzhen HospitalCapital Medical UniversityBeijing 100029China

Background:Fulminant myocarditis(FM)is associated with high mortality and an unfavorable long-term ***,noninvasive,rapid diagnostic and monitoring methods for FM are *** summary:This report details the case of a patient diagnosed with FM through a comprehensive assessment involving typical clinical symptoms,laboratory analyses,echocardiographic evidence,and cardiac magnetic resonance(CMR)*** the patient underwent CMR,optical pumped magnetometer magnetocardiography(OPM-MCG)revealed abnormalities characteristic of substantial myocardial *** invasive coronary angiography,the patient’s coronary arteries were determined not to exhibit significant ***,a myocardial bridge was observed in the middle segment of the anterior descending branch of the left coronary *** a 7-month follow-up,the patient’s symptoms showed marked improvement,and the OPM-MCG eventually normalized,aligning with the findings indicated by the ***:OPM-MCG is a valuable tool for therapy monitoring in FMs,offering a safe,non-invasive,and highly effective test for ancillary diagnostics and long-term prognostic ***,MCG may potentially assist in clinical decision-making regarding the need for further interventions in patients with myocardial bridge.

关键词： Fulminant myocarditis Magnetocardiography Myocardial ischemia Myocardial bridge

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Identification and characterization of 5 walnut MYB genes in response to drought stress involved in ABA signaling

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PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS 2021年第6期27卷 1323-1335页

作者： Li, Dapei Peng, Shaobing Chen, shuwen Li, Ziyi He, Yi ren, Bin Yang, Guiyan Northwest A&F Univ Coll Forestry Lab Walnut Res Ctr Yangling 712100 Shaanxi Peoples R China Northwest A&F Univ Coll Forestry Key Lab Econ Plant Resources Dev & Utilizat Shaan Yangling 712100 Shaanxi Peoples R China

Walnut is a popular nut tree species and usually suffers from drought stress. However, little information is available on the mechanism of walnut responding to drought stress, resulting in lack of basic understanding for its resistance. In order to excavate more functional genes that can respond to stressors, and enrich the theoretical basis for walnut resistance, in this study, 5 MYB genes with complete ORFs were identified from J. regia and the basic bio-information as well as expression patterns in different tissues and response to drought and ABA stresses were confirmed using qRT-PCR assay. The results showed that 2 JrMYB genes belong to R1-MYB subfamily and 3 JrMYBs belong to R2R3-MYB, encoding the proteins from 212 to 362 aa in length. The phylogenetic analysis categorized proteins of 5 JrMYBs and 40 Arabidopsis AtMYBs into 10 subgroups. JrMYBs in the same subgroup exhibited significant similarities in the composition of conserved domains and motifs in amino acid sequences and exon/intron organization in DNA sequences. The results of qRT-PCR analysis revealed that JrMYB genes diversely expressed in various tissues. Moreover, the expression values of JrMYBs were upregulated or downregulated significantly under drought and ABA stresses. Most attractively, in contrast with suffering from drought stress alone, the treatments with drought and additional ABA greatly enhanced the transcript levels of JrMYBs. All these results suggested that JrMYB genes play a vital role in plant biological processes and drought as well as ABA stress response, and possibly perform as ABA-dependent drought response transcription factors in plant.

关键词： Walnut MYB transcription factors Drought stress ABA signaling

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